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Condition: Obesity

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Total 449 results found since Jan 2013.

High Fat and Diet Induced Obesity
i-FectTM Delivers Again!Research shows that rats and humans on a high-fat diet (HFD) are less sensitive to satiety signals known to act via vagal afferent pathways. Impaired vagal afferent responsiveness to both gastric satiety hormones (CCK and leptin) and mechanical stimulation raises the possibility that changes in electrophysiological properties may be the underlying mechanism responsible for impaired vagal responsiveness to a wide variety of satiety signals.Potassium channels play a central role. To demonstrate this researchers used ouri-Fect siRNA Transfection Kit to silence TRESK and TASK1 to understand their impact...
Source: siRNA and DsiRNA Transfection Efficiency - September 16, 2019 Category: Neuroscience Tags: iFect in vivo siRNA intrathecal delivery of siRNA RNAi TRESK Source Type: news

Prolonged efficiency of siRNA-mediated gene silencing in primary cultures of human preadipocytes and adipocytes.
CONCLUSIONS: Our study describes the methods of gene silencing in primary cultures of human preadipocytes and adipocytes and their prolonged effectiveness. PMID: 24307633 [PubMed - as supplied by publisher]
Source: Obesity - December 5, 2013 Category: Eating Disorders and Weight Management Authors: Lee MJ, Pickering RT, Puri V Tags: Obesity (Silver Spring) Source Type: research

A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction
Biomaterials. 2022 Apr 28;285:121541. doi: 10.1016/j.biomaterials.2022.121541. Online ahead of print.ABSTRACTObesity is the major risk factor for metabolic diseases such as fatty liver, hyperlipidemia and insulin resistance. Beige fat has been recognized as a therapeutic target considering its great potential to burn energy. Since the evolutionary discovery of RNA interference and its utilization for gene knockdown in mammalian cells, a remarkable progress has been achieved in siRNA-based therapeutics. However, efficient delivery of siRNA into adipose tissues or differentiated adipocytes is challenging due to high lipid co...
Source: Biomaterials - May 9, 2022 Category: Materials Science Authors: Jin Qiu Qianqian Fan Sainan Xu Dongmei Wang Juntong Chen Sainan Wang Tianhui Hu Xinran Ma Yiyun Cheng Lingyan Xu Source Type: research

Therapeutic silencing of HPV 16 E7 by systemic administration of siRNA-neutral DOPC nanoliposome in a murine cervical cancer model with obesity.
CONCLUSION: There was no negative impact of obesity on the antitumor activity of siRNA-DOPC-NP in obese mice. PMID: 26854443 [PubMed - as supplied by publisher]
Source: Journal of B.U.ON. - February 9, 2016 Category: Cancer & Oncology Tags: J BUON Source Type: research

Treatment of the metabolic syndrome by siRNA targeting apolipoprotein CIII
AbstractApolipoprotein CIII (apoCIII) is increased in obesity-induced insulin resistance and type-2 diabetes. Emerging evidences support the advantages of small interfering RNAs (siRNAs) to target disease-causing genes. The aim of this study was to develop siRNAs for in vivo silencing of apoCIII and investigate if this results in metabolic improvements comparable to what we have seen using antisense oligonucelotides against apoCIII. Twenty-four siRNAs were synthesized and tested in a dual luciferase reporter assay. The eight best were selected, based on knockdown at 20  nM, and of these, two were selected based on IC50 v...
Source: BioFactors - August 31, 2022 Category: Biochemistry Authors: Patricia Recio ‐López, Ismael Valladolid‐Acebes, Philipp Hadwiger, Markus Hossbach, Monika Krampert, Carla Prata, Per‐Olof Berggren, Lisa Juntti‐Berggren Tags: RESEARCH COMMUNICATION Source Type: research

Leptin siRNA promotes ovarian granulosa cell apoptosis and affects steroidogenesis by increasing NPY2 receptor expression.
In conclusion, knockdown of leptin significantly affected ovarian steroidogenesis and ovarian function through NPY. siLeptin transfection impaired the activation of JAK2/STAT3 and contributed to ovarian granulosa cell apoptosis partially through up-regulating NPY2R expression. PMID: 28864114 [PubMed - as supplied by publisher]
Source: Gene - August 29, 2017 Category: Genetics & Stem Cells Authors: Ding X, Kou X, Zhang Y, Zhang X, Cheng G, Jia T Tags: Gene Source Type: research

Genetic Regulation of Liver Metabolites and Transcripts Linking to Biochemical-Clinical Parameters
Conclusion In summary, this study is the first to combine metabolomics, transcriptomics, and genome-wide association studies in a porcine model. Our results improve understanding of the genetic regulation of metabolites which link to transcripts and finally biochemical-clinical parameters. Further, high-performance profiling of metabolites as intermediate phenotypes is a potentially powerful approach to uncover how genetic variation affects metabolic and health status. Our results advance knowledge in areas of biomedical and agricultural interest and identify potential correlates of biomarkers, SNPs-metabolites, SNPs-tran...
Source: Frontiers in Genetics - April 16, 2019 Category: Genetics & Stem Cells Source Type: research

