Evaluation of the Automated QIAsymphony SP/AS Workflow for Cytomegalovirus DNA Extraction and Amplification from Dried Blood Spots
Conclusions: We show that the QIAsymphony SP/AS and RotorGene Q workflow is suitable for CMV DNA extraction and detection from DBSs and that the system correctly identified newborns at risk of late sequelae due to CMV infection.Intervirology 2016;59:211-216 (Source: Intervirology)
Source: Intervirology - March 9, 2017 Category: Virology Source Type: research
Prevalence of Occult Hepatitis C Virus Infection among Blood Donors in Jiangsu, China
Conclusions: This study showed that OCI does exist among Chinese blood donors. However, to determine the epidemiology and outcome of this HCV infection, further follow-up with more participants and patients receiving blood components with OCI is needed.Intervirology 2016;59:204-210 (Source: Intervirology)
Source: Intervirology - February 16, 2017 Category: Virology Source Type: research
An Outbreak of Acute Hepatitis Caused by Genotype IB Hepatitis A Viruses Contaminating the Water Supply in Thailand
Conclusions: Eleven of 24 fecal specimens collected from acute viral hepatitis patients were positive as determined by semi- nested reverse transcription PCR targeting the VP1/2A region of HAV. The nucleotide sequence of these samples had an identical genotype IB sequence, suggesting that the same causative agent was present. The complete nucleotide sequence derived from one of the samples indicated that the Thai genotype IB strain should be classified in a unique phylogenetic cluster. The analysis using an adjusted odds ratio showed that the consumption of groundwater was the most likely risk factor associated with the di...
Source: Intervirology - February 16, 2017 Category: Virology Source Type: research
Identification of the Nuclear Localization Signal Region of Duck Enteritis Virus UL14 and Its Interaction with VP16
Conclusions: Our findings indicated that pUL14 could transport VP16 into the nucleus and that the N-terminal 1-98 aa may contain the NLS domain of pUL14.Intervirology 2016;59:187-196 (Source: Intervirology)
Source: Intervirology - February 8, 2017 Category: Virology Source Type: research
Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients
Conclusion: Our findings indicate that B19 infection plays no role in leading chronic anemia in dialysis patients. However, persistent B19 viremia and the circulation of the same strains in dialysis patients may indicate a potential risk for the contamination of dialysis equipment and nosocomial spread of B19 infection within dialysis units.Intervirology 2016;59:179-185 (Source: Intervirology)
Source: Intervirology - January 31, 2017 Category: Virology Source Type: research
Genotype Distribution and Prevalence of Human Pegivirus among High-Risk Populations in Tunisia
Conclusion: This study enriches the limited data on HPgV prevalence in Tunisia, and shows, for the first time, the molecular epidemiology of the circulating strains in this country.Intervirology 2016;59:170-178 (Source: Intervirology)
Source: Intervirology - January 30, 2017 Category: Virology Source Type: research
Genotype Distribution and Prevalence of Human < b > < i > Pegivirus < /i > < /b > among High-Risk Populations in Tunisia
Conclusion: This study enriches the limited data on HPgV prevalence in Tunisia, and shows, for the first time, the molecular epidemiology of the circulating strains in this country.Intervirology 2016;59:170-178 (Source: Intervirology)
Source: Intervirology - January 27, 2017 Category: Virology Source Type: research
Coronavirus Infections in the Central Nervous System and Respiratory Tract Show Distinct Features in Hospitalized Children
Conclusion: To the best of our knowledge, this is the first report showing a high incidence of CoV infection in hospitalized children, especially with CNS illness. The characteristic cytokine expression profiles in CoV infection indicate the importance of host immune response in disease progression.Intervirology 2016;59:163-169 (Source: Intervirology)
Source: Intervirology - January 19, 2017 Category: Virology Source Type: research
Human Cytomegalovirus Infection in Nonimmunocompromised Patients: A Retrospective Analysis and Review of the Literature
We present 11 symptomatic cases of HCMV infection in nonimmunocompromised patients with non-life-threatening symptoms like long-lasting depletion, fatigue, and subfebrile fever with or without muscular pain and arthralgia. Although the symptoms were not life-threatening, all of the patients suffered a prolonged duration of the disease until improvement. These cases reinforce the important fact that HCMV infections in nonimmunocompromised patients are not always asymptomatic. Therefore, HCMV infection should always be included in the differential diagnosis of patients with unspecific signs of disease like depletion, subfebr...
