Epoxyeicosanoids in hypertension.
Abstract Epoxyeicosatrienoic acid (EETs) are also known as epoxyeicosanoids that have renal and cardiovascular actions. These renal and cardiovascular actions can be regulated by soluble epoxide hydrolase (sEH) that degrades and inactivates EETs. Extensive animal hypertension studies have determined that EET vascular, epithelial transport, and anti-inflammatory actions lower blood pressure and decrease renal and cardiovascular disease progression. Human studies have also supported the notion that increasing EET levels in hypertension could be beneficial. Pharmacological and genetic approaches to increase epoxyeico...
Source: Physiological Research - September 2, 2019 Category: Physiology Authors: Imig JD Tags: Physiol Res Source Type: research

The protective effect of resveratrol against cyclosporine A-induced oxidative stress and hepatotoxicity.
This study aimed to investigate the effect of resveratrol (RES) on CsA-induced oxidative stress and hepatotoxicity in rats. Rats were treated with RES (10 mg/kg/day; i.p.) for 14 days. CsA (25 mg/kg/day; s.c.) was given during the last seven days together with RES. Serum alanine aminotransferase and aspartate aminotransferase activities together with hepatic histopathological examinations were performed. ROS, thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPPs), ferric reducing antioxidant power, and glutathione levels as well as superoxide dismutase, and glut...
Source: Physiological Research - September 2, 2019 Category: Physiology Authors: Bingul I, Olgac V, Bekpinar S, Uysal M Tags: Arch Physiol Biochem Source Type: research

Uric acid level is positively associated with NT-proBNP concentration in Slovak heart failure patients.
Abstract Increased concentration of uric acid (UA) is positively associated with the clinical severity but negatively associated with the prognosis of heart failure (HF). However, data related to the association between UA concentration and N-terminal pro brain natriuretic peptide (NT-proBNP) are still lacking. The aim of the study was to analyze the relationships between UA, NT-proBNP, clearance of creatinine and NYHA function class and echocardiographic variables in the Slovak population of primary care patients diagnosed with HF. The association between UA and NT-proBNP was assessed by multivariate analysis. 84...
Source: Physiological Research - August 23, 2019 Category: Physiology Authors: Sabaka P, Dukat A, Gajdosik J, Caprnda M, Bendzala M, Simko F Tags: Physiol Res Source Type: research

Increased intestinal permeability in patients with short bowel syndrome is not affected by parenteral nutrition.
iacute;žová J, Trachta P, Mráz M, Haluzík M Abstract The aim of our study was to assess the presence and degree of intestinal leakage in subjects suffering from short bowel syndrome (SBS) and its modification by parenteral nutrition. To this end we assessed circulating levels of selected makers of intestinal permeability including zonulin, fatty acid binding protein 2 (FABP-2), citrulline and glucagon-like peptide 2 (GLP-2). We also measured lipopolysaccharide binding protein (LBP) as a marker of circulating levels of lipopolysaccharide acting through the CD14 molecule. Eleven SBS and 10 age-...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Cinkajzlová A, Lacinová Z, Kloučková J, Kaválková P, Kratochvílová H, Křížová J, Trachta P, Mráz M, Haluzík M Tags: Physiol Res Source Type: research

2Hz-electroacupuncture attenuates heroin-seeking behaviors via adjusts CB1-Rs and CB2-Rs expression in relapse-relevant brain regions of heroin self-administration rats.
Abstract Opiate addiction has a high rate of relapse. The accumulating evidence shows that electroacupuncture (EA) may be effective for the treatment of opiate relapse. However, the change of expression of CB1-Rs and CB2-Rs involve in 2Hz EA anti-relapse pathway is still unclear. To explore the changes of expression of CB1-Rs and CB2-Rs, heroin self-administration (SA) model rats were adopted and treated using 2Hz EA. The expressions of CB1-Rs and CB2-Rs were observed using immunohistochemistry method. The results showed that, compared with the control group, active pokes in the heroin-addicted group increased, wh...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Chen L, Gong XK, Leng CL, Ma BM, Ru Q, Xiong Q, Yue K, Zhou M, Tian X, Li CY, Wu YX Tags: Physiol Res Source Type: research

Biophysical and microstructural changes of swelling cornea caused by endothelial cells damage.
Abstract Biophysical properties and microstructural changes of swelling cornea which caused by endothelial cells damage will be evaluated. Swelling cornea models were established by endothelial cells damage in 114 Sprague Dawley rats. Relative gray value, swelling rate and light transmittance were measured to evaluated the biophysical properties and microstructure changes were observed by transmission electron microscopy. Relative gray value decreased while swelling rate rose along with time and both of them reached relative stability after 7 days. Light transmittance showed a decline trend with time even after co...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: He F, Liu J, Ye P, Wu J, Liang G, Xu W Tags: Physiol Res Source Type: research

