MiR-34a ameliorates arterial blood flow in rats with lower limb arteriosclerosis obliterans via Sirt1 signaling pathway
In this study, we investigated the impact of microRNA-34a (miR-34a) on lower limb arteriosclerosis obliterans in rats through the Sirtuin 1 (Sirt1) signaling pathway. Thirty-six Sprague-Dawley rats were divided into normal, model, and miR-34a mimics groups. Rats in the normal group were raised normally, while the model group underwent lower limb arteriosclerosis obliterans induction and received saline injections. The miR-34a mimics group also underwent arteriosclerosis obliterans modeling but received miR-34a mimics injections. Immunohistochemistry revealed significantly increased vascular endothelial growth factor (VEGF)...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Tuo Xu Changwei Zheng Yongkang Wu Zhengde Chen Huilai Miao Source Type: research
Importin 7 enhances protective autophagy induced by nuclear translocation of homeobox A10 in oral squamous cell carcinoma
This study aimed to explore the function and mechanism of IPO7 in OSCC. IPO7 expression in tissues and cells was determined by RT-qPCR. Cell proliferative, migratory, and invasive capabilities were detected through transwell assay and colony formation assay. Mice xenograft models were established for evaluating tumor growth. Autophagy was estimated by the LC3 levels in cells through western blot and immunofluorescence (IF). Western blot was utilized to detect the key proteins in PERK/EIF2AK3/ATF4 pathway for assessing the endoplasmic reticulum stress (ERS). The interaction of IPO7 and homeobox A10 (HOXA10) was tested by GS...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Shanshan Chen Jianbo Pan Source Type: research
The therapeutic effects of ADSCs on photoaging of skin induced by UVB irradiation
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):233-240. doi: 10.14715/cmb/2024.70.3.35.ABSTRACTSkin photoaging affects appearance and is associated with a variety of skin diseases, even skin cancer. Therefore, the prevention and treatment of skin photoaging is very important. However, there is a lack of effective evaluation methods, so it is an urgent problem to explore a comprehensive, non-invasive and in vivo evaluation method. Adipose-derived mesenchymal stem cells (ADSCs) are widely used to improve skin conditions as easier to obtain and positive effects. Recently, as the development of ultrasound technology, skin u...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jing Wang Xiaochen Zhou Di Cao Ying Meng Yingchun Wang Debin Yang Source Type: research
MiR-4454 in Combined Allergic Rhinitis and Asthma Syndrome (CARAS): Clinical significance and mechanistic insights into airway inflammation
This study found that inhibiting the expression of miR-4454 can improve HDM-induced cell injury, which may be related to miR-4454 regulating the activation of IL-17/NF-кB inflammatory axis.PMID:38650129 | DOI:10.14715/cmb/2024.70.3.34 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Zhiguang Liu Chuang Sun Yujia Shi Zhipeng Wang Lianzheng Zhou Qian Zhang Zhengdao Mao Source Type: research
Molecular mechanism of high glucose-induced mitochondrial DNA damage in retinal ganglion cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):219-224. doi: 10.14715/cmb/2024.70.3.33.ABSTRACTMitochondrial DNA damage in retinal ganglion cells (RGCs) may be closely related to lesions of glaucoma. RGCs were cultured with different concentrations of glucose and grouped into 3 groups, namely normal control (NC) group, Low-Glu group, and High-Glu group. Cell viability was measured with cell counting kit-8, and cell apoptosis was measured using flow cytometry. The DNA damage was measured with comet assay, and the morphological changes of damaged mitochondria in RGCs were observed using TEM. Western blot analyzed the expr...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jinzi Zhou Fenghua Chen Aimin Yan Xiaobo Xia Source Type: research
Research of the unrecognised functions of miR-375 in prostate cancer cells
In conclusion, miR-375 has anti-proliferative and cell migration inhibitory effects in prostate cancer. However, studies demonstrate that miR-375 may have tumor suppressor and oncogenic effects when considering cell molecular differences.PMID:38650131 | DOI:10.14715/cmb/2024.70.3.32 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Merve Goztepe Onur Eroglu Source Type: research
DNMT1 methylation of LncRNA-ANRIL causes myocardial fibrosis pyroptosis by interfering with the NLRP3/Caspase-1 pathway
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):197-203. doi: 10.14715/cmb/2024.70.3.30.ABSTRACTMyocardial fibrosis is a common pathological manifestation that occurs in various cardiac diseases. The present investigation aims to reveal how DNMT1/lncRNA-ANRIL/NLRP3 influences fibrosis and cardiac fibroblast pyroptosis. Here, we used ISO to induce myocardial fibrosis in mice, and LPS and ATP to induce myocardial fibroblast pyroptosis. The results showed that DNMT1, Caspase-1, and NLRP3 expression were significantly increased in fibrotic murine myocardium and pyroptotic cardiac fibroblasts, whereas LncRNA-ANRIL expression ...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Zuntao Liu Lei Gao Chenjing Kan Xin Chen Kaihu Shi Wei Wang Source Type: research
circ_JMJD1C expedites breast cancer progression by regulating miR-182-5p/JMJD1C/SOX4 axis
