Characterization of a recombinant Aspergillus niger GZUF36 lipase immobilized by ionic liquid modification strategy
In this study, a recombinant extracellular lipase from Aspergillus niger GZUF36 (PEXANL1) expressed in Pichia pastoris GS115 was immobilized on ionic liquid-modified magnetic nano ferric oxide (Fe3O4@SiO2@ILs) via electrostatic and hydrophobic interaction. The morphology, structure, and properties of Fe3O4@SiO2@ILs and immobilized PEXANL1 were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction, vibration sample magnetometer, and zeta potential analysis. Under optimized conditions, the immobilization efficiency and activity recovery of immobilized PEXANL1 were 52 ± 2% ...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Shuqi Xing Jia Long Wei Xie Chaocheng Luo Laping He Cuiqin Li Xuefeng Zeng Source Type: research
Characterization of a recombinant Aspergillus niger GZUF36 lipase immobilized by ionic liquid modification strategy
In this study, a recombinant extracellular lipase from Aspergillus niger GZUF36 (PEXANL1) expressed in Pichia pastoris GS115 was immobilized on ionic liquid-modified magnetic nano ferric oxide (Fe3O4@SiO2@ILs) via electrostatic and hydrophobic interaction. The morphology, structure, and properties of Fe3O4@SiO2@ILs and immobilized PEXANL1 were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction, vibration sample magnetometer, and zeta potential analysis. Under optimized conditions, the immobilization efficiency and activity recovery of immobilized PEXANL1 were 52 ± 2% ...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Shuqi Xing Jia Long Wei Xie Chaocheng Luo Laping He Cuiqin Li Xuefeng Zeng Source Type: research
Identification of closely related species in Aspergillus through Analysis of Whole-Genome
The challenge of discriminating closely related species persists, notably within clinical diagnostic laboratories for invasive aspergillosis (IA)-related species and food contamination microorganisms with toxin-producing potential. We employed Analysis of the whole-GEnome (AGE) to address the challenges of closely related species within the genus Aspergillus and developed a rapid detection method. First, reliable whole genome data for 77 Aspergillus species were downloaded from the database, and through bioinformatic analysis, specific targets for each species were identified. Subsequently, sequencing was employed to valid...
Source: Frontiers in Microbiology - February 21, 2024 Category: Microbiology Source Type: research
Post-COVID pulmonary fungal infections: An unanticipated predicament or a ticking time bomb? Clinico-microbiological profile of cases encountered during the second wave of COVID-19 pandemic at a teaching hospital in the Himalayas with a brief literature review
CONCLUSION: The immuno-compromised state following COVID-19 infection and treatment might be responsible for the progression of regular exposure to the dense Himalayan vegetation into an invasive pulmonary fungal infection. Suspecting post-COVID pulmonary fungal infection is necessary for primary care physicians to ensure timely referral to higher centers. Mixed pulmonary fungal infections (coinfection with Aspergillus spp. and Rhizopus spp.) are also emerging as important sequelae of COVID-19.PMID:38361892 | PMC:PMC10866219 | DOI:10.4103/jfmpc.jfmpc_1073_23 (Source: Primary Care)
Source: Primary Care - February 16, 2024 Category: Primary Care Authors: Oshin Puri Mohit Bhatia Udayakumar S Rekha Deepika Chakraborty Ruchi Dua Minakshi Dhar Udit Chauhan Amber Prasad Deepjyoti Kalita Neelam Kaistha Source Type: research
Post-COVID pulmonary fungal infections: An unanticipated predicament or a ticking time bomb? Clinico-microbiological profile of cases encountered during the second wave of COVID-19 pandemic at a teaching hospital in the Himalayas with a brief literature review
CONCLUSION: The immuno-compromised state following COVID-19 infection and treatment might be responsible for the progression of regular exposure to the dense Himalayan vegetation into an invasive pulmonary fungal infection. Suspecting post-COVID pulmonary fungal infection is necessary for primary care physicians to ensure timely referral to higher centers. Mixed pulmonary fungal infections (coinfection with Aspergillus spp. and Rhizopus spp.) are also emerging as important sequelae of COVID-19.PMID:38361892 | PMC:PMC10866219 | DOI:10.4103/jfmpc.jfmpc_1073_23 (Source: Primary Care)
