Epstein-Barr Virus EBNA-2 Polymorphic Patterns in Nasopharyngeal Carcinoma in Southern China
Conclusions: E2-A is the predominant subtype in Southern China, and the distribution of EBNA-2 subtypes between NPCs and TWs is significantly different. In Southern China, the E2-A subtype is significantly more frequent in NPCs than TWs, and is also more common than in Northern China. This suggests that the E2-A subtype may play an important role in the pathogenesis of NPC and that EBNA-2 gene variations are tumor-specific polymorphisms.Intervirology 2015;58:386-392 (Source: Intervirology)
Source: Intervirology - April 12, 2016 Category: Virology Source Type: research
Cutaneous Human Polyomavirus Small T Antigens and 4E-BP1 Targeting
Merkel cell polyomavirus (MCPyV), trichodysplasia spinulosa-associated polyomavirus (TSPyV), human polyomavirus 6 (HPyV6), and human polyomavirus 7 (HPyV7) are implicated in the pathogeneses of distinct hyperproliferative cutaneous growths and encode small tumor (sT) antigens. The current study demonstrates that the four sT antigens differentially regulate 4E-binding protein 1 (4E-BP1) serine 65 hyperphosphorylation. MCPyV and HPyV7 sT antigens were found to promote the presence of the hyperphosphorylated 4E-BP1-δ isoform, while TSPyV and HPyV6 sT antigens had no significant effects. Given that hyperphosphorylated 4E-BP1 ...
Source: Intervirology - April 7, 2016 Category: Virology Source Type: research
Influence of Host and Viral Factors on Patients with Chronic Hepatitis C Virus Genotype 6 Treated with Pegylated Interferon and Ribavirin: A Systematic Review and Meta-Analysis
Conclusions: Treatment outcomes for HCV-6 patients are superior to those for HCV-1 patients and comparable to those of HCV-2 and HCV-3 patients, especially at 48 weeks. The level of fibrosis affects treatment outcome, but SVR rates are not significantly different between genders. IL28B and IFNL4 polymorphisms are not significantly associated with HCV-6 treatment outcome.Intervirology 2015;58:373-381 (Source: Intervirology)
Source: Intervirology - March 25, 2016 Category: Virology Source Type: research
Subclinical Tick-Borne Encephalitis Virus in Experimentally Infected Apodemus agrarius
Conclusion: Our work shows the first evidence that subclinical TBEV encephalitis may occur in Apodemus species, depending on the virus load of the inoculum. The antiviral response of the local innate immune system may influence the resistance of Apodemus individuals to lower infectious doses. Per oral/nasal infection seems to be more dangerous for the host than other routes of infection.Intervirology 2015;58:369-372 (Source: Intervirology)
Source: Intervirology - March 2, 2016 Category: Virology Source Type: research
Recombinant VLP-Z of JC Polyomavirus: A Novel Vector for Targeting Gene Delivery
This study tested the feasibility of using a modified VLP with a IgG binding domain (Z domain) of protein A in its capsid for targeted gene delivery. The Z domain of protein A isolated from the membrane of Staphylococcus aureus was inserted into the NH3-terminus of VP1, the major JCPyV capsular protein. The recombinant VLP-Z was produced using Escherichia coli. Electron-microscopic analysis showed that VLP-Z has a viral-like structure. A hemagglutination test showed that VLP-Z has hemagglutination activity. VP1 was detected in the nuclei of HeLa cells by immunostaining after VLP-Z inoculation, suggesting that VLP-Z is viab...
