Placenta-specific lncRNA 1600012P17Rik is expressed in spongiotrophoblast and glycogen trophoblast cells of mouse placenta
In this study, we explored mouse placenta-associated lncRNAs using a combined bioinformatic and experimental approach. We used the FANTOM5 database to survey lncRNA expression in mouse placenta and found that1600012P17Rik (MGI: 1919275, designated P17Rik), a long intergenic ncRNA, was the most highly expressed lncRNA at gestational day 17. Polymerase chain reaction analysis confirmed that P17Rik was exclusively expressed in placenta and not in any of the adult organs examined in this study. In situ hybridization analysis revealed that it was highly expressed in spongiotrophoblast cells and to a lesser extent in glycogen ...
Source: Histochemistry and Cell Biology - April 29, 2022 Category: Biomedical Science Source Type: research
Correction to: Quantitative evaluation of spermatogenesis by fluorescent histochemistry
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 27, 2022 Category: Biomedical Science Source Type: research
Paneth cell maturation is related to epigenetic modification during neonatal –weaning transition
In this study, we investigated the histological changes including epigenetic modification in the mouse ileum postnatally and assessed the effect of the methyltransferase inhibitor on epithelium development using an organoid culture. The morphological and functional maturation of Paneth cells occurred in the first 2 weeks and was accompanied by histone H3 lysine 27 (H3K27) trimethylation, although signifi cant differences in DNA methylation or other histone H3 trimethylation were not observed. Inhibition of H3K27 trimethylation in mouse ileal organoids suppressed crypt formation and Paneth cell maturation, until around P10...
Source: Histochemistry and Cell Biology - April 25, 2022 Category: Biomedical Science Source Type: research
Neuronal internalization of immunoglobulin G injected into the mouse brain by a novel absorption strategy to avoid unwanted interaction with immune complex using centrifugal filtration
This study is the first demonstration of neuronal redistribution of injected IgG in the mouse brain. Neuronal internaliza tion of exogenous IgG may be advantageous especially when the therapeutic targets of monoclonal IgG are intraneuronal such as neurofibrillary tangles or Lewy bodies. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 25, 2022 Category: Biomedical Science Source Type: research
Retinoblastoma patient-derived stem cells —an in vivo model to study the role of RB1 in adipogenesis
AbstractRetinoblastoma (RB1) protein is a multifunctional protein that plays an important role in cell cycle regulation and cell differentiation, including adipogenesis. A detailed literature search to understand the role of RB1 in adipogenesis revealed that the nature of the RB1 inactivation (in vivo/in vitro) led to differences in adipogenesis. The majority of these studies were animal-based, and the only study in humans employed an in vitro mode of RB1 inactivation. To overcome these differences and lack of human studies, we sought to explore the role of RB1 in adipogenesis using orbi tal adipose mesenchymal stem ce...
Source: Histochemistry and Cell Biology - April 21, 2022 Category: Biomedical Science Source Type: research
Expression of the histone lysine methyltransferases SETD1B, SETDB1, SETD2, and CFP1 exhibits significant changes in the oocytes and granulosa cells of aged mouse ovaries
In conclusion, these findings suggest that altered expression of the histone methyltransferase genes in the ovarian cells may be associated with female fertility loss in advancing maternal age. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 20, 2022 Category: Biomedical Science Source Type: research
Nestin and osteocrin mRNA increases in human semitendinosus myotendinous junction 7 days after a single bout of eccentric exercise
In this study, 30 subjects were randomized to a single bout of eccentric exercise 1 week prior to tissue sampling (exercised) or no exercise (control). Samples were collected from the semitendinosus as part of reconstruction of the anterior cruciate ligament and divided into fractions containing muscle, MTJ and tendon, respectively. The concentrations of macrophages and satellite cells were counted, and the expression of genes previously known to be active at the MTJ were analyzed by real-time–quantitative PCR. An effect of the single bout of exercise was found on the expression of nestin (NES) and osteocrin (OSTN) mRN...
Source: Histochemistry and Cell Biology - April 15, 2022 Category: Biomedical Science Source Type: research
A simple preparation step to remove excess liquid lipids in white adipose tissue enabling improved detection of metabolites via MALDI-FTICR imaging MS
In this study, we integrated a simple and low-cost preparation step into the existing MALDI-FTICR imaging MS pipeline. The new method —referred to as filter paper application—is characterized by an easy sample handling and high reproducibility. The aforementioned filter paper is placed onto the tissue prior to matrix application in order to remove the layer of excess liquid lipids. Consequently, MALDI-FTICR imaging MS detectio n was significantly improved, resulting in a higher number of detectedm/z species and higher ion intensities. After analyzing various durations of filter paper application, 30 s was found to be...
