The role of microRNA-1 targeting MAPK3 in myocardial ischemia/reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway.

The role of microRNA-1 targeting MAPK3 in myocardial ischemia/reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway. Am J Physiol Cell Physiol. 2018 May 09;: Authors: Hao YL, Fang HC, Zhao HL, Li XL, Luo Y, Wu BQ, Fu MJ, Liu W, Liang JJ, Chen XH Abstract Recent studies have uncovered the vital roles played by microRNAs (miRNAs) in regulating cardiac injury. Amongst them, the cardiac enriched microRNA-1 (miR-1) has been extensively studied and proven to be detrimental to cardiac myocytes. Hence, the current study aims to explore whether miR-1 affects myocardial ischemia/reperfusion injury (MIRI) in rats undergoing sevoflurane preconditioning and the underlying mechanism. After successful model establishment, rats with MIRI were transfected with mimics or inhibitors of miR-1, or siRNA against MAPK3, and then were injected with sevoflurane. A luciferase reporter gene assay was conducted to evaluate the targeting relationship between miR-1 and MAPK3. Reverse transcription quantitative polymerase chain reaction and Western blot analysis were employed to evaluate the expressions of miR-1, MAPK3, PI3K, and Akt. Additionally, the concentration of lactate dehydrogenase (LDH) was determined. Cell apoptosis and viability were assessed using TUNEL and CCK-8 assays, and the ischemic area at risk and infarct size were detected using Evans Blue and TTC staining. MAPK3 was found to be the target gene of miR-1. Mi...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research