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Source: Am J Physiol Cell Ph...

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Total 155 results found since Jan 2013.

Neurogranin is expressed in mammalian skeletal muscle and inhibits calcineurin signaling and myoblast fusion.
Abstract Calcineurin is a Ca2+/calmodulin(CaM)-dependent phosphatase that plays a critical role in promoting the slow fiber phenotype and myoblast fusion in skeletal muscle, thereby making calcineurin an attractive cellular target for enhancing fatigue resistance, muscle metabolism, and muscle repair. Neurogranin (Ng) is a CaM-binding protein thought to be expressed solely in brain and neurons, where it inhibits calcineurin signaling by sequestering CaM, thus lowering its cellular availability. Here, we demonstrate for the first time the expression of Ng protein and mRNA in mammalian skeletal muscle. Both protein ...
Source: Am J Physiol Cell Ph... - August 20, 2019 Category: Cytology Authors: Fajardo VA, Watson CJF, Bott KN, Moradi F, Maddalena LA, Bellissimo CA, Turner KD, Peters SJ, LeBlanc PJ, MacNeil AJ, Stuart JA, Tupling AR Tags: Am J Physiol Cell Physiol Source Type: research

The N6-Methyladenosine (m6A)-Forming Enzyme METTL3 Facilitates M1 Macrophage Polarization through the Methylation of STAT1 mRNA.
In conclusion, METTL3 drives M1 macrophage polarization by directly methylating STAT1 mRNA, potentially serving as an anti-inflammatory target. PMID: 31365297 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - July 30, 2019 Category: Cytology Authors: Liu Y, Liu Z, Tang H, Shen Y, Gong Z, Xie N, Zhang X, Wang W, Kong W, Zhou Y, Fu Y Tags: Am J Physiol Cell Physiol Source Type: research

SMCHD1 terminates the first embryonic genome activation event in mouse two-cell embryos and contributes to a transcriptionally repressive state.
Abstract Embryonic genome activation (EGA) in mammals begins with transient expression of a large group of genes (EGA1). Importantly, entry into and exit from the 2C/EGA state is essential for viability. Duxfamily member genes play an integral role in EGA1 by activating other EGA marker genes such as Zscan4family members. We previously reported that Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (Smchd1) is expressed at the mRNA and protein levels in mouse oocytes and early embryos, and that elimination of Smchd1expression inhibits inner cell mass formation, blastocyst formation a...
Source: Am J Physiol Cell Ph... - July 30, 2019 Category: Cytology Authors: Ruebel ML, Vincent KA, Schall PZ, Wang K, Latham KE Tags: Am J Physiol Cell Physiol Source Type: research

Zinc deficiency up-regulates ET-1 mediated secretion and endothelial cell migration through nuclear HIF-1 translocation.
CONCLUSION: Altogether, the results suggest that zinc deficiency up-regulates ET-1 signaling through HIF-1 activation and stimulates endothelial cell migration suggesting an important role of zinc in the vascular consequences of IH and OSA mediated by the HIF-1-ET- signaling. PMID: 31116583 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - May 21, 2019 Category: Cytology Authors: Morand J, Briancon-Marjollet A, Lemarié E, Gonthier B, Arnaud J, Korichneva I, Godin-Ribuot D Tags: Am J Physiol Cell Physiol Source Type: research

Upregulated MicroRNA-141 Suppresses Epithelial-Mesenchymal Transition and Lymph Node Metastasis via HOXC6-Dependent TGF- β Signaling Pathway in Laryngeal Cancer.
This study investigated the roles of microRNA-141 (miR-141), Homeobox C6 (HOXC6), and the transforming growth factor-β (TGF-β) signaling pathway in epithelial-mesenchymal transition (EMT) and lymph node metastasis in laryngeal cancer. Initially, differentially expressed genes related to laryngeal cancer were retrieved, from which HOXC6 was identified. Next, HOXC6 was determined to be a target gene of miR-141. The regulatory relationship of miR-141, HOXC6 and the TGF-β signaling pathway were then explored accordingly. To further investigate the associated underlying mechanism, Hep-2 cells were transfected with miR-141 mi...
Source: Am J Physiol Cell Ph... - April 23, 2019 Category: Cytology Authors: Sun DZ, Wang XH, Fu YG, Yang PZ, Lv HQ, Yin YW, Zhang XY Tags: Am J Physiol Cell Physiol Source Type: research

Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.
Abstract The apically localized riboflavin (RF) transporter-3 (RFVT-3) is involved in intestinal absorption of vitamin B2. Previous studies have characterized different physiological/biological aspects of the RFVT-3, but there is a lack of knowledge regarding possible existence of interacting partner(s) and consequence of interaction (s) on its function/cell biology. To address the latter, we performed yeast two-hybrid (Y2H) screening of a human colonic cDNA library and have identified transmembrane protein 237 (TMEM237) as a putative interactor with the human (h)RFVT-3; the interaction was further confirmed via "...
Source: Am J Physiol Cell Ph... - March 19, 2019 Category: Cytology Authors: Sabui S, Subramanian VS, Pham Q, Said HM Tags: Am J Physiol Cell Physiol Source Type: research

Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1.
Abstract Defective tight junction (TJ) barrier is a key pathogenic factor for Inflammatory Bowel Disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy related protein ATG6/beclin 1, a key protein in the autophagy pathway, also plays a role in the endocytic pathway. The constitutive role of beclin 1 in intestinal TJ barrier is not known. In Caco-2 cells, beclin 1 was found to be co-immunoprecipitated with the TJ protein occludin and co-localized with occludin on the membrane. Treatment of Caco-2 cells with beclin 1 peptide (Tat-be...
Source: Am J Physiol Cell Ph... - March 19, 2019 Category: Cytology Authors: Wong M, Ganapathy AS, Suchanec E, Laidler L, Ma T, Nighot PK Tags: Am J Physiol Cell Physiol Source Type: research

Mechanisms of Niemann Pick type C1 Like 1 Protein Degradation in Intestinal Epithelial Cells.
In conclusion, our results showed that basal level of intestinal cholesterol transporter NPC1L1 protein is modulated by both ubiquitin-proteasome and lysosome-dependent degradation as well as by ERK1/2-dependent pathway. The modulation of these pathways may provide novel clues for therapeutic intervention to inhibit cholesterol absorption and lower plasma cholesterol. PMID: 30789754 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - February 21, 2019 Category: Cytology Authors: Malhotra P, Soni V, Yamanashi Y, Takada T, Suzuki H, Gill RK, Saksena S, Dudeja PK, Alrefai WA Tags: Am J Physiol Cell Physiol Source Type: research

Polymerase delta interacting protein 2 activates the RhoGEF Epithelial cell transforming sequence 2 in vascular smooth muscle cells.
In this study, we aimed to better understand how Poldip2 activates Rho family GTPases and the functions of the involved proteins in vascular smooth muscle cells (VSMCs). RhoA is activated by guanine nucleotide exchange factors (GEFs). Using nucleotide-free RhoA (isolated from bacteria) to pulldown active RhoGEFs, we found that the RhoGEF Epithelial cell transforming sequence 2 (Ect2) is activated by Poldip2. Ect2 is a critical RhoGEF for Poldip2-mediated RhoA activation, because siRNA against Ect2 prevented Poldip2-mediated RhoA activity (measured by rhotekin pulldowns). Surprisingly, we were unable to detect a direct inte...
Source: Am J Physiol Cell Ph... - February 6, 2019 Category: Cytology Authors: Huff LP, Kikuchi DS, Faidley E, Forrester SJ, Tsai MZ, Lassègue B, Griendling KK Tags: Am J Physiol Cell Physiol Source Type: research

TRPV2 channels mediate insulin secretion induced by cell swelling in mouse pancreatic β-cells.
Abstract β-Cell swelling induces membrane depolarization, which has been suggested to be caused at least partly by the activation of cation channels. Here, we show the identification of the cation channels. In isolated mouse pancreatic β-cells, the exposure to 30% hypotonic solution elicited an increase in cytosolic Ca2+ concentration ([Ca2+]c). The [Ca2+]c elevation was partially inhibited by ruthenium red, a blocker of several Ca2+-permeable channels including transient receptor potential vanilloid receptors (TRPV), and by nicardipine, but not by the depletion of intracellular Ca2+ stores with thapsigargin and...
Source: Am J Physiol Cell Ph... - January 16, 2019 Category: Cytology Authors: Sawatani T, Kaneko YK, Doutsu I, Ogawa A, Ishikawa T Tags: Am J Physiol Cell Physiol Source Type: research

