MicroRNA-598 inhibition ameliorates LPS-induced acute lung injury in mice through upregulating Ebf1 expression
In this study, we found that the expression of miR-598 was significantly upregulated in the lung tissues of mice with lipopolysaccharide (LPS)-induced acute lung injury. Both loss-of-function and gain-of-function studies were performed to evaluate the function of miR-598 in acute lung injury. The results showed that inhibition of miR-598 attenuated inflammatory response, oxidative stress, and lung injury in mice treated with LPS, while overexpression of miR-598 exacerbated the LPS-induced acute lung injury. Mechanistically, transcription factor Early B-cell Factor-1 (Ebf1) was predicted and validated as a downstream target...
Source: Histochemistry and Cell Biology - April 28, 2023 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 18, 2023 Category: Biomedical Science Source Type: research
Big data in contemporary electron microscopy: challenges and opportunities in data transfer, compute and management
AbstractThe second decade of the twenty-first century witnessed a new challenge in the handling of microscopy data. Big data, data deluge, large data, data compliance, data analytics, data integrity, data interoperability, data retention and data lifecycle are terms that have introduced themselves to the electron microscopy sciences. This is largely attributed to the booming development of new microscopy hardware tools. As a result, large digital image files with an average size of one terabyte within one single acquisition session is not uncommon nowadays, especially in the field of cryogenic electron microscopy. This bri...
Source: Histochemistry and Cell Biology - April 13, 2023 Category: Biomedical Science Source Type: research
Clay microparticles for the enhancement of bone regeneration: in vitro studies
In this study , we evaluated the potential of palygorskite clay mineral microparticles for the promotion of the osteogenic differentiation in human mesenchymal stem cells (hMSCs) in vitro. Alkaline phosphatase (ALP) activity and Alizarin red staining showed that osteogenic differentiation of hMSCs is enhanced in the presence of palygorskite clay. Although, gene expression analysis did not reveal upregulation of several osteogenic markers in the presence of the clay microparticles, another interaction mechanism resulted in the enhanced osteogenic differentiation of hMSCs. The charged surfaces of the palygorskite clay pa r...
Source: Histochemistry and Cell Biology - April 8, 2023 Category: Biomedical Science Source Type: research
Identification and protective role of CD34+ stromal cells/telocytes in experimental autoimmune encephalomyelitis (EAE) mouse spleen
AbstractExperimental autoimmune encephalomyelitis (EAE) is a classical animal model of human multiple sclerosis (MS) that is most commonly used to study the neuropathology and therapeutic effects of the disease. Telocytes (TCs) are a specialized type of interstitial or mesenchymal cell first identified by Popescu in various tissues and organs. However, the existence, distribution and role of CD34+ stromal cells (SCs)/TCs in the EAE-induced mouse spleen remain to be elucidated. We conducted immunohistochemistry, immunofluorescence (double staining for CD34 and c-kit, vimentin, F4/80, CD163, Nanog, Sca-1, CD31 or tryptase)...
Source: Histochemistry and Cell Biology - April 4, 2023 Category: Biomedical Science Source Type: research
Impact of antigen retrieval protocols on the immunohistochemical detection of epigenetic DNA modifications
This study compares three different pretreatment protocols for the immunohistochemical detection of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in nuclear DNA. The human biological samples analyzed included formalin-fixed and paraffin-embedded (FFPE) normal squamous epithelium, ethanol-fixed cultured cells, and metaphase chromosomes. The antigen retrieval methods included low pH Citrate and high pH Tris –ethylenediaminetetraacetic acid (EDTA) protocols, as well as a method using Pepsin pretreatment combined with HCl for DNA denaturation. A gradual increase in the detection levels of 5-mC and 5-hmC was obs...
