Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Innovative treatment of industrial effluents through combining ferric iron and attapulgite application
Chemosphere. 2024 Apr 24;358:142132. doi: 10.1016/j.chemosphere.2024.142132. Online ahead of print.ABSTRACTThe escalation of industrial activities has escalated the production of pharmaceutical and dyeing effluents, raising significant environmental issues. In this investigation, a hybrid approach of Fenton-like reactions and adsorption was used for deep treatment of these effluents, focusing on effects of variables like hydrogen peroxide concentration, catalyst type, pH, reaction duration, temperature, and adsorbent quantity on treatment effectiveness, and the efficacy of acid-modified attapulgite (AMATP) and ferric iron ...
Source: Chemosphere - April 26, 2024 Category: Chemistry Authors: Zhenxiang Pan Bizhen Zeng Liguo Shen Jiaheng Teng Tongli Lai Leihong Zhao Genying Yu Hongjun Lin Source Type: research
Nutrient remobilization and C:N:P stoichiometry in response to elevated CO < sub > 2 < /sub > and low phosphorus availability in rice cultivars introgressed with and without Pup1
Plant Physiol Biochem. 2024 Apr 23;210:108657. doi: 10.1016/j.plaphy.2024.108657. Online ahead of print.ABSTRACTThe continuously rising atmospheric CO2 concentration potentially increase plant growth through stimulating C metabolism; however, plant C:N:P stoichiometry in response to elevated CO2 (eCO2) under low P stress remains largely unknown. We investigated the combined effect of eCO2 and low phosphorus on growth, yield, C:N:P stoichiometry, and remobilization in rice cv. Kasalath (aus type), IR64 (a mega rice variety), and IR64-Pup1 (Pup1 QTL introgressed IR64). In response to eCO2 and low P, the C accumulation increa...
Source: Physiological Research - April 26, 2024 Category: Physiology Authors: Sandeep Sharma D H Raviteja Tarun Kumar Prem S Bindraban Renu Pandey Source Type: research
Relationship between arginine methylation and vascular calcification
Cell Signal. 2024 Apr 24:111189. doi: 10.1016/j.cellsig.2024.111189. Online ahead of print.ABSTRACTIn patients on maintenance hemodialysis (MHD), vascular calcification (VC) is an independent predictor of cardiovascular disease (CVD), which is the primary cause of death in chronic kidney disease (CKD). The main component of VC in CKD is the vascular smooth muscle cells (VSMCs). VC is an ordered, dynamic activity. Under the stresses of oxidative stress and calcium‑phosphorus imbalance, VSMCs undergo osteogenic phenotypic transdifferentiation, which promotes the formation of VC. In addition to traditional epigenetics like ...
Source: Cellular Signalling - April 26, 2024 Category: Cytology Authors: Chen Chen Yuanyuan Ding Qun Huang Chen Zhang Zixia Zhao Hua Zhou Detian Li Guangyu Zhou Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Nutrient remobilization and C:N:P stoichiometry in response to elevated CO < sub > 2 < /sub > and low phosphorus availability in rice cultivars introgressed with and without Pup1
Plant Physiol Biochem. 2024 Apr 23;210:108657. doi: 10.1016/j.plaphy.2024.108657. Online ahead of print.ABSTRACTThe continuously rising atmospheric CO2 concentration potentially increase plant growth through stimulating C metabolism; however, plant C:N:P stoichiometry in response to elevated CO2 (eCO2) under low P stress remains largely unknown. We investigated the combined effect of eCO2 and low phosphorus on growth, yield, C:N:P stoichiometry, and remobilization in rice cv. Kasalath (aus type), IR64 (a mega rice variety), and IR64-Pup1 (Pup1 QTL introgressed IR64). In response to eCO2 and low P, the C accumulation increa...
