TBL1XR1-JAK2: a novel fusion in a pediatric T cell acute lymphoblastic leukemia patient with increased absolute eosinophil count
AbstractT-lymphoblastic leukemia/lymphoma (T-ALL/LBL) with anyJAK2 gene fusion is rarely reported. Here, we report a case of T-ALL with a novelTBL1XR1-JAK2 gene fusion in a 5-year-old boy. His lab showed a high white blood cell count, mild anemia, moderate thrombocytopenia, and concurrently increased eosinophils (absolute eosinophil count: 4  × 10^9/L). Peripheral blood and bone marrow aspirate smears showed>  90% mononucleated blasts. Flow cytometry on peripheral blood revealed a large blast population positive for CD2, surface CD3 (
Source: Journal of Hematopathology - September 1, 2020 Category: Pathology Source Type: research

Relationship between p53 expression and prognosis of myelodysplastic syndrome treated with azacitidine
AbstractWe previously demonstrated that a CD13/CD33 ratio low (
Source: Journal of Hematopathology - August 20, 2020 Category: Pathology Source Type: research

Assessing for disease: recurrent mycosis fungoides or cutaneous granulomatous drug eruption after mogamulizumab therapy
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - August 12, 2020 Category: Pathology Source Type: research

Histopathological and clinical features of nodular lymphocyte-predominant Hodgkin lymphoma and their impact on prognosis: first report from Iran
This study aimed to investigate the clinicopathological features of NLPHL cases in Shiraz, Iran, and their possible impact on prognosis. Between March 2014 and November 2019, all records for lymphoma were extracted from the electronic files of hospitals affiliated with Shiraz University of Medical Sciences. The slides of all NLPHL cases were extracted and reviewed. Other necessary data was retrieved from hospital records and through direct contact with patients. Among1168 lymphoma cases, 382 were HLs and 19.1% (73) of them were of the NLPHL type. The median age of NLPHL patients was 34  years. Of the patients, 51.5% s...
Source: Journal of Hematopathology - July 31, 2020 Category: Pathology Source Type: research

Acute promyelocytic leukemia in a child with reticulin fibrosis
This report presents a case of APL in a pediatric patient in association with significant reticulin fibrosis and osteosclerosis. The case highlights that fibrosis in the marrow can make it more challenging to diagnose acute leukemia. ThePML-RARA transcripts can be present with subtle clinical or hematological findings, emphasizing the importance of molecular studies in unexplained cytopenias. Furthermore, in the case of APL, as opposed to other myeloid neoplasms, fibrosis does not appear to be associated with a worse prognosis and can resolve after treatment. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 22, 2020 Category: Pathology Source Type: research

Plasmablastic lymphoma presenting as a soft tissue mass associated with a chronic wound in an immunocompetent patient
AbstractPlasmablastic lymphoma (PBL) is a rare aggressive type of non-Hodgkin lymphoma that primarily affects human immunodeficiency virus (HIV) –positive patients but can arise in other immunocompromised states such as transplant recipients, as well as in immunocompetent patients. The neoplastic cells express an immunophenotype that resembles plasma cells, in that they usually do not express CD20 (or may be weakly positive) but do express markers of plasmacytic differentiation such as CD38, CD138, or MUM1/IRF4. These tumors generally have high mitotic activity, evidenced by a high Ki-67 proliferation index. Epstein-...
Source: Journal of Hematopathology - July 11, 2020 Category: Pathology Source Type: research

Prevalence of β-haemoglobinopathies in Eastern India and development of a novel formula for carrier detection
This study provides an audit of the common β-hemoglobinopathies in Eastern India. The regional variations and prevalence of hemoglobinopathies highlighted here may guide public health efforts. The formula generated from RBC indices can be used to screen individuals pr ior to HPLC testing, thereby reducing overall costs of thalassemia screening in the country. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 8, 2020 Category: Pathology Source Type: research

IgH gene rearrangement by PCR as an adjunct to flow cytometric analysis for the detection of minimal residual disease in patients with B lymphoblastic leukemia
AbstractTo examine the benefits of performing flow cytometry and PCR forIGH gene rearrangement in parallel for the detection of minimal residual disease (MRD) in patients with B lymphoblastic leukemia. A flow cytometric assay for MRD and PCR-based assay forIGH gene rearrangement were performed, concurrently, on bone marrow evaluations from patients with a new or previous diagnosis of B-ALL from Oct 1, 2016, to Mar 25, 2019. The results were compared by the kappa (K) statistic. A total of 119 cases had concurrent flow cytometry and PCR for monoclonalIGH gene rearrangement performed. The overall percent agreement was 84% (K&...
Source: Journal of Hematopathology - July 3, 2020 Category: Pathology Source Type: research

