Evaluation of Temporal Virological Responses to Interferon- α-2b plus Ribavirin among Genotype 3a Hepatitis C Virus-Infected Patients
Conclusions: Individuals incompliant with their treatment who have a higher RVR significantly influence their SVR towards a better remission that can be treated within a short duration with standard treatment.Intervirology (Source: Intervirology)
Source: Intervirology - November 15, 2017 Category: Virology Source Type: research
No Correlation of the Disease Severity of Influenza A Virus Infection with the rs12252 Polymorphism of the Interferon-Induced Transmembrane Protein 3 Gene
The 2009 H1N1 influenza pandemic, which involved a more pathogenic virus than seasonal influenza viruses, rapidly spread around the world and caused many deaths in humans. The members of the interferon-induced transmembrane (IFITM) protein family prevent viral replication and are crucial for defending the host cell against influenza A virus (IAV). Several studies suggest that the CC genotype at the single nucleotide polymorphism (SNP) rs12252 ofIFITM3 confers a genetic predisposition to pandemic influenza A in Europeans and Han Chinese, although one study in a British cohort failed to show an association. In order to exami...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection
Infection of human MRC-5 cells and mouse NIH-3T3 cells with a murine gamma-herpesvirus (MuHV-4 strain 68; MHV-68) photoinactivated by visible light in the presence of methylene blue (MB) resulted in nonproductive infection and the appearance of morphologically transformed cells. Two stably transformed cell lines were derived from both of these cell types and were confirmed to contain both viral DNA and antigen. Next, a quiescent MHV-68 infection in MRC-5 and NIH-3T3 cells was established after cultivation at 41 °C in the presence of phosphonoacetic acid. Following the exposure of quiescently infected cells to visible ligh...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Detection of Merkel Cell Polyomavirus in Respiratory Tract Specimens
Conclusions: MCPyV was observed in specimens from patients with acute respiratory diseases, indicating that there may be a relationship between the virus and these diseases. We were not able to detect MCPyV in samples from patients with chronic lung diseases, including lung cancer, suggesting no association with MCPyV infection and no involvement of this polyomavirus in lung cancerogenesis.Intervirology 2017;60:28-32 (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A
Conclusion: There is a direct correlation of quantities of syncytia and inoculum size, but not with mRNA levels of HRSV-A F protein. Importantly, levels of F protein mRNA were directly related to progeny production.Intervirology 2017;60:56-60 (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Identifying Attenuating Mutations: Tools for a New Vaccine Design against < b > < i > Flaviviruses < /i > < /b >
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
The Histopathological Characteristics Caused by < b > < i > Trionyx sinensis < /i > < /b > Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles ( < b > < i > Pelodiscus sinensis < /i > < /b > )
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Concurrent Infection with < b > < i > Plasmodium vivax < /i > < /b > and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR
Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens
Conclusions: The formulation tests showed that a combination of 0.02 μg of HBs, 0.2 μg of preS1-preS2-HBs, and 0.02 μg of HBc was effective in eliciting specific antibodies in mice.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Phylogenetic Analysis of Torque Teno Virus in Thalassemic Children in Egypt
Conclusion: TTV is highly prevalent among children with thalassemia in Egypt, with a relatively high infection rate also detected among healthy children.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research
Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens
Conclusions: The formulation tests showed that a combination of 0.02 μg of HBs, 0.2 μg of preS1-preS2-HBs, and 0.02 μg of HBc was effective in eliciting specific antibodies in mice.Intervirology (Source: Intervirology)
Source: Intervirology - September 28, 2017 Category: Virology Source Type: research
Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR
Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.Intervirology (Source: Intervirology)
Source: Intervirology - September 27, 2017 Category: Virology Source Type: research
Concurrent Infection with Plasmodium vivax and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - September 15, 2017 Category: Virology Source Type: research
