Twenty years later: has cell of origin testing in diffuse large B cell lymphoma run its course?
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - February 28, 2020 Category: Pathology Source Type: research
Molecular spectrum and distribution of hemoglobinopathies in southwest of Iran: a seven-year retrospective study
This study aimed to assess a comprehensive prevalence of most frequent thalassemia mutations in Khuzestan Province, where is a belt of thalassemia in Iran. A total of 6946 subjects were enrolled for evaluating alpha-beta thalassemia from 2012 to 2018. In order to determine the silent mutations, subjects with microcytic hypochromic without anemia with normal Hb, HbA2, and HbF were included too. Genomic DNA was extracted, and ARMS-PCR, Gap-PCR, and DNA sequencing were used to detect thalassemia mutations. Of 6946 individuals, just 880 (12.6%) were normal, and 6066 (87.3%) were the carrier for thalassemia. The most frequent p...
Source: Journal of Hematopathology - February 16, 2020 Category: Pathology Source Type: research
Reviewers for Journal of Hematopathology in 2019
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 26, 2020 Category: Pathology Source Type: research
Mast cell leukemia with t(8;21) and t(12;13): can we classify it as acute mast cell leukemia?
We report the case of a 69-year-old man who presented acute leukemia with circulating blasts and numerous atypical mast cells in peripheral blood. Both blasts and mast cells presented identical cytochemistry (myeloperoxidase-negative, tartrate-resistant acid phosphatase –negative, esterase-negative; toluidine blue–positive; and acid phosphatase–positive). Flow cytometry showed a blast population MPO−, DR−, CD34−, CD117++, CD25+, and CD2−. Bone marrow histology revealed infiltration by CD25+, c-Kit+, and tryptase+ cells. Cytogenetics revealed 46,XY,t(8; 21)(q22; q22),der(12),t(12; 13)(p13; q12-14),del(13)(q14)...
Source: Journal of Hematopathology - January 20, 2020 Category: Pathology Source Type: research
Papular eruption characteristic of persistent agmination of lymphomatoid papulosis
AbstractA 48-year-old man presented with a 1-year history of a nontender, pruritic, well-circumscribed crop of waxing and waning papules. The histological findings were compatible with lymphomatoid papulosis (LyP) in spite of the fact that cutaneous distribution was not typical. Attending to the clinicopathological correlation, we established the diagnosis of persistent agmination of lymphomatoid papulosis (PALP). PALP is a rare variant of CD30+ primary cutaneous T cell lymphoproliferative disorder, with only a few reported cases in the literature. It is still controversial whether PALP is a subtype of localized LyP or an ...
Source: Journal of Hematopathology - January 19, 2020 Category: Pathology Source Type: research
Mantle cell lymphoma mimicking chronic lymphocytic leukemia/small lymphocytic lymphoma on flow cytometry
AbstractOne of the most common applications of clinical flow cytometry is to diagnose chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). When classic features are present and correlation with other diagnostic modalities is possible, the distinction of CLL/SLL from other subtypes of B-cell lymphoma is straightforward. However, features deviating from prototypical phenotypes occur not infrequently and can present a diagnostic dilemma. One potential pitfall that has not been well described is mimicry of CLL/SLL by mantle cell lymphoma. A review of flow cytometric data and corresponding clinical, morphologic, a...
Source: Journal of Hematopathology - January 17, 2020 Category: Pathology Source Type: research
CD56-positive B cell precursor acute lymphoblastic leukemia harboring KMT2A-AFF1 rearrangement developed in a pregnant woman successfully treated with allogeneic hematopoietic cell transplantation
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 6, 2020 Category: Pathology Source Type: research
Decreased activity of plasma ADAMTS-13 predicts poor prognosis in acute lymphoblastic leukemia patients after hematopoietic stem cell transplant
AbstractDecreased ADAMTS-13 is important for the diagnosis of thrombotic thrombocytopenic purpura (TTP), but recent studies have shown that reduced ADAMTS-13 activity is associated with disease prognosis of a variety of cancer patients. As an important hematological malignancy, the significance of ADAMTS-13 in acute lymphoblastic leukemia (ALL) has not been fully elucidated. This research aims to explore the significance of decreased ADAMTS-13 activity in acute lymphoblastic leukemia after hematopoietic stem cell transplant (HSCT). Thirty-eight ALL patients were included in this research, and their ADAMTS-13 activity was m...
