Study on drying methods and their influences on effective components of loquat flower tea
Publication date: September 2015 Source:LWT - Food Science and Technology, Volume 63, Issue 1 Author(s): Meiyu Zheng , Qile Xia , Shengmin Lu Different drying methods and their influences on effective components contents of loquat flower tea were investigated in this study. The determination of total polyphenols (TP), total flavonoids (TF), free amino acid (AA), caffeine and triterpene acids showed relatively higher contents were found at full-bloom stage and in petal tissue. This stage flower was dried to produce loquat flower tea by methods of freeze drying, microwave drying, vacuum drying and hot-air drying, resp...
Source: LWT Food Science and Technology - May 1, 2015 Category: Food Science Source Type: research
Evaluation of the antimutagenic, antigenotoxic, and antioxidant activities of Eriobotrya japonica leaves
Pharmaceutical Biology, Ahead of Print. (Source: Pharmaceutical Biology)
Source: Pharmaceutical Biology - April 16, 2015 Category: Drugs & Pharmacology Tags: article Source Type: research
Isolation and characterization of a catalase gene "HuCAT3" from pitaya (Hylocereus undatus) and its expression under abiotic stress.
Abstract
Abiotic stresses usually cause H2O2 accumulation, with harmful effects, in plants. Catalase may play a key protective role in plant cells by detoxifying this excess H2O2. Pitaya (Hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. However, involvement of the pitaya catalase gene (HuCAT) in tolerance to abiotic stresses is unknown. In the present study, a full-length HuCAT3 cDNA (1870bp) was isolated from pitaya based on our previous microarray data and RACE method. The cDNA sequence and deduced amino acid sequence s...
Source: Gene - March 6, 2015 Category: Genetics & Stem Cells Authors: Nie Q, Gao GL, Fan QJ, Qiao G, Wen XP, Liu T, Peng ZJ, Cai YQ Tags: Gene Source Type: research
Molecules, Vol. 20, Pages 542-555: Phenolic Composition from Different Loquat (Eriobotrya japonica Lindl.) Cultivars Grown in China and Their Antioxidant Properties
In this study, seven loquat cultivars grown in China were evaluated for their phenolic compounds and antioxidant activity. Eleven phenolic compounds, i.e., 3-p-coumaroylquinincacid (3-p-CoQA), 5-caffeoylquinic acid (5-CQA), 4-caffeoylquinic acid (4-CQA), 3-caffeoylquinic acid (3-CQA), 5-feruloylquinic acid (5-FQA), quercetin-3-O-galactoside (Q-3-Gal), quercetin-3-O-glucoside (Q-3-Glu), quercetin-3-O-rhamnoside (Q-3-Rha), kaempferol-3-O-galactoside (K-3-Gal), kaempferol-3-O-rhamnoside (K-3-Rha), and kaempferol-3-O-glucoside (K-3-Glu) were identified and quantified in the peel and pulp of the cultivars tested. 3-CQA and 5-CQ...
Source: Molecules - January 5, 2015 Category: Chemistry Authors: Wenna ZhangXiaoyong ZhaoChongde SunXian LiKunsong Chen Tags: Article Source Type: research
Proteomics approach reveals mechanism underlying susceptibility of loquat fruit to sunburn during color changing period
The objective of this work was to investigate why loquat fruit peels are more sensitive to high temperature and strong sunlight, making them highly susceptible to sunburn, during the color changing period (CCP). Two dimensional gel electrophoresis (2-DE) of the fruit peel proteins was performed over three developmental periods, namely green fruit period (GFP), color changing period and yellow ripening period (YRP). Fifty-five protein spots with at least 2-fold differences in abundance were successfully identified by MALDI-TOF–TOF/MS. The identified proteins were divided into categories related to heat-shock response, str...
Source: Food Chemistry - January 3, 2015 Category: Food Science Source Type: research
Biosorption of Ni(II) from electroplating wastewater by modified (Eriobotrya japonica) loquat bark
Publication date: November 2014 Source:Journal of Saudi Chemical Society, Volume 18, Issue 5 Author(s): Nidá M. Salem , Akl M. Awwad Biosorption of nickel ions from aqueous solutions by modified loquat bark waste (MLB) has been investigated in a batch biosorption process. The biosorbent MLB was characterized by FTIR analysis. The extent of biosorption of Ni(II) ions was found to be dependent on solution pH, initial nickel ions concentration, biosorbent dose, contact time, and temperature. The experimental equilibrium biosorption data were analyzed by three widely used two-parameters Langmuir, Temkin and Freundlich i...
Source: Journal of Saudi Chemical Society - November 27, 2014 Category: Chemistry Source Type: research
Preparation of total flavonoids from loquat flower and its protective effect on acute alcohol-induced liver injury in mice
Conclusion The present findings suggest that TFLF has protective effect on acute alcohol-induced liver injury in mice and may be related to its antioxidant and free-radical scavenging activity. (Source: Journal of Food and Drug Analysis)
Source: Journal of Food and Drug Analysis - November 8, 2014 Category: Food Science Source Type: research
Anti-inflammatory effects and molecular mechanisms of loquat (Eriobotrya japonica) tea
This study aims to clarify the anti-inflammatory effects and molecular mechanisms of loquat tea extract (LTE), in both cell and animal models. LTE, especially C fraction, inhibited the production of pro-inflammatory factors including inducible nitric oxide synthase (iNOS), nitric oxide (NO), IL-6, RANTES and TNF-α. Cellular signaling data revealed that the downregulation of TGF-β-activated kinase (TAK1)-mediated both mitogen-activated protein kinase (MAPK) and NF-κB pathways were involved in the inhibition of pro-inflammatory factors by C fraction. Mouse paws edema model further confirmed the in vivo anti-inflammatory e...