Intradialytic hypotension: beyond hemodynamics.
In conclusion, endothelial dysfunction characterized by a lower level of vasoactive molecule seems to play a critical role in intradialytic hypotension development. PMID: 31424249 [PubMed - as supplied by publisher] (Source: Physiological Research)
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Hajal J, Joubran N, Sleilaty G, Chacra D, Saliba Y, Assaad S, Chelala D, Fares N Tags: Physiol Res Source Type: research

Intracerebroventricular endothelin receptor-A blockade in rats decreases phase-II ventricular tachyarrhythmias during acute myocardial infarction.
We examined ventricular tachyarrhythmias after endothelin receptor-A blockade in the brain of Wistar rats with acute myocardial infarction. For this aim, BQ-123 (n=6) or phosphate-buffered saline (n=6) were injected intracerebroventricularly. After 10 min, the left coronary artery was ligated, followed by implantation of telemetry transmitters. Electrocardiography and voluntary activity (as a surrogate of acute left ventricular failure) were continuously monitored for 24 h. Infarct-size was similar in the two groups. There were fewer episodes of ventricular tachyarrhythmias of shorter average duration in treated rats, lead...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Lekkas P, Georgiou ES, Kontonika M, Mouxtouri ET, Mourouzis I, Pantos K, Kolettis TM Tags: Physiol Res Source Type: research

Opioid and endocannabinoid system in orofacial pain.
Abstract Orofacial pain disorders are frequent in the general population and their pharmacological treatment is difficult and controversial. Therefore, the search for novel, safe and efficient analgesics is an important but still elusive goal for contemporary medicine. In the recent years, the antinociceptive potential of endocannabinoids and opioids has been emphasized. However, concerns for the safety of their use limit their clinical applications. the possibility of modulating the activity of endocannabinoids by regulation of their synthesis and/or degradation offers an innovative approach to the treatment of p...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Zubrzycki M, Stasiolek M, Zubrzycka M Tags: Physiol Res Source Type: research

Urine composition in pigs is regulated in both the ureter and the bladder.
Abstract The former perception of the urothelium as an impermeable barrier has been revised during the last decade, as increasing evidence of changes in urine composition during its passage of the urinary tract has been presented. Since differences in urothelial permeability between upper and lower urinary tract have been found, our aim is to demonstrate whether changes in urine composition occur during passage through the ureter. We studied consecutive urine samples from both renal pelvises in six pigs and compared them to samples from the bladder and distal ureter. We further sampled urine during storage in the ...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Jakobsen LK, Andersson KE, Norregaard R, Olsen LH Tags: Physiol Res Source Type: research

Strong coincidence between slow wave sleep and low AHI is explainable by the high instability of slow wave sleep to obstructive apnea exposure.
á A, Doničová V, Rusnáková S, Tomori Z, Donič V Abstract It is well known that in patients with obstructive sleep apnea syndrome (OSAS) the apnea-hypopnea index (AHI) is significantly decreased during slow wave sleep (SWS). It used to be explained by the ability of SWS to stabilize the upper airways against collapse. Another explanation, which is the focus of the current study, is that it is just a result of high instability of SWS to obstructive apnea exposure, i.e. high susceptibility of SWS to transition into lighter sleep stages during exposure to obstructive apneas. A retrospecti...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Peregrim I, Grešová S, Štimmelová J, Bačová I, Fulton BL, Tokárová D, Gáborová M, Brandeburová A, Doničová V, Rusnáková S, Tomori Z, Donič V Tags: Physiol Res Source Type: research

Distinct effects of bosentan on NO-dependent vasodilation and calcium influx in heterozygous Ren-2 transgenic rats on high-salt diet.
acute; M, Zicha J Abstract Our studies in hypertensive Ren-2 transgenic rats (TGR) demonstrated that chronic administration of atrasentan (ETA receptor antagonist) decreased blood pressure by reduced Ca(2+) influx through L-type voltage-dependent calcium channels (L-VDCC) and attenuated angiotensin II-dependent vasoconstriction. We were interested whether bosentan (nonselective ET(A)/ET(B) receptor antagonist) would have similar effects. Young 4-week-old (preventive study) and adult 8-week-old (regression study) heterozygous TGR and their normotensive Hannover Sprague-Dawley (HanSD) controls were fed normal-salt (...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Vaněčková I, Hojná S, Kadlecová M, Zicha J Tags: Physiol Res Source Type: research

Histone deacetylase inhibition has cardiac and vascular protective effects in rats with pressure overload cardiac hypertrophy.
Abstract Histone deacetylase (HDAC) inhibitors have shown beneficial effects in animal models of cardiovascular diseases. We hypothesized that HDAC inhibitor, sodium valproate (VPA), has cardiac and vascular protective effects in rats with pressure overload cardiac hypertrophy induced by transverse aortic constriction (TAC). Sections of the heart were visualized after hematoxylin and eosin staining, picrosirius red staining and immunohistochemistry. The expression of genes related to cardiac hypertrophy, fibrosis, and oxidative stress was determined by quantitative real-time polymerase chain reaction. The aortic r...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Jung H, Lee E, Kim I, Song JH, Kim GJ Tags: Physiol Res Source Type: research

