Classical and molecular methods in differentiation of mantle cell lymphoma and small lymphocytic lymphoma in composite lymphoma: a case report
This report presents the possibility of determining clonality of each CL component by using flow cytometry in parallel with specific molecular markers. Molecular analysis was performed by quantitative real-time PCR (qPCR) using two markers:IGH rearrangement as reported by van Dongen et al. (Leukemia 2003;17(12):2257-317) andSOX11 expression by Hamborg et al. (Eur J Haematol 2012;89(5):385-94) and Szostakowska et al. (Med Oncol 2018;35(4):49). TheSOX11 expression allowed us to distinguish MCL and SLL subclones in a 60-year-old male with multifocal MCL/SLL as this marker is not detected in SLL as reported by Swerdlow et al. ...
Source: Journal of Hematopathology - January 7, 2021 Category: Pathology Source Type: research
An unusual case of lymphoplasmacytic lymphoma/Waldenstr öm macroglobulinemia presenting with intractable seizures and interference with automated testing
AbstractLymphoplasmacytic lymphoma (LPL) is a B cell neoplasm composed of small lymphocytes, plasmacytoid lymphocytes, and plasma cells, which typically involves the bone marrow. Most cases of LPL present with Waldenstrom macroglobulinemia (WM) defined as the presence of monoclonal immunoglobulins of IgM type and LPL in the bone marrow. Herein, we present a case of LPL/WM presenting with intractable refractory seizures. A 71-year-old man with a 5-month history of seizures was brought to the emergency room after being found unresponsive. Automated hematology analysis revealed imprecise counts, wide variation, and multiple e...
Source: Journal of Hematopathology - January 7, 2021 Category: Pathology Source Type: research
A multi-centre case series of alectinib-related erythrocyte membrane changes and associated haemolysis
AbstractAlectinib is an orally bioavailable anaplastic lymphoma kinase (ALK) inhibitor indicated forALK mutated non-small cell lung cancer (NSCLC). This case series documents the development of significant erythrocyte membrane changes associated with alectinib use in six patients. Morphological findings found on blood film examination include moderate-marked acanthocytes, spheroacanthocytes, and one case demonstrated moderate schistocytes. Two patients in this multi-centre case series developed grade 1 anaemia, and four patients developed grade 2 anaemia. Two patients suffered significant non-immune-related haemolysis. One...
Source: Journal of Hematopathology - January 7, 2021 Category: Pathology Source Type: research
An unpublished CEBPA mutation in familial leukemia
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 7, 2021 Category: Pathology Source Type: research
Progress in the application of minimal residual disease detection in multiple myeloma
AbstractCurrently, multiple myeloma is an incurable malignant plasma cell neoplasm, and the incidence rate ranks second in terms of hematological malignancies. Most of the patients are diagnosed at an age between 65 and 74 years, with a median age of 69 years. After the introduction of novel drugs or therapeutic methods, the treatment efficacy became significantly improved in multiple myeloma patients. Minimal residual disease is considered to be the most important reason for relapse in multiple myeloma patients after complete remission, which is significantly related to progression-free survival and overall survival. Ther...
Source: Journal of Hematopathology - January 4, 2021 Category: Pathology Source Type: research
Erythroblastic sarcoma transformation from a chronic myeloid neoplasm with FGFR1 rearrangement presenting as a pleural effusion: a case report
AbstractMyeloid/lymphoid neoplasms withFGFR1 rearrangement (MLN-FGFR1) comprise a heterogeneous group of neoplasms that can present as a myeloproliferative neoplasm, a myelodysplastic/myeloproliferative neoplasm, acute myeloid leukemia, T or B lymphoblastic leukemia/lymphoma, or mixed phenotype acute leukemia. The overall outcome of these neoplasms is poor, and these patients often show cytogenetic evolution with gain of additional chromosomal abnormalities leading to transformation to acute leukemia. Pure erythroid leukemia and erythroblastic sarcoma evolving from a preexisting chronic myeloid neoplasm have rarely been de...
Source: Journal of Hematopathology - January 3, 2021 Category: Pathology Source Type: research
Evaluation of the role of CD47 in sickle cell disease
This study aimed to examine the expression of CD47 on RBCs and SIRPα on PBMC cells in SCD patients and the apoptosis of SCD RBCs. We also measured the l evels of pro-inflammatory cytokines in SCD patients and correlated it with the cell surface marker expression of CD47 and SIRPα to determine whether CD47 and/or SIRPα played a role in promoting the pro-inflammatory phenotype in SCD. Whole blood samples were drawn from SCD patients, and healthy con trol and PBMC were isolated and stained with SIRPα. Change in CD47, apoptosis by annexin V marker, and pro-inflammatory cytokines were measured and correlation among these va...
