Secondary plasma cell leukaemia (PCL) with plasmablastic morphology
AbstractA 71-year-old female with relapsed IgA lambda myeloma developed progressive cytopenia. The peripheral blood film showed 5% blastoid cells. Flow cytometry analysis was indicative of plasma cells. The bone marrow smear was packed with plasmablasts. Target CD138-cell FISH and molecular karyotyping identified a complex genome. NGS identified high-risk mutations. Bone marrow histology confirmed myeloma with no evidence of acute leukaemia. The patient was diagnosed with plasmablastic progression of myeloma and secondary PCL. Secondary PCL patients have a poor prognosis. It is essential to recognize this subtype and explo...
Source: Journal of Hematopathology - April 6, 2024 Category: Pathology Source Type: research
In memorium: Dr. Zeba Singh
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - April 5, 2024 Category: Pathology Source Type: research
ALK-positive large B-cell lymphoma (ALK + LBCL) with aberrant CD3 expression
We report an unusual case of ALK1 + LBCL in a 58-year-old man with partial expression of CD3 without other T cell antigen expression. The tissue was evaluated with flow cytometry, immunohistochemistry, fluorescent in situ hybridization, and gene rearrangement studies. Gene rearrangement studies forIGH andTCR gamma were performed. Flow cytometry did not demonstrate any abnormal lymphoid populations. Tissue sectioning shows a malignant plasmacytic large cell neoplasm which expresses CD45 but is negative for CD20, CD79a, and PAX5. Plasmacytic markers CD138 and MUM1 are positive with kappa light chain restriction. Strong g...
Source: Journal of Hematopathology - April 4, 2024 Category: Pathology Source Type: research
Loss of CD5-positive T-cells following anti-thymocyte globulin treatment
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - April 4, 2024 Category: Pathology Source Type: research
Cytogenetic and pathologic characterization of MYC-rearranged B-cell lymphomas in pediatric and young adult patients
AbstractMYC-rearranged B-cell lymphoma (BCL) in the pediatric/young adult (YA) age group differs substantially in disease composition from adult cohorts. However, data regarding the partner genes, concurrent rearrangements, and ultimate diagnoses in these patients is scarce compared to that in adult cohorts. We aimed to characterize the spectrum ofMYC-rearranged (MYC-R) mature, aggressive BCL in the pediatric/YA population. A retrospective study of morphologic, immunophenotypic, and fluorescence in situ hybridization (FISH) results of patients age ≤ 30 years with suspected Burkitt lymphoma (BL), diffuse large B-cel...
Source: Journal of Hematopathology - April 2, 2024 Category: Pathology Source Type: research
Gamma-delta T-cell acute lymphoblastic lymphoma/leukemia: a report of a rare entity
AbstractGamma delta ( γδ) T-cell acute lymphoblastic leukemia/lymphoma (T-ALL) is a rare, aggressive subtype of T-lymphoid leukemia that accounts for only 9–12% of all T-ALL cases. Herein, we report the case of an 8-year-old boy who presented with facial swelling, shortness of breath, and progressive cervical and axi llary lymphadenopathy. Pathological examination, flow cytometry (Navios, Beckman Coulter ClearLLab 10C 10-color T-cell panel [containing FITC-labeled TCR γδ antibody]), chromosomal analysis, interphase FISH, and targeted DNA-based NGS (34-gene Illumina TruSeq Myeloid Panel) were performed. Flow cy tometr...
Source: Journal of Hematopathology - March 25, 2024 Category: Pathology Source Type: research
The first case of six-way complex translocation of t(4;7;9;22;8;14) in a patient with chronic myeloid leukemia
AbstractIn chronic myeloid leukemia (CML), patients exhibit the t(9;22)(q34.1;q11.2) translocation, resulting in the formation of a Philadelphia chromosome (Ph). However, a subset of CML patients display variant complex translocations, characterized by three-way, four-way, and five-way translocations, which have been occasionally associated with a poor prognosis. This case report presents the first case of a t(9;22) variant six-way complex translocation in CML. The R banding chromosome karyotyping technique was used to obtain preliminary karyotyping results, and the multi-probe FISH technique was used to assist in the veri...
