Sphingosine is involved in PAPTP-induced death of pancreas cancer cells by interfering with mitochondrial functions
AbstractPancreas ductal adenocarcinoma belongs to the most common cancers, but also to the tumors with the poorest prognosis. Here, we pharmacologically targeted a mitochondrial potassium channel, namely mitochondrial Kv1.3, and investigated the role of sphingolipids and mutated Kirsten Rat Sarcoma Virus (KRAS) in Kv1.3-mediated cell death. We demonstrate that inhibition of Kv1.3 using the Kv1.3-inhibitor PAPTP results in an increase of sphingosine and superoxide in membranes and/or membranes associated with mitochondria, which is enhanced by KRAS mutation. The effect of PAPTP on sphingosine and mitochondrial superoxide fo...
Source: Journal of Molecular Medicine - May 23, 2024 Category: Molecular Biology Source Type: research
Dietary potassium loads call on intercalated cell PIEZO1 to go with the flow.
Adult humans in steady-state excrete ∼90% of dietary K+ via the kidney’s connecting segment and cortical collecting duct. Baseline renal K+ secretion is mediated by the principal cell (PC) ROMK K+ channel in a manner sustained by active ENaC-mediated, amiloride-sensitive Na+ reabsorption (Figure 1). 1,2 The transition to a high K+ (HiK) diet increases renal tubular flow rates, leading to an amiloride-insensitive increase in renal K+ secretion within less than a day. In rabbit kidney, amiloride-insensitive renal K+ excretion develops only after weaning. (Source: Kidney International)
Source: Kidney International - May 23, 2024 Category: Urology & Nephrology Authors: Seth L. Alper, Fouad T. Chebib Tags: nephrology digest Source Type: research
Identification of Potential Drug Targets of Calix[4]arene by Reverse Docking
Chemistry. 2024 May 22:e202400871. doi: 10.1002/chem.202400871. Online ahead of print.ABSTRACTCalixarenes are displaying great potential for the development of new drug delivery systems, diagnostic imaging, biosensing devices and inhibitors of biological processes. In particular, calixarene derivatives are able to interact with many different enzymes and function as inhibitors. By screening of the potential drug target database (PDTD) with a reverse docking procedure, we identify and discuss a selection of 100 proteins that interact strongly with calix[4]arene. We also discover that leucine (23.5%), isoleucine (11.3%), phe...
Source: Chemistry - May 22, 2024 Category: Chemistry Authors: Giulia Giugliano Margherita Gajo Tainah Dorina Marforio Francesco Zerbetto Edoardo Jun Mattioli Matteo Calvaresi Source Type: research
Identification of Potential Drug Targets of Calix[4]arene by Reverse Docking
Chemistry. 2024 May 22:e202400871. doi: 10.1002/chem.202400871. Online ahead of print.ABSTRACTCalixarenes are displaying great potential for the development of new drug delivery systems, diagnostic imaging, biosensing devices and inhibitors of biological processes. In particular, calixarene derivatives are able to interact with many different enzymes and function as inhibitors. By screening of the potential drug target database (PDTD) with a reverse docking procedure, we identify and discuss a selection of 100 proteins that interact strongly with calix[4]arene. We also discover that leucine (23.5%), isoleucine (11.3%), phe...
Source: Chemistry - May 22, 2024 Category: Chemistry Authors: Giulia Giugliano Margherita Gajo Tainah Dorina Marforio Francesco Zerbetto Edoardo Jun Mattioli Matteo Calvaresi Source Type: research
MnFe Prussian Blue Analogue Open Cages for Sodium ‐Ion Batteries: Simultaneous Evolution of Structure, Morphology, and Energy Storage Properties
This study offers valuable insights into the intricate relationship between the structure, morphology, and electrochemical pe rformance of MnFe PBA, paving the way for further optimizations in this promising class of materials for energy storage applications. (Source: Small)
Source: Small - May 22, 2024 Category: Nanotechnology Authors: Weilu Wang,
Zheng Xing,
Haipeng Ren,
Qinglin Wang,
Xinran Gao,
Chuanhao Nie,
Zhicheng Ju Tags: Research Article Source Type: research
Molecules, Vol. 29, Pages 2437: Structure & ndash;Activity Relationship Studies in a Series of Xanthine Inhibitors of SLACK Potassium Channels
Molecules, Vol. 29, Pages 2437: Structure–Activity Relationship Studies in a Series of Xanthine Inhibitors of SLACK Potassium Channels
Molecules doi: 10.3390/molecules29112437
Authors:
Alshaima’a M. Qunies
Brittany D. Spitznagel
Yu Du
Paul K. Peprah
Yasmeen K. Mohamed
C. David Weaver
Kyle A. Emmitte
Gain-of-function mutations in the KCNT1 gene, which encodes the sodium-activated potassium channel known as SLACK, are associated with the rare but devastating developmental and epileptic encephalopathy known as epilepsy of infancy with migrating focal seizures (EIMFS). The design of small molecu...