Protective effect and mechanism of alpha-lipoic acid on partial hepatic ischemia-reperfusion injury in adult male rats.
Abstract In order to reduce tissue damage caused by ischemia-reperfusion injury, this study aims to investigate the protective effect and mechanism of alpha-lipoic acid on hepatic ischemia-reperfusion injury in rats. The bloodstream of rats was blocked in the left middle and left lateral liver lobes of the liver. Forty rats were randomly divided into two groups: treatment group and injury group. Rats were injected with either 25 mg/1 ml of alpha-lipoic acid (treatment group) or 1 ml of saline (injury group) into the caudal vein 15 min before hepatic ischemia-reperfusion. Rat serum alanine aminotransferase (GPT), g...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Ren Y, Wang LH, Deng FS, Li JS, Jiang L Tags: Physiol Res Source Type: research

Four weeks of hypoxia training improves cutaneous microcirculation in trained rowers.
Abstract Hypoxia training can improve endurance performance. However, the specific benefits mechanism of hypoxia training is controversial, and there are just a few studies on the peripheral adaptation to hypoxia training. The main objective of this study was to observe the effects of hypoxia training on cutaneous blood flow (CBF), hypoxia-inducible factor (HIF), nitric oxide (NO), and vascular endothelial growth factor (VEGF). Twenty rowers were divided into two groups for four weeks of training, either hypoxia training (Living High, Exercise High and Training Low, HHL) or normoxia training (NOM). We tested cutan...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Meng Z, Gao B, Gao H, Ge P, Li T, Wang Y Tags: Physiol Res Source Type: research

Long-term peritoneal dialysis treatment provokes activation of genes related to adaptive immunity.
Abstract Permanent irritation of the peritoneum during peritoneal dialysis (PD) treatment leads to local chronic inflammation and subsequently activation of processes driving fibrogenesis in the long-term. The aim of the study was to compare the peritoneal effluent transcriptome of 20 patients treated less and 13 patients treated more than 2 years using microarray analysis. An increased expression of genes associated with an immune response was observed in long-term treated patients with well preserved peritoneal function, when compared to patients treated less than 2 years. From 100 genes highly expressed in long...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Parikova A, Hruba P, Krediet RT, Krejcik Z, Stranecky V, Striz I, Viklicky O Tags: Physiol Res Source Type: research

Insulin-like growth factor axis in pregnancy and gestational diabetes mellitus.
; P, Krejčí H, Mráz M, Pařízek A, Haluzík M, Kršek M Abstract The insulin-like growth factor (IGF) is involved in the regulation of growth and metabolism. The aim of this study was to determine selected parameters of IGF system at systemic and local levels [subcutaneous (SAT) and visceral adipose tissue (VAT)] to assess its possible role in gestational diabetes mellitus (GDM). 37 pregnant women (21 with GDM and 16 without GDM) and 15 age-matched non-pregnant females were included in the study. Blood samples were taken in 28-32 and 36-38 weeks of gestation and 6-12 months after ...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Anderlová K, Cinkajzlová A, Šimják P, Kloučková J, Kratochvílová H, Lacinová Z, Kaválková P, Krejčí H, Mráz M, Pařízek A, Haluzík M, Kršek M Tags: Physiol Res Source Type: research

Transcriptomic analysis of left ventricle myocardium in an SHR congenic line with ameliorated cardiac fibrosis.
n;eda O, Liška F Abstract Metabolic syndrome and one of its manifestations, essential hypertension, is an important cause of worldwide morbidity and mortality. Morbidity and mortality associated with hypertension are caused by organ complications. Previously we revealed a decrease of blood pressure and an amelioration of cardiac fibrosis in a congenic line of spontaneously hypertensive rats (SHR), in which a short segment of chromosome 8 (encompassing only 7 genes) was exchanged for a segment of normotensive polydactylous (PD) origin. To unravel the genetic background of this phenotype we compared heart tra...
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Mirchi LF, Chylíková B, Janků M, Šeda O, Liška F Tags: Physiol Res Source Type: research

Inhibition of TRPA1 and IL-6 signal alleviates neuropathic pain following chemotherapeutic bortezomib.
In conclusion, we revealed specific signaling pathways leading to neuropathic pain induced by chemotherapeutic BTZ, including IL-6-TRPA1, suggesting that blocking these signals is beneficial to alleviate neuropathic pain during BTZ intervention. PMID: 31424261 [PubMed - as supplied by publisher] (Source: Physiological Research)
Source: Physiological Research - August 19, 2019 Category: Physiology Authors: Liu D, Sun M, Xu D, Ma X, Gao D, Yu H Tags: Physiol Res Source Type: research