Source: Journal of Hematopathology - January 2, 2020 Category: Pathology Source Type: research
Hyperleucocytosis grading score and NPM1 gene mutation among patients with acute myeloid leukemia: Malaysian experience
AbstractThis is the first study evaluating the frequencies of nucleophosmin (NPM1) gene mutation according to the hyperleukocytosis (HL) grading score. The current two cut off values of this score in acute myeloid leukaemia (AML) patients are [1] those with 50 –100 × 109/L and [2] those with ≥ 100 × 109/L total WBC count. The score represents the current clinically based definition of HL in patients with AML. A total of 90 patients with AML were included. AML patients were stratified into three groups: [1] those with a WBC count below 50  × 109/L (n = 33), [2] those with a WBC count 50–100 ×â€...
Source: Journal of Hematopathology - January 2, 2020 Category: Pathology Source Type: research
Multiplexing 8 colors with 12 antibodies in a single lymphoid screening tube by flow cytometry for evaluating suspected chronic lymphoproliferative disorders (CLPD)
AbstractThe diagnosis of chronic lymphoproliferative disorder (CLPD) or non-Hodgkin Lymphoma (NHL) is based on the detection of the abnormal clonal lymphoid cells. The flow cytometry (FCM) immunophenotyping not only plays an essential role in the screening of CLPD but also helps in the specific identification and characterization of the expanded aberrant lymphocytes. Over decades, it has evolved from a single parameter to multi-parameter assessment by 3- to 12-color FCM. The greatest challenge is to characterize abnormal lymphoid cells by a limited immunophenotype (IPT) panel. A study was undertaken to evaluate the diagnos...
Source: Journal of Hematopathology - December 1, 2019 Category: Pathology Source Type: research
A case of an unusual lineage switch in late relapse ALL —is it actually a secondary leukemia?
AbstractAcute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid precursors. According to immunophenotype, it is further subdivided into precursor B cell ALL and precursor T cell ALL, with precursor B cell ALL being much more common both in children and adults. Lineage switch from one lymphoid lineage to another during the course of the disease is extremely rarely reported. Here, we describe a case of a child who initially presented as a precursor B-ALL but 15 Â years later and after two successfully treated relapses of the original ALL presented with early T cell precursor leukemia. Although it was considered...
Source: Journal of Hematopathology - November 19, 2019 Category: Pathology Source Type: research
Primary pulmonary extranodal NK/T cell lymphoma of an elderly adult: a case report and literature review
AbstractExtranodal natural killer/T-cell lymphoma (ENKTCL), nasal type, is rare and aggressive and often involves the nose, nasopharynx, and upper aerodigestive tract. The non-nasal type can affect the skin, salivary glands, gut, testes, brain, salivary glands, and other sites. Primary ENKTCL of the lung is rare. Here, we report a 68-year-old non-smoking female who presented with fever, dry cough, and night sweats. The chest image showed lung consolidation in the right lower lung field. Pulmonary biopsy showed diffuse abnormal lymphocyte infiltrate in the necrotic exudate. Immunohistochemical data indicated that the tumour...
Source: Journal of Hematopathology - November 14, 2019 Category: Pathology Source Type: research
Survey of ERG expression in normal bone marrow and myeloid neoplasms
AbstractThe immunohistochemical stain ERG is a useful diagnostic marker for leukemia cutis. Translocations involving theERG gene have been identified in acute myeloid leukemia (AML) and it plays critical roles in differentiation of hematopoietic stem cells. However, little is known about ERG expression in the bone marrow or in myeloid neoplasms. The aim of this study is to characterize ERG expression in normal bone marrow specimens, and those with various myeloid neoplasms. We performed immunohistochemical studies assessing ERG expression in bone marrow biopsies obtained over a 1-year period, in which myeloperoxidase (MPO)...
Source: Journal of Hematopathology - November 14, 2019 Category: Pathology Source Type: research
Enteropathy associated T cell lymphoma with Reed-Sternberg-like cells of B cell phenotype and genotype associated with Epstein-Barr virus infection
We describe a case of a 76-year-old Caucasian male who presented to the Emergency Department (ED) with weight loss, abdominal pain, and multiple episodes of vomiting and diarrhea. He underwent a laparotomy with an intraluminal mass seen in the cecum. Histology showed atypical intermediate- to large-sized cells involving the full thickness of the bowel wall with numerous mitotic figures and apoptotic bodies. The adjacent uninvolved mucosa demonstrated villous blunting, increased intraepithelial lymphocytes, crypt elongation, and lamina propria plasmacytosis, consistent with the celiac enteropathy. Rare large transformed cel...
Source: Journal of Hematopathology - November 13, 2019 Category: Pathology Source Type: research
Mummified cells in nodular lymphocyte predominant Hodgkin lymphoma
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - November 11, 2019 Category: Pathology Source Type: research
