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Source: Am J Physiol Cell Ph...

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Total 155 results found since Jan 2013.

Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via Wnt/ β-catenin signaling pathway.
Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via Wnt/β-catenin signaling pathway. Am J Physiol Cell Physiol. 2018 Aug 01;: Authors: Gao D, Chen HQ, Yang YM Abstract Metastatic cutaneous squamous cell carcinoma (CSCC) is a major cause of death associated with non-melanoma skin cancer. The involvement of homeobox B7 (HOXB7) in cancers has been reported. Thus, the current study intends to explore the effect of HOXB7 on CSCC and its relationship with the Wnt/β-catenin signaling pathway. Initially, microarray-based gene expres...
Source: Am J Physiol Cell Ph... - August 1, 2018 Category: Cytology Authors: Gao D, Chen HQ, Yang YM Tags: Am J Physiol Cell Physiol Source Type: research

Adenosinergic Signaling Inhibits Oxalate Transport by Human Intestinal Caco2-BBE Cells Through the A2B Adenosine Receptor.
Abstract Most kidney stones (KS) are composed of calcium oxalate, and small increases in urine oxalate affect the stone risk. Intestinal oxalate secretion mediated by anion exchanger SLC26A6 (PAT1) plays a crucial role in limiting net absorption of ingested oxalate; thereby preventing hyperoxaluria and related KS, reflecting the importance of understanding regulation of intestinal oxalate transport. We previously showed that ATP and UTP inhibit oxalate transport by human intestinal Caco2-BBE cells (C2). Since ATP is rapidly degraded to adenosine (ADO), we examined whether intestinal oxalate transport is regulated ...
Source: Am J Physiol Cell Ph... - July 18, 2018 Category: Cytology Authors: Jung D, Alshaikh A, Ratakonda S, Bashir M, Amin MR, Jeon S, Stevens J, Sharma S, Ahmed W, Musch M, Hassan H Tags: Am J Physiol Cell Physiol Source Type: research

A2BAR activation attenuates acute lung injury by inhibiting alveolar epithelial cell apoptosis both in vivo and in vitro.
In conclusion, A2BAR activation effectively attenuated OA-induced ALI by inhibiting AEC apoptosis and mitigated H2O2-induced AEC injury by suppressing the p38 and ERK1/2-mediated mitochondrial apoptosis pathway. PMID: 29898376 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - June 13, 2018 Category: Cytology Authors: Xu X, Zhu Q, Niu F, Zhang R, Wang Y, Wang W, Sun D, Wang X, Wang A Tags: Am J Physiol Cell Physiol Source Type: research

The role of microRNA-1 targeting MAPK3 in myocardial ischemia/reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway.
Abstract Recent studies have uncovered the vital roles played by microRNAs (miRNAs) in regulating cardiac injury. Amongst them, the cardiac enriched microRNA-1 (miR-1) has been extensively studied and proven to be detrimental to cardiac myocytes. Hence, the current study aims to explore whether miR-1 affects myocardial ischemia/reperfusion injury (MIRI) in rats undergoing sevoflurane preconditioning and the underlying mechanism. After successful model establishment, rats with MIRI were transfected with mimics or inhibitors of miR-1, or siRNA against MAPK3, and then were injected with sevoflurane. A luciferase repo...
Source: Am J Physiol Cell Ph... - May 9, 2018 Category: Cytology Authors: Hao YL, Fang HC, Zhao HL, Li XL, Luo Y, Wu BQ, Fu MJ, Liu W, Liang JJ, Chen XH Tags: Am J Physiol Cell Physiol Source Type: research

Reactive oxygen species up-regulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells.
K, Sokabe M, Nikawa T Abstract Unloading-mediated muscle atrophy is associated with increased reactive oxygen species (ROS) production. We previously demonstrated that elevated ubiquitin ligase casitas B-lineage lymphoma-b (Cbl-b) resulted in the loss of muscle volume [Nakao R. et al. Mol Cell Biol, 29: 4798-4811, 2009]. However, the pathological role of ROS production associated with unloading-mediated muscle atrophy still remains unknown. Here, we showed the ROS-mediated signal transduction caused by microgravity or its simulation contributes to Cbl-b expression. In L6 myotubes, the assessment of redox status re...
Source: Am J Physiol Cell Ph... - March 7, 2018 Category: Cytology Authors: Uchida T, Sakashita Y, Kitahata K, Yamashita Y, Tomida C, Kimori Y, Komatsu A, Hirasaka K, Ohno A, Nakao R, Higashitani A, Higashibata A, Ishioka N, Shimazu T, Kobayashi T, Okumura Y, Choi I, Oarada M, Mills EM, Teshima-Kondo S, Takeda S, Tanaka E, Tanaka Tags: Am J Physiol Cell Physiol Source Type: research