Source: Histochemistry and Cell Biology - April 3, 2023 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 28, 2023 Category: Biomedical Science Source Type: research
An investigation of the potential effects of amitriptyline on polycystic ovary syndrome induced by estradiol valerate
AbstractPolycystic ovarian syndrome (PCOS) is frequently observed in adolescent women and usually progresses with depression. The aim of this study was to examine the effects of amitriptyline (Ami), a drug used in the treatment of depression, in individuals with PCOS. Forty 12-week-old female Wistar albino rats were randomly divided into five groups: control, sham, PCOS, Ami, and PCOS + Ami. To induce the syndrome in the PCOS groups, a single dose of 4 mg/kg estradiol valerate was administered by intraperitoneal injection; 10 mg/kg Ami was administered by intraperitoneal injection for 30 days in the Ami groups. Aft...
Source: Histochemistry and Cell Biology - March 27, 2023 Category: Biomedical Science Source Type: research
Histological study of the role of CD34+ stem cells and mast cells in cyclophosphamide-induced thymic injury in rats and the possible attenuating role of melatonin
In conclusion, melatonin may protect against CP-induced thymic injury. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 8, 2023 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 6, 2023 Category: Biomedical Science Source Type: research
Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFR
AbstractEndocytosis, an important macromolecule uptake process in cells, is known to be dysregulated in cancer. Clathrin and caveolin-1 proteins play a major role in receptor-mediated endocytosis. We have used a quantitative, unbiased and semi-automated method to measure in situ protein expression of clathrin and caveolin-1 in cancerous and paired normal (cancer adjacent, non-cancerous) human prostate tissue. There was a significant (p < 0.0001) increase in the expression of clathrin in prostate cancer samples (N = 29,n = 91) compared to normal tissue (N = 29,n = 67) (N = number of patients,n ...
Source: Histochemistry and Cell Biology - March 4, 2023 Category: Biomedical Science Source Type: research
nanoGold and µGold inhibit autoimmune inflammation: a review
AbstractThe newest data on metallic gold have placed the noble metal central in the fight for the safe treatment of autoimmune inflammation. There are two different ways to use gold for the treatment of inflammation: gold microparticles > 20 µm and gold nanoparticles. The injection of gold microparticles (µGold) is a purely local therapy. µGold particles stay put where injected, and gold ions released from them are relatively few and taken up by cells within a sphere of only a few millimeters in diameter from their origin part icles. The macrophage-induced release of gold ions may continue for years. Injection...
Source: Histochemistry and Cell Biology - March 2, 2023 Category: Biomedical Science Source Type: research
Developmental function of Piezo1 in mouse submandibular gland morphogenesis
AbstractMechanically activated factors are important in organogenesis, especially in the formation of secretory organs, such as salivary glands. Piezo-type mechanosensitive ion channel component 1 (Piezo1), although previously studied as a physical modulator of the mechanotransduction, was firstly evaluated on its developmental function in this study. The detailed localization and expression pattern of Piezo1 during mouse submandibular gland (SMG) development were analyzed using immunohistochemistry and RT-qPCR, respectively. The specific expression pattern ofPiezo1 was examined in acinar-forming epithelial cells at embryo...
Source: Histochemistry and Cell Biology - February 22, 2023 Category: Biomedical Science Source Type: research
Air –liquid interface cultures trigger a metabolic shift in intestinal epithelial cells (IPEC-1)
Abstract
An improved oxygen availability in air –liquid interface (ALI) cultures of enterocytes of the small intestine seems to be primarily responsible for morphological, metabolic, and functional changes. Intestinal porcine epithelial cells 1 (IPEC-1) are less investigated and are rarely used as model for intestinal barrier but showed a profo und change of cell shape during ALI cultivation. We aim to answer the following question: Are the observed morphological effects accompanied by changes in metabolic function? A microarray analysis of submerged culture (SMC) and ALI cultures identified 830 significantly regulate...
Source: Histochemistry and Cell Biology - February 15, 2023 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 6, 2023 Category: Biomedical Science Source Type: research