Activation of cytotoxic T cell population and inversion of CD4:CD8 ratio as manifestations of cellular immune response in SARS-COV-2 infection
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 2, 2020 Category: Pathology Source Type: research

A practical approach to FISH testing for MYC rearrangements and brief review of MYC in aggressive B-cell lymphomas
AbstractAggressive B-cell lymphomas, including the WHO diagnoses of diffuse large B-cell lymphoma, high-grade B-cell lymphoma withMYC andBCL2 and/orBCL6 rearrangements, high-grade B-cell lymphoma, not otherwise specified, and Burkitt lymphoma, together account for approximately 40% of B-cell non-Hodgkin lymphomas. Identification ofMYC,BCL2, andBCL6 rearrangements in these neoplasms is critical for diagnostic, prognostic, and therapeutic purposes. Herein, we address technical issues surrounding identification of these genetic abnormalities, discuss their diagnostic, prognostic, and therapeutic implications, and present an a...
Source: Journal of Hematopathology - June 18, 2020 Category: Pathology Source Type: research

Pediatric post-transplant lymphoproliferative disorder identified on random gastrointestinal biopsies shows non-mass-forming PTLD with clonal plasma cells: report of 2 cases
AbstractEBV-related lymphoproliferations in post-transplant settings range from non-destructive hyperplasia to polymorphic post-transplant lymphoproliferative disorder (PTLD) to overt lymphoma. Here we present two unusual cases of pediatric non-mass-forming gastrointestinal (GI) PTLDs with mixed pathologic features of non-destructive and polymorphic categories. Both patients were EBV na ïve and presented within 1 year of transplant with non-specific gastrointestinal symptoms (diarrhea, abdominal pain, and food intolerance) with hypoalbuminemia, cytopenia, and EBV viremia. No mass lesions were found on imaging and...
Source: Journal of Hematopathology - June 12, 2020 Category: Pathology Source Type: research

Mucosal intralymphatic spread in a relapsed diffuse large B cell lymphoma
AbstractIntralymphatic spread is a rare finding and is associated with poor prognosis in diffuse large B cell lymphoma (DLBCL). Here, we report a case of relapsed DLBCL with mucosal intralymphatic spread. A 69-year-old man had been diagnosed with gastric DLBCL stage IIE at 57  years. He had a relapse with generalized lymphadenopathy and extranodal involvement at 61 years; then, second complete remission was achieved after salvage chemotherapy. He then had a second relapse with involvement of the terminal ileum, spinal cord, and left tonsil. The terminal ileum showed in tralymphatic spread in the lamina propria of...
Source: Journal of Hematopathology - June 12, 2020 Category: Pathology Source Type: research

Transformation of de novo high-grade B cell lymphoma with MYC and BCL2 rearrangements to double-hit B lymphoblastic leukemia/lymphoma: a case report and review of literature
AbstractHigh-grade B cell lymphomas withMYC andBCL2 and/orBCL6 rearrangements ( “double-hit” lymphoma (DHL)/“triple-hit” lymphoma (THL)) are mature B cell neoplasms with highly aggressive clinical behavior and poor response to therapy. Lymphoblastic transformation of mature B cell neoplasms is an uncommon event that is best recognized for follicular lymphoma (FL). To ou r knowledge, only one case of “B lymphoblastic” transformation of de novo DHL has been reported in the literature. Here, we describe another case of such transformation. The patient was a 57-year-old man who presented wit...
Source: Journal of Hematopathology - June 6, 2020 Category: Pathology Source Type: research

Mantle cell lymphoma: pathologic and immunophenotyping study of 50 cases and correlation with survival —a single institute experience
This study focuses on (1) age, gender, and location of tumor at presentation; (2) some challenges in the diagnostic criteria; (3) cytomorphology, histomorphology, immunophenotyping, and proliferation rate; and (4) overall survival and finally relationship with demographic and pathologic findings which shows no association between survival of patients and gender, cytomorphology, histology, and location of tumor while increased mitotic activity and age are prognostically important factors. Cyclin D1 immunostaining is strictly recommended in all low-grade B cell lymphoma due to absence of CD5 expression on 20% of confirmed MC...
Source: Journal of Hematopathology - May 29, 2020 Category: Pathology Source Type: research