Source: Molecules - May 22, 2024 Category: Chemistry Authors: Alshaima ’a M. Qunies Brittany D. Spitznagel Yu Du Paul K. Peprah Yasmeen K. Mohamed C. David Weaver Kyle A. Emmitte Tags: Article Source Type: research
K+ selectivity modulation in non-aqueous CO2 electroreduction on lead catalyst: from oxalic to tartaric acid production
J. Mater. Chem. A, 2024, Accepted Manuscript DOI: 10.1039/D4TA01172D, PaperEduardo Arizono dos Reis, Gelson T. S. T. Silva, Caue Ribeiro Here, we show that the presence of potassium ions in the catholyte modulates the selectivity of the Pb plate electrode, leading to the formation of tartrate, a C4 compound, from... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - J. Mater. Chem. latest articles)
Source: RSC - J. Mater. Chem. latest articles - May 21, 2024 Category: Chemistry Authors: Eduardo Arizono dos Reis Source Type: research
Insertion sequence IS6770 modulates potassium symporter kup transcription in Enterococcus faecalis JH2-2 under low pH conditions
In this study, we observed that the kup gene promoter region in the JH2-2 strain was modified due to the insertion of a complete copy of the IS6770 insertion sequence. Consequently, we investigated the influence of IS6770 on the expression of the kup gene. To achieve this, we conducted a mapping of the promoter region of this gene in the E. faecalis JH2-2 strain, employing fluorescence gene reporters. In addition, a transcriptional analysis of the kup gene was executed in a strain derived from E. faecalis V583 that lacks the IS30-related insertion element, facilitating the identification of the transcriptional start site. ...
Source: International Journal of Food Microbiology - May 21, 2024 Category: Food Science Authors: Giuliana Acciarri Maria Eugenia Taborra Fernan O Gizzi Victor S Blancato Christian Magni Source Type: research
Widespread use of proton pump inhibitors or potassium-competitive acid blocker has changed the status of gastrointestinal bleeding in patients with ischemic heart disease: real-world data from high volume centers
Although proton pump inhibitors (PPIs) or potassium-competitive acid blocker (PCAB) are useful in peptic ulcer prevention, their efficacy in preventing other gastrointestinal bleeding remains unclear. This stu... (Source: BMC Gastroenterology)
Source: BMC Gastroenterology - May 21, 2024 Category: Gastroenterology Authors: Shun Sasaki, Kazuhiro Ota, Makoto Sanomura, Yosuke Mori, Hironori Tanaka, Akitoshi Hakoda, Noriaki Sugawara, Taro Iwatsubo, Yuki Hirata, Kazuki Kakimoto, Hideaki Morita, Wataru Nagamatsu, Masaaki Hoshiga, Toshihisa Takeuchi, Kazuhide Higuchi and Hiroki Ni Tags: Research Source Type: research
Characterizing the cellular and molecular landscape of mineralocorticoid action and antagonism in the kidney
The mineralocorticoid hormone aldosterone plays a major role in sodium and potassium homeostasis and blood volume regulation. Aldosterone binds to the mineralocorticoid receptor (MR) in the cytosol, and this complex translocates into the nucleus and binds to DNA, regulating target gene transcription. Although cortisol can bind to the MR as well, in mineralocorticoid-sensitive tissues, the enzyme 11 β-hydroxysteroid dehydrogenase type 2 degrades glucocorticoids and MR is exclusively activated by mineralocorticoids. (Source: Kidney International)
Source: Kidney International - May 20, 2024 Category: Urology & Nephrology Authors: Ver ónica Miguel, Tilman B. Drueke Tags: Nephrology Digest Source Type: research
Protective effect of magnesium preloading on cisplatin-induced nephrotoxicity: which dose is more suitable?
