CD27 expression of T-cells discriminates IGRA-negative TB patients from healthy contacts in Ghana
Publication date: Available online 12 August 2019Source: Microbes and InfectionAuthor(s): Ernest Adankwah, Alptekin Güler, Ertan Mayatepek, Richard Odame Phillips, Norman Nausch, Marc JacobsenAbstractIFN-γ release assays (IGRAs) have suboptimal sensitivity for detection of Mycobacterium tuberculosis (Mtb) infection and cannot discriminate between tuberculosis (TB) patients and healthy -potentially Mtb infected-contacts (HCs). In a case-control study we determined T-cell phenotypes of IGRAs in TB patients (n=20) and HCs (n=20) from Ghana. CD27 expression of T-cells was significantly lower in TB patients as compar...
Source: Microbes and Infection - August 14, 2019 Category: Infectious Diseases Source Type: research

Symposium report: One Health meets sequencing
Publication date: Available online 8 August 2019Source: Microbes and InfectionAuthor(s): Adrian Egli, Daniel Koch, Jürg Danuser, Rene S. Hendriksen, Susanne Driesen, Diana Coman Schmid, Richard Neher, Mirjam Mäusezahl, Helena MB. Seth-Smith, Guido Bloemberg, Sarah Tschudin-Sutter, Andrea Endimiani, Vincent Perreten, Gilbert Greub, Jacques Schrenzel, Roger StephanAbstractWhole genome sequencing (WGS) has become the new gold standard for typing and characterization of pathogens. WGS enables understanding transmission chains of bacteria, exchange of mobile genetic elements, and variation in viruses with the highest ...
Source: Microbes and Infection - August 9, 2019 Category: Infectious Diseases Source Type: research

The Best of Both Worlds- Bringing together Cell Biology and Infection at the Institut Pasteur
Publication date: Available online 30 July 2019Source: Microbes and InfectionAuthor(s): Chiara Zurzolo, Jost EnningaAbstractOnly a profound understanding of the structure and function of cells - either as single units or in the context of tissues and whole organisms - will allow a comprehension of what happens in pathological conditions and provides the means to fight disease. The Cell Biology and Infection (BCI for Biologie Cellulaire et Infection) department was created in 2002 at the Institut Pasteur in Paris to develop a research program under the umbrella of cell biology, infection biology and microbiology. Its vision...
Source: Microbes and Infection - August 1, 2019 Category: Infectious Diseases Source Type: research

Levels of miR-125a-5p are altered in Mycobacterium avium-infected macrophages and associate with the triggering of an autophagic response
Publication date: Available online 23 July 2019Source: Microbes and InfectionAuthor(s): Yang Wang, Cai Chen, Xiao-dan Xu, Hui Li, Ming-hua Chen, Jing Liu, Li-jun TangAbstractMacrophages are major pathogen-killing cells. Mycobacteria can represent a serious threat to human health, in particular Mycobacterium tuberculosis and, less so, the opportunistic Mycobacterium avium. They can cause disseminated infections because of their capacity to survive and proliferate within macrophage phagolysosomes. Accumulating evidence indicates that the regulation of miRNA expression is implicated in the mechanisms of defense of macrophages...
Source: Microbes and Infection - July 24, 2019 Category: Infectious Diseases Source Type: research

Celebrating 130 years of achievement by the Institut Pasteur
Publication date: Available online 22 July 2019Source: Microbes and InfectionAuthor(s): Stewart T. Cole (Source: Microbes and Infection)
Source: Microbes and Infection - July 23, 2019 Category: Infectious Diseases Source Type: research

Calpain regulates CVB3 induced viral myocarditis by promoting autophagic flux upon infection
In this study, we found that calpain was significantly activated in hearts affected by VMC. However, pharmacologically inhibiting calpain aggravated VMC symptoms in mice due to myocardial inflammation and cardiac dysfunction. The inhibition of calpain activity in vitro led to the accumulation of LC3-II and increased levels of p62/SQSTM1 protein expression, suggesting that autophagic flux was impaired by calpain inhibition. These effects of calpain inhibition were also observed in capn4-specific myocardial knockout mice in vivo. Furthermore, our results provided evidence that calpain inhibition in VMC, unlike other cardiova...
Source: Microbes and Infection - July 17, 2019 Category: Infectious Diseases Source Type: research