The molecular analysis of four coexistent mutations in additional sex combs like 1 (ASXL1) gene in a patient with acute myeloid leukemia
AbstractMutations in the additional sex combs like 1 (ASXL1) gene are frequently involved in clonal hematopoiesis and are connected with an increased risk of hematologic cancer. These aberrations are frequently detected in a number of different hematological neoplasms including acute myeloid leukemia (AML). Patients harboringASXL1 mutations tend to have a poor prognosis and poor response to therapy. Here, we report the coexistence of four different mutations inASXL1 gene in a patient with AML. Such accumulation of mutations, in this gene, has not been described yet. Our findings suggest that accumulations of mutations inAS...
Source: Journal of Hematopathology - May 27, 2020 Category: Pathology Source Type: research

Osteopetrosis in a six-month-old infant
We report a six-month-old male with osteopetrosis where bone marrow examination and molecular studies allowed a definitive diagnosis. Microscopic examination of a bone marrow biopsy demonstrated increased bone formation at various stages of ossification, decreased marrow spaces, and increased osteoclasts rimming the bone. Next-generation sequencing (NGS) demonstrated that the patient was homozygous for mutations in theTCIRG1 gene, which encodes an osteoclast vacuolar proton pump and is the most common mutation associated with the autosomal recessive or infantile malignant form of osteopetrosis. The patient underwent a cord...
Source: Journal of Hematopathology - May 27, 2020 Category: Pathology Source Type: research

COVID-19 and remembering Professor Dennis H. Wright
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - May 26, 2020 Category: Pathology Source Type: research

Pacinian corpuscle in human lymph node: a report and the literature review
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - May 22, 2020 Category: Pathology Source Type: research

Reticulocyte count and its parameters: comparison of automated analyzers, flow cytometry, and manual method
In this study, we evaluated the three methods of reticulocyte counting, namely automated, flow cytometry, and manual methods for reticulocyte count and also for the enumeration of immature reticulocyte fraction (IRF). Reticulocyte count was done by manual method (light microscopy); two different automated analyzers, LH-780, Beckman Coulter, USA and Pentra-XLR, Horiba Ltd., Japan; and flow cytometry (FACS Canto II, B.D. Bioscience, USA). Also, the classification of stages of maturation was done, and percentage was counted based on quantity of reticulum and its distribution in the cytoplasm. Statistical analysis was done to ...
Source: Journal of Hematopathology - May 21, 2020 Category: Pathology Source Type: research

Enumeration of CD34+ blasts by immunohistochemistry in bone marrow biopsies from MDS patients may have significant impact on final WHO classification
AbstractThe percentage of blasts cells in the bone marrow (BM) of MDS patients is one of the key parameters for MDS classification and for the differential diagnosis with acute myeloid leukemia (AML). Currently, the gold standard to determine the blast percentage is conventional cytomorphology. To assess the possible impact of blast cell enumeration in BM biopsies from MDS patients on the final WHO classification using CD34 immunohistochemistry (IHC) a total of 156 BM samples from MDS and MDS-AML patients were studied  and compared to blast counts by cytomorphology (CM). Eighty-nine BM aspirates were also studied by f...
Source: Journal of Hematopathology - April 23, 2020 Category: Pathology Source Type: research

Peripheral T cell lymphoma, NOS with aberrant αβ and γδ T cell receptor expression in a post-heart transplant patient
AbstractThree main patterns of T cell receptor (TCR) expression are identified in peripheral T cell lymphoma (PTCL): TCR- αβ type, TCR-γδ type, and TCR silent type. Cases of PTCL that express both αβ and γδ receptors are rare. Here, we report a case of PTCL-NOS (not otherwise specified) with dual αβ and γδ expression in a 61-year-old female 6 years after orthotopic cardiac transplant. The patient presented with fatigue, fever, worsening anemia, and thrombocytopenia. A chest CT showed multiple irregular lung nodules and diffuse lymphadenopathy. Cytolo...
Source: Journal of Hematopathology - April 2, 2020 Category: Pathology Source Type: research