Coagulation factor XI induces Ca2+ response and accelerates cell migration in vascular smooth muscle cells via proteinase-activated receptor 1.
In conclusion, FXIa mainly elicits Ca2+ signal via the PAR1/CaV1.2-mediated Ca2+ influx and accelerates the migration in vascular smooth muscle cells. The present study provides the first evidence that FXIa exerts a direct cellular effect on vascular smooth muscle. PMID: 30566391 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - December 19, 2018 Category: Cytology Authors: Liu W, Hashimoto T, Yamashita T, Hirano K Tags: Am J Physiol Cell Physiol Source Type: research

MicroRNA-143 inhibits proliferation and promotes apoptosis of thymocyte by targeting CXCL13 in myasthenia gravis mice models.
Abstract Myasthenia gravis (MG) is an autoimmune neuromuscular disorder, affecting the quality of life of millions of people worldwide. The current study aims to determine the relationship between microRNA-143 (miR-143) and CXCL13, and whether it influences the pathogenesis of myasthenia gravis (MG). Thymus specimens were resected from patients with thymic hyperplasia combined MG, and then infused into normal mouse cavities to establish MG mice models. Immunohistochemistry, RT-qPCR, in situ hybridization detection, and Western blot analysis were employed to identify the expression of miR-143 and CXCL13 in MG and n...
Source: Am J Physiol Cell Ph... - November 7, 2018 Category: Cytology Authors: Wu DM, Wen X, Han XR, Wang S, Wang YJ, Shen M, Fan SH, Zhuang J, Zhang ZF, Shan Q, Li MQ, Hu B, Sun CH, Lu J, Zheng YL Tags: Am J Physiol Cell Physiol Source Type: research

Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/ β-catenin signaling pathway.
Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway. Am J Physiol Cell Physiol. 2018 Nov 01;315(5):C675-C686 Authors: Gao D, Chen HQ Abstract Metastatic cutaneous squamous cell carcinoma (CSCC) is a major cause of death associated with nonmelanoma skin cancer. The involvement of homeobox B7 ( HOXB7) in cancers has been reported. Thus, the current study intends to explore the effect of HOXB7 on CSCC and its relationship with the Wnt/β-catenin signaling pathway. Initially, microarray-based g...
Source: Am J Physiol Cell Ph... - November 1, 2018 Category: Cytology Authors: Gao D, Chen HQ Tags: Am J Physiol Cell Physiol Source Type: research

USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway.
In conclusion, these results suggest that USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. PMID: 30281322 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - October 3, 2018 Category: Cytology Authors: Chen Q, Hang Y, Zhang T, Tan L, Li S, Jin Y Tags: Am J Physiol Cell Physiol Source Type: research

Copper Transporter ATP7A Interacts with IQGAP1, a Rac1 Binding Scaffold Protein: Role in PDGF-induced VSMC Migration and Vascular Remodeling.
Abstract Vascular smooth muscle cell (VSMC) migration contributes to neointimal formation after vascular injury. We previously demonstrated that copper (Cu) transporter ATP7A is involved in PDGF-induced VSMC migration in Cu- and Rac1-dependent manner. Underlying mechanism is still unknown. Here we show that ATP7A interacts with IQGAP1, a Rac1- and receptor tyrosine kinase-binding scaffold proteins, which mediates PDGF-induced VSMC migration and vascular remodeling. In cultured rat aortic SMCs, PDGF stimulation rapidly promoted ATP7A association with IQGAP1 and Rac1, and their translocation to lipid rafts and leadi...
Source: Am J Physiol Cell Ph... - September 26, 2018 Category: Cytology Authors: Ashino T, Kohno T, Sudhahar V, Ash D, Ushio-Fukai M, Fukai T Tags: Am J Physiol Cell Physiol Source Type: research