Tangshen Formula Alleviates Hepatic Steatosis by Inducing Autophagy Through the AMPK/SIRT1 Pathway
Conclusion In conclusion, the present study demonstrated that autophagy was involved in relieving the effects of TSF against NAFLD, which were mediated by the AMPK/SIRT1 pathway (Figure 7D). These findings may improve our current understanding of the role of TSF in treating hepatic steatosis and provide an experimental basis for the clinical application of TSF in NAFLD and its related metabolic syndrome. Ethics Statement This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Ethics Co...
Source: Frontiers in Physiology - April 25, 2019 Category: Physiology Source Type: research

Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression.
Authors: Chang CC, Sia KC, Chang JF, Lin CM, Yang CM, Huang KY, Lin WN Abstract The proliferation and adipogenesis of preadipocytes played important roles in the development of adipose tissue and contributed much to the processes of obesity. On the other hand, lipopolysaccharide (LPS), also known as endotoxin, is a key outer membrane component of gram-negative bacteria in the gut microbiota, and has a dominant role in linking inflammation to high-fat diet-induced metabolic syndrome. Studies suggested the potential roles of LPS in hepatic steatosis and in obese mice models. However, the molecular mechanisms underlyi...
Source: International Journal of Medical Sciences - January 22, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

LncRNA-GAS5 Inhibits Expression of miR 103 and Ameliorates the Articular Cartilage in Adjuvant-Induced Arthritis in Obese Mice.
Abstract We explored whether long noncoding RNA growth arrest-specific transcript 5 (LncRNA-GAS5) small interfering RNA (siRNA) reduced cartilage destruction in obese mice with adjuvant-induced arthritis. We studied the effects of LncRNA-GAS5 siRNA on the polyarthritis index; hind paw swelling; and the serum levels of certain biochemicals, cytokines, and oxidative stress parameters. We measured the expression levels of matrix metalloproteinases (MMP)-13, NF-κB, fibroblast growth factor (FGF) 21, p38, Akt, and PI3K in cartilage via Western blotting and quantitative reverse transcription PCR. Long noncoding RNA-GAS...
Source: Dose Response - October 1, 2020 Category: Drugs & Pharmacology Authors: Chen H, He C, Liu Y, Li X, Zhang C, Qin Q, Pang Q Tags: Dose Response Source Type: research

LncRNA-GAS5 Inhibits Expression of miR 103 and Ameliorates the Articular Cartilage in Adjuvant-Induced Arthritis in Obese Mice
Dose Response. 2020 Dec 9;18(4):1559325820942718. doi: 10.1177/1559325820942718. eCollection 2020 Oct-Dec.ABSTRACTWe explored whether long noncoding RNA growth arrest-specific transcript 5 (LncRNA-GAS5) small interfering RNA (siRNA) reduced cartilage destruction in obese mice with adjuvant-induced arthritis. We studied the effects of LncRNA-GAS5 siRNA on the polyarthritis index; hind paw swelling; and the serum levels of certain biochemicals, cytokines, and oxidative stress parameters. We measured the expression levels of matrix metalloproteinases (MMP)-13, NF-κB, fibroblast growth factor (FGF) 21, p38, Akt, and PI3K in c...
Source: Dose Response - December 21, 2020 Category: Drugs & Pharmacology Authors: Hongwei Chen Chuan He Yan Liu Xiaolin Li Chaoju Zhang Qunyan Qin Qixiong Pang Source Type: research

Lingual CD36 and nutritional status differentially regulate fat preference in obesity-prone and obesity-resistant rats
Publication date: 15 May 2017 Source:Physiology & Behavior, Volume 174 Author(s): H. Douglas Braymer, Hannah Zachary, Allyson L. Schreiber, Stefany D. Primeaux Lingual fatty acid receptors (i.e. CD36) mediate the orosensory perception of fat/fatty acids and may contribute to the susceptibility to develop obesity. The current study tested the hypothesis that fat/fatty acid preference in obesity-prone (OP, Osborne-Mendel) and obesity-resistant (OR, S5B/Pl) rats is mediated by nutritional status and lingual CD36. To determine if nutritional status affected linoleic acid (LA) preference in OP and OR rats, rats were ei...
Source: Physiology and Behavior - March 26, 2017 Category: Physiology Source Type: research

Berberine activates AMPK to suppress proteolytic processing, nuclear translocation and target DNA binding of SREBP-1c in 3T3-L1 adipocytes.
In conclusion, the results of the present study demonstrate that BBR activates AMPK to induce phosphorylation of SREBP‑1c, thereby suppressing proteolytic processing, nuclear translocation and target DNA binding of SREBP‑1c, which leads to a reduction in lipogenic gene expression and intracellular fat accumulation. The results of the present study indicate that BBR may be a potential candidate for the development of drugs to treat obesity. PMID: 28487951 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - May 12, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research