Immunophenotypic and molecular analysis of a B cell lymphoma with discordant light chain expression at different anatomic sites
We report a case of a 75-year-old woman with a high-grade B cell lymphoma with MYC and BCL2 rearrangements (HGBCL) with discordant immunoglobulin light chain restriction between a lymph node and a pleural fluid involved by lymphoma. Cytogenetic studies by FISH showed MYC and BCL2 rearrangement in both specimens. Immunoglobulin heavy chain and kappa light chain analysis demonstrated similar rearranged clonal peaks in the two specimens. A review of the literature revealed eight cases of B cell lymphoma with discordant immunoglobulin light chain expression (including our case). The light chain discrepancy occurred during a re...
Source: Journal of Hematopathology - March 10, 2020 Category: Pathology Source Type: research

Does the order of mutational acquisition in myeloproliferative neoplasms matter? Evidence from JAK2 exon 12 and DNMT3A co-mutant polycythemia vera
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - March 5, 2020 Category: Pathology Source Type: research

Histoplasma capsulatum in peripheral blood neutrophils
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - March 2, 2020 Category: Pathology Source Type: research

Twenty years later: has cell of origin testing in diffuse large B cell lymphoma run its course?
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - February 28, 2020 Category: Pathology Source Type: research

Molecular spectrum and distribution of hemoglobinopathies in southwest of Iran: a seven-year retrospective study
This study aimed to assess a comprehensive prevalence of most frequent thalassemia mutations in Khuzestan Province, where is a belt of thalassemia in Iran. A total of 6946 subjects were enrolled for evaluating alpha-beta thalassemia from 2012 to 2018. In order to determine the silent mutations, subjects with microcytic hypochromic without anemia with normal Hb, HbA2, and HbF were included too. Genomic DNA was extracted, and ARMS-PCR, Gap-PCR, and DNA sequencing were used to detect thalassemia mutations. Of 6946 individuals, just 880 (12.6%) were normal, and 6066 (87.3%) were the carrier for thalassemia. The most frequent p...
Source: Journal of Hematopathology - February 17, 2020 Category: Pathology Source Type: research

Reviewers for Journal of Hematopathology in 2019
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 27, 2020 Category: Pathology Source Type: research

Mast cell leukemia with t(8;21) and t(12;13): can we classify it as acute mast cell leukemia?
We report the case of a 69-year-old man who presented acute leukemia with circulating blasts and numerous atypical mast cells in peripheral blood. Both blasts and mast cells presented identical cytochemistry (myeloperoxidase-negative, tartrate-resistant acid phosphatase –negative, esterase-negative; toluidine blue–positive; and acid phosphatase–positive). Flow cytometry showed a blast population MPO−, DR−, CD34−, CD117++, CD25+, and CD2−. Bone marrow histology revealed infiltration by CD25+, c-Kit+, and tryptase+ cells. Cytogenetics revealed 46,XY,t(8; 21)(q22; q22),der(12),t(12; 1...
Source: Journal of Hematopathology - January 21, 2020 Category: Pathology Source Type: research

Papular eruption characteristic of persistent agmination of lymphomatoid papulosis
AbstractA 48-year-old man presented with a 1-year history of a nontender, pruritic, well-circumscribed crop of waxing and waning papules. The histological findings were compatible with lymphomatoid papulosis (LyP) in spite of the fact that cutaneous distribution was not typical. Attending to the clinicopathological correlation, we established the diagnosis of persistent agmination of lymphomatoid papulosis (PALP). PALP is a rare variant of CD30+ primary cutaneous T cell lymphoproliferative disorder, with only a few reported cases in the literature. It is still controversial whether PALP is a subtype of localized LyP or an ...
Source: Journal of Hematopathology - January 20, 2020 Category: Pathology Source Type: research

Mantle cell lymphoma mimicking chronic lymphocytic leukemia/small lymphocytic lymphoma on flow cytometry
AbstractOne of the most common applications of clinical flow cytometry is to diagnose chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). When classic features are present and correlation with other diagnostic modalities is possible, the distinction of CLL/SLL from other subtypes of B-cell lymphoma is straightforward. However, features deviating from prototypical phenotypes occur not infrequently and can present a diagnostic dilemma. One potential pitfall that has not been well described is mimicry of CLL/SLL by mantle cell lymphoma. A review of flow cytometric data and corresponding clinical, morphologic, a...
Source: Journal of Hematopathology - January 18, 2020 Category: Pathology Source Type: research