Source: Journal of Hematopathology - January 2, 2021 Category: Pathology Source Type: research
Lymphoplasmacytic lymphoma and a CD5+ lymphoproliferative process presenting as composite disease in the bone marrow: a report of two cases
AbstractCorrect subclassification of B cell lymphoma requires integration of histologic and immunohistochemical findings in addition to results of flow cytometric immunophenotyping, cytogenetic analysis, and molecular studies. Evaluation for the MYD88 L265P mutation and immunohistochemical staining for LEF1 can be helpful in the workup of small B cell lymphomas. In this case report of two diagnostically difficult cases, we present two patients with lymphoplasmacytic lymphoma (LPL) and a CD5+ lymphoproliferative process presenting as composite disease within the bone marrow. In both cases, integration of ancillary studies a...
Source: Journal of Hematopathology - January 2, 2021 Category: Pathology Source Type: research
Erdheim Chester disease presenting as sclerosing mesenteritis
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - January 2, 2021 Category: Pathology Source Type: research
Ocular adnexal lymphoma: long-term outcome, patterns of failure and prognostic factors in 174 patients
AbstractDetailed information regarding follow-up of ocular adnexal lymphoma and of the frequency of large cell transformation of low-grade ocular adnexal lymphoma is limited. We studied 174 patients with ocular adnexal lymphoma (OAL) to evaluate long-term clinical outcome and patterns of failure. All lymphomas presenting with involvement of the ocular adnexa diagnosed at the Massachusetts General Hospital (MGH) between 1974 and 2007, in which at least 6 months of follow-up was available, were included in this study. There were 106 extranodal marginal zone lymphomas (MALT lymphomas, MZL), 40 follicular lymphomas (FL), 16 di...
Source: Journal of Hematopathology - November 7, 2020 Category: Pathology Source Type: research
Computational histopathology and deep transfer learning: characterizing the molecular basis of tumor morphology
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - November 5, 2020 Category: Pathology Source Type: research
Case report: a fatal combination of hemophagocytic lymphohistiocytosis with extensive pulmonary microvascular damage in COVID-19 pneumonia
AbstractThe clinical features of COVID-19 have a considerable range from a mild illness to severe disease. Underlying pathophysiological mechanisms of the rapidly progressive, and often fatal, pulmonary disease frequently observed in COVID-19 need to be elucidated, in order to develop new treatment strategies for different disease endotypes. Fatal cases can display features of a cytokine storm, which may be related to hemophagocytic lymphohistiocytosis. Also, a spectrum of vascular changes, including microvascular damage, is known to accompany severe COVID-19. In this paper, we describe the co-occurrence of hemophagocytic ...
Source: Journal of Hematopathology - October 23, 2020 Category: Pathology Source Type: research
Utility of hyperchromic cell percentage, flags, and red cell cytograms generated by Advia-120 hematology analyzer as a potential screening tool in hereditary spherocytosis
AbstractHereditary spherocytosis (HS) is the commonest inherited red cell membrane disorder seen in clinical practice. Modern hematology analyzers generate newer parameters that can help in screening of HS. The present study focuses on the utility of red blood cell data generated by Advia-120 hematology analyzer as a screening tool for patients with HS. Blood samples from 21 patients with HS were analyzed on Advia-120 (Technicon H*1 series) analyzer. The percentage of hyperchromic cells and hyperchromic and hemoglobin concentration variance flags along with the RBC cytogram were studied. A control group of 50 healthy indiv...
Source: Journal of Hematopathology - October 19, 2020 Category: Pathology Source Type: research
Low class –switched memory B cells predict the need for continued immunoglobulin replacement following B cell reconstitution after rituximab: a case series and review of the literature
ConclusionThe low class-switched memory B cells (CD27+IgM −IgD−) may serve as a potential biomarker for the need of continued immunoglobulin replacement therapies. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - October 17, 2020 Category: Pathology Source Type: research
Acquired Glanzmann thrombasthenia: a rare disorder
We describe a patient of ITP with borderline low platelet count and severe bleeding, who posed a diagnostic challenge. However, an accurate diagnosis and suitable management helped to avoid catastrophic bleeding. (Source: Journal of Hematopathology)
Source: Journal of Hematopathology - October 16, 2020 Category: Pathology Source Type: research