Source: Journal of Functional Foods - November 3, 2014 Category: Nutrition Source Type: research
Molecular evidence for natural hybridization between wild loquat ( Eriobotrya japonica ) and its relative E. prinoides
Conclusions:
Our study provides compelling evidence for a hybrid status for E. prinoides var. daduheensis. Most hybrid individuals are later-generation hybrids. Both E. japonica and E. prinoides can serve as female parent. Differential adaptation might maintain the species boundary of E. prinoides and E. japonica in the face of hybridization and potential introgression. (Source: BMC Plant Biology)
Source: BMC Plant Biology - October 10, 2014 Category: Biology Authors: Qiang FanSufang ChenMingwan LiWei GuoHuijuan JingWei WuRenchao ZhouWenbo Liao Source Type: research
In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells.
This study aimed to clarify the chemopreventive effect of loquat tea extract (LTE) by investigating the inhibition on proliferation, and underlying mechanisms in human promyelocytic leukemia cells (HL-60). LTE inhibited proliferation of HL-60 in a dose-dependent manner. Molecular data showed that the isolated fraction of LTE induced apoptosis of HL-60 as characterized by DNA fragmentation; activation of caspase-3, -8, and -9; and inactivation of poly(ADP)ribose polymerase. Moreover, LTE fraction increased the ratio of pro-apoptotic Bcl-2-associated X protein (Bax)/anti-apoptotic myeloid cell leukemia 1 (Mcl-1) that caused ...
Source: Bioscience, Biotechnology, and Biochemistry - August 13, 2014 Category: Biochemistry Authors: Zar PP, Yano S, Sakao K, Hashimoto F, Nakano T, Fujii M, Hou DX Tags: Biosci Biotechnol Biochem Source Type: research
Anti-epidermal growth factor receptor tyrosine kinase activities of traditional Chinese medicine for cancer treatment
Conclusion: Our results indicate that screening traditional Chinese herbs for EGFR inhibitory activity may identify a large number of potential lead compounds for the development of new drugs to treat cancer. (Source: European Journal of Integrative Medicine)
Source: European Journal of Integrative Medicine - June 26, 2014 Category: Complementary Medicine Authors: Zenghui Wang, Sensen Lin, Dongmei Wang, Linfang Huang Tags: Original articles Source Type: research
Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy.
Abstract
Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products. After analysis of 24 red-fleshed loquat accessions, we found that only 7 pairs of primers could clearly separate all of them. A cult...
Source: Genetics and Molecular Research - May 26, 2014 Category: Genetics & Stem Cells Authors: Li XY, Xu HX, Chen JW Tags: Genet Mol Res Source Type: research
RNA isolation from loquat and other recalcitrant woody plants with high quality and yield.
Abstract
RNA isolation is difficult in plants that contain large amounts of polysaccharides and polyphenol compounds. To date, no commercial kit has been developed for the isolation of high-quality RNA from tissues with these characteristics, especially for fruit. The common protocols for RNA isolation are tedious and usually result in poor yields when applied to recalcitrant plant tissues. Here an efficient RNA isolation protocol based on cetyltrimethylammonium bromide (CTAB) and two successive precipitations with 10 M lithium chloride (LiCl) was developed specifically for loquat fruits but it was proved ...
Source: Analytical Biochemistry - February 17, 2014 Category: Biochemistry Authors: Morante-Carriel J, Sellés-Marchart S, Martínez-Márquez A, Martínez-Esteso MJ, Luque I, Bru-Martínez R Tags: Anal Biochem Source Type: research
Meat quality traits of four Chinese indigenous chicken breeds and one commercial broiler stock.
In conclusion, there were significant differences in the meat quality traits of the bird breeds selected in this study, and the indigenous chickens, especially the NC genotype, produced better quality meat as far as the IMP content, fiber diameters, and shear forces were concerned.
PMID: 24101206 [PubMed - as supplied by publisher] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - October 1, 2013 Category: Science Authors: Guan RF, Lyu F, Chen XQ, Ma JQ, Jiang H, Xiao CG Tags: J Zhejiang Univ Sci B Source Type: research
The flavonoid myricetin reduces nocturnal melatonin levels in the blood through the inhibition of serotonin N-acetyltransferase.
Abstract
Melatonin is secreted during the hours of darkness and is thought to influence the circadian and seasonal timing of a variety of physiological processes. AANAT, which is expressed in the pineal gland, retina, and various other tissues, catalyzes the conversion of serotonin to N-acetylserotonin and is the rate-limiting enzyme in the biosynthetic pathway of melatonin. The compounds that modulate the activity of AANAT can be used to treat patients with circadian rhythm disorders that are associated with specific circadian rhythm alterations, such as shift work disorder. In the present study, we scree...
Source: Biochemical and Biophysical Research communications - September 25, 2013 Category: Biochemistry Authors: Shin JC, Jung HY, Harikishore A, Kwon OD, Yoon HS, Kim KT, Choi BH Tags: Biochem Biophys Res Commun Source Type: research