Mitochondrial Inner Membrane Protein (Mitofilin) Knockdown Induces Cell Death by Apoptosis Via an AIF-PARP-Dependent Mechanism and Cell Cycle Arrest.
Abstract Mitofilin is an inner membrane protein that has been defined as a mitochondria-shaping protein in controlling and maintaining mitochondrial cristae structure and remodeling. We determined the role of mitofilin in cell survival by investigating the mechanism underlying mitofilin knockdown-induced cell death by apoptosis. Cultured H9c2 myoblasts and HEK 293 cells were treated with mitofilin siRNA or scrambled siRNA for 24 hours. Cell death (apoptosis), caspase 3 activity, and cell cycle phases were assessed by flow cytometry, while cytochrome C release and intracellular ATP production were measured by ELISA...
Source: Am J Physiol Cell Ph... - February 28, 2018 Category: Cytology Authors: Madungwe NB, Feng Y, Lie M, Tombo N, Liu L, Kaya F, Bopassa JC Tags: Am J Physiol Cell Physiol Source Type: research

Neonatal cardiomyocyte hypertrophy induced by endothelin-1 is blocked by estradiol acting on GPER.
In conclusion, E2 plays a key role in antagonizing ET-1-induced hypertrophy in cultured neonatal cardiomyocytes through GPER signaling by mechanism involving activation of PDK1 pathway, which would prevent the increase of ERK1/2 activity and consequently the development of hypertrophy. PMID: 29167148 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - November 22, 2017 Category: Cytology Authors: Goncalves GK, Scalzo S, Alves AP, Agero U, Guatimosim S, Reis AM Tags: Am J Physiol Cell Physiol Source Type: research

Acute myotube protein synthesis regulation by IL-6-related cytokines.
Abstract IL-6 and leukemia inhibitory factor (LIF), members of the IL-6 family of cytokines, play recognized paradoxical roles in skeletal muscle mass regulation, being associated with both growth and atrophy. Overload or muscle contractions can induce a transient increase in muscle IL-6 and LIF expression, which has a regulatory role in muscle hypertrophy. However, the cellular mechanisms involved in this regulation have not been completely identified. The induction of mammalian target of rapamycin complex 1 (mTORC1)-dependent myofiber protein synthesis is an established regulator of muscle hypertrophy, but the i...
Source: Am J Physiol Cell Ph... - November 1, 2017 Category: Cytology Authors: Gao S, Durstine JL, Koh HJ, Carver WE, Frizzell N, Carson JA Tags: Am J Physiol Cell Physiol Source Type: research

NF- κB-Dependent Upregulation of (Pro)Renin Receptor Mediates High NaCl-Induced Apoptosis in Mouse Inner Medullary Collecting Duct Cells.
In conclusion, PRR expression is induced by high NaCl through NF-κB and contributes to cell apoptosis under this circumstance. PMID: 29021196 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - October 11, 2017 Category: Cytology Authors: Su J, Liu X, Xu C, Lu X, Wang F, Fang H, Lu A, Qiu Q, Li C, Yang T Tags: Am J Physiol Cell Physiol Source Type: research

Involvement of connexin 43 phosphorylation and gap junctional communication between smooth muscle cells in vasopressin-induced ROCK-dependent vasoconstriction after hemorrhagic shock.
In conclusion, AVP-mediated vascular contractile effects are endothelium and myoendothelial gap junction independent. Gap junctions between VSMCs, gap junctional communication, and Cx43 phosphorylation at Ser(262) play important roles in the vascular effects of AVP. RhoA/ROCK, but not PKC, is involved in this process. PMID: 28974518 [PubMed - in process]
Source: Am J Physiol Cell Ph... - October 1, 2017 Category: Cytology Authors: Yang G, Peng X, Wu Y, Li T, Liu L Tags: Am J Physiol Cell Physiol Source Type: research