Source: Histochemistry and Cell Biology - April 7, 2022 Category: Biomedical Science Source Type: research
Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination
In conclusion, our results acknowledged that GFAP, vimentin, LCN2, and ALDH1L1 serve as reliable marker to identify activated astrocytes during cuprizone-induced de- and remyelination. Moreover, there were clear regional and temporal differences in protein and mRNA expression levels and patterns of the studied markers, generally between gray and white matter structures. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 5, 2022 Category: Biomedical Science Source Type: research
A novel method for histological examination of hair follicles
AbstractIntact and healthy hair follicles are important for hair growth after hair follicle transplantation. However, effective and practical evaluation methods for the quality of hair follicles are currently lacking. In the present study, we developed a novel fast staining method for histological examination of hair follicles. The whisker follicles from mice were used to explore the staining protocols, and the final protocol for the evaluation of human hair follicles was derived from animal experiments. After extraction, human hair follicles or mouse whisker follicles were permeabilized with 0.3% Triton X-100. Subsequentl...
Source: Histochemistry and Cell Biology - April 4, 2022 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 4, 2022 Category: Biomedical Science Source Type: research
MUC6 expression is a preferable prognostic marker for invasive mucinous adenocarcinoma of the lung
AbstractGastric gland mucin consists of core protein MUC6 with residues heavily glycosylated by uniqueO-glycans carrying α1,4-linkedN-acetylglucosamine ( αGlcNAc). αGlcNAc-glycosylated MUC6 protein is seen in normal gastric and duodenal glands. Decreased αGlcNAc glycosylation on MUC6-positive tumor cells is often observed in premalignant lesions of the stomach, pancreas, and bile duct, and decreased MUC6 expression is seen in invasive cancer of th ese organs. Lung cancer (LC) is the most common cause of cancer death worldwide. Recently, the adenocarcinoma subtype has become the most common histological subtype of LC, a...
Source: Histochemistry and Cell Biology - March 30, 2022 Category: Biomedical Science Source Type: research
Early unhealthy eating habits underlie morpho-functional changes in the liver and adipose tissue in male rats
AbstractEarly-life consumption of high-fat and sugar-rich foods is recognized as a major contributor for the onset of metabolic dysfunction and its related disorders, including diabetes and nonalcoholic fatty liver disease. The lifelong impact of early unhealthy eating habits that start at younger ages remains unclear. Therefore, to better understand the effects of diet, it is essential to evaluate the structural and functional changes induced in metabolic organs and potential mechanisms underlying those changes. To investigate the long-term effects of eating habits, young male rats were exposed to high-sugar and high-ener...
Source: Histochemistry and Cell Biology - March 28, 2022 Category: Biomedical Science Source Type: research
A joint alignment and reconstruction algorithm for electron tomography to visualize in-depth cell-to-cell interactions
AbstractElectron tomography allows one to obtain 3D reconstructions visualizing a tissue ’s ultrastructure from a series of 2D projection images. An inherent problem with this imaging technique is that its projection images contain unwanted shifts, which must be corrected for to achieve reliable reconstructions. Commonly, the projection images are aligned with each other by means of f iducial markers prior to the reconstruction procedure. In this work, we propose a joint alignment and reconstruction algorithm that iteratively solves for both the unknown reconstruction and the unintentional shift and does not require any ...
Source: Histochemistry and Cell Biology - March 23, 2022 Category: Biomedical Science Source Type: research
Fluorescence lifetime imaging and electron microscopy: a correlative approach
This study delineates the correlative imaging of cells with FLIM and electron microscopy (EM). Human fibroblasts were cultivated in a microscopy slide bearing a coordinate system and FLIM measurement was conducted. Following chemical fixation, embedding in Epon and cutting with an ultramicrotome, tomograms of selected cells were acquired with a scanning transmission electron microscope (STEM). Correlative imaging of antimycin A-treated fibroblasts shows a decrease in fluorescence lifetime as well as swollen mitochondria with large cavities in STEM tomography. To our knowledge, this is the first correlative FLIM and EM wo...
Source: Histochemistry and Cell Biology - March 10, 2022 Category: Biomedical Science Source Type: research
