Acquired Glanzmann thrombasthenia: a rare disorder
We describe a patient of ITP with borderline low platelet count and severe bleeding, who posed a diagnostic challenge. However, an accurate diagnosis and suitable management helped to avoid catastrophic bleeding. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - October 16, 2020 Category: Pathology Source Type: research
Expression of CD180 in common B cell neoplasms using immunohistochemistry
AbstractDiagnosis of low-grade B cell lymphoma can be challenging especially on limited cytological and histological samples, CD180 is Toll-like receptor that was found to be of value in the diagnosis of marginal zone lymphoma using flow cytometry. In this pilot study, we examine the expression of CD180, using an immunohistochemical method, on a number of common B cell lymphoproliferative neoplasms. Immunohistochemical staining for sixty-seven cases of five B cell neoplasms, specifically, marginal zone lymphoma, mantle cell lymphoma, diffuse large B cell lymphoma, follicular lymphoma, and chronic lymphocytic leukemia/small...
Source: Journal of Hematopathology - October 13, 2020 Category: Pathology Source Type: research
Clinical laboratory validation of the MCL35 assay for molecular risk stratification of mantle cell lymphoma
In this study, we sought to explore the performance characteristics of the MCL35 assay in a clinical laboratory and compare r esults with the Ki67 proliferation marker. The results describe the clinical validation of the MCL35 assay for molecular risk stratification of MCL including accuracy, sensitivity, specificity, use in acid-decalcified bone marrow core biopsies, fixatives, lower limit of RNA input, quality metrics, a nd other laboratory parameters. The resulting data indicate that this is a robust technique with outstanding reproducibility. Overall, the data support the concept of molecular signatures, as assessed wi...
Source: Journal of Hematopathology - October 13, 2020 Category: Pathology Source Type: research
Elevated eosinophil count is related with lower anti-factor Xa activity in COVID-19 patients
In conclusion; Elevated eosinophil count is related to lower anti-factor Xa activity in patients with COVID-19 receiving LMWH. The clinical significance of the subprophylactic anti-factor Xa activity should be studied in COVID-19 patients (NCT04507282). (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - October 8, 2020 Category: Pathology Source Type: research
Risk factors for hypogammaglobulinemia in chronic lymphocytic leukemia patients treated with anti-CD20 monoclonal antibody-based therapies
ConclusionHypogammaglobinemia is commonly observed in CLL patients, at baseline as well as with the additional use of anti-CD20 therapies. The mean time to onset of hypogammaglobinemia after anti-CD20 therapies was 36 Â months. Time to hypogammaglobinemia does not significantly differ based on any of the CLL clinical factors studied. The presence of hypogammaglobinemia did not appear to impact survival or time to infection. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - September 30, 2020 Category: Pathology Source Type: research
HBB gene mutation spectrum in an Indian cohort of 1530 cases using an in-house targeted next-generation sequencing assay
AbstractBeta thalassemia major is a common genetic disorder characterized by the reduced production or absence of beta globin, a product of the haemoglobin subunit beta (HBB) gene. Every year, approximately 10,000 –12,000 children with thalassemia major are born in India. Molecular methodologies like ARMS (amplification-refractory mutation system)-PCR (polymerase chain reaction) and capillary sequencing are used to detectHBB gene mutations. It is, however, challenging to achieve comprehensive coverage of theHBB gene by these methods. Next-generation sequencing (NGS) can be used to circumvent these problems. Commercial NG...
Source: Journal of Hematopathology - September 29, 2020 Category: Pathology Source Type: research
Leukocytoclastic vasculitis as a previously unreported paraneoplastic manifestation of acute lymphoblastic leukemia in adults
ConclusionThis case is the first adult ALL case with paraneoplastic LCV. In this study, it is emphasized that hematological malignancies should be considered in patients presenting with cutaneous vasculitis, especially in the presence of cytopenia. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - September 28, 2020 Category: Pathology Source Type: research
TBL1XR1-JAK2: a novel fusion in a pediatric T cell acute lymphoblastic leukemia patient with increased absolute eosinophil count
AbstractT-lymphoblastic leukemia/lymphoma (T-ALL/LBL) with anyJAK2 gene fusion is rarely reported. Here, we report a case of T-ALL with a novelTBL1XR1-JAK2 gene fusion in a 5-year-old boy. His lab showed a high white blood cell count, mild anemia, moderate thrombocytopenia, and concurrently increased eosinophils (absolute eosinophil count: 4  × 10^9/L). Peripheral blood and bone marrow aspirate smears showed>  90% mononucleated blasts. Flow cytometry on peripheral blood revealed a large blast population positive for CD2, surface CD3 (<  25%), CD10 (50%), CD5, CD7, CD4, CD8, TdT, CD1a (60%), and CD45. Convent...
