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Therapy: Gene Therapy

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Total 490 results found since Jan 2013.

Silencing of Phospholipase C gamma 2 Promotes Proliferation of Rat Hepatocytes In Vitro
In conclusion, these findings suggest that interference with PLCγ2 expression could relieve the inhibitory effect of PLCγ2 on hepaocyte apoptosis, thus promote proliferation through inactivating PKCδ‐mediated JNK1/2 signaling pathway. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Xiaoguang Chen, Xuemin Zhu, Yumei Liu, Qiongxia Lv, Jun Ma Tags: Article Source Type: research

A hyaluronan-based nanosystem enables combined anti-inflammation of mTOR gene silencing and pharmacotherapy
In this study, we synthesized a hyaluronan-based ROS-sensitive polymer which was expected to release loaded chemical drugs in inflammatory environment and further developed a stable and nontoxic co-delivery nanosystem of siRNA targeting autophagy suppressive gene and chemotherapeutic agents. The in vitro transfection study of this nanosystem revealed improved intracellular accumulation of siRNA and excellent gene silencing efficacy comparable to that of conventional cationic liposome. Moreover, the mRNA expression of inflammatory cytokines was remarkably decreased by our nanosystem. Considering its biocompatibility, transf...
Source: Carbohydrate Polymers - May 3, 2018 Category: Biomedical Science Source Type: research

Molecules, Vol. 23, Pages 1085: Liposomes as Gene Delivery Vectors for Human Placental Cells
In this study, we evaluated the potential of liposomes as carriers for small interfering RNAs to placental cells. Three neutral formulations carrying rhodamine-labelled siRNAs were evaluated on an in vitro model, i.e., human primary villous cytotrophoblasts. siRNA internalization rate from lipoplexes were compared to the one in the presence of the lipofectamine reagent and assessed by confocal microscopy. Results showed cellular internalization of nucleic acid with all three formulations, based on two cationic lipids, either DMAPAP or CSL-3. Moreover, incubation with DMAPAP+AA provided a rate of labelled cells as high as w...
Source: Molecules - May 4, 2018 Category: Chemistry Authors: Lucie Valero Khair Alhareth Jenifer Espinoza Romero Warren Viricel Jeanne Leblond Audrey Chissey H élène Dhotel Caroline Roques Danielle Campiol Arruda Virginie Escriou Nathalie Mignet Thierry Fournier Karine Andrieux Tags: Communication Source Type: research

A hyaluronan-based nanosystem enables combined anti-inflammation of mTOR gene silencing and pharmacotherapy.
In this study, we synthesized a hyaluronan-based ROS-sensitive polymer which was expected to release loaded chemical drugs in inflammatory environment and further developed a stable and nontoxic co-delivery nanosystem of siRNA targeting autophagy suppressive gene and chemotherapeutic agents. The in vitro transfection study of this nanosystem revealed improved intracellular accumulation of siRNA and excellent gene silencing efficacy comparable to that of conventional cationic liposome. Moreover, the mRNA expression of inflammatory cytokines was remarkably decreased by our nanosystem. Considering its biocompatibility, transf...
Source: Atherosclerosis - May 31, 2018 Category: Cardiology Authors: Hou C, Bai H, Wang Z, Qiu Y, Kong LL, Sun F, Wang D, Yin H, Zhang X, Mu H, Duan J Tags: Carbohydr Polym Source Type: research

Reversal of Multidrug Resistance in an Epirubicin-Resistant Gastric Cancer Cell Subline
Conclusions: ABCB1 knockdown decreased EPI efflux and increased EPI sensitivity in AGS/EPI cells. This result provides a novel strategy for targeted gene therapy to reverse EPI resistance in gastric cancer. PMID: 29801407 [PubMed - in process]
Source: Asian Pacific Journal of Cancer Prevention - May 31, 2018 Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

Correlation between S100A11 and the TGF- β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
Correlation between S100A11 and the TGF-β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1. Mol Cell Biochem. 2018 Jun 19;: Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Abstract S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β1/SMAD4/p21 pathway. S100A11 and TGF-β1 protein express...
Source: Molecular and Cellular Biochemistry - June 19, 2018 Category: Biochemistry Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Tags: Mol Cell Biochem Source Type: research

Destination Penis? Erectile Dysfunction As Possible Future Indication Of Therapeutic Gene Delivery.
Abstract Erectile Dysfunction (ED) is a common health problem in roughly 50% of males of advanced age (40-70 years old). Recent attention related gene therapy to ED cases; this received much interest to further progress gene therapy ideals to ED treatment. This review is an attempt to analyze key challenges and to emphasize primary areas, including mostly preclinical and few clinical trials, cellular target(s), and different viral vectors/nanoparticles for gene delivery in ED. While overexpression of target genes can be silenced by RNA interference (RNAi), down-regulation of these mechanisms has been implicated in...
Source: Current Gene Therapy - July 29, 2018 Category: Genetics & Stem Cells Authors: Gur S, Abdel-Mageed AB, Sikka SC, Bartolome AR, Hellstrom WJG Tags: Curr Gene Ther Source Type: research