Source: Intervirology - February 24, 2016 Category: Virology Source Type: research
Stromal Cell-Derived Factor-1-3′A Polymorphism Favors HIV-1 Infection in Mexican Women
Conclusion: Here we describe the association of the SDF1-3′A polymorphism with HIV-1 infection only in women, but not to CD4+ T-lymphocyte categories, viral load levels in patients with HIV-1/AIDS, or to exposure levels in female sex workers.Intervirology 2015;58:357-362 (Source: Intervirology)
Source: Intervirology - February 11, 2016 Category: Virology Source Type: research
Clinical Significance of Viral Genome Persistence in the Myocardium of Patients with Dilated Cardiomyopathy
Conclusions: High prevalence of a viral genome in the myocardium of patients with DCM did not have an influence on their long-term clinical outcome.Intervirology 2015;58:350-356 (Source: Intervirology)
Source: Intervirology - February 4, 2016 Category: Virology Source Type: research
Recombinant Mouse β-Defensin 3 Protects against Coxsackievirus B3-Induced Myocarditis in Mice
Conclusion: rMBD3 was demonstrated to possess anti-CVB3 activity in vivo and in vitro.Intervirology 2015;58:343-349 (Source: Intervirology)
Source: Intervirology - February 1, 2016 Category: Virology Source Type: research
Chikungunya Virus Infection Alters Expression of MicroRNAs Involved in Cellular Proliferation, Immune Response and Apoptosis
Conclusion(s): We identified several miRNAs that may play important roles in early events after CHIKV infection and can be potential therapeutic targets against CHIKV infection.Intervirology 2015;58:332-341 (Source: Intervirology)
Source: Intervirology - February 1, 2016 Category: Virology Source Type: research
Detection of Human Papillomavirus Infections at the Single-Cell Level
Conclusion: Given the heterogeneity of HPV status in individual cells, further clarification of HPV infection at the single-cell level may refine our understanding of HPV-related carcinogenesis.Intervirology 2015;58:324-331 (Source: Intervirology)
Source: Intervirology - January 28, 2016 Category: Virology Source Type: research
Detection of the Assembly and Disassembly of PCV2b Virus-Like Particles Using Fluorescence Spectroscopy Analysis
Monitoring the assembly and disassembly of virus-like particles (VLPs) is important in developing effective VLP-based vaccines. We tried to establish a simple and rapid method to evaluate the status of VLP assembly using fluorescence spectroscopic analysis (FSA) while developing a VLP-based vaccine against porcine circovirus type 2b (PCV2b). We synthesized the gene coding for PCV2b capsid protein (CP). The CP was expressed in Escherichia coli in a soluble form, dialyzed into three different buffers, and assembled into VLPs. The immunogenicity of the VLPs was evaluated by an enzyme-linked immunosorbent assay using the sera ...
Source: Intervirology - January 20, 2016 Category: Virology Source Type: research
Is Anti-Hepatitis C Virus Antibody Level an Appropriate Marker to Preclude the Need for Supplemental Testing
Conclusions: We determined that very low anti-HCV levels (S/Co ≤3.0), as determined by chemiluminescence immunoassay, was not an appropriate marker to preclude the need for supplemental testing in our study population. A screening strategy employing a secondary HCV antibody assay using different HCV antigens from the first assay as the supplemental testing method should be studied further. The immunoblot assay, as a supplemental testing method, is still necessary.Intervirology 2015;58:310-317 (Source: Intervirology)
Source: Intervirology - January 20, 2016 Category: Virology Source Type: research
Mutations in the Leucine Zipper-Like Motif of the Human Parainfluenza Virus 3 Fusion Protein Impair Fusion Activity
Conclusions: This motif has an important influence on fusion activity, and its integrality is indispensable for membrane fusion.Intervirology 2015;58:297-309 (Source: Intervirology)
Source: Intervirology - December 23, 2015 Category: Virology Source Type: research
Effect of Nonstructural Protein 2 Hypervariable Regions in the Replication of Porcine Reproductive and Respiratory Syndrome Virus in Marc-145 Cells
Conclusions: The 120 aa from Nsp2, especially aa 628-727, play a negative role in PRRSV TJM proliferation.Intervirology 2015;58:288-296 (Source: Intervirology)
Source: Intervirology - December 23, 2015 Category: Virology Source Type: research
Hepatitis E Virus Seroprevalence Rate in HIV-Infected Patients in Germany: A Comparison of Two Commercial Assays
Conclusions: About one quarter of HIV-infected patients show evidence of previous HEV contact. The risk of developing chronic HEV infection is very low in individuals receiving appropriate antiretroviral therapy. The large variability in HEV seroprevalence rates determined by different assays requires consideration for the diagnostic workup of HIV patients.Intervirology 2015;58:283-287 (Source: Intervirology)
Source: Intervirology - December 1, 2015 Category: Virology Source Type: research
