Parthenolide Influences < b > < i > Herpes simplex < /i > < /b > v < b > < i > irus 1 < /i > < /b > Replication in vitro
Conclusion: Parthenolide exerts anti-HSV-1 activity by impairing cell viability, which consequently interferes with the efficient infection and production of new viral particles.Intervirology (Source: Intervirology)
Source: Intervirology - July 12, 2018 Category: Virology Source Type: research

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?
According to the latest update, 2,578 unique mature micro ­RNAs (miRNAs) are currently annotated in the human genome and participate in the regulation of multiple events, such as cellular proliferation or apoptosis. A previous study analyzing global miRNA expression patterns in GH cells (high human endogenous retrovirus, HERV, K vs. low) showed that 2 miR NAs (miR-663 and miR-638) are differentially regulated and exhibit expression parallel to that of HERV-K. The aim of this study was to evaluate HERV-K and -W pol gene and miR-155 expression in kidney transplant recipients and the possible relationship between them. Th...
Source: Intervirology - July 10, 2018 Category: Virology Source Type: research

Epidemiology and Genetic Diversity of Rotavirus in Kunming, China, in 2015
This study documents the molecular epidemiology, genetics, and diversity of rotavirus strains in children under 5 years of age in Kunming, China, and suggests that it may be important to offer RVA vaccination and scien tific evidence to stop and control RVA-related diarrhea.Intervirology (Source: Intervirology)
Source: Intervirology - July 5, 2018 Category: Virology Source Type: research

Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line
Conclusion: HSC 70 serves as a putative CHIKV receptor on C6/36 cells.Intervirology (Source: Intervirology)
Source: Intervirology - June 28, 2018 Category: Virology Source Type: research

Genetic Variability in E6 and E7 Oncogenes from Human Papillomavirus Type 58 in Mexican Women
Conclusion: The genetic variability of HPV58 from Mexico was described for the first time. E7 was more conserved than E6. New mutants present exclusively in Yucatan were identified.Intervirology (Source: Intervirology)
Source: Intervirology - June 26, 2018 Category: Virology Source Type: research

Changes in Hemagglutinin and Neuraminidase Genes of pH1N1 Influenza Virus Strains Collected from a North Indian Tertiary Care Hospital during 2015
Conclusions: Strains of pH1N1 isolated during year 2015 diverged from previously circulating strains. Their association with severity of illness needs to be further studied.Intervirology (Source: Intervirology)
Source: Intervirology - June 26, 2018 Category: Virology Source Type: research

Coxsackievirus A24 Variant Associated with Acute Haemorrhagic Conjunctivitis Cases, French Guiana, 2017
We report the full-length genomic sequences of 4 coxsackievirus A24 (CV-A24) isolates collected from AHC patients in French Guiana during this outbreak (May and June 2017). These isolates are very closely related and belong to the genotype IV of CV-A24 variant, which consists of strains sampled worldwide during AHC outbreaks in the 2000s and 2010s. No recombination events were detected within the genomic sequences, indicating that members of this genotype have continuously circulated worldwide for more than 10 years without undergoing recombination with other enteroviruses. This unusual trait could be due to their ocular t...
Source: Intervirology - June 13, 2018 Category: Virology Source Type: research

Launching a Global Network of Virologists: The World Society for Virology (WSV)
Intervirology (Source: Intervirology)
Source: Intervirology - June 6, 2018 Category: Virology Source Type: research

< b > < i > Zika virus < /i > < /b > Pathogenesis in Infant Mice after Natural Transmission by the Bite of Infected Mosquitoes
Conclusions: This study demonstrates ZIKV pathogenesis in infant CD1 mice and that these mice are highly susceptible to natural infection with this ZIKV strain.Intervirology (Source: Intervirology)
Source: Intervirology - March 29, 2018 Category: Virology Source Type: research