CD56-positive B cell precursor acute lymphoblastic leukemia harboring KMT2A-AFF1 rearrangement developed in a pregnant woman successfully treated with allogeneic hematopoietic cell transplantation
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 7, 2020 Category: Pathology Source Type: research

Decreased activity of plasma ADAMTS-13 predicts poor prognosis in acute lymphoblastic leukemia patients after hematopoietic stem cell transplant
AbstractDecreased ADAMTS-13 is important for the diagnosis of thrombotic thrombocytopenic purpura (TTP), but recent studies have shown that reduced ADAMTS-13 activity is associated with disease prognosis of a variety of cancer patients. As an important hematological malignancy, the significance of ADAMTS-13 in acute lymphoblastic leukemia (ALL) has not been fully elucidated. This research aims to explore the significance of decreased ADAMTS-13 activity in acute lymphoblastic leukemia after hematopoietic stem cell transplant (HSCT). Thirty-eight ALL patients were included in this research, and their ADAMTS-13 activity was m...
Source: Journal of Hematopathology - January 3, 2020 Category: Pathology Source Type: research

Hyperleucocytosis grading score and NPM1 gene mutation among patients with acute myeloid leukemia: Malaysian experience
AbstractThis is the first study evaluating the frequencies of nucleophosmin (NPM1) gene mutation according to the hyperleukocytosis (HL) grading score. The current two cut off values of this score in acute myeloid leukaemia (AML) patients are [1] those with 50 –100 × 109/L and [2] those with ≥ 100 × 109/L total WBC count. The score represents the current clinically based definition of HL in patients with AML. A total of 90 patients with AML were included. AML patients were stratified into three groups: [1] those with a WBC count below 50  × 109/L (...
Source: Journal of Hematopathology - January 3, 2020 Category: Pathology Source Type: research

Multiplexing 8 colors with 12 antibodies in a single lymphoid screening tube by flow cytometry for evaluating suspected chronic lymphoproliferative disorders (CLPD)
AbstractThe diagnosis of chronic lymphoproliferative disorder (CLPD) or non-Hodgkin Lymphoma (NHL) is based on the detection of the abnormal clonal lymphoid cells. The flow cytometry (FCM) immunophenotyping not only plays an essential role in the screening of CLPD but also helps in the specific identification and characterization of the expanded aberrant lymphocytes. Over decades, it has evolved from a single parameter to multi-parameter assessment by 3- to 12-color FCM. The greatest challenge is to characterize abnormal lymphoid cells by a limited immunophenotype (IPT) panel. A study was undertaken to evaluate the diagnos...
Source: Journal of Hematopathology - December 2, 2019 Category: Pathology Source Type: research

A case of an unusual lineage switch in late relapse ALL —is it actually a secondary leukemia?
AbstractAcute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid precursors. According to immunophenotype, it is further subdivided into precursor B cell ALL and precursor T cell ALL, with precursor B cell ALL being much more common both in children and adults. Lineage switch from one lymphoid lineage to another during the course of the disease is extremely rarely reported. Here, we describe a case of a child who initially presented as a precursor B-ALL but 15  years later and after two successfully treated relapses of the original ALL presented with early T cell precursor leukemia. Although it was consid...
Source: Journal of Hematopathology - November 20, 2019 Category: Pathology Source Type: research

Primary pulmonary extranodal NK/T cell lymphoma of an elderly adult: a case report and literature review
AbstractExtranodal natural killer/T-cell lymphoma (ENKTCL), nasal type, is rare and aggressive and often involves the nose, nasopharynx, and upper aerodigestive tract. The non-nasal type can affect the skin, salivary glands, gut, testes, brain, salivary glands, and other sites. Primary ENKTCL of the lung is rare. Here, we report a 68-year-old non-smoking female who presented with fever, dry cough, and night sweats. The chest image showed lung consolidation in the right lower lung field. Pulmonary biopsy showed diffuse abnormal lymphocyte infiltrate in the necrotic exudate. Immunohistochemical data indicated that the tumour...
Source: Journal of Hematopathology - November 15, 2019 Category: Pathology Source Type: research

