Concurrent Infection with < b > < i > Plasmodium vivax < /i > < /b > and the Dengue and Chikungunya Viruses in a Paediatric Patient from New Delhi, India in 2016
We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis. Mixed infection with multiple pathogens is a challenge for accurate diagnosis due to the overlapping clinical symptoms. The accurate and timely diagnosis of multiple pathogens in such cases is important for rapid and effective patient manageme...
Source: Intervirology - September 14, 2017 Category: Virology Source Type: research
The Histopathological Characteristics Caused by Trionyx sinensis Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles (Pelodiscus sinensis)
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - September 7, 2017 Category: Virology Source Type: research
The Histopathological Characteristics Caused by < b > < i > Trionyx sinensis < /i > < /b > Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles ( < b > < i > Pelodiscus sinensis < /i > < /b > )
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues ofPelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infectedP. sinensiswere analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues ofP. sinensis.The t...
Source: Intervirology - September 6, 2017 Category: Virology Source Type: research
Identifying Attenuating Mutations: Tools for a New Vaccine Design against Flaviviruses
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - September 5, 2017 Category: Virology Source Type: research
Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A
Conclusion: There is a direct correlation of quantities of syncytia and inoculum size, but not with mRNA levels of HRSV-A F protein. Importantly, levels of F protein mRNA were directly related to progeny production.Intervirology (Source: Intervirology)
Source: Intervirology - September 4, 2017 Category: Virology Source Type: research
Identifying Attenuating Mutations: Tools for a New Vaccine Design against < b > < i > Flaviviruses < /i > < /b >
EmergingFlaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against otherFlaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuatedFlavivirusvaccines using molecular biology methods is now possible. Molecular infectious clones of the ...
Source: Intervirology - September 4, 2017 Category: Virology Source Type: research
Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection
Infection of human MRC-5 cells and mouse NIH-3T3 cells with a murine gamma-herpesvirus (MuHV-4 strain 68; MHV-68) photoinactivated by visible light in the presence of methylene blue (MB) resulted in nonproductive infection and the appearance of morphologically transformed cells. Two stably transformed cell lines were derived from both of these cell types and were confirmed to contain both viral DNA and antigen. Next, a quiescent MHV-68 infection in MRC-5 and NIH-3T3 cells was established after cultivation at 41 °C in the presence of phosphonoacetic acid. Following the exposure of quiescently infected cells to visible ligh...
Source: Intervirology - August 24, 2017 Category: Virology Source Type: research
Detection of Merkel Cell Polyomavirus in Respiratory Tract Specimens
Conclusions: MCPyV was observed in specimens from patients with acute respiratory diseases, indicating that there may be a relationship between the virus and these diseases. We were not able to detect MCPyV in samples from patients with chronic lung diseases, including lung cancer, suggesting no association with MCPyV infection and no involvement of this polyomavirus in lung cancerogenesis.Intervirology (Source: Intervirology)
Source: Intervirology - August 24, 2017 Category: Virology Source Type: research
No Correlation of the Disease Severity of Influenza A Virus Infection with the rs12252 Polymorphism of the Interferon-Induced Transmembrane Protein 3 Gene
The 2009 H1N1 influenza pandemic, which involved a more pathogenic virus than seasonal influenza viruses, rapidly spread around the world and caused many deaths in humans. The members of the interferon-induced transmembrane (IFITM) protein family prevent viral replication and are crucial for defending the host cell against influenza A virus (IAV). Several studies suggest that the CC genotype at the single nucleotide polymorphism (SNP) rs12252 ofIFITM3 confers a genetic predisposition to pandemic influenza A in Europeans and Han Chinese, although one study in a British cohort failed to show an association. In order to exami...
Source: Intervirology - August 16, 2017 Category: Virology Source Type: research
The First Characterization of HIV-1 Subtypes and Drug Resistance Mutations among Antiretrovirally Treated Patients in Kermanshah, Iran
Conclusions: Our results showed a high prevalence of drug resistance mutations in strains isolated from patients on treatment. At our center, we therefore recommend that genotyping be performed. This would allow the physician to prescribe appropriate drugs, reduce treatment costs, and increase the longevity and quality of life of patients.Intervirology (Source: Intervirology)
Source: Intervirology - August 9, 2017 Category: Virology Source Type: research
Evolution and Emergence of Pathogenic Viruses: Past, Present, and Future
Incidences of emerging/re-emerging deadly viral infections have significantly affected human health despite extraordinary progress in the area of biomedical knowledge. The best examples are the recurring outbreaks of dengue and chikungunya fever in tropical and sub-tropical regions, the recent epidemic of Zika in the Americas and the Caribbean, and the SARS, MERS, and influenza A outbreaks across the globe. The established natural reservoirs of human viruses are mainly farm animals, and, to a lesser extent, wild animals and arthropods. The intricate “host-pathogen-environment” relationship remains the key to understand...
Source: Intervirology - August 3, 2017 Category: Virology Source Type: research
High Frequency of Human Leukocyte Antigen-B*57:01 Allele Carriers among HIV-Infected Patients in Serbia
This study was designed to estimate the prevalence of the HLA-B*57:01 allele in Serbian HIV-1-infected patients. The presence of the HLA-B*57:01 allele was analyzed in 273 HIV-1-infected patients aged 18 years or more, who were abacavir na ïve. Buccal swab samples were obtained from all participants and assayed for the presence of HLA-B*57:01 using a commercially available HLA-B*57:01 real-time PCR kit. The presence of the HLA-B*57:01 allele was found in 22 of 273 tested individuals (8%; 95% CI 5.4-11.9%). This is the first study tha t estimated the HLA-B*57:01 prevalence among HIV-infected patients in Serbia. The very hi...
Source: Intervirology - August 3, 2017 Category: Virology Source Type: research
Contents Vol. 59, 2016
Intervirology 2016;59:I-IV (Source: Intervirology)
Source: Intervirology - July 20, 2017 Category: Virology Source Type: research
Distribution of Human Papillomavirus Genotypes among Women in Mashhad, Iran
Conclusions: Prevention, early diagnosis, and early treatment have been proven to reduce the mortality rate of cervical cancer. Therefore, an accurate diagnosis of the genotype of the virus in infected patients is very important.Intervirology (Source: Intervirology)
Source: Intervirology - July 19, 2017 Category: Virology Source Type: research
Middle East Respiratory Syndrome Coronavirus and Pulmonary Tuberculosis Coinfection: Implications for Infection Control
Coinfection of Middle East respiratory syndrome coronavirus (MERS-CoV) with tuberculosis (TB) has not been previously reported. Here, we present 2 cases with both MERS-CoV and pulmonary TB. The first case was a 13-year-old patient who was admitted with a 2-month history of fever, weight loss, night sweats, and cough. The second patient was a 30-year-old female who had a 4-week history of cough associated with shortness of breath and weight loss of 2 kg. The 2 patients were diagnosed with pulmonary TB and had positive MERS-CoV. Both patients were discharged to complete their therapy for TB at home. It is likely that both pa...
Source: Intervirology - July 5, 2017 Category: Virology Source Type: research