Membrane-Associated Kaposi Sarcoma-Associated Herpesvirus Glycoprotein B Promotes Cell Adhesion and Inhibits Migration of Cells via Upregulating IL-1 β and TNF-α
Conclusion: Our results provide novel evidence that demonstrates full-length gB expressed on cell membrane to mediate adhesion and inhibit migration of cells not only by autocrine mechanism mediated by RGD-based interactions [Hussein et al.: BMC Cancer 2016; 16: 148], but also by paracrine mechanism mediated by gB-induced IL-1 β and TNF-α.Intervirology (Source: Intervirology)
Source: Intervirology - March 29, 2018 Category: Virology Source Type: research

Development of an Immunoassay for Detection of Torque Teno Virus (TTV) Antibodies Using the N22 Expression Product from TTV Genotype 2
Conclusion: The present immunoassay using the N22 expression product may be used as an alternative to PCR to detect TTV infection in large populations.Intervirology 2017;60:207 –216 (Source: Intervirology)
Source: Intervirology - March 27, 2018 Category: Virology Source Type: research

Genetic Variability and Sequence Relatedness of Matrix Protein in Viruses of the Families Paramyxoviridae and Pneumoviridae
Conclusion: While we have confirmed that M proteins were conserved among individual viruses, any changes that are observed should be given attention and further researched. Of special interest is inclusion of HPIV2 M proteins in this analysis, as these proteins have not been studied to the same extent as other paramyxoviruses.Intervirology (Source: Intervirology)
Source: Intervirology - March 6, 2018 Category: Virology Source Type: research

Establishment of a Competitive Binding Assay Identifying the Different Characteristics of Neutralizing Epitopes of Hepatitis E Virus
Conclusion: The results of competitive binding assays revealed that the binding of HRP-conjugated 2B1 to p166W01 could not be inhibited by 5G5 or 3G1. Similarly, the binding of HRP-conjugated 4C5 to p166Chn could not be inhibited by 5G5 or 3G1. However, the mAbs 5G5 and 3G1 blocked each other ’s binding to p166W01 and p166Chn, suggesting that common and genotype-specific neutralizing mAbs recognize independent epitopes.Intervirology (Source: Intervirology)
Source: Intervirology - March 6, 2018 Category: Virology Source Type: research

Aichivirus in Children with Diarrhea in Northern Italy
Conclusions: Our results indicate that AiV may be associated with a limited number of diarrhea cases in pediatric patients.Intervirology (Source: Intervirology)
Source: Intervirology - March 2, 2018 Category: Virology Source Type: research

Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression
Conclusion: E1 may play a role in cancer development. The detection of E1 mRNA and promoter methylation may be useful as cancer prognostic markers.Intervirology (Source: Intervirology)
Source: Intervirology - March 1, 2018 Category: Virology Source Type: research

Detection of Genotype 1a and 1f of Hepatitis E Virus in Patients Treated at Tertiary Care Hospitals in Central India
Conclusion: HEV is a major aetiological agent of viral hepatitis in central India with adults and males at higher risk of infection. Two subtypes of the virus were detected in the region. Continuous serological surveillance and molecular monitoring will help to understand the epidemiology of HEV infection, outbreak mitigation and aid in providing treatment.Intervirology (Source: Intervirology)
Source: Intervirology - March 1, 2018 Category: Virology Source Type: research

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq
Conclusions: This study confirmed the presence of BPV-1, BPV-2, and BPV-13, which belong to theDeltapapillomavirus genera, for the first time in the DNA of Iraqi cattle. Understanding BPV diversity and epidemiology is of critical importance for starting prevention strategies.Intervirology (Source: Intervirology)
Source: Intervirology - February 9, 2018 Category: Virology Source Type: research

Cytomegalovirus Glycoprotein B Genotype Distribution in Italian Transplant Patients
Conclusions: Mixed infections seem to be prevalent in Italian transplant patients; it is likely that mixed infections are more difficult to control by immunological response in comparison to single genotype infections. In this context, the genetic profile of infecting viruses and relation to clinical outcome should be investigated, also taking into account the CMV-specific cellular immune response.Intervirology (Source: Intervirology)
Source: Intervirology - February 2, 2018 Category: Virology Source Type: research