The effects of dietary nitrate on plasma glucose and insulin sensitivity in sheep.
Abstract Nitrate (NO3 ¯ ) is an effective non-protein nitrogen source for gut microbes and reduces enteric methane (CH4 ) production in ruminants. Nitrate is reduced to ammonia by rumen bacteria with nitrite (NO2 ¯ ) produced as an intermediate. The absorption of NO2 ¯ can cause methaemoglobinaemia in ruminants. Metabolism of NO3 ¯ and NO2 ¯ in blood and animal tissues forms nitric oxide (NO) which has profound physiological effects in ruminants and has been shown to increase glucose uptake and insulin secretion in rodents and humans. We hypothesized that absorption of small quantities of ...
Source: Physiological Research - August 16, 2019 Category: Physiology Authors: Villar ML, Godwin IR, Hegarty RS, Dobos RC, Smith KA, Clay JW, Nolan JV Tags: J Anim Physiol Anim Nutr (Berl) Source Type: research

GFP Transgenic Animals in Biomedical Research: A Review of Potential Disadvantages.
ipi L Abstract Green Fluorescent protein (GFP) transgenic animals are accepted tools for studying various physiological processes, including organ development and cell migration. However, several in vivo studies claimed that GFP may impair transgenic animals' health. Glomerulosclerosis was observed in transgenic mice and rabbits with ubiquitous reporter protein expression. Heart-specific GFP expression evoked dilated cardiomyopathy and altered cardiac function in transgenic mouse and zebrafish lines, respectively. Moreover, growth retardation and increased axon swelling were observed in GFP and yellow fluorescent ...
Source: Physiological Research - July 25, 2019 Category: Physiology Authors: Lipták N, Bősze Z, Hiripi L Tags: Physiol Res Source Type: research

Assessment of the Effective Impact of Bisphenols on Mitochondrial Activity and Steroidogenesis in a Dose-Dependency in Mice TM3 Leydig Cells.
Abstract reproductive disorders in both sexes. The aim of the present in vitro study was to evaluate the potential impact bisphenols A, B, S and F on the cell viability and testosterone release in TM3 Leydig cell line. Mice Leydig cells were cultured in the presence of different concentrations of bisphenols (0.04-50 µg.ml-1) during 24 h exposure. Quantification of the cell viability was assessed using the metabolic activity assay, while the level of testosterone in cell culture media was determined by enzyme-linked immunosorbent assay. Within the panel of substances under investigations, the higher experimen...
Source: Physiological Research - July 25, 2019 Category: Physiology Authors: Jambor T, Kovacikova E, Greifova H, Kovacik A, Libova L, Lukac N Tags: Physiol Res Source Type: research

Tempol alleviates chronic intermittent hypoxia-induced pancreatic injury through repressing inflammation and apoptosis.
In this study, we aimed to evaluate the protective role of tempol, a powerful antioxidant, in chronic intermittent hypoxia (IH)-induced pancreatic injury. The rat model of OSA was established by IH exposure. The pathological changes, increased blood-glucose level, and raised proinsulin/insulin ratio in pancreatic tissues of rats received IH were effectively relieved by tempol delivery. In addition, the enhanced levels of pro-inflammatory cytokines, TNF-alpha, IL-1beta, IL-6, and inflammatory mediators, PGE2, cyclooxygenase-2 (COX-2), NO, and inducible nitric oxide synthase (iNOS) in pancreatic tissue were suppressed by tem...
Source: Physiological Research - June 30, 2019 Category: Physiology Authors: Wang Y, Ai L, Hai B, Cao Y, Li R, Li H, Li Y Tags: Physiol Res Source Type: research

Bone microstructure of mice after prolonged taurine treatment.
In this study, a detailed microstructure of compact and trabecular bone tissues of mice subchronically exposed to taurine was determined. Animals (n=12) were segregated into three groups: E1 group - mice received 20 mg/kg b.w. of taurine per day during 8 weeks; E2 group - mice were fed by taurine at a dose of 40 mg/kg b.w. for 8 weeks and a control (C) group. Decreased density of secondary osteons, increased sizes of primary osteon's vascular canals (P
Source: Physiological Research - June 30, 2019 Category: Physiology Authors: Martiniakova M, Sarocka A, Babosova R, Galbavy D, Kapusta E, Goc Z, Formicki G, Omelka R Tags: Physiol Res Source Type: research