The remarkable history of the hepatitis C virus
Publication date: Available online 8 July 2019Source: Microbes and InfectionAuthor(s): Stanislas Pol, Sylvie LagayeSummaryThe infection with the hepatitis C virus (HCV) is an example of the translational research success. The reciprocal interactions between clinicians and scientists have allowed in 30 years the initiation of empirical treatments by interferon, the discovery of the virus, the development of serological and virological tools for diagnosis but also for prognosis (the non-invasive biochemical or morphological fibrosis tests, the predictors of the specific immune response including genetic IL28B polymorphisms)....
Source: Microbes and Infection - July 10, 2019 Category: Infectious Diseases Source Type: research

From septicemia to sepsis 3.0 – from Ignaz Semmelweis to Louis Pasteur
Publication date: Available online 27 June 2019Source: Microbes and InfectionAuthor(s): Jean-Marc Cavaillon, Fabrice ChrétienAbstractSepsis remains a contemporary threat, and its frequency remains high amongst an aging population. Its definition has been regularly revisited, but the impact of the translational research studying it remains very modest compared to the results seen after the introduction of hygiene and the use of antibiotics. In the past, the main forms of sepsis were hospital gangrene (also known as nosocomial fever or putrid fever) that affected the wounded, and puerperal fever that affected women sh...
Source: Microbes and Infection - June 28, 2019 Category: Infectious Diseases Source Type: research

Studying fungal pathogens of humans and fungal infections: fungal diversity and diversity of approaches
Publication date: Available online 27 June 2019Source: Microbes and InfectionAuthor(s): Guilhem Janbon, Jessica Quintin, Fanny Lanternier, Christophe d’EnfertAbstractSeminal work by Louis Pasteur revealed the contribution of fungi – yeasts and microsporidia to agroindustry and disease in animals, respectively. More than 150 years later, the impact of fungi on human health and beyond is an ever-increasing issue, although often underestimated. Recent studies estimate that fungal infections, especially those caused by Candida, Cryptococcus and Aspergillus species, kill more than one million people annually. Indeed...
Source: Microbes and Infection - June 28, 2019 Category: Infectious Diseases Source Type: research

Duclaux, Chamberland, Roux, Grancher, and Metchnikoff: the five musketeers of Louis Pasteur
Publication date: Available online 28 June 2019Source: Microbes and InfectionAuthor(s): Jean-Marc Cavaillon, Sandra LegoutAbstractThe Institut Pasteur was created thanks to worldwide generosity with the aim to welcome and treat rabies patients, to provide a place for scientific research and to offer new teaching programs in microbiology. Louis Pasteur invited his main collaborators, who had accompanied him during his previous investigations at École Normale Supérieure, to join him in his new institute. They contributed to the principle discoveries of Pasteur, such as the fight against spontaneous generation, ...
Source: Microbes and Infection - June 28, 2019 Category: Infectious Diseases Source Type: research

Novel mechanistic insights into physiological signaling pathways mediated by mycobacterial Ser/Thr protein kinases
Publication date: Available online 26 June 2019Source: Microbes and InfectionAuthor(s): Marco Bellinzoni, Anne Marie Wehenkel, Rosario Durán, Pedro M. AlzariAbstractProtein phosphorylation is known to be one of the keystones of signal sensing and transduction in all living organisms. Once thought to be essentially confined to the eukaryotic kingdoms, reversible phosphorylation on serine, threonine and tyrosine residues, has now been shown to play a major role in many prokaryotes, where the number of Ser/Thr protein kinases (STPKs) equals or even exceeds that of two component systems. Mycobacterium tuberculosis, the ...
Source: Microbes and Infection - June 28, 2019 Category: Infectious Diseases Source Type: research

The IL-23/IL-17 pathway in human chronic inflammatory diseases – new insight from genetics and targeted therapies
Publication date: Available online 25 June 2019Source: Microbes and InfectionAuthor(s): Elisabetta Bianchi, Lars RoggeAbstractChronic inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease, spondyloarthritis and psoriasis cause significant morbidity and are a considerable burden for the patients in terms of pain, impaired function and diminished quality of life, as well as for society, because of the associated high health-care costs, and loss of productivity. Our limited understanding of the pathogenic mechanisms involved in these diseases currently hinders early diagnosis and the development of mo...
Source: Microbes and Infection - June 26, 2019 Category: Infectious Diseases Source Type: research

