Activation of cytotoxic T cell population and inversion of CD4:CD8 ratio as manifestations of cellular immune response in SARS-COV-2 infection
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - July 1, 2020 Category: Pathology Source Type: research
A practical approach to FISH testing for MYC rearrangements and brief review of MYC in aggressive B-cell lymphomas
AbstractAggressive B-cell lymphomas, including the WHO diagnoses of diffuse large B-cell lymphoma, high-grade B-cell lymphoma withMYC andBCL2 and/orBCL6 rearrangements, high-grade B-cell lymphoma, not otherwise specified, and Burkitt lymphoma, together account for approximately 40% of B-cell non-Hodgkin lymphomas. Identification ofMYC,BCL2, andBCL6 rearrangements in these neoplasms is critical for diagnostic, prognostic, and therapeutic purposes. Herein, we address technical issues surrounding identification of these genetic abnormalities, discuss their diagnostic, prognostic, and therapeutic implications, and present an a...
Source: Journal of Hematopathology - June 17, 2020 Category: Pathology Source Type: research
Pediatric post-transplant lymphoproliferative disorder identified on random gastrointestinal biopsies shows non-mass-forming PTLD with clonal plasma cells: report of 2 cases
AbstractEBV-related lymphoproliferations in post-transplant settings range from non-destructive hyperplasia to polymorphic post-transplant lymphoproliferative disorder (PTLD) to overt lymphoma. Here we present two unusual cases of pediatric non-mass-forming gastrointestinal (GI) PTLDs with mixed pathologic features of non-destructive and polymorphic categories. Both patients were EBV na ïve and presented within 1 year of transplant with non-specific gastrointestinal symptoms (diarrhea, abdominal pain, and food intolerance) with hypoalbuminemia, cytopenia, and EBV viremia. No mass lesions were found on imaging and endosco...
Source: Journal of Hematopathology - June 11, 2020 Category: Pathology Source Type: research
Mucosal intralymphatic spread in a relapsed diffuse large B cell lymphoma
AbstractIntralymphatic spread is a rare finding and is associated with poor prognosis in diffuse large B cell lymphoma (DLBCL). Here, we report a case of relapsed DLBCL with mucosal intralymphatic spread. A 69-year-old man had been diagnosed with gastric DLBCL stage IIE at 57 years. He had a relapse with generalized lymphadenopathy and extranodal involvement at 61 years; then, second complete remission was achieved after salvage chemotherapy. He then had a second relapse with involvement of the terminal ileum, spinal cord, and left tonsil. The terminal ileum showed in tralymphatic spread in the lamina propria of the int...
Source: Journal of Hematopathology - June 11, 2020 Category: Pathology Source Type: research
Transformation of de novo high-grade B cell lymphoma with MYC and BCL2 rearrangements to double-hit B lymphoblastic leukemia/lymphoma: a case report and review of literature
AbstractHigh-grade B cell lymphomas withMYC andBCL2 and/orBCL6 rearrangements ( “double-hit” lymphoma (DHL)/“triple-hit” lymphoma (THL)) are mature B cell neoplasms with highly aggressive clinical behavior and poor response to therapy. Lymphoblastic transformation of mature B cell neoplasms is an uncommon event that is best recognized for follicular lymphoma (FL). To ou r knowledge, only one case of “B lymphoblastic” transformation of de novo DHL has been reported in the literature. Here, we describe another case of such transformation. The patient was a 57-year-old man who presented with hypercalcemia, high la...
Source: Journal of Hematopathology - June 5, 2020 Category: Pathology Source Type: research
Mantle cell lymphoma: pathologic and immunophenotyping study of 50 cases and correlation with survival —a single institute experience
This study focuses on (1) age, gender, and location of tumor at presentation; (2) some challenges in the diagnostic criteria; (3) cytomorphology, histomorphology, immunophenotyping, and proliferation rate; and (4) overall survival and finally relationship with demographic and pathologic findings which shows no association between survival of patients and gender, cytomorphology, histology, and location of tumor while increased mitotic activity and age are prognostically important factors. Cyclin D1 immunostaining is strictly recommended in all low-grade B cell lymphoma due to absence of CD5 expression on 20% of confirmed MC...
Source: Journal of Hematopathology - May 28, 2020 Category: Pathology Source Type: research
The molecular analysis of four coexistent mutations in additional sex combs like 1 (ASXL1) gene in a patient with acute myeloid leukemia
AbstractMutations in the additional sex combs like 1 (ASXL1) gene are frequently involved in clonal hematopoiesis and are connected with an increased risk of hematologic cancer. These aberrations are frequently detected in a number of different hematological neoplasms including acute myeloid leukemia (AML). Patients harboringASXL1 mutations tend to have a poor prognosis and poor response to therapy. Here, we report the coexistence of four different mutations inASXL1 gene in a patient with AML. Such accumulation of mutations, in this gene, has not been described yet. Our findings suggest that accumulations of mutations inAS...