Type-Specific Human Papillomavirus Prevalence in Iranian Women with Normal Cervical Cytology: The Impact of Current HPV Vaccines
Objective: Human papillomavirus (HPV) vaccination is currently not included in the national vaccination program in Iran. Regional data on the distribution of HPV types in women are important as they can predict the impact of currently available vaccines and help health policy makers to consider all the possibilities with regard to HPV vaccination.Methods: A total of 1,218 Iranian women with normal cervical cytology were included in this study. The presence of the HPV genome was investigated in all specimens by PCR assay, and all HPV-positive samples were genotyped.Results: Totally, HPV was detected in 88 samples (7.2%). Ac...
Source: Intervirology - January 24, 2018 Category: Virology Source Type: research

Survival of Viruses in Water
Water, a frequent vehicle for the transmission of viruses, may permit their survival, but many environmental factors will have an adverse effect on the viral population. Risk evaluation requires identification of these factors and assessment of the inactivation rate of infectious viruses. A higher temperature means a faster reduction of the viral population, as do increased sunlight, higher antimicrobial concentration, or higher oxygen levels. Another documented impact is linked to the presence of indigenous microbial populations: virus survival is higher in sterile water. Environmental factors inactivate viruses through d...
Source: Intervirology - January 9, 2018 Category: Virology Source Type: research

Preliminary Characterization of El Tor Vibriophage M4
Conclusions: M4 belongs to the Myoviridae family and it is generally thermostable. It is prone to destruction by alkali and UV. It also has a large DNA which is densely packed inside of a big capsid.Intervirology (Source: Intervirology)
Source: Intervirology - January 5, 2018 Category: Virology Source Type: research

Resistance-Associated Mutations and Polymorphisms among Integrase Inhibitor-Na ïve HIV-1 Patients in Kuwait
Conclusion: Our results highlight the importance of monitoring the emergence of INSTI-RAMS before and after the initiation of INSTI-based therapy.Intervirology (Source: Intervirology)
Source: Intervirology - December 6, 2017 Category: Virology Source Type: research

CRF35-AD as the Main Circulating Genotype of Human Immunodeficiency Virus Type 1 Infection in Iran: A Phylogenetic and Demographic-Based Study
Finding the predominant circulating subtype of human immunodeficiency virus type 1 (HIV-1) and surveying co-infection with other infectious viruses are crucial to making preventive decisions. To this end, 50 Iranian HIV-positive patients made up of 37 men and 13 women were selected. Most of the HIV-positive patients (70%) were intravenous drug users (IDUs), and 48 and 32% of patients were co-infected with HCV and HBV, respectively. The rate of simultaneous infection with HIV, HCV, and HBV was found to be 6%. The p17 region of thegag and the c2-v5 region of theenv genes were sequenced and then clustered by phylogenetic anal...
Source: Intervirology - November 29, 2017 Category: Virology Source Type: research

Distribution of Hepatitis C Virus Genotypes and Subtypes in the Metropolitan Area of Naples, Italy, in the Era of Interferon-Free Regimens
The objective of this work was to study the distribution of hepatitis C virus (HCV) genotypes and subtypes from 2010 to 2015 in 1,221 anti-HCV/HCV-RNA-positive specimens from patients living in the metropolitan area of Naples, since HCV genotypes and subtypes remain cornerstones in the management of chronic HCV infection even in the directly acting antivirals era.Methods: The study was carried out on 1,221 anti-HCV/HCV-RNA-positive plasma samples collected between April 2010 and December 2015.Results: Of the 1,221 patients enrolled, 633 (51.9%) were males and 588 (48.1%) were females, with a mean age of 60 ± 13 (SD)...
Source: Intervirology - November 21, 2017 Category: Virology Source Type: research