Intracellular parasitism, the driving force of evolution of Legionella pneumophila and the genus Legionella
Publication date: Available online 25 June 2019Source: Microbes and InfectionAuthor(s): Laure Gomez-Valero, Carmen BuchrieserAbstractLegionella pneumophila is an intracellular pathogen that causes a severe pneumonia called Legionnaires’ disease that is often fatal when not promptly diagnosed and treated. Legionella parasitize aquatic protozoa with which it co-evolved over an evolutionary long time. The close relationship between hosts and pathogens, their co-evolution, led to molecular interactions such as the exchange of genetic material through horizontal gene transfer (HGT). Genome sequencing of L. pneumophila and...
Source: Microbes and Infection - June 26, 2019 Category: Infectious Diseases Source Type: research

Trypanosoma cruzi extracellular amastigotes selectively trigger the PI3K/Akt and Erk pathways during HeLa cell invasion
Publication date: Available online 24 June 2019Source: Microbes and InfectionAuthor(s): Bianca Lima Ferreira, Éden Ramalho Ferreira, Alexis Bonfim-Melo, Renato Arruda Mortara, Diana BahiaAbstractCell invasion by Trypanosoma cruzi extracellular amastigotes involves different signaling pathways to induce phagocytosis-like mechanisms. Previous works indicated that PI3K/Akt, Src and Erk might be involved in EA invasion; however, participation of these molecules in this process remains elusive. Here, we observed that EA activated Akt, Erk but not Src. Interference of EA invasion with specific inhibitors corroborated this...
Source: Microbes and Infection - June 25, 2019 Category: Infectious Diseases Source Type: research

Yersinia pestis and plague: an updated view on evolution, virulence determinants, immune subversion, vaccination and diagnostics
Publication date: Available online 25 June 2019Source: Microbes and InfectionAuthor(s): Christian Demeure, Olivier Dussurget, Guillem Mas Fiol, Anne-Sophie Le Guern, Cyril Savin, Javier Pizarro-CerdáAbstractPlague is a vector-borne disease caused by Yersinia pestis. Transmitted by fleas from rodent reservoirs, Y. pestis emerged less than 6000 years ago from an enteric bacterial ancestor through events of gene gain and genome reduction. It is a highly remarkable model for the understanding of pathogenic bacteria evolution, and a major concern for public health as highlighted by recent human outbreaks. A complex set o...
Source: Microbes and Infection - June 25, 2019 Category: Infectious Diseases Source Type: research

130th Anniversary of Institut Pasteur: Celebrating Science
Publication date: Available online 25 June 2019Source: Microbes and InfectionAuthor(s): Marie-Lise Gougeon, Maxime Schwartz, Thomas Brunner, David M. Ojcius (Source: Microbes and Infection)
Source: Microbes and Infection - June 25, 2019 Category: Infectious Diseases Source Type: research

Beneficial microbiota. Probiotics and pharmaceutical products in functional nutrition and medicine
Publication date: Available online 21 June 2019Source: Microbes and InfectionAuthor(s): Galina Novik, Victoria SavichAbstractThe article is mainly devoted to such representatives of gut microbiota as lactic acid bacteria and bifidobacteria, with minor accent on less frequently used or new probiotic microorganisms. Positive effects in treatment and prevention of diseases by different microbial groups, their metabolites and mechanisms of action, management and market of probiotic products are considered. (Source: Microbes and Infection)
Source: Microbes and Infection - June 22, 2019 Category: Infectious Diseases Source Type: research

Apathetic Pathogens
Publication date: Available online 18 June 2019Source: Microbes and InfectionAuthor(s): Sophia Häfner (Source: Microbes and Infection)
Source: Microbes and Infection - June 19, 2019 Category: Infectious Diseases Source Type: research