Gamma irradiation protect the developing wheat endosperm from oxidative damage by balancing the trade-off between the defence network and grains quality.
Abstract Gamma irradiation has been reported to modulate the biochemical and molecular parameters associated with the tolerance of plant species under biotic/ abiotic stress. Wheat is highly sensitive to heat stress (HS), as evident from the decrease in the quantity and quality of the total grains. Here, we studied the effect of pre-treatment of wheat dry seeds with different doses of gamma irradiation (0.20, 0.25 and 0.30 kGy) on tolerance level and quality of developing wheat endospermic tissue under HS (38 °C, 1 h; continuously for three days). Expression analysis of genes associated with defence and ...
Source: Physiological Research - May 2, 2019 Category: Physiology Authors: Kumar RR, Hasija S, Goswami S, Tasleem M, Sakhare A, Kumar S, Bakshi S, Jambhulkar S, Rai GK, Singh B, Singh GP, Pathak H, Viswanathan C, Praveen S Tags: Ecotoxicol Environ Saf Source Type: research

A method for determination of one hundred endogenous steroids in human serum by gas chromatography-tandem mass spectrometry.
M, Kokrdová Z, Adamcová K, Černý A, Hájek Z, Dušková M, Bulant J, Stárka L Abstract Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized application of the advanced GC-MS/MS platform for the multicomponent quantification of endogenous steroids. We developed and validated a GC-MS/MS method for the quantification of 58 unconjugated...
Source: Physiological Research - April 30, 2019 Category: Physiology Authors: Hill M, Hána V, Velíková M, Pařízek A, Kolátorová L, Vítků J, Škodová T, Šimková M, Šimják P, Kancheva R, Koucký M, Kokrdová Z, Adamcová K, Černý A, Hájek Z, Dušková M, Bulant J, Stárka L Tags: Physiol Res Source Type: research

Transcriptome analysis of juvenile genetically improved farmed tilapia (Oreochromis niloticus) livers by dietary resveratrol supplementation.
Abstract Here we used RNA-Seq to explore the transcriptomic response and specific involvement of hepatic mRNA of juvenile Oreochromis niloticus (GIFT) as a result of dietary resveratrol supplementation (0.05 g/kg RES). More than 24,513,018 clean reads were reference genome guided assembly into 23,417 unigenes. 12,596 unigenes (29.64%) were annotated to GO database. There were 5, 179 and 1526 genes significantly differentially expressed genes at 15, 30 and 45 d respectively, and 8 KEGG pathways were enriched associated with this immune response. Hyperemia and compressed hepatic sinusoid, fibrosis of liver cell ...
Source: Physiological Research - April 24, 2019 Category: Physiology Authors: Zheng Y, Hu G, Wu W, Zhao Z, Meng S, Fan L, Song C, Qiu L, Chen J Tags: Comp Biochem Physiol C Toxicol Pharmacol Source Type: research

Therapeutic Potential of Gamma-Irradiated Resveratrol in Ulcerative Colitis via the Anti-Inflammatory Activity and Differentiation of Tolerogenic Dendritic Cells.
CONCLUSION: Thus, our results suggest that γ-Res may be an excellent candidate for use in IBD treatment. PMID: 30990583 [PubMed - in process] (Source: Physiological Research)
Source: Physiological Research - April 17, 2019 Category: Physiology Authors: Kim WS, Song HY, Mushtaq S, Kim JM, Byun EH, Yuk JM, Byun EB Tags: Cell Physiol Biochem Source Type: research

Identification and characterization of drought responsive miRNAs in a drought tolerant upland rice cultivar KMJ 1-12-3.
Abstract Shortfall of rain that creates drought like situation in non-irrigated agriculture system often limits rice production, necessitating introduction of drought tolerance trait into the cultivar of interest. The mechanism governing drought tolerance is, however, largely unknown, particularly the involvement of miRNAs, the master regulators of biochemical events. In this regard, response study on a drought tolerant rice variety KMJ 1-12-3 to 20% PEG (osmolality- 315 mOsm/kg) as drought stress revealed significant changes in abundance of several conserved miRNAs targeting transcription factors like homeodomain...
Source: Physiological Research - March 10, 2019 Category: Physiology Authors: Awasthi JP, Chandra T, Mishra S, Parmar S, Shaw BP, Nilawe PD, Chauhan NK, Sahoo S, Panda SK Tags: Plant Physiol Biochem Source Type: research

Renal sympathetic denervation in resistant arterial hypertension: long term and updated results.
Šochman J Abstract The present paper is an extension to our earlier publication (Šochman et al. 2016) documenting a beneficial effect of renal sympathetic denervation on pharmacologically uncontrollable hypertension in a group of seven patients followed up for 1-2 years post-procedure. The same patients remained on ambulatory follow-up for another 5-6 years, with the beneficial effect persisting throughout the follow-up period while on the same medication. PMID: 30848155 [PubMed - in process] (Source: Physiological Research)
Source: Physiological Research - March 6, 2019 Category: Physiology Authors: Peregrin JH, Novotný J, Rohál T, Šochman J Tags: Physiol Res Source Type: research

