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Condition: Back Pain
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Total 191 results found since Jan 2013.
Downregulation of connexin36 in mouse spinal dorsal horn neurons leads to mechanical allodynia
Connexin36 (Cx36), a component of neuronal gap junctions, is crucial for interneuronal communication and regulation. Gap junction dysfunction underlies neurological disorders, including chronic pain. Following a peripheral nerve injury, Cx36 expression in the ipsilateral spinal dorsal horn was markedly decreased over time, which paralleled the time course of hind paw tactile allodynia. Intrathecal (i.t.) injection of Cx36 siRNA (1 and 5 pg) significantly reduced the expression of Cx36 protein in the lumbar spinal cord, peaking 3 days after the injection, which corresponded with the onset of hind paw tactile allodynia. It i...
Source: Journal of Neuroscience Research - November 14, 2014 Category: Neuroscience Authors: Yoki Nakamura, Norimitsu Morioka, Fang Fang Zhang, Kazue Hisaoka‐Nakashima, Yoshihiro Nakata Tags: Research Article Source Type: research
BDNF contributes to the development of neuropathic pain by induction of spinal long-term potentiation via SHP2 associated GluN2B-containing NMDA receptors activation in rats with spinal nerve ligation.
In this study, we investigated whether spinal BDNF contributes to dorsal horn LTP induction and neuropathic pain development by activation of GluN2B-NMDA receptors via Src homology-2 domain-containing protein tyrosine phosphatase-2 (SHP2) phosphorylation in rats following spinal nerve ligation (SNL). We first demonstrated that spinal BDNF participates in the development of long-lasting hyperexcitability of dorsal horn WDR neurons (i.e. central sensitization) as well as pain allodynia in both intact and SNL rats. Second, we revealed that BDNF induces spinal LTP at C-fiber synapses via functional up-regulation of GluN2B-NMDA...
Source: Neurobiology of Disease - November 8, 2014 Category: Neurology Authors: Ding X, Cai J, Li S, Liu XD, Wan Y, Xing GG Tags: Neurobiol Dis Source Type: research
Effect of siRNA interference on nerve growth factor in intervertebral disc inflammation rats
Conclusions These molecular biological results suggest that inflammatory factor IL-6 and IL-1β could stimulate NGF on intervertebral disc cells in vitro culture model and its efficiency is concentration dependent, while siRNA interference can inhibit the stimulation effect of IL-6 and IL-1β on intervertebral disc cell, which provides a new targets for the clinical treatment of discogenic low back pain.
Source: Asian Pacific Journal of Tropical Medicine - October 12, 2014 Category: Tropical Medicine Source Type: research
TRAF6 upregulation in spinal astrocytes maintains neuropathic pain by integrating TNF-α and IL-1β signaling
In this study, we investigated the role of TRAF6 in neuropathic pain in mice after spinal nerve ligation (SNL). SNL induced persistent TRAF6 upregulation in the spinal cord. Interestingly, TRAF6 was mainly colocalized with the astrocytic marker glial fibrillary acidic protein on SNL day 10 and partially expressed in microglia on SNL day 3. In cultured astrocytes, TRAF6 was upregulated after exposure to TNF-α or IL-1β. TNF-α or IL-1β also increased CCL2 expression, which was suppressed by both siRNA and shRNA targeting TRAF6. TRAF6 siRNA treatment also inhibited the phosphorylation of c-Jun N-terminal kinase (JNK) in as...
Source: Pain - September 29, 2014 Category: Anesthesiology Authors: Ying Lu, Bao-Chun Jiang, De-Li Cao, Zhi-Jun Zhang, Xin Zhang, Ru-Rong Ji, Yong-Jing Gao Tags: Research papers Source Type: research
NRG1‐ErbB signalling promotes microglia activation contributing to incision‐induced mechanical allodynia
ConclusionIncision‐induced NRG1 expression mediated activation of dorsal horn microglia and contributed to the development of mechanical allodynia. Specifically targeting NRG1‐ErbB signalling may therefore provide a new therapeutic intervention for relieving incision‐induced mechanical allodynia.
Source: European Journal of Pain - August 27, 2014 Category: Anesthesiology Authors: Y. Xiang, T. Liu, H. Yang, F. Gao, H. Xiang, A. Manyande, Y. Tian, X. Tian Tags: Original Article Source Type: research
Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news
Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news
Pain Research Gene Expression Analysis
Potent and Proven Transfection KitsPain Researchers have successfully modulated 25+ genes involved in pain pathways using our Transfection Kits. Highlights include: DOR,The β3 subunit of Na+,K+-ATPase, NTS1, NAV1.8, Kv 1.1, Kv 9.1, TROY, NOV, β-arrestin, TRPV1, CAV1.2, TLR4 and ASIC and more! To learn more, check out our Transfection Kit Publications and Blog.Figures: Intrathecal Kv9.1 siRNA treatment induces pain behaviors in naive rats. A, qRT-PCR quantification of Kv9.1 mRNA in rat PASMC cultures transfected with one of three Kv9.1 siRNA sequences or control siRNA. B, qRT-PCR showing Kv9.1 in vivo knock-do...
