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Condition: Back Pain

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Total 191 results found since Jan 2013.

PKM2 is involved in neuropathic pain by regulating ERK and STAT3 activation in rat spinal cord
ConclusionOur findings demonstrate that inhibiting PKM2 expression effectively attenuates CCI-induced neuropathic pain and inflammatory responses in rats, possibly through regulating ERK and STAT3 signaling pathway.
Source: The Journal of Headache and Pain - January 18, 2018 Category: Neurology Source Type: research

Downregulation of spinal astrocytic connexin43 leads to upregulation of interleukin ‐6 and cyclooxygenase‐2 and mechanical hypersensitivity in mice
Abstract Connexin43 (Cx43), involved in intercellular signaling, is expressed in spinal dorsal horn astrocytes and crucial in the maintenance of neuropathic pain. Downregulation of spinal astrocytic Cx43 in mice enhances glutamatergic neurotransmission by decreasing glutamate transporter GLT‐1 expression, resulting in cutaneous hypersensitivity. Decreased expression of astrocytic Cx43 could lead to altered expression of other nociceptive molecules. Transfection of Cx43‐targeting siRNA in cultured spinal astrocytes increased expression of the pronociceptive cytokine interleukin‐6 (IL‐6) and the prostaglandin synthes...
Source: Glia - November 6, 2017 Category: Neurology Authors: Norimitsu Morioka, Shiori Fujii, Syun Kondo, Fang Fang Zhang, Kazuki Miyauchi, Yoki Nakamura, Kazue Hisaoka ‐Nakashima, Yoshihiro Nakata Tags: RESEARCH ARTICLE Source Type: research

Fascin-1 Contributes to Neuropathic Pain by Promoting Inflammation in Rat Spinal Cord.
Abstract Neuropathic pain is a complicated clinical syndrome caused by heterogeneous etiology. Despite the fact that the underlying mechanisms remain elusive, it is well accepted that neuroinflammation plays a critical role in the development of neuropathic pain. Fascin-1, an actin-bundling protein, has been proved to be involved in the processing of diverse biological events including cellular development, immunity, and tumor invasion etc. Recent studies have shown that Fascin-1 participates in antigen presentation and the regulation of pro-inflammatory agents. However, whether Fascin-1 is involved in neuropathic...
Source: Neurochemical Research - October 19, 2017 Category: Neuroscience Authors: Wang B, Fan B, Dai Q, Xu X, Jiang P, Zhu L, Dai H, Yao Z, Xu Z, Liu X Tags: Neurochem Res Source Type: research

i-Fect Deliver Plasmids to the CNS
Important for Gene Expression Studies.I have posted many examples of how our customers usei-FectTM  and otherTransfection Solutions for Gene Manipulation Studies. There are also manypublications.Here we feature how i-Fect was used to delivery plasmids to the CNS: Sara Elramah, Mar ía José López-González, Matthieu Bastide, Florence Dixmérias, Olivier Roca-Lapirot, Anne-Cécile Wielanek-Bachelet, Anne Vital, Thierry Leste-Lasserre, Alexandre Brochard, Marc Landry& Alexandre Favereaux.Spinal miRNA-124 regulates synaptopodin and nociception in an animal model of bone cancer pain. Scientific Reports 7, A...
Source: siRNA and DsiRNA Transfection Efficiency - September 21, 2017 Category: Neuroscience Tags: C iFect Plasmid Transfection Plasmid Vector shRNA plasmids Source Type: news

Cutaneous inflammation regulates THIK1 expression in small C-like nociceptor dorsal root ganglion neurons
In this study we aimed to identify the currently unknown DRG subpopulations expressing THIK1, and to investigate the relationship between the channel and both inflammatory and spontaneous pain in normal rats. Using a combination of immunohistochemistry, western blotting and behavioural tests, we found that all small neurons and large groups of medium and large DRG neurons express THIK1. Myelinated and unmyelinated fibers, nerve endings in the skin and lamina I and II of the spinal cord also express the channel. THIK1 staining co-localizes with IB4-binding and trkA suggesting that the channel is expressed by nociceptors. At...
Source: Molecular and Cellular Neuroscience - July 2, 2017 Category: Neuroscience Source Type: research