Source: Journal of Hematopathology - May 26, 2020 Category: Pathology Source Type: research
Osteopetrosis in a six-month-old infant
We report a six-month-old male with osteopetrosis where bone marrow examination and molecular studies allowed a definitive diagnosis. Microscopic examination of a bone marrow biopsy demonstrated increased bone formation at various stages of ossification, decreased marrow spaces, and increased osteoclasts rimming the bone. Next-generation sequencing (NGS) demonstrated that the patient was homozygous for mutations in theTCIRG1 gene, which encodes an osteoclast vacuolar proton pump and is the most common mutation associated with the autosomal recessive or infantile malignant form of osteopetrosis. The patient underwent a cord...
Source: Journal of Hematopathology - May 26, 2020 Category: Pathology Source Type: research
COVID-19 and remembering Professor Dennis H. Wright
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - May 25, 2020 Category: Pathology Source Type: research
Pacinian corpuscle in human lymph node: a report and the literature review
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - May 21, 2020 Category: Pathology Source Type: research
Reticulocyte count and its parameters: comparison of automated analyzers, flow cytometry, and manual method
In this study, we evaluated the three methods of reticulocyte counting, namely automated, flow cytometry, and manual methods for reticulocyte count and also for the enumeration of immature reticulocyte fraction (IRF). Reticulocyte count was done by manual method (light microscopy); two different automated analyzers, LH-780, Beckman Coulter, USA and Pentra-XLR, Horiba Ltd., Japan; and flow cytometry (FACS Canto II, B.D. Bioscience, USA). Also, the classification of stages of maturation was done, and percentage was counted based on quantity of reticulum and its distribution in the cytoplasm. Statistical analysis was done to ...
Source: Journal of Hematopathology - May 20, 2020 Category: Pathology Source Type: research
Enumeration of CD34+ blasts by immunohistochemistry in bone marrow biopsies from MDS patients may have significant impact on final WHO classification
AbstractThe percentage of blasts cells in the bone marrow (BM) of MDS patients is one of the key parameters for MDS classification and for the differential diagnosis with acute myeloid leukemia (AML). Currently, the gold standard to determine the blast percentage is conventional cytomorphology. To assess the possible impact of blast cell enumeration in BM biopsies from MDS patients on the final WHO classification using CD34 immunohistochemistry (IHC) a total of 156 BM samples from MDS and MDS-AML patients were studied and compared to blast counts by cytomorphology (CM). Eighty-nine BM aspirates were also studied by flow ...
Source: Journal of Hematopathology - April 22, 2020 Category: Pathology Source Type: research
Peripheral T cell lymphoma, NOS with aberrant αβ and γδ T cell receptor expression in a post-heart transplant patient
AbstractThree main patterns of T cell receptor (TCR) expression are identified in peripheral T cell lymphoma (PTCL): TCR- αβ type, TCR-γδ type, and TCR silent type. Cases of PTCL that express both αβ and γδ receptors are rare. Here, we report a case of PTCL-NOS (not otherwise specified) with dual αβ and γδ expression in a 61-year-old female 6 years after orthotopic cardiac transplant. The patient presented with fatigue, fever, worsening anemia, and thrombocytopenia. A chest CT showed multiple irregular lung nodules and diffuse lymphadenopathy. Cytology evaluation of the lymph nodes revealed large atypical lymp...
Source: Journal of Hematopathology - April 1, 2020 Category: Pathology Source Type: research
Immunophenotypic and molecular analysis of a B cell lymphoma with discordant light chain expression at different anatomic sites
We report a case of a 75-year-old woman with a high-grade B cell lymphoma with MYC and BCL2 rearrangements (HGBCL) with discordant immunoglobulin light chain restriction between a lymph node and a pleural fluid involved by lymphoma. Cytogenetic studies by FISH showed MYC and BCL2 rearrangement in both specimens. Immunoglobulin heavy chain and kappa light chain analysis demonstrated similar rearranged clonal peaks in the two specimens. A review of the literature revealed eight cases of B cell lymphoma with discordant immunoglobulin light chain expression (including our case). The light chain discrepancy occurred during a re...
Source: Journal of Hematopathology - March 9, 2020 Category: Pathology Source Type: research
Does the order of mutational acquisition in myeloproliferative neoplasms matter? Evidence from JAK2 exon 12 and DNMT3A co-mutant polycythemia vera
(Source: Journal of Hematopathology)
Source: Journal of Hematopathology - March 4, 2020 Category: Pathology Source Type: research