Source: Neuromics - July 23, 2014 Category: Neuroscience Tags: chronic pain gene expression analysis Gene Silencing i-Fect inflammatory nociception Nociceptin Pain Research siRNA delivery in-vivo Source Type: news
P2X4 receptor in the dorsal horn partially contributes to brain‐derived neurotrophic factor oversecretion and toll‐like receptor‐4 receptor activation associated with bone cancer pain
Previous studies have suggested that the microglial P2X7 purinoceptor is involved in the release of tumor necrosis factor‐α (TNFα) following activation of toll‐like receptor‐4 (TLR4), which is associated with nociceptive behavior. In addition, this progress is evoked by the activation of the P2X4 purinoceptor (P2X4R). Although P2X4R is also localized within spinal microglia in the dorsal horn, little is known about its role in cancer‐induced bone pain (CIBP), which is in some ways unique. With the present rat model of CIBP, we demonstrate a critical role of the microglial P2X4R in the enhanced nociceptive transmi...
Source: Journal of Neuroscience Research - July 1, 2014 Category: Neuroscience Authors: Xiao‐hong Jin, Li‐Na Wang, Jian‐Ling Zuo, Jian‐Ping Yang, Si‐lan Liu Tags: Research Article Source Type: research
Native store-operated calcium channels are functionally expressed in mouse spinal cord dorsal horn neurons and regulate resting calcium homeostasis.
This article is protected by copyright. All rights reserved.
PMID: 24860175 [PubMed - as supplied by publisher]
Source: The Journal of Physiology - May 23, 2014 Category: Physiology Authors: Xia J, Pan R, Gao X, Meucci O, Hu H Tags: J Physiol Source Type: research
Geniposide and its iridoid analogs exhibit antinociception by acting at the spinal GLP-1 receptors.
This study evaluated the antinociceptive activities of geniposide, a presumed small molecule GLP-1R agonist. Geniposide produced concentration-dependent, complete protection against hydrogen peroxide-induced oxidative damage in PC12 and HEK293 cells expressing rat and human GLP-1Rs, but not in HEK293T cells that do not express GLP-1Rs. The orthosteric GLP-1R antagonist exendin(9-39) right-shifted the concentration-response curve of geniposide without changing the maximal protection, with identical pA2 values in both cell lines. Subcutaneous and oral geniposide dose-dependently blocked the formalin-induced tonic response bu...
Source: Neuropharmacology - April 17, 2014 Category: Drugs & Pharmacology Authors: Gong N, Fan H, Ma AN, Xiao Q, Wang YX Tags: Neuropharmacology Source Type: research
Irritable Bowel Syndrome (IBS) and BDNF
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: Neuromics - March 20, 2014 Category: Neuroscience Tags: BDNF i-Fect Source Type: news
i-Fect, BDNF and Irritable Bowel Syndrome (IBS)
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: siRNA and DsiRNA Transfection Efficiency - March 11, 2014 Category: Neuroscience Tags: BDNF;chronic stress;functional bowel disorders;iritable bowel syndrome;visceral hypersnsitivity i-Fect intrathecal delivery of siRNA Source Type: news
Extracellular caspase-6 drives murine inflammatory pain via microglial TNF-α secretion
Increasing evidence indicates that the pathogenesis of neuropathic pain is mediated through spinal cord microglia activation. The intracellular protease caspase-6 (CASP6) is known to regulate neuronal apoptosis and axonal degeneration; however, the contribution of microglia and CASP6 in modulating synaptic transmission and pain is unclear. Here, we found that CASP6 is expressed specifically in C-fiber axonal terminals in the superficial spinal cord dorsal horn. Animals exposed to intraplantar formalin or bradykinin injection exhibited CASP6 activation in the dorsal horn. Casp6-null mice had normal baseline pain, but impair...
Source: Journal of Clinical Investigation - February 17, 2014 Category: Biomedical Science Authors: Temugin Berta, Chul-Kyu Park, Zhen-Zhong Xu, Ruo-Gang Xie, Tong Liu, Ning Lü, Yen-Chin Liu, Ru-Rong Ji Source Type: research
Menin regulates spinal glutamate-GABA balance through GAD65 contributing to neuropathic pain.
CONCLUSION: Our findings provide mechanistic insight into the contribution of the upregulated spinal menin to peripheral nerve injury induced neuropathic hypersensitivity by regulating glutamate-GABA balance through deactivating GAD65.
PMID: 24905306 [PubMed - as supplied by publisher]
Source: Pharmacological Reports - February 1, 2014 Category: Drugs & Pharmacology Authors: Shen X, Liu Y, Xu S, Zhao Q, Wu H, Guo X, Shen R, Wang F Tags: Pharmacol Rep Source Type: research