Lentiviral ‑mediated inducible silencing of TLR4 attenuates neuropathic pain in a rat model of chronic constriction injury.
In conclusion, TLR4 may serve a significant role in neuropathy and the results of the present study provide an inducible lentivirus‑mediated siRNA against TLR4 that may serve as a potential novel strategy to be applied in gene therapy for NP in the future. PMID: 30365084 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - October 27, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases.
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

Otx2 enhances transdifferentiation of M üller cells-derived retinal stem cells into photoreceptor-like cells.
In conclusion, Otx2 enhances transdifferentiation of MC-RSCs into photoreceptor-like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target for gene therapy of retinal degenerative diseases. PMID: 30451368 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - November 19, 2018 Category: Molecular Biology Authors: Xiong Y, Ji H, You Z, Yao F, Zhou R, Song W, Xia X Tags: J Cell Mol Med Source Type: research

Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity.
In this study, we developed an advanced variant of the dendriplexes by encapsulating them within liposomes to enhance their gene delivery efficiency. This liposome encapsulated dendriplex system can further reduce unwanted cytotoxicity and enhance cellular uptake of nucleic acids. A broad range of lipid combinations were used to optimize the lipodendriplexes in terms of their physicochemical characteristics including size, shape and zeta potential. The optimized lipodendriplexes were tested for pDNA transfection, in vitro cell viability, cellular uptake, siRNA mediated knockdown, hemocompatibility, metastatic progression a...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 24, 2018 Category: Drugs & Pharmacology Authors: Tariq I, Reddy Pinnapireddy S, Duse L, Yasir Ali M, Ali S, Umair Amin M, Goergen N, Jedelská J, Schäfer J, Bakowsky U Tags: Eur J Pharm Biopharm Source Type: research

Autophagy inhibitor Vacuolin-1 interferes with lipid-based small interference RNA delivery.
Abstract Autophagy and endocytosis are important pathways regulating macromolecule recycling and regeneration. Small molecule inhibitors are utilized to modulate these pathways and to treat autophagy-related diseases. Vacuolin-1 is a small molecule that can potently and reversibly inhibit autophagy by activating Rab5. In addition, Vacuolin-1 can be applied to inhibit exocytosis in a variety of cell types. Here we report that Vacuolin-1 significantly reduces small interference RNA (siRNA)-mediated gene silencing delivered by liposome transfection reagent or lipid nanoparticles in Hela cells. Vacuolin-1 exhibits the...
Source: Biochemical and Biophysical Research communications - February 4, 2019 Category: Biochemistry Authors: Li T, Yue J, Huang L, Yang M Tags: Biochem Biophys Res Commun Source Type: research

A reactive oxygen species-responsive dendrimer with low cytotoxicity for efficient and targeted gene delivery
Publication date: Available online 25 March 2019Source: Chinese Chemical LettersAuthor(s): Yueqi Wang, Changjian Li, Libo Du, Yang LiuAbstractNonviral vectors have been attracting more attention for several advantages in gene delivery and the development of nonviral gene carriers with high delivery efficiency and low cytotoxicity has long been a key project. Starburst polyamidoamine dendrimers are a class of synthetic polymers with unique structural and physical characteristics. However, when they are used as gene carrier, the gene transfection efficiency is not satisfactory. Herein, a novel thioketal-core polyamidoamine d...
Source: Chinese Chemical Letters - March 26, 2019 Category: Chemistry Source Type: research

SOCS and Herpesviruses, With Emphasis on Cytomegalovirus Retinitis
Christine I. Alston1,2 and Richard D. Dix1,2* 1Department of Biology, Viral Immunology Center, Georgia State University, Atlanta, GA, United States 2Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA, United States Suppressor of cytokine signaling (SOCS) proteins provide selective negative feedback to prevent pathogeneses caused by overstimulation of the immune system. Of the eight known SOCS proteins, SOCS1 and SOCS3 are the best studied, and systemic deletion of either gene causes early lethality in mice. Many viruses, including herpesviruses such as herpes simplex virus and cytomega...
Source: Frontiers in Immunology - April 10, 2019 Category: Allergy & Immunology Source Type: research

RNA-based Therapy for Osteogenesis
Publication date: Available online 5 August 2019Source: International Journal of PharmaceuticsAuthor(s): Pinpin Wang, Federico Perche, Delphine Logeart-Avramoglou, Chantal PichonAbstractNucleic acid-based therapy has shown great promise in accelerating bone regeneration as well as other diseases. Nucleic acids used in gene therapy mainly are either plasmid DNA (pDNA) or RNAs. Although pDNA therapy has been extensively studied for decades with encouraging preclinical and clinical results, side effects, and low efficiency associated with nuclear trafficking are hard to bypass. Unlike pDNA, RNAs (mRNA, siRNA, miRNA) exert the...
Source: International Journal of Pharmaceutics - August 6, 2019 Category: Drugs & Pharmacology Source Type: research