Host poly(ADP-ribose) polymerases (PARPs) in acute and chronic bacterial infections
Publication date: Available online 15 June 2019Source: Microbes and InfectionAuthor(s): Moona Miettinen, Madhukar Vedantham, Arto T. PulliainenAbstractProtein ADP-ribosylation is a reversible post-translational modification, which alters protein activity, localization, interactome or stability, leading to perturbation of cell signaling. This review summarizes the emerging data indicating that host cell ADP-ribosylating enzymes, poly(ADP-ribose) polymerases (PARPs), influence the course of a bacterial infection, in parallel to ADP-ribosylating bacterial toxins. Host cell PARP targeting could be an efficient therapeutic appr...
Source: Microbes and Infection - June 16, 2019 Category: Infectious Diseases Source Type: research

Prophylactic and therapeutic vaccines against sporotrichosis. Feasibility and prospects
Publication date: Available online 13 June 2019Source: Microbes and InfectionAuthor(s): Damiana Téllez-Martínez, Alexander Batista-Duharte, Deivys Leandro Portuondo, Iracilda Zeppone CarlosAbstractSporotrichosis is an emergent subcutaneous mycosis of humans and some animals caused by dimorphic fungi of the genus Sporothrix. The disease occurs worldwide but is endemic or hyperendemic in tropical and subtropical areas. The epidemiology of the disease is changing dramatically, and it is now considered an important zoonosis with high morbidity rates, principally in Brazil, and an opportunistic infection in immuno...
Source: Microbes and Infection - June 13, 2019 Category: Infectious Diseases Source Type: research

Autophagic Induction modulates Splenic Plasmacytoid Dendritic Cell mediated immune response in Cerebral Malarial infection model
Publication date: Available online 8 June 2019Source: Microbes and InfectionAuthor(s): Anirban Sengupta, Tarun Keswani, Samrat Sarkar, Soubhik Ghosh, Saikat Mukherjee, Arindam BhattacharyyaAbstractSplenic plasmacytoid dendritic cells (pDC) possess the capability to harbor live replicative Plasmodium parasite. Isolated splenic pDC from infected mice causes malaria when transferred to naïve mice. Incomplete autophagic degradation might cause poor antigen processing and poor immune response. Induction of autophagic flux by rapamycin treatment led to better prognosis by boosting pDC centered immune response against the pa...
Source: Microbes and Infection - June 8, 2019 Category: Infectious Diseases Source Type: research

Direct correlation between Th1 and Th17 responses in immunity to Brucella infection
This study aimed to evaluate the peripheral distributions of Th1 and Th17 cells and serum levels of IFN-γ, IL-17A and IL-22 cytokines in brucellosis patients. One hundred patients (36 acute, 41 under-treatment and 23 relapsed) and 30 age- and sex-matched healthy controls were included. The frequencies of Th1 and Th17 cells were determined by flow cytometric analysis. Serum levels of IFN-γ, IL-17A and IL-22 were measured by multi-analyte flow assay. Increased frequencies of Th1 and Th17 cells were observed in acute and relapsed brucellosis versus under-treatment patients and healthy controls (P
Source: Microbes and Infection - June 8, 2019 Category: Infectious Diseases Source Type: research

Zika Virus Infection: An Update
Publication date: Available online 31 May 2019Source: Microbes and InfectionAuthor(s): Pauline Ferraris, Hans Yssel, Dorothée MisséAbstractSince the ZIKV outbreak in Brazil in 2015, the scientific community has joined efforts to gather more information on the epidemiology, clinical features and pathogenicity of the virus. Here, we summarize the most important advances made recently and discuss promising, innovative aproaches to understand and control ZIKV infection. (Source: Microbes and Infection)
Source: Microbes and Infection - June 1, 2019 Category: Infectious Diseases Source Type: research

Helicobacter pylori pediatric infection changes FcεRI expression in dendritic cells and Treg profile in vivo and in vitro
Publication date: Available online 22 May 2019Source: Microbes and InfectionAuthor(s): Miguel A. Leon, Camila Palma, Caroll Hernandez, Mauricio Sandoval, Colomba Cofre, Guillermo Perez-Mateluna, Arturo Borzutzky, Paul R. Harris, Carolina A. SerranoAbstractH. pylori infection shows an inverse relationship with allergies. Dendritic cells regulate mucosal immune responses including the induction of T regulatory cells which are fundamental in H. pylori-induced dampening of allergies. In this respect expression of high-affinity IgE receptor (FcεRI) has been associated with a regulatory dendritic cell profile. Therefore ...
Source: Microbes and Infection - May 23, 2019 Category: Infectious Diseases Source Type: research