Coenzyme Q10 ameliorates cadmium induced reproductive toxicity in male rats.
This study aimed at investigating the protective role of CoQ10 against cadmium (Cd)-induced reproductive toxicity in male rats. Adult male Wistar rats were exposed to an acute dose of Cd (25 mg/kg bwt; Cd group), Cd+CoQ10 (25 mg/kg bwt Cd+10 mg CoQ10; Cd-Q10 group) and distilled water (control) in vivo for 15 consecutive days and semen quality was assessed. A significant reduction was noted in sperm concentration, progressive motility, morphology and DNA integrity in both Cd- and Cd-Q10 groups in comparison to control indicating Cd-induced testicular lipid per oxidation (LPO) and decline in indigenous antioxidant defense s...
Source: Physiological Research - March 6, 2019 Category: Physiology Authors: Saha R, Roychoudhury S, Kar K, Varghese AC, Nandi P, Sharma GD, Formicki G, Slama P, Kolesarova A Tags: Physiol Res Source Type: research

Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in β-Cell Maintenance.
CONCLUSION: Our study enables us to conclude that β-cell stress is aggravated by the incapability of STAT5B to induce Nos2 resulting in H₂O₂ accumulation and the ensuing oxidative stress enhances β-cell damage. PMID: 30790510 [PubMed - in process] (Source: Physiological Research)
Source: Physiological Research - February 24, 2019 Category: Physiology Authors: Joseph A, Nair LCR, Johnson BS, Thomas PL, Padmanabhan RA, Puthumadathil N, Laloraya M Tags: Cell Physiol Biochem Source Type: research

Resveratrol protects against hepatic insulin resistance in a rat's model of non-alcoholic fatty liver disease by down-regulation of GPAT-1 and DGAT2 expression and inhibition of PKC membranous translocation.
This article is protected by copyright. All rights reserved. PMID: 30773673 [PubMed - as supplied by publisher] (Source: Physiological Research)
Source: Physiological Research - February 17, 2019 Category: Physiology Authors: Badi RM, Mostafa DG, Khaleel EF, Satti HH Tags: Clin Exp Pharmacol Physiol Source Type: research

Effects of dietary resveratrol supplementation during gestation and lactation of sows on milk composition of sows and fat metabolism of sucking piglets.
Abstract The purpose of this article was to investigate the effects of dietary resveratrol supplementation during gestation and lactation of sows on the milk composition of sows and the fat metabolism of sucking piglets. Forty sows were allotted to two experimental treatment groups that included the following: (a) control sows (CON treatment, n = 20) fed with a corn-soybean meal control diet and (b) treatment sows (RES treatment, n = 20) fed with a control diet with addition of 300 mg/kg resveratrol. The results showed that the content of lactose in the colostrum was increased (p 
Source: Physiological Research - February 6, 2019 Category: Physiology Authors: Sun S, Meng Q, Luo Z, Shi B, Bi C, Shan A Tags: J Anim Physiol Anim Nutr (Berl) Source Type: research

Pearl millet stress-responsive NAC transcription factor PgNAC21 enhances salinity stress tolerance in Arabidopsis.
Abstract Pearl millet (Pennisetum glaucum) is the sixth-leading cereal crop and a staple food crop. It is known for its high tolerance to abiotic stress and good nutrient profile. NAC (NAM, ATAF1/2 and CUC) transcription factors (TFs) play an important role in abiotic stress tolerance. In our study, the pearl millet stress-responsive NAC TF gene PgNAC21 was characterized. Gene expression analysis revealed that PgNAC21 expression is induced by salinity stress and abscisic acid (ABA) treatment. In silico promoter analysis showed the presence of ABA response elements (ABREs) and MYB TF binding sites. A yeast one-hybr...
Source: Physiological Research - January 23, 2019 Category: Physiology Authors: Shinde H, Dudhate A, Tsugama D, Gupta SK, Liu S, Takano T Tags: Plant Physiol Biochem Source Type: research

Use of a right ventricular continuous flow pump to validate the distensible model of the pulmonary vasculature.
ten G Abstract In the pulmonary circulation, resistive and compliant properties overlap in the same vessels. Resistance varies nonlinearly with pressure and flow; this relationship is driven by the elastic properties of the vessels. Linehan et al. correlated the mean pulmonary arterial pressure and mean flow with resistance using an original equation incorporating the distensibility of the pulmonary arteries. The goal of this study was to validate this equation in an in vivo porcine model. In vivo measurements were acquired in 6 pigs. The distensibility coefficient (DC) was measured by placing piezo-electric cryst...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Vanden Eynden F, Segers P, Bové T, De Somer F, El Oumeiri B, Van Nooten G Tags: Physiol Res Source Type: research

Vitamin D in autistic children and healthy controls.
acute;ková D, Hanzlíková L Abstract Insufficient levels of vitamin D have been demonstrated by many authors as a risk factor for autistic patients, however, the causality has not been reliably elucidated. In the present study, levels of calcidiol were determined in group of autistic children and compared with age matched healthy children as controls. Suboptimal levels of calcidiol in more than 60 % of both autistic patients as well as of control group were found. No significant differences in vitamin D between autistic children and healthy controls were observed. PMID: 30628824 [PubMed - ...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Bičíková M, Máčová L, Ostatníková D, Hanzlíková L Tags: Physiol Res Source Type: research