This study aimed to investigate the roles of circ_0006743 (circ_JMJD1C) in breast cancer. The downstream of circ_JMJD1C and their interaction network was determined by bioinformatic analyses. Gene expression were analyzed through western blot and qRT-PCR assays. Functional assays were conducted in vitro and in vivo to verify circ_JMJD1C role in BC. FISH and confocal analysis indicated the cellular distribution of circ_JMJD1C. Luciferase reporter, RNA immune-precipitation (RIP) assays, as well as Pearson's correlation analysis, were implemented to test the relation of miR-182-5p, JMJD1C and circ_JMJD1C. Circ_JMJD1C and JMJD...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Renyuan Xu Haomiao Lan Li Zhang Sisi Yang Yu Mao Hongying Che Source Type: research
Identification of important genes for human periodontal ligament cells in response to mechanical force based on WGCNA
In conclusion, we developed important genes for human PDLC in response to MF, which might be potential markers for orthodontic tooth movement.PMID:38650134 | DOI:10.14715/cmb/2024.70.3.29 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Xiaofeng Zhou Source Type: research
Diagnostic value of high-frequency ultrasound combined with fine needle aspiration cytology and BRAF gene for papillary thyroid microcarcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):187-191. doi: 10.14715/cmb/2024.70.3.28.ABSTRACTWe attempted to evaluate clinical application value of high-frequency ultrasound (HFUS), fine needle aspiration cytology (FNAC), BRAF gene, and combination of HFUS, FNAC, and BRAF gene in diagnosing papillary thyroid microcarcinoma (PTMC). The 150 patients with thyroid minimal lesions who underwent HFUS, FNAC and BRAF gene testing before surgery in our hospital from June 2020 to December 2021 were selected as research subjects. Patients were divided into two groups based on postoperative pathological results. The consistency o...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Chuang Li Xiaojuan Zhao Jingge Zhao Jing Zhao Lemei An Gang Wu Source Type: research
Small nucleolar RNA host gene 1 silence inhibits the lipopolysaccharide-induced microglial apoptosis and inflammation
This study was designed to investigate the role of small nucleolar RNA host gene 1 (SNHG1) on the apoptosis and inflammatory response of microglial cell BV-2 and its underlying molecular mechanism. The C5 lamina contusion-induced mouse model of spinal cord injury (SCI) was constructed. Mouse microglia BV2 was stimulated by lipopolysaccharide (LPS) to establish the in vitro model of SCI. The quantitative reverse transcription polymerase chain reaction method was used to quantify RNA expression levels. Enzyme-linked immunosorbent assays were used to quantify concentrations of inflammatory cytokines. Protein levels were asses...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hongtao Shen Jiaao Gu Xing Liu Mowei Song Source Type: research
"Simmer pus and grow flesh" method promotes chronic wound healing in rats via bFGF-Wnt β-catenin signaling pathway
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):155-161. doi: 10.14715/cmb/2024.70.3.23.ABSTRACTThe purpose of this study was to explore the mechanism of "simmer pus and grow meat" method based on bFGF regulating WNT / β-Catenin signaling pathway. Of 100 SPF rats, 25 were randomly selected as blank group, and 75 rats were established chronic infectious wound model and divided into blank group, model group (normal saline treatment, n = 25), experimental group (purple and white ointment treatment, n = 25), and wet burn ointment group (wet burn treatment, n = 25). The wound healing rate of rats was compared. The protein ex...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Zhenpeng Xu Erwei Cai Suyuan Shan Cheng Zhao Feng Lin Yanyan Wu Source Type: research
The influence of genetic predisposition to oxidative stress on painful diabetic peripheral neuropathy: A mendelian randomization study
This study employed a Mendelian Randomization (MR) approach to investigate the causal relationship between genetic predisposition to OS and PDPN. Genetic instruments associated with OS biomarkers were selected as exposures. Summary-level data on PDPN was obtained from the largest available genome-wide association study (GWAS). MR analyses were conducted using the inverse-variance weighted (IVW) method, with sensitivity analyses employing the MR-Egger, weighted median, and MR-PRESSO approaches. Genetic predisposition to increased glutathione S-transferase (GST) activity was associated with a reduced risk of PDPN (OR=0.66, 9...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Liyan Zou Duosheng Zhu Min Gong Jiangyi Yu Source Type: research
Knockdown of lncRNA AU020206 could inhibit microglia apoptosis in ischemic stroke
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):142-147. doi: 10.14715/cmb/2024.70.3.21.ABSTRACTThe diagnostic biomarkers associated with ischemic stroke (IS) that may have clinical utility remain elucidated. Thus, the potential functional lncRNAs in IS were explored. The Gene Expression Omnibus database provided the transcriptome profile of IS for download. WGCNA analysis and integrated bioinformatics were used to find genes that were differentially expressed (DEGs). The Starbase database created the lncRNA-based ceRNA network. In order to investigate the molecular mechanism and involved pathways of DEGs in IS, function...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Zhihua Chen Wei Hu Jiahong Wang Donggen Rao Jianming Zhu Source Type: research
Identification of DLEU2 as biomarker based on lncRNA-Mediated ceRNA network in rupture of intracranial aneurysm
In conclusion, a lncRNAs-mediated ceRNA network was constructed in IA based on the integrated bioinformatics analyses, in which DLEU2 was identified to be a novel and potential biomarker of IA.PMID:38650140 | DOI:10.14715/cmb/2024.70.3.22 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Yanlei Gao Tao Yu Source Type: research