CONCLUSIONS: We clearly demonstrated that saline hydration with 24 mEql MgSO4 supplementation before cisplatin treatment has a better renal protective effect than 12 mEql MgSO4 without reducing efficacy, especially in patients with local/local advanced cervical and head-neck cancer receiving cisplatin with concurrent radiotherapy.PMID:38766796 | DOI:10.26355/eurrev_202405_36185 (Source: Pharmacological Reviews)
Source: Pharmacological Reviews - May 20, 2024 Category: Drugs & Pharmacology Authors: Y Ilhan A H Onder M F Ozbay G Karakaya S Sezgin Goksu B Ozturk H S Coskun Source Type: research
Protective effect of magnesium preloading on cisplatin-induced nephrotoxicity: which dose is more suitable?
CONCLUSIONS: We clearly demonstrated that saline hydration with 24 mEql MgSO4 supplementation before cisplatin treatment has a better renal protective effect than 12 mEql MgSO4 without reducing efficacy, especially in patients with local/local advanced cervical and head-neck cancer receiving cisplatin with concurrent radiotherapy.PMID:38766796 | DOI:10.26355/eurrev_202405_36185 (Source: Cancer Control)
Source: Cancer Control - May 20, 2024 Category: Cancer & Oncology Authors: Y Ilhan A H Onder M F Ozbay G Karakaya S Sezgin Goksu B Ozturk H S Coskun Source Type: research
Protective effect of magnesium preloading on cisplatin-induced nephrotoxicity: which dose is more suitable?
CONCLUSIONS: We clearly demonstrated that saline hydration with 24 mEql MgSO4 supplementation before cisplatin treatment has a better renal protective effect than 12 mEql MgSO4 without reducing efficacy, especially in patients with local/local advanced cervical and head-neck cancer receiving cisplatin with concurrent radiotherapy.PMID:38766796 | DOI:10.26355/eurrev_202405_36185 (Source: European Review for Medical and Pharmacological Sciences)
Source: European Review for Medical and Pharmacological Sciences - May 20, 2024 Category: Drugs & Pharmacology Authors: Y Ilhan A H Onder M F Ozbay G Karakaya S Sezgin Goksu B Ozturk H S Coskun Source Type: research
Protective effect of magnesium preloading on cisplatin-induced nephrotoxicity: which dose is more suitable?
CONCLUSIONS: We clearly demonstrated that saline hydration with 24 mEql MgSO4 supplementation before cisplatin treatment has a better renal protective effect than 12 mEql MgSO4 without reducing efficacy, especially in patients with local/local advanced cervical and head-neck cancer receiving cisplatin with concurrent radiotherapy.PMID:38766796 | DOI:10.26355/eurrev_202405_36185 (Source: Pharmacological Reviews)
Source: Pharmacological Reviews - May 20, 2024 Category: Drugs & Pharmacology Authors: Y Ilhan A H Onder M F Ozbay G Karakaya S Sezgin Goksu B Ozturk H S Coskun Source Type: research
Protective effect of magnesium preloading on cisplatin-induced nephrotoxicity: which dose is more suitable?
CONCLUSIONS: We clearly demonstrated that saline hydration with 24 mEql MgSO4 supplementation before cisplatin treatment has a better renal protective effect than 12 mEql MgSO4 without reducing efficacy, especially in patients with local/local advanced cervical and head-neck cancer receiving cisplatin with concurrent radiotherapy.PMID:38766796 | DOI:10.26355/eurrev_202405_36185 (Source: European Review for Medical and Pharmacological Sciences)
Source: European Review for Medical and Pharmacological Sciences - May 20, 2024 Category: Drugs & Pharmacology Authors: Y Ilhan A H Onder M F Ozbay G Karakaya S Sezgin Goksu B Ozturk H S Coskun Source Type: research
