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Infectious Disease: Parasitic Diseases

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Total 81 results found since Jan 2013.

Knocking down of the DHFR-TS gene in Toxoplasma gondii using siRNA and assessing the subsequences on toxoplasmosis in mice.
Abstract Toxoplasma gondii (T. gondii), an obligatory intracellular parasite, is the etiologic agent of toxoplasmosis. Dihydrofolate reductase-thymidylate synthase (DHFR-TS) is one of the most important enzymes in toxoplasma folic acid cycle. Due to the emergence of resistance in RH strain of T. gondii against pyrimethamine that acts via DHFR-TS inhibition and also the crucial role of small interference RNA (siRNA) technology in gene silencing, we aimed to use siRNA to knock down DHFR-TS gene expression in T. gondii as a therapeutic target against toxoplasmosis in a mouse model. Based on the DHFR-TS gene sequence,...
Source: Acta Tropica - April 7, 2020 Category: Infectious Diseases Authors: Azimi-Resketi M, Eskandarian A, Ganjalikhani-Hakemi M, Zohrabi T Tags: Acta Trop Source Type: research

Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research

siRNA ‐loaded liposomes: inhibition of encystment of Acanthamoeba and toxicity on the eye surface
This article is protected by copyright. All rights reserved. Glycogen phosphorilase, a key enzyme for encystment of Acanthamoeba,was silenced PEGylated cationic liposomes were used as carriers for siRNA delivery Ther was a significant reduction in the encystment of AP2 trophozoties
Source: Chemical Biology and Drug Design - January 29, 2017 Category: Biology Authors: Kathrin Faber, Giovanni K. Zorzi, Nathaly T. Brazil, Marilise B. Rott, Helder F. Teixeira Tags: Research Article Source Type: research

Silencing the target rhoptry neck protein (RON) gene responsible for the invasion of Toxoplasma gondii with siRNA transfection.
Authors: Yürük M, Aksoy T, Sivcan E, Nergiz H Abstract Toxoplasma gondii is a coccidian protozoan that causes toxoplasmosis is a common disease in Turkey as well as all over the world. It causes various clinical symptoms depending on the immune system status, age, or location of the disease. There is an organelle called the apical complex at the anterior end of the parasite. Rhoptry Neck Proteins (RONs), a component of this organelle, play a critical role in the formation of "moving junction" and parasitophorous vacuoles during host cell invasion. On the other hand, interfering RNA (iRNA) treatment options develo...
Source: Mikrobiyoloji Bulteni - January 27, 2019 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

Modeling Modulation of the Tick Regulome in Response to Anaplasma phagocytophilum for the Identification of New Control Targets
Conclusion Our modeling of the modulation of the tick regulome in response to A. phagocytophilum infection provided new insights into the mechanisms that target specific functions in different tick tissues. These results supported the use of network analysis for the study of regulome response to infection. Although general mechanisms affected by A. phagocytophilum infection may be conserved even between tick and human cells (de la Fuente et al., 2016b), the effect of vector-pathogen co-evolution on pathogen isolates adaptation to grow in tick cells (Alberdi et al., 2015) may result in differences between isolates in the m...
Source: Frontiers in Physiology - April 17, 2019 Category: Physiology Source Type: research

Auranofin, an Anti-rheumatic Gold Drug, Aggravates the Radiation-Induced Acute Intestinal Injury in Mice
Conclusion In this study, we found that a non-toxic dose of auranofin significantly aggravated the severity of the radiation-induced intestinal injury. This suggests that auranofin treatment can be an independent factor that influences the risk of intestinal complications after pelvic or abdominal radiotherapy. Ethics Statement All the protocols used in this study were approved by the Institutional Animal Care and Use Committee of the Korean Institute of Radiological and Medical Sciences (IACUC permit number: KIRAMS217-0007). Author Contributions H-JL, JS, and Y-BL designed the experiments. EL and JK conducted the exp...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research

Functions of the Vasa gene in Schistosoma japonicum as assessed by RNA interference.
Abstract Vasa, an enzyme belonging to the helicase family, contributes to the regulation of reproductive system development in many species. Thus, we hypothesized that the Vasa3 gene may function in the reproductive system of the parasite Schistosoma japonicum (S. japonicum), which is a major causative agent of schistosomiasis. It is a severe disease globally affecting humans and animals. To test this hypothesis, we firstly conducted whole mount in situ hybridization analyses and found that the S. japonicum Vasa3 (SjVasa3) gene was expressed mainly in the reproductive organs. We then explored the reproductive func...
Source: Gene - September 27, 2017 Category: Genetics & Stem Cells Authors: He S, Zhu L, Liu F, Liu Q, Shao Y, Hua M, Ding H, Shao W, Du Y, Hou X, Ren C, Liu M, Shen J Tags: Gene Source Type: research

Inhibitory effects and analysis of RNA interference on thioredoxin glutathione reductase expression in Schistosoma japonicum.
Abstract Abstract Schistosomes infect around 280 million people worldwide. The worms survive in the veins of the final host, where thioredoxin glutathione reductase (TGR) activity helps the parasites to survive in the aerobic environment. In the present study, we synthesized 2 small interfering RNAs (siRNA S1 and S2) targeting the Schistosoma japonicum (Sj) TGR gene, and used them to knockdown the TGR gene. The knockdown effects of the siRNAs on SjTGR and the thioredoxin reductase (TrxR) activity of SjTGR were evaluated in vitro and in mice infected with schistosomes. The results of transfection with the siRNAs vi...
Source: The Journal of Parasitology - March 15, 2014 Category: Parasitology Authors: Han Y, Fu Z, Hong Y, Zhang M, Han H, Lu K, Yang J, Xiangrui LI, Lin J Tags: J Parasitol Source Type: research

BLT1-mediated O-GlcNAcylation is required for NOX2-dependent migration, exocytotic degranulation and IL-8 release of human mast cell induced by Trichomonas vaginalis-secreted LTB4
Publication date: Available online 31 May 2018 Source:Microbes and Infection Author(s): Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon Shin Trichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of intracel...
Source: Microbes and Infection - June 1, 2018 Category: Infectious Diseases Source Type: research

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence
In conclusion, myosin proteins knock down proposes a promising therapeutic strategy to combat toxoplasmosis.PMID:37331576 | DOI:10.1016/j.exppara.2023.108565
Source: Experimental Parasitology - June 18, 2023 Category: Parasitology Authors: Tayebeh Zohrabi Mojtaba Azimi-Resketi Fereshteh Talaei Maryam Yaghoubi Mohamad Reza Ganjalikhany Farzaneh Mohamadi Farsani Abbasali Eskandarian Source Type: research

Preassembled Single-Stranded RNA-Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium
Cryptosporidiosis is a common cause of diarrhea morbidity and mortality worldwide. Research progress on this infection has been slowed by lack of methods to genetically manipulate Cryptosporidium parasites. Small interfering RNA (siRNA) is widely used to study gene function, but Cryptosporidium species lack the enzymes necessary to process siRNA. By preassembling complexes with the human enzyme Argonaute 2 (hAgo2) and Cryptosporidium single-stranded RNA (ssRNA), we induced specific slicing in Cryptosporidium RNA targets. We demonstrated the reduction in expression of target genes at the mRNA and protein levels by transfect...
Source: The Journal of Infectious Diseases - March 19, 2016 Category: Infectious Diseases Authors: Castellanos-Gonzalez, A., Perry, N., Nava, S., White, A. C. Tags: PARASITES Source Type: research