Source: Journal of Hematopathology - August 31, 2020 Category: Pathology Source Type: research
Relationship between p53 expression and prognosis of myelodysplastic syndrome treated with azacitidine
AbstractWe previously demonstrated that a CD13/CD33 ratio low (<  1) and high (≥ 2) was associated with shorter survival in patients with myelodysplastic syndromes (MDS) treated with azacitidine (AZA). Previous studies also showed the negative impact of TP53 mutations on patient outcomes. The aim of the study is to investigate the relationship between a p53 expression, CD13/CD33 ratio, and the outcomes of MDS patients treated with AZA. The relationship between the p53 expression, CD13/CD33 ratio in blast cells, and outcomes of 121 MDS patients treated with AZA was examined. In patients with CD13/CD33 ratio low and...
Source: Journal of Hematopathology - August 19, 2020 Category: Pathology Source Type: research
Assessing for disease: recurrent mycosis fungoides or cutaneous granulomatous drug eruption after mogamulizumab therapy
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - August 11, 2020 Category: Pathology Source Type: research
Histopathological and clinical features of nodular lymphocyte-predominant Hodgkin lymphoma and their impact on prognosis: first report from Iran
This study aimed to investigate the clinicopathological features of NLPHL cases in Shiraz, Iran, and their possible impact on prognosis. Between March 2014 and November 2019, all records for lymphoma were extracted from the electronic files of hospitals affiliated with Shiraz University of Medical Sciences. The slides of all NLPHL cases were extracted and reviewed. Other necessary data was retrieved from hospital records and through direct contact with patients. Among1168 lymphoma cases, 382 were HLs and 19.1% (73) of them were of the NLPHL type. The median age of NLPHL patients was 34 Â years. Of the patients, 51.5% showe...
Source: Journal of Hematopathology - July 30, 2020 Category: Pathology Source Type: research
Acute promyelocytic leukemia in a child with reticulin fibrosis
This report presents a case of APL in a pediatric patient in association with significant reticulin fibrosis and osteosclerosis. The case highlights that fibrosis in the marrow can make it more challenging to diagnose acute leukemia. ThePML-RARA transcripts can be present with subtle clinical or hematological findings, emphasizing the importance of molecular studies in unexplained cytopenias. Furthermore, in the case of APL, as opposed to other myeloid neoplasms, fibrosis does not appear to be associated with a worse prognosis and can resolve after treatment. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 21, 2020 Category: Pathology Source Type: research
Plasmablastic lymphoma presenting as a soft tissue mass associated with a chronic wound in an immunocompetent patient
AbstractPlasmablastic lymphoma (PBL) is a rare aggressive type of non-Hodgkin lymphoma that primarily affects human immunodeficiency virus (HIV) –positive patients but can arise in other immunocompromised states such as transplant recipients, as well as in immunocompetent patients. The neoplastic cells express an immunophenotype that resembles plasma cells, in that they usually do not express CD20 (or may be weakly positive) but do express markers of plasmacytic differentiation such as CD38, CD138, or MUM1/IRF4. These tumors generally have high mitotic activity, evidenced by a high Ki-67 proliferation index. Epstein-Barr...
Source: Journal of Hematopathology - July 10, 2020 Category: Pathology Source Type: research
Prevalence of β-haemoglobinopathies in Eastern India and development of a novel formula for carrier detection
This study provides an audit of the common β-hemoglobinopathies in Eastern India. The regional variations and prevalence of hemoglobinopathies highlighted here may guide public health efforts. The formula generated from RBC indices can be used to screen individuals pr ior to HPLC testing, thereby reducing overall costs of thalassemia screening in the country. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 7, 2020 Category: Pathology Source Type: research
IgH gene rearrangement by PCR as an adjunct to flow cytometric analysis for the detection of minimal residual disease in patients with B lymphoblastic leukemia
AbstractTo examine the benefits of performing flow cytometry and PCR forIGH gene rearrangement in parallel for the detection of minimal residual disease (MRD) in patients with B lymphoblastic leukemia. A flow cytometric assay for MRD and PCR-based assay forIGH gene rearrangement were performed, concurrently, on bone marrow evaluations from patients with a new or previous diagnosis of B-ALL from Oct 1, 2016, to Mar 25, 2019. The results were compared by the kappa (K) statistic. A total of 119 cases had concurrent flow cytometry and PCR for monoclonalIGH gene rearrangement performed. The overall percent agreement was 84% (Kâ...
Source: Journal of Hematopathology - July 2, 2020 Category: Pathology Source Type: research