Source: Journal of Hematopathology - March 16, 2024 Category: Pathology Source Type: research
In honor of Prof. Dr. Jos é Vassallo, MD, PhD (1957–2024)
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - March 7, 2024 Category: Pathology Source Type: research
Hemophagocytic lymphohistiocytosis caused by herpes simplex virus type 1 in a young adult: a case report with literature review
This study aims to provide a comprehensive overview of the clinical characteristics and treatment outcomes associated with HSV-1-induced HLH. We herein report an adult case of hemophagocytic lymphohistiocytosis caused by herpes simplex virus type 1, diagnosed on the basis of peripheral blood metagenomic next-generation sequencing results. The patient exhibited a favorable response to treatment, involving dexamethasone, intravenous immunoglobulin, and acyclovir. Notably, etoposide administration was deemed unnecessary, and there has been no recurrence of the disease within the year following treatment. Early and sensitive r...
Source: Journal of Hematopathology - February 29, 2024 Category: Pathology Source Type: research
Reviewer thank you
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - February 17, 2024 Category: Pathology Source Type: research
Correction to: Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 4, 2024 Category: Pathology Source Type: research
Initial diagnosis of extranodal NK/T-cell lymphoma in pericardial fluid with concomitant hemophagocytic lymphohistiocytosis (HLH)
We report an unusual case of a 48-year-old male who initially presented with chest pain and shortness of breath. Subsequent diagnosis via pericardial fluid analysis, including flow cytometry and immunohistochemical stains, revealed extranasal NK/T-cell lymphoma without sinonasal involvement. The analysis identified neoplastic lymphoid cells expressing CD2, cytoplasmic CD3, Epstein-Barr virus, and CD56 and exhibiting increased Ki-67 staining. Additionally, the patient developed hemophagocytosis lymphocytosis secondary to NK/T cell lymphoma. Treatment included an interleukin-1 receptor antagonist (anakinra), dexamethasone, r...
Source: Journal of Hematopathology - December 26, 2023 Category: Pathology Source Type: research
The use of leukaemia Q-fusion gene screening assay (Q30) in the diagnostic evaluation of acute myeloid leukaemia (AML)
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - December 26, 2023 Category: Pathology Source Type: research
Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block
AbstractAgarose-based cell block (CB) technique can be modified to be combined with the frozen section technique for the preparation of a high-quality frozen-embedded CB (F-CB) from an effusion or fine-needle aspiration (FNA) cytology sample. This combined technique can be effectively used for the immunocharacterization of the hematolymphoid cells on F-CB. To demonstrate the applicability of performing diagnostic ICC on F-CB, we have analyzed the immunophenotype of the hematolymphoid cells in a series of eight cases of effusions and eight cases of FNA cytology specimens by using CB-ICC on sections cut from frozen-embedded ...
Source: Journal of Hematopathology - December 15, 2023 Category: Pathology Source Type: research
Primary anaplastic large cell lymphoma arising from central nervous system
AbstractA 22-year-old man presented at the emergency department with progressive headache, vomiting and horizontal diplopia over 2-month period. He also developed blurred vision in his left eye. He complained of loss of appetite for the past 2 months, resulting in a 5-kg weight loss. Examination upon arrival revealed papilledema and bilateral abducens nerve palsy. Motor and sensory functions were intact. Magnetic resonance imaging (MRI) of the brain revealed multiple extra-axial nodular enhancing lesions with size of 5 –10 mm mainly along with both sides of falx cerebri and vasogenic brain oedema (Fig.1). Stereotactic br...
Source: Journal of Hematopathology - December 2, 2023 Category: Pathology Source Type: research
