Comparative anatomy on the development of sperm transporting pathway between the testis and mesonephros
In conclusion, this study showed that mammals, avian species, and frogs commonly develop the bundle between the testis cords (testis canal) and the cluster of rete cells (lateral kidney canal), indicating that these animals share basic morphogenesis in the male genital tract. The connection site between the rete cells and mesonephric tubules is suggested to have changed from the nephrostome to the Bowman’s capsule during vertebrate evolution from anamniote to amniote. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 6, 2022 Category: Biomedical Science Source Type: research
Comparative microanatomical and histochemical biodistribution profiles of different types of mucins in oesophageal gastric tract mucosa of some tetrapod representatives
AbstractThe microanatomical features of the oesophageal gastric tract in tetrapod representatives and their function, especially those related to the mucosal layer, have not yet been fully investigated. The mucosal layer cells and their function in the oesophageal gastric tract differ structurally and functionally in tetrapod representatives based on interspecies difference and the type of food and feeding habits. The present study was, therefore, postulated to compare the mucosal microanatomical structure and histochemical biodistribution of different mucin types in oesophageal gastric tract tissues of four tetrapod speci...
Source: Histochemistry and Cell Biology - January 5, 2022 Category: Biomedical Science Source Type: research
Potassium permanganate is an excellent alternative to osmium tetroxide in freeze-substitution
AbstractHigh-pressure freezing followed by freeze-substitution is a valuable method for ultrastructural analyses of resin-embedded biological samples. The visualization of lipid membranes is one of the most critical aspects of any ultrastructural study and can be especially challenging in high-pressure frozen specimens. Historically, osmium tetroxide has been the preferred fixative and staining agent for lipid-containing structures in freeze-substitution solutions. However, osmium tetroxide is not only a rare and expensive material, but also volatile and toxic. Here, we introduce the use of a combination of potassium perma...
Source: Histochemistry and Cell Biology - January 5, 2022 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 4, 2022 Category: Biomedical Science Source Type: research
Correction to: Lung organoids: advances in generation and 3D-visualization
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - December 23, 2021 Category: Biomedical Science Source Type: research
Correction to: Measurements of transepithelial electrical resistance (TEER) are affected by junctional length in immature epithelial monolayers
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - December 23, 2021 Category: Biomedical Science Source Type: research
Alternative tissue fixation for combined histopathological and molecular analysis in a clinically representative setting
In this study, these criteria are assessed in a clinically representative setting. In total, 203 paired PAXgene Tissue and formalin-fixed samples were obtained. Blind-scored haematoxylin and eosin (H&E) sections showed comparable and acceptable staining. Immunohistochemistry (IHC) staining was suboptimal using existing protocols but improved with minor method adjustment and optimisation. Quality of DNA and RNA was significantly improved by PAXgene tissue fixation [RIN 2.8 versus 3.8 (p < 0.01), DIN 5.68 versus 6.77 (p < 0.001)], which translated into improved performance on qPCR assay. These results dem...
Source: Histochemistry and Cell Biology - December 14, 2021 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - December 2, 2021 Category: Biomedical Science Source Type: research
Insights into in vivo follicle formation: a review of in vitro systems
AbstractIn vitro systems capable of reconstituting the process of mouse oogenesis are now being established to help develop further understanding of the mechanisms underlying oocyte/follicle development and differentiation. These systems could also help increase the production of useful livestock or genetically modified animals, and aid in identifying the causes of infertility in humans. Recently, we revealed, using an in vitro system for recapitulating oogenesis, that the activation of the estrogen signaling pathway induces abnormal follicle formation, that blocking estrogen-induced expression of anti-M üllerian hormone ...
Source: Histochemistry and Cell Biology - November 30, 2021 Category: Biomedical Science Source Type: research
Galectin network in osteoarthritis: galectin-4 programs a pathogenic signature of gene and effector expression in human chondrocytes in vitro
AbstractGalectin-4 (Gal-4) is a member of the galectin family, which have been identified as galactose-binding proteins. Gal-4 possesses two tandem repeat carbohydrate recognition domains and acts as a cross-linking bridge in sulfatide-dependent glycoprotein routing. We herein document its upregulation in osteoarthritis (OA) in correlation with the extent of cartilage degradation in vivo. Primary human OA chondrocytes in vitro respond to carbohydrate-inhibitable Gal-4 binding with the upregulation of pro-degradative/-inflammatory proteins such as interleukin-1 β (IL-1β) and matrix metalloproteinase-13 (MMP-13), as docume...
Source: Histochemistry and Cell Biology - November 30, 2021 Category: Biomedical Science Source Type: research
Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts
AbstractThe incidence of cutaneous malignant melanoma is increasing worldwide. While the treatment of initial stages of the disease is simple, the advanced disease frequently remains fatal despite novel therapeutic options . This requires identification of novel therapeutic targets in melanoma. Similarly to other types of tumours, the cancer microenvironment plays a prominent role and determines the biological properties of melanoma. Importantly, melanoma cell-produced exosomes represent an important tool of intercellular communication within this cancer ecosystem. We have focused on potential differences in the activity o...
Source: Histochemistry and Cell Biology - November 27, 2021 Category: Biomedical Science Source Type: research
Activation of RIPK2-mediated NOD1 signaling promotes proliferation and invasion of ovarian cancer cells via NF- κB pathway
AbstractThe goal of this study was to investigate the role and mechanism of action of nucleotide oligomerization domain receptor 1 (NOD1) in ovarian cancer. Results showed that the expressions of NOD1 and receptor interacting serine/threonine kinase 2 (RIPK2) were notably upregulated in non-metastatic and metastatic ovarian tumors compared with matched non-tumor tissues, and their expression in metastatic tumor tissues was higher than that in non-metastatic tumors. Overexpression of NOD1 facilitated the expression of proliferation-related proteins (PCNA and Ki67) and proliferation and invasion of ovarian cancer cells. Over...
Source: Histochemistry and Cell Biology - November 26, 2021 Category: Biomedical Science Source Type: research
Focal adhesion kinase splicing and protein activation in papillary thyroid carcinoma progression
AbstractPapillary thyroid carcinoma (PTC), a common endocrine malignancy, presents a challenge from a prognostic standpoint. Molecular alterations underlying PTC progression include deregulation of focal adhesion kinase (FAK) at post-transcriptional and post-translational levels. Searching for candidate markers of PTC progression, we investigated the prognostic significance of FAK alterations on mRNA/protein level. The expression levels and subcellular localisation of auto-phosphorylated FAK (pY397-FAK) were determined by western blot (WB) and immunohistochemistry. The quantity of total FAK mRNA, alternatively spliced FAK-...
Source: Histochemistry and Cell Biology - November 24, 2021 Category: Biomedical Science Source Type: research
Age-related alterations of gastric mucosa and estrogen synthesis in rat parietal cells
AbstractThe stomach has diverse functions other than gastric acid secretion. Multifaceted studies have investigated age-related changes of the gastrointestinal tract. Nevertheless, little is known about estrogen production changes in gastric parietal cells in rats aged over 3 months. We investigated age-related changes in gastric estrogen synthesis and the accompanying changes in liver estrogen receptor from 3 to 24 months. Weights of the body, stomach, and liver increased linearly from 3 to 18 months, then maintained a constant proportion up to 24 months. The gastr ic mucosa area (in mm2/1 mm muscularis mucosa) sho...
Source: Histochemistry and Cell Biology - November 22, 2021 Category: Biomedical Science Source Type: research
Von Kossa and his staining technique
This article pays tribute to von Kossa and to his handy staining method. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - November 20, 2021 Category: Biomedical Science Source Type: research