Survey of ERG expression in normal bone marrow and myeloid neoplasms
AbstractThe immunohistochemical stain ERG is a useful diagnostic marker for leukemia cutis. Translocations involving theERG gene have been identified in acute myeloid leukemia (AML) and it plays critical roles in differentiation of hematopoietic stem cells. However, little is known about ERG expression in the bone marrow or in myeloid neoplasms. The aim of this study is to characterize ERG expression in normal bone marrow specimens, and those with various myeloid neoplasms. We performed immunohistochemical studies assessing ERG expression in bone marrow biopsies obtained over a 1-year period, in which myeloperoxidase (MPO)...
Source: Journal of Hematopathology - November 15, 2019 Category: Pathology Source Type: research

Enteropathy associated T cell lymphoma with Reed-Sternberg-like cells of B cell phenotype and genotype associated with Epstein-Barr virus infection
We describe a case of a 76-year-old Caucasian male who presented to the Emergency Department (ED) with weight loss, abdominal pain, and multiple episodes of vomiting and diarrhea. He underwent a laparotomy with an intraluminal mass seen in the cecum. Histology showed atypical intermediate- to large-sized cells involving the full thickness of the bowel wall with numerous mitotic figures and apoptotic bodies. The adjacent uninvolved mucosa demonstrated villous blunting, increased intraepithelial lymphocytes, crypt elongation, and lamina propria plasmacytosis, consistent with the celiac enteropathy. Rare large transformed cel...
Source: Journal of Hematopathology - November 14, 2019 Category: Pathology Source Type: research

Mummified cells in nodular lymphocyte predominant Hodgkin lymphoma
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - November 12, 2019 Category: Pathology Source Type: research

Primary ovarian diffuse large B-cell lymphoma preceded by autoimmune hemolytic anemia
AbstractA 62-year-old woman was diagnosed with autoimmune hemolytic anemia (AIHA), with cytology- and culture-negative pleural effusion. AIHA did not improve with steroid therapy. Computerized tomography (CT) detected a left ovarian tumor, which was resected surgically, and AIHA improved temporarily. Pathological examination yielded a diagnosis of diffuse large B-cell lymphoma (DLBCL). Positron emission tomography with CT suggested systemic infiltration of lymphoma. Rituximab-containing chemotherapy (R-CHOP regimen) yielded rapid decreases in lymphoma lesions and pleural effusion with improvement of AIHA. After six courses...
Source: Journal of Hematopathology - November 9, 2019 Category: Pathology Source Type: research

Dual antibody immunohistochemistry: an efficient and sensitive tool for the detection of residual disease in chronic lymphocytic leukemia
AbstractHighly effective treatments for chronic lymphocytic leukemia (CLL) have the potential to reduce significant tumor burden to single cells and therefore require sensitive tools to assess for minimal residual disease (MRD) in bone marrow (BM) biopsies. Flow cytometry (FC) is the current gold standard for detection of MRD, but requires a specialized facility, specific antibody panels, collecting hundreds of thousands-to-millions of cells, and personnel with expertise in the analysis and interpretation of FC MRD data, which may not be feasible in many small laboratories. Dual-antibody immunohistochemistry (DA-IHC) can i...
Source: Journal of Hematopathology - November 8, 2019 Category: Pathology Source Type: research

Evolving myelodysplastic syndrome in an HIV patient with history of anal cancer and chemotherapy
AbstractThe incidence of therapy-related myelodysplastic syndromes (t-MDS) has been increasing with the widespread use of highly active antiretroviral therapy (HAART) therapy for HIV and chemotherapy for AIDS-related cancers. The classical dysplastic features in the granulocytes and megakaryocytes may not be easily appreciated. The most reliable distinguishing feature between the hematopoietic dysplasia of t-MDS and that of HIV infection rests on the identification of MDS-related cytogenetic aberrations. Here we report a patient with well-controlled HIV and history of chemotherapy for invasive anal squamous cell carcinoma ...
Source: Journal of Hematopathology - October 30, 2019 Category: Pathology Source Type: research