Protease-activated receptor-2 regulates glial scar formation via JNK signaling.
Abstract The study aimed to determine the effects of protease-activated receptor-2 (PAR-2) on glial scar formation after spinal cord injury (SCI) in Sprague-Dawley (SD) rats and the underlying mechanisms. Rivlin and Tator's acute extradural clip compression injury (CCI) model of severe SCI was established in this study. Animals were divided into four groups: 1) sham group (laminectomy only); 2) model group, treated with normal saline; 3) PAR-2 inhibitor group; 4) PAR-2 activator group. Enhanced GFAP and vimentin expression were the markers of glial scar formation. To determine whether JNK was involved in the effec...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Li TZ, Deng H, Liu Q, Xia YZ, Darwazeh R, Yan Y Tags: Physiol Res Source Type: research

IGF-1 deletion affects renal sympathetic nerve activity, left ventricular dysfunction, and renal function in DOCA-salt hypertensive mice.
Abstract To determine the influence of IGF-1 deletion on renal sympathetic nerve activity (RSNA), left ventricular dysfunction, and renal function in deoxycorticosterone acetate (DOCA)-salt hypertensive mice. The DOCA-salt hypertensive mice models were constructed and the experiment was classified into WT (Wild-type mice) +sham, LID (Liver-specific IGF-1 deficient mice) + sham, WT + DOCA, and LID+ DOCA groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum IGF-1 levels in mice. The plasma norepinephrine (NE), urine protein, urea nitrogen and creatinine, as well as RSNA were measured. Echoca...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Xiao B, Liu F, Lu JC, Chen F, Pei WN, Yang XC Tags: Physiol Res Source Type: research

The H19/let-7 feedback loop contributes to developmental dysplasia and dislocation of the hip.
In this study, we successfully established a rat model of DDH and demonstrated that H19 was down-regulated in the development of DDH. Further, we constructed H19 knockdown (KD) and overexpression chondrocytes. H19 KD suppressed the proliferation of normal chondrocytes, while overexpression of H19 promoted cell proliferation of DDH chondrocytes. Finally, we revealed that H19 bound to let-7 and inhibited its function, acting as a competing endogenous RNA. Down-regulation of H19 is closely associated with DDH progression and H19 is an important epigenetic factor that regulates the proliferation of chondrocytes. H19 may thus b...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Ning B, Jin R, Wang D, Sun J Tags: Physiol Res Source Type: research

Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia.
Abstract We studied the role of the delta, micro, and kappa opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acute anoxia-reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12% of O(2) for 21 days. Anoxia-reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Naryzhnaya NV, Khaliulin I, Lishmanov YB, Suleiman MS, Tsibulnikov SY, Kolar F, Maslov LN Tags: Physiol Res Source Type: research

Effect of 6-OHDA on hypercapnic ventilatory response in the rat model of Parkinson's disease.
Abstract Breathing impairments, such as an alteration in breathing pattern, dyspnoea, and sleep apnoea, are common health deficits recognised in Parkinson's disease (PD). The mechanism that underlies these disturbances, however, remains unclear. We investigated the effect of the unilateral damage to the rat nigrostriatal pathway on the central ventilatory response to hypercapnia, evoked by administering 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle (MFB). The respiratory experiments were carried out in conscious animals in the plethysmography chamber. The ventilatory parameters were studied in ...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Andrzejewski K, Budzińska K, Kaczyńska K Tags: Physiol Res Source Type: research

Allosteric activation of yeast enzyme neutral trehalase by calcium and 14-3-3 protein.
Abstract Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae catalyzes disaccharide trehalose hydrolysis and helps yeast to survive adverse conditions, such as heat shock, starvation or oxidative stress. 14-3-3 proteins, master regulators of hundreds of partner proteins, participate in many key cellular processes. Nth1 is activated by phosphorylation followed by 14-3-3 protein (Bmh) binding. The activation mechanism is also potentiated by Ca(2+) binding within the EF-hand-like motif. This review summarizes the current knowledge about trehalases and the molecular and structural basis of Nth1 activation. The cr...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Alblova M, Smidova A, Kalabova D, Lentini Santo D, Obsil T, Obsilova V Tags: Physiol Res Source Type: research