Intergenotype Recombination among New Norovirus GII.4 Variants in the Complete Genome
In this study, we sought to examine the role that intergenotype recombination has played in the emergence of GII.4 Sydney 2012 variants. The results show that the GII.4 Sydney 2012 variants, Kawasaki194 and CA3477, were intergenotype recombination NoV strains with a GII.4 capsid and a GII.P16 polymerase gene. It has been reported for the first time that GII.4 new variant recombinants come from intergenotype recombination of GII.P16 and GII.4 strains in the complete genome.Intervirology (Source: Intervirology)
Source: Intervirology - November 21, 2017 Category: Virology Source Type: research

Comparing the Clinical Features and Outcomes of Acute Hepatitis E Viral Infections with Those of Acute Hepatitis A, B, and C Infections in Korea
Conclusion: Our findings indicate that HEV infection in Korea is frequently transmitted through the consumption of raw meat and may cause acute or chronic liver disease.Intervirology (Source: Intervirology)
Source: Intervirology - November 16, 2017 Category: Virology Source Type: research

Evaluation of Temporal Virological Responses to Interferon- α-2b plus Ribavirin among Genotype 3a Hepatitis C Virus-Infected Patients
Conclusions: Individuals incompliant with their treatment who have a higher RVR significantly influence their SVR towards a better remission that can be treated within a short duration with standard treatment.Intervirology (Source: Intervirology)
Source: Intervirology - November 15, 2017 Category: Virology Source Type: research

No Correlation of the Disease Severity of Influenza A Virus Infection with the rs12252 Polymorphism of the Interferon-Induced Transmembrane Protein 3 Gene
The 2009 H1N1 influenza pandemic, which involved a more pathogenic virus than seasonal influenza viruses, rapidly spread around the world and caused many deaths in humans. The members of the interferon-induced transmembrane (IFITM) protein family prevent viral replication and are crucial for defending the host cell against influenza A virus (IAV). Several studies suggest that the CC genotype at the single nucleotide polymorphism (SNP) rs12252 ofIFITM3 confers a genetic predisposition to pandemic influenza A in Europeans and Han Chinese, although one study in a British cohort failed to show an association. In order to exami...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection
Infection of human MRC-5 cells and mouse NIH-3T3 cells with a murine gamma-herpesvirus (MuHV-4 strain 68; MHV-68) photoinactivated by visible light in the presence of methylene blue (MB) resulted in nonproductive infection and the appearance of morphologically transformed cells. Two stably transformed cell lines were derived from both of these cell types and were confirmed to contain both viral DNA and antigen. Next, a quiescent MHV-68 infection in MRC-5 and NIH-3T3 cells was established after cultivation at 41 °C in the presence of phosphonoacetic acid. Following the exposure of quiescently infected cells to visible l...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Detection of Merkel Cell Polyomavirus in Respiratory Tract Specimens
Conclusions: MCPyV was observed in specimens from patients with acute respiratory diseases, indicating that there may be a relationship between the virus and these diseases. We were not able to detect MCPyV in samples from patients with chronic lung diseases, including lung cancer, suggesting no association with MCPyV infection and no involvement of this polyomavirus in lung cancerogenesis.Intervirology 2017;60:28-32 (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A
Conclusion: There is a direct correlation of quantities of syncytia and inoculum size, but not with mRNA levels of HRSV-A F protein. Importantly, levels of F protein mRNA were directly related to progeny production.Intervirology 2017;60:56-60 (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Identifying Attenuating Mutations: Tools for a New Vaccine Design against < b > < i > Flaviviruses < /i > < /b >
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

The Histopathological Characteristics Caused by < b > < i > Trionyx sinensis < /i > < /b > Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles ( < b > < i > Pelodiscus sinensis < /i > < /b > )
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Concurrent Infection with < b > < i > Plasmodium vivax < /i > < /b > and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR
Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens
Conclusions: The formulation tests showed that a combination of 0.02 μg of HBs, 0.2 μg of preS1-preS2-HBs, and 0.02 μg of HBc was effective in eliciting specific antibodies in mice.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Phylogenetic Analysis of Torque Teno Virus in Thalassemic Children in Egypt
Conclusion: TTV is highly prevalent among children with thalassemia in Egypt, with a relatively high infection rate also detected among healthy children.Intervirology (Source: Intervirology)
Source: Intervirology - October 25, 2017 Category: Virology Source Type: research

Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens
Conclusions: The formulation tests showed that a combination of 0.02 μg of HBs, 0.2 μg of preS1-preS2-HBs, and 0.02 μg of HBc was effective in eliciting specific antibodies in mice.Intervirology (Source: Intervirology)
Source: Intervirology - September 28, 2017 Category: Virology Source Type: research

Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR
Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.Intervirology (Source: Intervirology)
Source: Intervirology - September 27, 2017 Category: Virology Source Type: research

Concurrent Infection with Plasmodium vivax and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - September 15, 2017 Category: Virology Source Type: research

Concurrent Infection with < b > < i > Plasmodium vivax < /i > < /b > and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - September 14, 2017 Category: Virology Source Type: research

The Histopathological Characteristics Caused by Trionyx sinensis Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles (Pelodiscus sinensis)
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - September 7, 2017 Category: Virology Source Type: research

The Histopathological Characteristics Caused by < b > < i > Trionyx sinensis < /i > < /b > Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles ( < b > < i > Pelodiscus sinensis < /i > < /b > )
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - September 6, 2017 Category: Virology Source Type: research

Identifying Attenuating Mutations: Tools for a New Vaccine Design against Flaviviruses
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - September 5, 2017 Category: Virology Source Type: research

Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A
Conclusion: There is a direct correlation of quantities of syncytia and inoculum size, but not with mRNA levels of HRSV-A F protein. Importantly, levels of F protein mRNA were directly related to progeny production.Intervirology (Source: Intervirology)
Source: Intervirology - September 4, 2017 Category: Virology Source Type: research

Identifying Attenuating Mutations: Tools for a New Vaccine Design against < b > < i > Flaviviruses < /i > < /b >
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - September 4, 2017 Category: Virology Source Type: research

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection
Infection of human MRC-5 cells and mouse NIH-3T3 cells with a murine gamma-herpesvirus (MuHV-4 strain 68; MHV-68) photoinactivated by visible light in the presence of methylene blue (MB) resulted in nonproductive infection and the appearance of morphologically transformed cells. Two stably transformed cell lines were derived from both of these cell types and were confirmed to contain both viral DNA and antigen. Next, a quiescent MHV-68 infection in MRC-5 and NIH-3T3 cells was established after cultivation at 41 °C in the presence of phosphonoacetic acid. Following the exposure of quiescently infected cells to visible l...
Source: Intervirology - August 24, 2017 Category: Virology Source Type: research

Detection of Merkel Cell Polyomavirus in Respiratory Tract Specimens
Conclusions: MCPyV was observed in specimens from patients with acute respiratory diseases, indicating that there may be a relationship between the virus and these diseases. We were not able to detect MCPyV in samples from patients with chronic lung diseases, including lung cancer, suggesting no association with MCPyV infection and no involvement of this polyomavirus in lung cancerogenesis.Intervirology (Source: Intervirology)
Source: Intervirology - August 24, 2017 Category: Virology Source Type: research

No Correlation of the Disease Severity of Influenza A Virus Infection with the rs12252 Polymorphism of the Interferon-Induced Transmembrane Protein 3 Gene
The 2009 H1N1 influenza pandemic, which involved a more pathogenic virus than seasonal influenza viruses, rapidly spread around the world and caused many deaths in humans. The members of the interferon-induced transmembrane (IFITM) protein family prevent viral replication and are crucial for defending the host cell against influenza A virus (IAV). Several studies suggest that the CC genotype at the single nucleotide polymorphism (SNP) rs12252 ofIFITM3 confers a genetic predisposition to pandemic influenza A in Europeans and Han Chinese, although one study in a British cohort failed to show an association. In order to exami...
Source: Intervirology - August 16, 2017 Category: Virology Source Type: research