Source: Physiological Research - April 26, 2024 Category: Physiology Authors: Sandeep Sharma D H Raviteja Tarun Kumar Prem S Bindraban Renu Pandey Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Molecules, Vol. 29, Pages 1988: Uniform P-Doped MnMoO4 Nanosheets for Enhanced Asymmetric Supercapacitors Performance
In this study, MnMoO4&middot;H2O nanosheets were synthesized on nickel foam via a hydrothermal route, and the MnMoO4&middot;H2O nanosheet structure was successfully doped with a phosphorus element using a gas&ndash;solid reaction method. Phosphorus element doping forms phosphorus&ndash;metal bonds and oxygen vacancies, thereby increasing the charge storage and conductivity of the electrode material. The specific capacitance value is as high as 2.112 F cm&minus;2 (1760 F g&minus;1) at 1 mA cm&minus;2, which is 3.2 times higher than that of the MnMoO4&middot;H2O...
Source: Molecules - April 26, 2024 Category: Chemistry Authors: Yu Liu Yan Li Zhuohao Liu Tao Feng Huichuan Lin Gang Li Kaiying Wang Tags: Article Source Type: research
Clinical Efficacy of Bisphosphonates in Treating Osteoporosis in
Diabetes Patients: A Meta-Analysis
Horm Metab Res DOI: 10.1055/a-2295-9335The aim of the study was to explore the clinical efficacy of bisphosphonates in
patients with osteoporosis in diabetes patients by meta-analysis. Six databases
were systematically searched from inception to January 30,2023. Studies
evaluating the treatment of diabetic osteoporosis with bisphosphonates were
included. Key outcome measures, such as bone mineral density (BMD), bone
metabolism markers, pain improvement, and safety assessments, were extracted and
analyzed. STATA MP V17.0 was used to calculate the combined effect si...
Source: Hormone and Metabolic Research - April 26, 2024 Category: Endocrinology Authors: Yang, Yuan-Xun Jin, Yan Tags: Original Article: Endocrine Care Source Type: research
Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research
Chlorpyrifos degradation and its impacts on phosphorus bioavailability in microplastic-contaminated soil
This study explores the degradation pathways of CPF (6 mg kg-1, 12 mg kg-1 of CPF addition) in soils with different levels of polylactic acid MPs (PLA-MPs) (0.0 %, 0.1 %, 0.5 %, 1.0 % w/w), and analyzes soil P fractions and phosphatase enzyme activities to investigate soil P bioavailability under different treatments. Results show that the degradation of CPF fits to a first-order decay model, with half-lives (DT50) ranging from 11.0 to 14.8 d depending on PLA-MPs treatment. The concentration of its metabolite 3, 5, 6-trichloropyridine 2-phenol (TCP) reached a peak of 0.93-1.67 mg kg-1 within 7-14 days. Similarly, the degra...
Source: Ecotoxicology and Environmental Safety - April 25, 2024 Category: Environmental Health Authors: Lingling Ding Yan Wang Hui Ju Darrell W S Tang Sha Xue Violette Geissen Xiaomei Yang Source Type: research
Phosphate Recovery from Urban Sewage by the Biofilm Sequencing Batch Reactor Process: Key Factors in Biofilm Formation and Related Mechanisms
Environ Res. 2024 Apr 23:118985. doi: 10.1016/j.envres.2024.118985. Online ahead of print.ABSTRACTThe biofilm sequencing batch reactor (BSBR) technique has been deployed in the laboratory to enrich phosphorus from simulated wastewater, but it is still not clear what its performance will be when real world sewage is used. In this work, the effluent from the multi-stage anoxic-oxic (AO) activated sludge process at a sewage plant was used as the feed water for a BSBR pilot system, which had three reactors operating at different levels of dissolved oxygen (DO). The phosphorus adsorption and release, the biofilm growth, and the...
Source: Environmental Research - April 25, 2024 Category: Environmental Health Authors: Chao Zheng Jun Zhang Min Ni Yang Pan Source Type: research
Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | PMC:PMC11045571 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research