Source: Intervirology - January 17, 2017 Category: Virology Source Type: research
Zika Virus-Induced Microcephaly and Its Possible Molecular Mechanism
Zika virus is an arthropod-borne re-emerging pathogen associated with the global pandemic of 2015-2016. The devastating effect of Zika viral infection is reflected by its neurological manifestations such as microcephaly in newborns. This scenario evoked our interest to uncover the neurotropic localization, multiplication of the virus, and the mechanism of microcephaly. The present report provides an overview of a possible molecular mechanism of Zika virus-induced microcephaly based on recent publications. Transplacental transmission of Zika viral infection from mother to foetus during the first trimester of pregnancy resul...
Source: Intervirology - January 12, 2017 Category: Virology Source Type: research
Viral Nucleic Acids in the Serum Are Dependent on Blood Sampling Site in Patients with Clinical Suspicion of Myocarditis
Conclusions: The detection of the same viral nucleic acid type in the myocardium and in ventricular serum being significantly more frequent than in the peripheral serum may suggest that the site of the blood collection is important for more precise and reliable confirmation of the active viral replication in the heart.Intervirology 2016;59:143-151 (Source: Intervirology)
Source: Intervirology - January 12, 2017 Category: Virology Source Type: research
Transforming Activity of Murine Herpesvirus 68 Putative Growth Factor Is Related to the Ability to Change Cytoskeletal Structure
Murine herpesvirus 68 (MHV-68) can transform cells in vitro and in vivo. We investigated putative murine herpesvirus growth factors (MHGFs) obtained by the separation of cell-free media from MHV-68-transformed cells on an FPLC Sephadex G15 column. The transforming activity of the MHGFA fraction was related to depolymerization of actin, disruption of the microtubule network, and punctate-reticular changes of the Golgi. The MHGFW fraction had only repressing activity on the transformed phenotype. Incubation of MRC-5 cells with MHGFW resulted in reticular changes of the Golgi apparatus, minor depolymerization of actin filamen...
Source: Intervirology - January 5, 2017 Category: Virology Source Type: research
HIV Drug Resistance and Phylogeny Profile in Na ïve and Antiretroviral-Experienced Patients in Tehran, Iran
Conclusion: The findings of this study illustrated the importance of resistance testing before and during ART treatment. This study can be used to set up a best medicine strategy in Iranian guidelines.Intervirology 2016;59:131-136 (Source: Intervirology)
Source: Intervirology - December 14, 2016 Category: Virology Source Type: research
Detection and Phylogenetic Analysis of Torque Teno Virus in Salivary and Tumor Biopsy Samples from Head and Neck Carcinoma Patients
Conclusions: Our data are compatible with the idea that TTV might act as a cocarcinogen in certain cases of HNCC. Alternatively, HNCC may facilitate either TTV replication or TTV entry into the saliva.Intervirology 2016;59:123-129 (Source: Intervirology)
Source: Intervirology - December 8, 2016 Category: Virology Source Type: research
Feasibility and Acceptability of Anal Self-Sampling for Human Papillomavirus Screening in HIV-Infected Patients
Conclusions: This study indicates that HPV detection and typing through anal self-sampling is a valuable strategy to screen patients at high risk for anal cancer development. This could allow earlier management of anal lesions and related cancer in patients at high risk for HPV.Intervirology 2016;59:118-122 (Source: Intervirology)
Source: Intervirology - December 6, 2016 Category: Virology Source Type: research