Source: Primary Care - February 16, 2024 Category: Primary Care Authors: Oshin Puri Mohit Bhatia Udayakumar S Rekha Deepika Chakraborty Ruchi Dua Minakshi Dhar Udit Chauhan Amber Prasad Deepjyoti Kalita Neelam Kaistha Source Type: research
Multifunctional Ag < sub > 2 < /sub > O/chitosan nanocomposites synthesized via sol-gel with enhanced antimicrobial, and antioxidant properties: A novel food packaging material
In this study, a single step in situ sol-gel method was used to syntheses nanocomposite films using chitosan (CS) as the basis material, with the addition of silver oxide nanoparticles (Ag2O) at several weight percentages (5 %, 10 %, and 15 % Ag2O/CS). The structural characteristics of Ag2O/CS films were investigated using a range of analytical techniques. The presence of the primary distinctive peaks of chitosan was verified using FTIR spectra analysis. However, a minor displacement was observed in these peaks due to the chemical interaction occurring with silver oxide molecules. XRD analysis demonstrated a significant in...
Source: International Journal of Biological Macromolecules - February 15, 2024 Category: Biochemistry Authors: Hayat Zagloul Manel Dhahri Ali H Bashal Mona M Khaleil Talaat H Habeeb Khaled D Khalil Source Type: research
Multifunctional Ag < sub > 2 < /sub > O/chitosan nanocomposites synthesized via sol-gel with enhanced antimicrobial, and antioxidant properties: A novel food packaging material
In this study, a single step in situ sol-gel method was used to syntheses nanocomposite films using chitosan (CS) as the basis material, with the addition of silver oxide nanoparticles (Ag2O) at several weight percentages (5 %, 10 %, and 15 % Ag2O/CS). The structural characteristics of Ag2O/CS films were investigated using a range of analytical techniques. The presence of the primary distinctive peaks of chitosan was verified using FTIR spectra analysis. However, a minor displacement was observed in these peaks due to the chemical interaction occurring with silver oxide molecules. XRD analysis demonstrated a significant in...
Source: International Journal of Biological Macromolecules - February 15, 2024 Category: Biochemistry Authors: Hayat Zagloul Manel Dhahri Ali H Bashal Mona M Khaleil Talaat H Habeeb Khaled D Khalil Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: Am J Trop Med Hyg - February 13, 2024 Category: Infectious Diseases Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: The American Journal of Tropical Medicine and Hygiene - February 13, 2024 Category: Tropical Medicine Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: Am J Trop Med Hyg - February 13, 2024 Category: Infectious Diseases Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: The American Journal of Tropical Medicine and Hygiene - February 13, 2024 Category: Tropical Medicine Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: Am J Trop Med Hyg - February 13, 2024 Category: Infectious Diseases Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
The Drivers of Low Vaccination Utilization in Niger
Am J Trop Med Hyg. 2024 Feb 13:tpmd230708. doi: 10.4269/ajtmh.23-0708. Online ahead of print.ABSTRACTVaccination adherence involves the expected benefit of a vaccine and the perceived risk of the disease. To develop an evidence-based communication strategy aimed at improving vaccination coverage in Niger, we conducted a mixed socio-anthropological study of the perceptions among the population on the benefit and the risk of the childhood (Expanded Program on Immunization) and the COVID-19 vaccines. Our results show that negative rumors are not a significant driver of vaccine refusal. The insufficient level of fully vaccinat...
Source: The American Journal of Tropical Medicine and Hygiene - February 13, 2024 Category: Tropical Medicine Authors: Bernard Seytre Sanoussi Chaibou Bernard Simon Source Type: research