ESCMID/ESGMD postgraduate technical workshop on diagnostic microbiology
Publication date: Available online 16 May 2019Source: Microbes and InfectionAuthor(s): Aurélie Scherler, Silvia Ardissone, Jacob Moran-Gilad, Gilbert GreubAbstractThe European Society of Clinical Microbiology and Infectious Diseases (ESCMID) workshop on diagnostic microbiology was held in Lausanne from 25th to 28th September 2018. A total of 87 participants from 31 countries participated to this postgraduate technical workshop. Over 4 days, the mornings were dedicated to talks, which covered many subjects including MALDI-TOF, molecular diagnosis, genomics and metagenomics as well as new innovative approaches. The af...
Source: Microbes and Infection - May 16, 2019 Category: Infectious Diseases Source Type: research

Role of Escherichia coli endopeptidases and DD-carboxypeptidases in infection and regulation of innate immune response
Publication date: Available online 11 May 2019Source: Microbes and InfectionAuthor(s): Sathi Mallick, Joyjyoti Das, Jyoti Verma, Samatha Mathew, Tapas K. Maiti, Anindya S. GhoshAbstractThe low-molecular-mass penicillin-binding proteins, involved in peptidoglycan recycling can also produce peptidoglycan fragments capable of activating an innate immune response in host. To investigate how these proteins in Enterobacteriaceae play a role to elicit/evade innate immune responses during infections, we deleted certain endopeptidases and DD-carboxypeptidases from E. coli CS109 and studied the viability of these mutants in macropha...
Source: Microbes and Infection - May 12, 2019 Category: Infectious Diseases Source Type: research

The influence of pH on S. saprophyticus iron metabolism and the production of siderophores
Publication date: Available online 7 May 2019Source: Microbes and InfectionAuthor(s): Bianca Silva Vieira de Souza, Karla Christina Sousa Silva, Ana Flávia Alves Parente, Clayton Luiz Borges, Juliano Domiraci Paccez, Maristela Pereira, Célia Maria de Almeida Soares, Marcia Giambiagi-deMarval, Mirelle Garcia Silva Bailão, Juliana Alves Parente-RochaAbstractStaphylococcus saprophyticus is a gram-positive coagulase negative bacteria which shows clinical importance due to its capability of causing urinary tract infections (UTI), as well as its ability to persist in this environment. Little is known about h...
Source: Microbes and Infection - May 8, 2019 Category: Infectious Diseases Source Type: research

An Imbalance of T Cell Subgroups Exists in Children with Sepsis
Publication date: Available online 19 April 2019Source: Microbes and InfectionAuthor(s): Qing Ye, Wen-xia Shao, Qing-qing Wang, Jian-hua MaoAbstractThe purpose of this study is to explore the role of different T cell subgroups in the pathogenesis of sepsis in children. Flow cytometry was used to detect the changes in the activation status and the number of T cell subgroups in the peripheral blood of children with sepsis; healthy children were selected as the control group. Compared with healthy children, the number of CD4+ T cells in the peripheral blood of children with sepsis did not change significantly (Z = 1.945, P = ...
Source: Microbes and Infection - April 20, 2019 Category: Infectious Diseases Source Type: research

Characterizing the role of tissue-type plasminogen activator in a mouse model of Group A streptococcal infection
This study suggests that tPA has a limited role in this humanized model of GAS infection, further highlighting the importance of its counterpart uPA in GAS disease. (Source: Microbes and Infection)
Source: Microbes and Infection - April 20, 2019 Category: Infectious Diseases Source Type: research

VraSR has an important role in immune evasion of Staphylococcus aureus with low level vancomycin resistance
Publication date: Available online 19 April 2019Source: Microbes and InfectionAuthor(s): Caihong Gao, Yuanyuan Dai, Wenjiao Chang, Chao Fang, Ziran Wang, Xiaoling MaAbstractVancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) are increasingly being reported as associated with treatment failure. Previous studies indicated that VISA/hVISA resists clearance by the host immune system, thereby allowing persistence within the host. VraSR is a vancomycin-resistance-associated sensor/regulator that is highly expressed in VISA/hVISA strains. Whether VraSR plays an important role in immune escape by VI...
Source: Microbes and Infection - April 20, 2019 Category: Infectious Diseases Source Type: research

