atoh8 expression pattern in early zebrafish embryonic development
AbstractAtonal homologue 8 (atoh8) is a basic helix-loop-helix transcription factor expressed in a variety of embryonic tissues. While several studies have implicatedatoh8 in various developmental pathways in other species, its role in zebrafish development remains uncertain. So far, no studies have dealt with an in-depth in situ analysis of the tissue distribution ofatoh8 in embryonic zebrafish. We set out to pinpoint the exact location ofatoh8 expression in a detailed spatio-temporal analysis in zebrafish during the first 24 h of development (hpf). To our surprise, we observed transcription from pre-segmentation stages...
Source: Histochemistry and Cell Biology - June 13, 2021 Category: Biomedical Science Source Type: research
The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells
In this study, we investigated the positional relationship between the microtubule nucleating sites and the Golgi apparatus in C2C12 myotubes and in primary cultured mouse skeletal myotubes. We focused on gaps in the perinuclear Golgi apparatus where the nuclear envelope was not covered with the Golgi apparatus. In microtubule regrowth assay, microtubule regrowth after cold-nocodazole depolymerization of preexisting microtubules was not found at the gap of the perinuclear Golgi apparatus. Most of the microtubule regrowth was detected at the CDK5RAP2 (CDK5 regulatory subunit-associated protein 2)-rich spots on the perinucle...
Source: Histochemistry and Cell Biology - June 10, 2021 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 7, 2021 Category: Biomedical Science Source Type: research
Identification of testicular Foxq1 as a critical modulator of lactate metabolism in mouse Sertoli cells
AbstractPostmeiotic germ cells require the lactate produced by the adjacent Sertoli cells (SCs) as their sole energy fuels. Lactate production in SCs is elaborately regulated by monitoring the transcription of the lactate dehydrogenase A (Ldha) gene. However, the transcription factors that are responsible for the control ofLdha transcription in SCs remain ill defined. Herein, the expression of forkhead box Q1 (FOXQ1), a central modulator of glucose metabolism in liver, was demonstrated in mouse testis throughout postnatal development, with maximum levels in adult specimens. At this age, FOXQ1 was immunolocalized in the nuc...
Source: Histochemistry and Cell Biology - June 5, 2021 Category: Biomedical Science Source Type: research
Effects of supraphysiological vitamin D3 (cholecalciferol) supplement on normal adult rat ovarian functions
In conclusion, treating VD-sufficient female rats with supraphysiological VD3 supplements was not associated with hypercalcaemia, and could contribute to ovarian functions by regulating the hypothalamic-pituitary-ovarian hormones and ovarian VD-related molecules. However, further studies are still needed to illustrate the clinical significance of VD3 in female reproduction. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 1, 2021 Category: Biomedical Science Source Type: research