(Pro) Renin Receptor Mediates Albumin Induced Cellular Responses: Role of Site-1 Protease-Derived Soluble (Pro) Renin Receptor in Renal Epithelial Cells.
Abstract Proteinuria is a characteristic of chronic kidney disease (CKD) and also a causative factor that promotes the disease progression in part via activation of intrarenal renin-angiotensin system (RAS). (Pro) renin receptor (PRR), a newly discovered component of the RAS, binds renin and (pro) renin to promote angiotensin I generation. The present study was performed to test the role of soluble PRR (sPRR) in albumin overload-induced responses in cultured human renal proximal tubular cell line HK-2 cells. Bovine serum albumin (BSA) treatment for 24 h at 20 mg/ml induced renin activity and inflammation, both of ...
Source: Am J Physiol Cell Ph... - September 13, 2017 Category: Cytology Authors: Fang H, Xu C, Lu A, Zou CJ, Xie S, Chen Y, Zhou L, Liu M, Wang L, Wang W, Yang T Tags: Am J Physiol Cell Physiol Source Type: research

Integration of TRPC6 and NADPH Oxidase Activation in Lysophosphatidylcholine-induced TRPC5 Externalization.
Abstract Lipid oxidation products, including lysophosphatidylcholine (lysoPC), activate TRPC6, and the subsequent increase in intracellular Ca(2+) leads to TRPC5 activation. The goal of this study is to elucidate the steps in the pathway between TRPC6 activation and TRPC5 externalization. Following TRPC6 activation by lysoPC, extracellular regulated kinase (ERK) is phosphorylated. This leads to phosphorylation of p47(phox) and subsequent NADPH oxidase activation with increased production of reactive oxygen species. ERK activation requires TRPC6 opening and influx of Ca(2+) as evidenced by the failure of lysoPC to ...
Source: Am J Physiol Cell Ph... - August 23, 2017 Category: Cytology Authors: Chaudhuri P, Rosenbaum MA, Birnbaumer L, Graham LM Tags: Am J Physiol Cell Physiol Source Type: research

Involvement of connexin43 phosphorylation and gap junctional communication in vasopressin-induced ROCK-dependent vasoconstriction after hemorrhagic shock.
In conclusion, AVP-mediated vascular contractile effects are endothelium and myoendothelial gap junctions independent. Gap junctions between VSMCs, gap-junctional communication and Cx43 phosphorylation at Ser262 play important roles in the vascular effects of AVP. RhoA/ROCK, but not PKC, is involved in this process. PMID: 28794110 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - August 9, 2017 Category: Cytology Authors: Yang G, Peng X, Wu Y, Li T, Liu L Tags: Am J Physiol Cell Physiol Source Type: research

Myoendothelial gap junctions mediate regulation of angiopoietin-2 induced vascular hyporeactivity after hypoxia through connexin 43-gated cAMP transfer.
Abstract Angiopoietin-2 (Ang2) contributes to vascular hyporeactivity after hemorrhagic shock and hypoxia through upregulation of inducible nitric oxide synthase (iNOS), in a vascular endothelial cell (VEC)-specific and Ang2/Tie2 receptor-dependent manner. While iNOS is primarily expressed in vascular smooth muscle cells (VSMCs), the mechanisms of signal transfer from VECs to VSMCs are unknown. A double-sided co-culture model with VECs and VSMCs from Sprague Dawley rats was used to investigate the role of myoendothelial gap junctions (MEGJs), the connexin (Cx) isoforms involved and other relevant mechanisms. After...
Source: Am J Physiol Cell Ph... - June 21, 2017 Category: Cytology Authors: Xu J, Yang GM, Li T, Liu LM Tags: Am J Physiol Cell Physiol Source Type: research

ROS-induced ROS Release Orchestrated by Nox4, Nox2 and Mitochondria in VEGF Signaling and Angiogenesis.
Abstract Reactive oxygen species (ROS) derived from NADPH oxidase (NOX) and mitochondria play a critical role in growth factor-induced switch from a quiescent to an angiogenic phenotype in endothelial cells (ECs). However, how highly diffusible ROS produced from different sources can coordinate to stimulate VEGF signaling and drive the angiogenic process remains unknown. Using the cytosol- and mitochondria-targeted redox-sensitive RoGFP biosensors with real-time imaging, here we show that VEGF stimulation in human ECs rapidly increases cytosolic RoGFP oxidation within 1 min, followed by mitochondrial RoGFP oxidati...
Source: Am J Physiol Cell Ph... - April 19, 2017 Category: Cytology Authors: Kim YM, Kim SJ, Tatsunami R, Yamamura H, Fukai T, Ushio-Fukai M Tags: Am J Physiol Cell Physiol Source Type: research