Cholera in the time of Likes Inspirational Pathogens and an Important Pump
Publication date: Available online 17 April 2019Source: Microbes and InfectionAuthor(s): Sophia Häfner (Source: Microbes and Infection)
Source: Microbes and Infection - April 18, 2019 Category: Infectious Diseases Source Type: research

Identification and characterization of Paracoccidioides lutzii proteins interacting with macrophages
Publication date: Available online 2 April 2019Source: Microbes and InfectionAuthor(s): Mariana Vieira Tomazett, Lílian Cristiane Baeza, Juliano Domiraci Paccez, Juliana Alves Parente-Rocha, Fátima Ribeiro-Dias, Célia Maria de Almeida SoaresAbstractParacoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a systemic disorder that involves the lungs and other organs. The adherence of pathogenic microorganisms to host tissues is an essential event in the onset of colonization and spread. The host-pathogen interaction is a complex interplay between the defense mechani...
Source: Microbes and Infection - April 3, 2019 Category: Infectious Diseases Source Type: research

IL-17A and IL-17F repair HIV-1 gp140 damaged Caco-2 cell barriers by upregulating tight junction genes
Publication date: Available online 2 April 2019Source: Microbes and InfectionAuthor(s): Fengjie Wang, Yanfang Cui, Xiuli Shen, Shuhui Wang, Gui-bo YangAbstractIt is widely accepted that impairment of the intestinal epithelial barrier from HIV/AIDS contributes significantly to microbial translocation and systemic immune activation. Such factors present potential targets for novel treatments aimed toward a functional cure. However, the extracellular mechanisms of intestinal barrier repair are poorly understood. In the current study, we investigated the abilities of IL-17A and IL-17F to repair the damaged barrier caused by HI...
Source: Microbes and Infection - April 3, 2019 Category: Infectious Diseases Source Type: research

Editorial board
Publication date: March 2019Source: Microbes and Infection, Volume 21, Issue 2Author(s): (Source: Microbes and Infection)
Source: Microbes and Infection - March 31, 2019 Category: Infectious Diseases Source Type: research

Culture-attenuated pathogenic Leptospira lose the ability to survive to Complement-mediated-killing due to lower expression of Factor H binding proteins
Publication date: Available online 25 March 2019Source: Microbes and InfectionAuthor(s): Azaf Moreno-Torres, Irving R. Malvido-Jiménez, Alejandro de la Peña-Moctezuma, Luz O. Castillo Sánchez, Tatiana R. Fraga, Angela S. Barbosa, Lourdes Isaac, Alfredo Sahagún-RuizAbstractSeveral pathogens including Gram-negative bacteria hijack complement regulators to escape host’s innate response. Pathogenic Leptospira species bind Factor H, C4b binding protein and vitronectin from the complement system. We evaluated the ability of low passage (LP) and culture-attenuated (CA) pathogenic strains of Lepto...
Source: Microbes and Infection - March 26, 2019 Category: Infectious Diseases Source Type: research

Studies on formulation of a combination heat killed immunogen from diarrheagenic Escherichia coli and Vibrio cholerae in RITARD model
Publication date: Available online 7 March 2019Source: Microbes and InfectionAuthor(s): Priyadarshini Mukherjee, Vivek Mondal, Ushasi Bhaumik, Ritam Sinha, Sounak Sarkar, Soma Mitra, Debaki Ranjan Howlader, Suhrid Maiti, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta KoleyAbstractMultiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we formulated a combination of antigens from three different diarrheagenic Escherichia coli strains and three different Vibrio cholerae strains. We demonstra...
Source: Microbes and Infection - March 9, 2019 Category: Infectious Diseases Source Type: research

Enterovirus D68 and Acute Flaccid Myelitis: Do We Need a Measles Mentality?
Publication date: Available online 4 March 2019Source: Microbes and InfectionAuthor(s): Jason A. Tetro (Source: Microbes and Infection)
Source: Microbes and Infection - March 6, 2019 Category: Infectious Diseases Source Type: research