MiR-210 facilitates ECM degradation by suppressing autophagy via silencing of ATG7 in human degenerated NP cells
Publication date: September 2017 Source:Biomedicine & Pharmacotherapy, Volume 93 Author(s): Cheng Wang, Zi-Zhen Zhang, Wei Yang, Zhi-Hua Ouyang, Jing-Bo Xue, Xue-Lin Li, Jian Zhang, Wen-Kang Chen, Yi-Guo Yan, Wen-Jun Wang Intervertebral disc degeneration (IDD) is thought to be the most common cause of low back pain. Dysregulation of microRNAs (miRNAs) is involved in the development of IDD. The aim of this study was to explore the influence of miR-210 on type II collagen (Col II) and aggrecan expression and possible mechanisms in human degenerated nucleus pulposus (NP) cells. Our results showed that miR-210 levels ...
Source: Biomedicine and Pharmacotherapy - June 29, 2017 Category: Drugs & Pharmacology Source Type: research

MiR-210 facilitates ECM degradation by suppressing autophagy via silencing of ATG7 in human degenerated NP cells.
Abstract Intervertebral disc degeneration (IDD) is thought to be the most common cause of low back pain. Dysregulation of microRNAs (miRNAs) is involved in the development of IDD. The aim of this study was to explore the influence of miR-210 on type II collagen (Col II) and aggrecan expression and possible mechanisms in human degenerated nucleus pulposus (NP) cells. Our results showed that miR-210 levels were significantly increased in degenerated NP tissues compared with healthy controls, and positively correlated with disc degeneration grade. By gain-of-function and loss-of-function studies in human degenerated ...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - June 28, 2017 Category: Drugs & Pharmacology Authors: Wang C, Zhang ZZ, Yang W, Ouyang ZH, Xue JB, Li XL, Zhang J, Chen WK, Yan YG, Wang WJ Tags: Biomed Pharmacother Source Type: research

Recent Advances in the Development of T ‐type Calcium Channel Blockers for Pain Intervention
Abstract Cav3.2 T‐type calcium channels are important regulators of pain signals in afferent pain pathway, and their activities are dysregulated during various chronic pain states. Therefore it stands to reason that inhibiting T‐type calcium channels in dorsal root ganglion neurons and in the spinal dorsal horn can be targeted for pain relief. This is supported by early pharmacological studies with T‐type channel blockers such as ethosuximide, and by analgesic effects of siRNA depletion of Cav3.2 channels. In the past five years, considerable effort has been applied towards identifying novel classes of T‐type calci...
Source: British Journal of Pharmacology - June 13, 2017 Category: Drugs & Pharmacology Authors: Terrance P Snutch, Gerald W Zamponi Tags: REVIEW ARTICLE THEMED ISSUE Source Type: research

Epigenetic upregulation of CXCL12 expression mediates antitubulin chemotherapeutics–induced neuropathic pain
Abstract: Clinically, Microtubule-targeted agents–induced neuropathic pain hampers chemotherapeutics for patients with cancer. Here, we found that application of paclitaxel or vincristine increased the protein and mRNA expression of CXCL12 and frequency and amplitude of miniature excitatory post synaptic currents (mEPSCs) in spinal dorsal horn neurons. Spinal local application of CXCL12 induced the long-term potentiation of nociceptive synaptic transmission and increased the amplitude of mEPSCs. Inhibition of CXCL12 using the transgenic mice (CXCL12−/+) or neutralizing antibody or siRNA ameliorated the mEPSC's enhancem...
Source: Pain - March 25, 2017 Category: Anesthesiology Tags: Research Paper Source Type: research

Cynandione A attenuates neuropathic pain through p38 β MAPK-mediated spinal microglial expression of β-endorphin
This study aims to evaluate the antihypersensitivity activities of cynandione A in neuropathy and explored its mechanisms of action. Intrathecal injection of cynandione A dose-dependently attenuated spinal nerve ligation-induced mechanical allodynia and thermal hyperalgesia, with maximal possible effects of 57% and 59%, ED50s of 14.9 μg and 6.5 μg, respectively. Intrathecal injection of cynandione A significantly increased β-endorphin levels in spinal cords of neuropathic rats and its treatment concentration-dependently induced β-endorphin expression in cultured primary microglia (but not in neurons or astrocytes), wit...
Source: Brain, Behavior, and Immunity - February 8, 2017 Category: Neurology Source Type: research

