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Condition: Chronic Obstructive Pulmonary

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Total 173 results found since Jan 2013.

siRNA delivery to macrophages using aspherical, nanostructured microparticles as delivery system for pulmonary administration.
Abstract The delivery of oligonucleotides such as siRNA to the lung is a major challenge, as this group of drugs has difficulties to overcome biological barriers due to its polyanionic character and the associated hydrophilic properties, resulting in inefficient delivery. Especially in diseases such as asthma, chronic obstructive pulmonary disease and cystic fibrosis, where increased proinflammation is present, a targeted RNA therapy is desirable due to the high potency of these oligonucleotides. To address these problems and to ensure efficient uptake of siRNA in macrophages, a microparticulate, cylindrical deliv...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 4, 2020 Category: Drugs & Pharmacology Authors: Fischer T, Tschernig T, Drews F, Brix K, Meier C, Simon M, Kautenburger R, Schneider M Tags: Eur J Pharm Biopharm Source Type: research

Spray drying siRNA-lipid nanoparticles for dry powder pulmonary delivery
This study verifies the successful spray drying procedure of LNP-siRNA systems maintaining their integrity and mediating strong gene silencing efficiency on mRNA and protein levels both in vitro and ex vivo. The successful spray drying procedure of LNP-siRNA formulations in 5% lactose solution creates a novel siRNA-based therapy option to target respiratory diseases such as lung cancer, asthma, COPD, cystic fibrosis and viral infections.PMID:36126785 | DOI:10.1016/j.jconrel.2022.09.021
Source: Cancer Control - September 20, 2022 Category: Cancer & Oncology Authors: Christoph M Zimmermann Domizia Baldassi Karen Chan Nathan B P Adams Alina Neumann Diana Leidy Porras-Gonzalez Xin Wei Nikolaus Kneidinger Mircea Gabriel Stoleriu Gerald Burgstaller Dominik Witzigmann Paola Luciani Olivia M Merkel Source Type: research

The effects of INTS12 knockdown on processing of snRNAs
Conclusions: INTS12 is likely to play an important role in HBEC function via snRNA processing: this may affect transcription globally.Funding: Medical Research Council1. Baillat et al. (2005) Cell 123 (2): 265–76.2. Obeidat et al. (2013) PLoSOne 18;8(9): e74630.
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Kheirallah, A. K., Hodge, E., Nelson, C., Sayers, I., Hall, I. Tags: 3.2 Airway Cell Biology and Immunopathology Source Type: research

Small interfering RNA against ERK1/2 attenuates cigarette smoke-induced pulmonary vascular remodeling.
This study investigated the potential role of ERK1/2 in the proliferation of rat PASMCs (rPASMCs) and cigarette smoke-induced PVR in rats. A small interfering RNA (siRNA) against ERK1/2 (ERK1/2-siRNA) was synthesized, and it significantly reduced the expression of ERK1/2 and cyclin E1, significantly increased the proportion of cells arrested at G0/G1 phase and significantly suppressed the proliferation of rPASMCs treated with cigarette smoke extract compared with controls (all P<0.05). In rats, ERK1/2-siRNA, which was administered intranasally, also inhibited the activation of ERK1/2 and the upregulation of cyclin E1, b...
Source: Experimental and Therapeutic Medicine - December 6, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research

Current Drug Targeting using siRNAbased Nano Therapeutics for Pulmonary Diseases
Curr Pharm Des. 2022 Jul 27. doi: 10.2174/1381612828666220727144223. Online ahead of print.ABSTRACTThe importance of siRNA in nano drug delivery systems to target important pulmonary disorders such as chronic obstructive pulmonary disease (COPD), asthma, lung cancer, and others is reviewed in this perspective. The great majority of lung illnesses are caused by protein misfolding. As a result, siRNA-based therapies are increasingly being used to target the gene. Given the difficulties of delivering bare siRNA, siRNA protection may ensure its efficacy in gene therapy. These issues could be solved with a nano-based siRNA deli...
Source: Current Pharmaceutical Design - July 27, 2022 Category: Drugs & Pharmacology Authors: Venkateshwaran Krishnaswami Ruckmani Kandasamy Source Type: research

Cadmium induces mucin 8 expression via Toll‐like receptor 4–mediated extracellular signal related kinase 1/2 and p38 mitogen‐activated protein kinase in human airway epithelial cells
ConclusionThe results of this study suggest for the first time that cadmium induces MUC8 expression via TLR4‐mediated ERK1/2 and p38 MAPK signaling pathway in human airway epithelial cells.
Source: International Forum of Allergy and Rhinology - January 1, 2016 Category: Allergy & Immunology Authors: Si‐Youn Song, Chang Hoon Bae, Yoon Seok Choi, Yong‐Dae Kim Tags: ORIGINAL ARTICLE Source Type: research

Anti-inflammatory effect of anti-tnf- α cationic phosphorous dendrimers nanocomplexes administered intranasally in a murine acute lung injury model.
ANTI-INFLAMMATORY EFFECT OF ANTI-TNF-α CATIONIC PHOSPHOROUS DENDRIMERS NANOCOMPLEXES ADMINISTERED INTRANASALLY IN A MURINE ACUTE LUNG INJURY MODEL. Biomacromolecules. 2017 Jun 22;: Authors: Bohr A, Tsapis N, Andreana I, Chamarat A, Foged C, Delomenie C, Noiray M, El Brahmi N, Majoral JP, Mignani S, Fattal E Abstract Inflammation is an essential component of many lung diseases, including asthma, chronic obstructive pulmonary disease (COPD) or acute lung injury. Our purpose was to design efficient carriers for lung delivery of small interfering RNA (siRNA) targeting tumor necrosis factor (TNF-α) in an...
Source: Biomacromolecules - June 22, 2017 Category: Biochemistry Authors: Bohr A, Tsapis N, Andreana I, Chamarat A, Foged C, Delomenie C, Noiray M, El Brahmi N, Majoral JP, Mignani S, Fattal E Tags: Biomacromolecules Source Type: research