Classification of malignant lymphoma subtypes in Korean patients: a report of the 4th nationwide study
AbstractTo determine the relative frequency and change of malignant lymphoma in Korea according to the 4th World Health Organization (WHO) classification and compare with previous reports. Between 2015 and 2016, 7737 new patients with malignant lymphoma were enrolled from 31 institutes, with their clinicopathologic information obtained, and evaluated for the relative frequency of lymphoma subtypes. The relative frequency of non-Hodgkin lymphoma (NHL) was 94.8%, and that of Hodgkin lymphoma (HL) was 5.2%. B cell lymphomas accounted for 83.1% of all NHLs; T/natural killer (NK) cell lymphomas, 16.4%; and immunodeficiency-asso...
Source: Journal of Hematopathology - September 6, 2019 Category: Pathology Source Type: research

SOLAHP: a fledgling organization rapidly taking flight
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - August 31, 2019 Category: Pathology Source Type: research

Transition from morphologic diagnosis to immunophenotypic diagnosis of acute leukemia —experience of establishing a new flow cytometry laboratory
This study aimed at documenting the leukemia-associated immunophenotype (LAIP) specific for morphologic (FAB) subclasses of acute leukemia (AL). A retrospective case record-based study was carried out including 100 cases of de novo acute leukemia over 1  year to study the association of FCM immunophenotype profile with morphologic FAB classification of acute leukemia. Fourteen cases (14%) were diagnosed as acute leukemia—unclassified by morphology which were accurately classified by FCM into B cell acute lymphoblastic leukemia (ALL) (6), T cell ALL (3), acute myeloid leukemia (AML) (4), and mixed phenotype acute...
Source: Journal of Hematopathology - August 30, 2019 Category: Pathology Source Type: research

Systemic mastocytosis with renal light chain amyloidosis: associated non-mast cell disorder or concurrent disease
AbstractSystemic mastocytosis with an associated hematological neoplasm (SM-AHN) is a rare diagnosis commonly associated with myeloid disorders. Herein, we describe the case of a 53-year-old female diagnosed with systemic mastocytosis (SM) in conjunction with renal light chain amyloidosis (AL) and smoldering myeloma. Although cytokines such as IL-6 may play a role in the proliferation of plasma cells, delays in the diagnosis of SM and paucity of relevant studies cast uncertainty on whether the associated disease is secondary to the mast cells or etiologically independent. To our knowledge, this is the first case of confirm...
Source: Journal of Hematopathology - August 7, 2019 Category: Pathology Source Type: research

Warthin –Finkeldey-like cells in a lymph node from a child with Li-Fraumeni syndrome
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - August 6, 2019 Category: Pathology Source Type: research

Platelet satellitism: unusual cause of spurious thrombocytopenia
AbstractPlatelet satellitism (PS) is defined as adherence of platelets on the surface of polymorphonuclear leucocytes and imparting a rosette-like appearance especially in ethylenediaminetetraacetic acid (EDTA) whole blood samples. We here present a case of spurious thrombocytopenia resulting from florid platelet adherence on the surface of neutrophils. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - August 1, 2019 Category: Pathology Source Type: research

Post-transplant lymphoproliferative disorder manifesting as lymphomatoid granulomatosis: report of two cases and review of the literature highlighting current challenges in pathologic classification
AbstractClassification of viral- and immunodeficiency-associated lymphoproliferative disorders continues to evolve. Entities in these categories recognized in the 2016 revision of the World Health Organization Classification of Lymphoid Neoplasms show considerable morphologic overlap, and it is unclear whether the current classification conceptualizes the scenarios in which these lesions arise and how they might be best managed. Here, we report two cases of lymphoproliferative disorders meeting histologic criteria for lymphomatoid granulomatosis that arose following solid organ transplant. In reviewing the clinicopathologi...
Source: Journal of Hematopathology - July 27, 2019 Category: Pathology Source Type: research

Inconsistency associated with SOX11 immunohistochemistry in mantle cell lymphoma: a meta-analysis
AbstractSOX11 immunohistochemistry (IHC) in mantle cell lymphoma (MCL) is known to show varied results. Our aim was to evaluate the factor responsible for this variation among different studies. A meta-analysis was performed with the original data including the proportion and number of SOX11-positive MCL cases, host and clonality of SOX11 antibodies, clone or catalog number of antibodies, MCL subtypes, number of cases with indolent traits, number of aggressive variants, and cut-off for SOX11 IHC interpretation. A total of 21 published studies were analyzed. The combined proportion of SOX11-positive MCL cases was 0.80 (95% ...
Source: Journal of Hematopathology - July 22, 2019 Category: Pathology Source Type: research