Messages from the third International Conference on Clinical Metagenomics (ICCMg3)
Publication date: Available online 2 March 2019Source: Microbes and InfectionAuthor(s): Etienne Ruppé, Jacques SchrenzelAbstractClinical metagenomics (CMg), referring to as the application of metagenomic sequencing of clinical samples in order to recover clinically-relevant information, has been rapidly evolving these last years. Following this trend, we held the third International Conference on Clinical Metagenomics (ICCMg3) in Geneva in October 2018. During the two days of the conference, several aspects of CMg were addressed, which we propose to summarize in the present manuscript. During this ICCMg3, we kept on...
Source: Microbes and Infection - March 4, 2019 Category: Infectious Diseases Source Type: research

Advances in diagnostics, vaccines and therapeutics for Nipah virus
Publication date: Available online 26 February 2019Source: Microbes and InfectionAuthor(s): Nazia Thakur, Dalan BaileyAbstractNipah virus is an emerging zoonotic paramyxovirus that causes severe and often fatal respiratory and neurological disease in humans. The virus was first discovered after an outbreak of encephalitis in pig farmers in Malaysia and Singapore with subsequent outbreaks in Bangladesh or India occurring almost annually. Due to the highly pathogenic nature of NiV, its pandemic potential, and the lack of licensed vaccines or therapeutics, there is a requirement for research and development into highly sensit...
Source: Microbes and Infection - February 26, 2019 Category: Infectious Diseases Source Type: research

Pam3CSK4 adjuvant given intranasally boosts anti-Leishmania immunogenicity but not protective immune responses conferred by LaAg vaccine against visceral leishmaniasis
Publication date: Available online 25 February 2019Source: Microbes and InfectionAuthor(s): Caio Loureiro Salgado, Emmanoel Loss Dias, Lorenzzo Lyrio Stringari, Luciana Polaco Covre, Reynaldo Dietze, Fausto Edimundo Lima Pereira, Herbet Leonel De Mattos Guedes, Bartira Rossi-Bergmann, Daniel Claudio Oliveira GomesAbstractThe use of adjuvants in vaccine formulations is a well-established practice to improve immunogenicity and protective immunity against diseases. Previously, we have demonstrated the feasibility of intranasal vaccination with the antigen of killed Leishmania amazonensis promastigotes (LaAg) against experimen...
Source: Microbes and Infection - February 25, 2019 Category: Infectious Diseases Source Type: research

Protective potency of recombinant meningococcal IgA1 protease and its structural derivatives upon animal invasion with meningococcal and pneumococcal infections
Publication date: Available online 22 February 2019Source: Microbes and InfectionAuthor(s): Olga Kotelnikova, Alexander Alliluev, Alexei Zinchenko, Larisa Zhigis, Yuri Prokopenko, Elena Nokel, Olga Razgulyaeva, Vera Zueva, Marina Tokarskaya, Natalia Yastrebova, Elena Gordeeva, Tatyana Melikhova, Elena Kaliberda, Lev RumshAbstractImmunization of mice with recombinant IgA1 protease of Neisseria meningitidis or several structural derivatives thereof protects the animals infected with a variety of deadly pathogens, including N. meningitidis serogroups A, B, and C and 3 serotypes of Streptococcus pneumonia. In sera of rabbits i...
Source: Microbes and Infection - February 22, 2019 Category: Infectious Diseases Source Type: research

Editorial board
Publication date: January–February 2019Source: Microbes and Infection, Volume 21, Issue 1Author(s): (Source: Microbes and Infection)
Source: Microbes and Infection - February 15, 2019 Category: Infectious Diseases Source Type: research

Colonic adenoma-associated Escherichia coli express specific phenotypes
This study was aimed at characterizing isolates collected from polyps and adjacent tissue in comparison with those from normal mucosa.From colonoscopy biopsies, 1500 E. coli isolates were retrieved and genotyped; 272 were characterized for phylogroup and major phenotypic traits (i.e., biofilm formation, motility, hemolysins, and proteases). Selected isolates were analyzed for extraintestinal pathogenic E. coli (ExPEC)-associated virulence genes and in vivo pathogenicity using Galleria mellonella.The majority of isolates collected from polyps were strong biofilm and poor protease producers, whereas those isolates from norma...
Source: Microbes and Infection - February 12, 2019 Category: Infectious Diseases Source Type: research