Smad3 mediates cigarette smoke extract (CSE) induction of VEGF release by human fetal lung fibroblasts.
Abstract Cigarette smoke is the major cause of chronic obstructive pulmonary disease (COPD), yet pathogenic mechanisms are not fully understood. Vascular endothelial growth factor (VEGF) is one of the major regulators of endothelial cell survival and is believed to play a role in the pathogenesis of COPD. Fibroblasts are a significant source of VEGF in the lungs; however the effect of cigarette smoke exposure on VEGF release by fibroblasts is not fully understood. We hypothesized that cigarette smoke-induced disturbed VEGF release by human lung fibroblasts is a potential pathogenic mechanism that could contribute ...
Source: Toxicology Letters - April 22, 2013 Category: Toxicology Authors: Farid M, Kanaji N, Nakanishi M, Gunji Y, Michalski J, Iwasawa S, Ikari J, Wang X, Basma H, Nelson AJ, Liu X, Rennard SI Tags: Toxicol Lett Source Type: research

INTS12 knockdown leads to perturbation of genes and pathways of relevance to airway biology
Conclusions: INTS12 regulates pathways important in lung function through perturbation of genes belonging to e.g. collagen formation pathway. INTS12 is likely to control protein synthesis through effects on tRNA aminoacylation pathway.Funding: Medical Research Council.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Kheirallah, A. K., Sayers, I., Hall, I. Tags: 3.1 Molecular Pathology and Functional Genomics Source Type: research

HMGB1 mediates Aspergillus fumigatus-induced inflammatory response in alveolar macrophages of COPD mice via activating MyD88/NF- κB and syk/PI3K signalings.
In conclusion, HMGB1 is responsible for A. fumigatus-induced inflammatory response in COPD alveolar macrophage via Dectin-1 and TLR2/4 receptor through activating MyD88/NF-κB and syk/PI3K signalings. PMID: 29078091 [PubMed - as supplied by publisher]
Source: International Immunopharmacology - October 24, 2017 Category: Allergy & Immunology Authors: Zhang P, Xin X, Fang L, Jiang H, Xu X, Su X, Shi Y Tags: Int Immunopharmacol Source Type: research

Gene Silencing of Receptor-Interacting Protein 2 Protects Against Cigarette Smoke-Induced Acute Lung Injury
CONCLUSIONS AND IMPLICATIONSTaken together, we report for the first time that Rip2 gene silencing ameliorated CS-induced acute lung injury probably via disruption of the NF-κB activity, postulating that Rip2 may be a novel therapeutic target for COPD.Graphical abstract
Source: Pharmacological Research - October 29, 2018 Category: Drugs & Pharmacology Source Type: research

Transcriptional and Epigenetic Modulation of Human Rhinovirus-Induced CXCL10 Production by Cigarette Smoke.
Abstract Human rhinovirus (HRV) triggers exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Cigarette smoking is the primary risk factor for the development of COPD and 25% of asthmatics smoke. Smokers experience both longer and more severe colds. We previously showed that cigarette smoke extract (CSE) inhibited HRV-induced expression of a range of epithelial antiviral molecules. Here, we use CXCL10 as a model antiviral gene to examine the mechanisms by which CSE inhibits epithelial antiviral immunity. HRV-induced CXCL10 transcription depends upon activation of nuclear factor-ĸB (NF-ĸB) an...
Source: American Journal of Respiratory Cell and Molecular Biology - October 15, 2013 Category: Molecular Biology Authors: Hudy MH, Traves SL, Proud D Tags: Am J Respir Cell Mol Biol Source Type: research

Late-breaking abstract: Inhibition of RC kinase is a novel therapeutic approach for the treatment of COPD and IPF
RC kinase is a novel serine/threonine kinase, expressed predominantly by pulmonary macrophage and type II bronchial epithelial cells. Examination of BAL macrophage mRNA isolated from COPD and IPF patients showed increased expression of RC kinase compared to normal smokers and non-smoking patients. In vitro studies showed an upregulation of RC kinase upon cell stress, including oxidative stress. This correlated with an increase of NF-kb activity and IL-8 production that could be inhibited by RC kinase siRNA or small molecule antagonists. Biochemical analysis revealed that RC kinase is predominantly a nuclear protein and int...
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Boehme, S., Coelho, A., Hogaboam, C., Ly, T. W., Bacon, K., Athanacio, J. Tags: 5.1 Airway Pharmacology and Treatment Source Type: research

p300 and C/EBP β-regulated IKKβ expression are involved in thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells
In this study, we aimed to investigate the roles of p300 and C/EBPβ-reliant IKKβ expression in thrombin-induced IL-8/CXCL8 expression. Thrombin-induced increases in IL-8/CXCL8-luciferase activity and IL-8/CXCL8 release were inhibited by p300 small interfering (siRNA). Thrombin-caused histone H3 acetylation was attenuated by p300 siRNA. Stimulation of cells with thrombin for 12h resulted in increases in IKKβ expression and phosphorylation in human lung epithelial cells. However, thrombin did not affect p65 expression. Moreover, 12h of thrombin stimulation produced increases in IKKβ expression and phosphorylation, and I...
Source: Pharmacological Research - April 26, 2017 Category: Drugs & Pharmacology Source Type: research