Cynandione A attenuates neuropathic pain through p38 β MAPK-mediated spinal microglial expression of β-endorphin.
This study aims to evaluate the antihypersensitivity activities of cynandione A in neuropathy and explored its mechanisms of action. Intrathecal injection of cynandione A dose-dependently attenuated spinal nerve ligation-induced mechanical allodynia and thermal hyperalgesia, with maximal possible effects of 57% and 59%, ED50s of 14.9 μg and 6.5 μg, respectively. Intrathecal injection of cynandione A significantly increased β-endorphin levels in spinal cords of neuropathic rats and its treatment concentration-dependently induced β-endorphin expression in cultured primary microglia (but not in neurons or astrocytes), wit...
Source: Brain, Behavior, and Immunity - February 7, 2017 Category: Neurology Authors: Huang Q, Mao XF, Wu HY, Liu H, Sun ML, Wang X, Wang YX Tags: Brain Behav Immun Source Type: research

Activation of NPFFR2 leads to hyperalgesia through the spinal inflammatory mediator CGRP in mice.
Abstract Neuropeptide FF (NPFF) is recognized as an opioid modulating peptide that regulates morphine-induced analgesia. The aim of this study was to delineate the role of NPFFR2 in pain transmission. We found the expression levels of NPFF and NPFFR2 were increased in the lumbar dorsal horn of animals with CFA- and carrageenan-induced inflammation and both NPFFR2 over-expressing transgenic (NPFFR2-Tg) and NPFFR2 agonist-treated mice displayed hyperalgesia. BOLD signals from functional MRI showed that NPFFR2-Tg mice exhibited increased activation of pain-related brain regions after painful stimulation when compared...
Source: Experimental Neurology - February 4, 2017 Category: Neurology Authors: Lin YT, Liu HL, Day YJ, Chang CC, Hsu PH, Chen JC Tags: Exp Neurol Source Type: research

CaMKIIα underlies spontaneous and evoked pain behaviors in Berkeley sickle cell transgenic mice
This study aimed to investigate the functional involvement of Ca2+/calmodulin-dependent protein kinase II (CaMKIIα) in the persistent and refractory pain associated with SCD. We found that nonevoked ongoing pain as well as evoked hypersensitivity to mechanical and thermal stimuli were present in Berkeley sickle cell transgenic mice (BERK mice), but not nonsickle control littermates. Prominent activation of CaMKIIα was observed in the dorsal root ganglia and spinal cord dorsal horn region of BERK mice. Intrathecal administration of KN93, a selective inhibitor of CaMKII, significantly attenuated mechanical allodynia and he...
Source: Pain - November 22, 2016 Category: Anesthesiology Tags: Research Paper Source Type: research

Autophagy activated by tuberin/mTOR/p70S6K suppression is a protective mechanism against local anaesthetics neurotoxicity.
Abstract The local anaesthetics (LAs) are widely used for peripheral nerve blocks, epidural anaesthesia, spinal anaesthesia and pain management. However, exposure to LAs for long duration or at high dosage can provoke potential neuronal damages. Autophagy is an intracellular bulk degradation process for proteins and organelles. However, both the effects of LAs on autophagy in neuronal cells and the effects of autophagy on LAs neurotoxicity are not clear. To answer these questions, both lipid LAs (procaine and tetracaine) and amide LAs (bupivacaine, lidocaine and ropivacaine) were administrated to human neuroblasto...
Source: J Cell Mol Med - November 14, 2016 Category: Molecular Biology Authors: Xiong J, Kong Q, Dai L, Ma H, Cao X, Liu L, Ding Z Tags: J Cell Mol Med Source Type: research

Down-regulation of zinc transporter-1 in astrocytes induces neuropathic pain via the brain-derived neurotrophic factor - K+-Cl? co-transporter-2 signaling pathway in the mouse spinal cord.
Publication date: Available online 3 November 2016 Source:Neurochemistry International Author(s): Tomoya Kitayama, Katsuya Morita, Naoyo Motoyama, Toshihiro Dohi We previously demonstrated, using a DNA microarray analysis, the down-regulated expression of the slc30a1 gene (zinc transporter 1, ZnT1) in a neuropathic pain model induced by partial sciatic nerve ligation (PSNL). Zinc is an essential trace mineral that plays important roles in physiological functions, and ZnT1 modulates intracellular zinc levels. In the present study, we examined the effects of the down-regulation of the ZnT1 gene in the spinal cord on tactile...
Source: Neurochemistry International - November 2, 2016 Category: Neuroscience Source Type: research