Porphyromonas gingivalis promotes the motility of esophageal squamous cell carcinoma by activating NF-κB signaling pathway
Publication date: Available online 11 February 2019Source: Microbes and InfectionAuthor(s): Fan Meng, Ruifeng Li, Liyu Ma, Lifang Liu, Xiaorong Lai, Dongyang Yang, Junmin Wei, Dong Ma, Zijun LiAbstractEsophageal carcinoma, with a increasing incidence, is one of the most aggressive carcinomas in gastrointestinal tract. Epidemiologic studies demonstrate an association of oral pathogens with multiple diseases, including rheumatoid arthritis, cardiovascular diseases, diabetes, and gastrointestinal malignancies. Nevertheless, a causal relationship between oral pathogens and esophageal squamous cell carcinoma (ESCC) has not been...
Source: Microbes and Infection - February 12, 2019 Category: Infectious Diseases Source Type: research

Dentin matrix protein 1 correlates with the severity of hemorrhagic fever with renal syndrome and promotes hyper-permeability of endothelial cells infected by Hantaan virus
This study suggests that elevated DMP1 concentrations may be associated with disease stage, severity, and the degree of acute kidney injury. DMP1 is involved in the regulation of capillary permeability in HFRS caused by hantavirus infection. (Source: Microbes and Infection)
Source: Microbes and Infection - February 6, 2019 Category: Infectious Diseases Source Type: research

Endocrine modulation of Brucella abortus-infected osteocytes function and osteoclastogenesis via modulation of RANKL/OPG
Publication date: Available online 5 February 2019Source: Microbes and InfectionAuthor(s): Ayelén Ivana Pesce Viglietti, Guillermo Hernán Giambartolomei, María Victoria DelpinoAbstractOsteoarticular brucellosis is the most frequent complication of active disease. A large amount of cells in bone are osteocytes. Since bone remodeling process is regulated by hormones we sought to study the effect of cortisol and DHEA in B. abortus-infected osteocytes. Cortisol treatment inhibited the expression of IL-6, TNF-α, MMP-2 and RANKL in B. abortus-infected osteocytes. DHEA could reverse the inhibitory effec...
Source: Microbes and Infection - February 6, 2019 Category: Infectious Diseases Source Type: research

Asymptomatic dengue virus cases in misiones, Argentina: a seroprevalence study in the university population
In this study, 288 samples were analyzed, and the IgG anti dengue antibodies detected accounted for 6.6% of cases, while 89% corresponded to individuals who lived with people diagnosed or suspected of having contracted dengue, p=
Source: Microbes and Infection - February 5, 2019 Category: Infectious Diseases Source Type: research

Guns N′ viruses
Publication date: Available online 23 January 2019Source: Microbes and InfectionAuthor(s): Sophia Häfner (Source: Microbes and Infection)
Source: Microbes and Infection - February 5, 2019 Category: Infectious Diseases Source Type: research

Guns N' Viruses
Publication date: Available online 23 January 2019Source: Microbes and InfectionAuthor(s): Sophia Häfner (Source: Microbes and Infection)
Source: Microbes and Infection - January 24, 2019 Category: Infectious Diseases Source Type: research

Transcriptional profiles of cytokines and chemokines reveal important pro-inflammatory response from endothelial cells during Orientia tsutsugamushi infection
Publication date: Available online 23 January 2019Source: Microbes and InfectionAuthor(s): Hong Ge, Christina M. Farris, Min Tong, Alice Maina, Allen L. RichardsAbstractEndothelial cells (EC) are key targets during Orientia tsutsugamushi infection. Knowledge of the pro-inflammatory response against O. tsutsugamushi by ECs is limited. The aim of the present study was to characterize the pro-inflammatory transcriptional response during the first 24 hours of infection of the human dermal microvascular endothelial cell line with O. tsutsugamushi Karp by examining five-time points. The transcriptional profiles of 84 genes inclu...
Source: Microbes and Infection - January 24, 2019 Category: Infectious Diseases Source Type: research