Downregulation of endothelial transient receptor potential vanilloid type 4 channel underlines impaired endothelial nitric oxide-mediated relaxation in the mesenteric arteries of hypertensive rats.
Abstract The endothelium contributes to the maintenance of vasodilator tone by releasing endothelium-derived relaxing factors, including nitric oxide (NO). In hypertension, endothelial nitric oxide synthase (eNOS) produces less NO and could be one of the contributing factors to the increased peripheral vascular resistance. Agonist-induced Ca(2+) entry is essential for the activation of eNOS. The transient receptor potential vanilloid type 4 (TRPV4) channel, a Ca(2+)-permeant cation channel, is expressed in the endothelial cells and involved in the regulation of vascular tone. The present study aimed to investigate...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Boudaka A, Al-Suleimani M, Al-Lawati I, Baomar H, Al-Siyabi S, Zadjali F Tags: Physiol Res Source Type: research

The bioimpedance spectroscopy as useful tool for measuring the fluid excess and fluid management in severe polytrauma patients.
Abstract Although the fluid therapy plays a fundamental role in the management of polytrauma patients (PP), a tool which could determine it appropriately is still lacking. The aim of this study was to evaluate the application of a bioimpedance spectroscopy (BIS) for body fluids volume and distribution monitoring in these patients. This prospective, observational study was performed on 25 severe PP and 25 healthy subjects. The body fluids composition was repeatedly assessed using BIS between days 3 to 11 of intensive care unit stay while the impact of fluid intake and balance was evaluated. Fluid intake correlated ...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Joskova V, Patkova A, Havel E, Najpaverova S, Uramova D, Kovarik M, Zadak Z, Hronek M Tags: Physiol Res Source Type: research

Optimal oxygen saturation in extremely premature neonates.
Abstract So far, great efforts have been made to understand the demands of extremely premature neonates (EPNs´; born before the 28(th) week of gestation) on postnatal care, including optimal oxygen saturation, that will allow them to survive without disability. A major yet unresolved problem is to find an "optimal range" of their oxygen saturation and to maintain it without drops or increases, i.e., without hypoxia or hyperoxia. The individual sections of this paper deal with the changes of SpO(2) (an estimate of SaO(2) measured by pulse oximetry) that occur before, during, and after premature labo...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Zoban P Tags: Physiol Res Source Type: research

MENS-associated increase of muscular protein content via modulation of caveolin-3 and TRIM72.
Abstract Microcurrent electrical neuromuscular stimulation (MENS) is known as an extracellular stimulus for the regeneration of injured skeletal muscle in sports medicine. However, the effects of MENS-associated increase in muscle protein content are not fully clarified. The purpose of this study was to investigate the effects of MENS on the muscular protein content, intracellular signals, and the expression level of caveolin-3 (Cav-3), tripartite motif-containing 72 (TRIM72) and MM isoenzyme of creatine kinase (CK-MM) in skeletal muscle using cell culture system. C2C12 myotubes on the 7th day of differentiation p...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Ohno Y, Egawa T, Yokoyama S, Fujiya H, Sugiura T, Ohira Y, Yoshioka T, Goto K Tags: Physiol Res Source Type: research

Gastrointestinal non-motor dysfunction in Parkinson's disease model rats with 6-hydroxydopamine.
Abstract Parkinson's disease (PD) is a neurodegenerative disease with a progressive loss of mesencephalic dopaminergic neurons of the substantia nigra (SN). To further evaluate its pathophysiology, accurate animal models are needed. The current study aims to verify the impact of a 6-hydroxydopamine (6-OHDA) bilateral microinjection into the SN on gastrointestinal symptoms in rats and confirm that the 6-OHDA rat model is an appropriate tool to investigate the mechanisms of Parkinsonian GI disorders. Immunohistochemistry, digital X-ray imaging, short-circuit current, FITC-dextran permeability and ultra-performance l...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Yan JT, Feng XY Tags: Physiol Res Source Type: research

Loss of body weight is accompanying cellular brain edema induced by water intoxication in the rat.
Pokorný J Abstract Induction of cellular cerebral edema (CE) was achieved by a standard method of water intoxication which consisted of fractionated intraperitoneal administration of distilled water (DW) together with the injection of desmopressin (DP). Using metabolic cage, fluid and food balance was studied in two groups of eight animals: group C - control; group CE - cellular edema induced by water intoxication. For each rat the intake (food pellets and water) and excretion (solid excrements and urine) were recorded for 48 h together with the initial and final body weight. CE animals consumed significan...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Kozler P, Marešová D, Pokorný J Tags: Physiol Res Source Type: research

The extracellular matrix and Ca(2+) signaling mechanisms.
thi G Abstract The extracellular matrix (ECM) consists of proteins, glycosaminoglycans and glycoproteins, that support the dynamic interactions between cells, including intercellular communication, cell attachment, cell differentiation, cell growth and migration. As such, the ECM represents an essential and very sensitive system within the tissue microenvironment that is involved in processes such as tissue regeneration and carcinogenesis. The aim of the present review is to evaluate its diversity through Ca(2+) signaling and its role in muscle cell function. Here, we discuss some methodological approaches dissect...
Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Filip S, Mokrý J, Forostyak O, Dayanithi G Tags: Physiol Res Source Type: research