The secretome from human-derived mesenchymal stem cells augments the activity of antitumor plant extracts in vitro
AbstractCancer is understood as a multifactorial disease that involve multiple cell types and phenotypes in the tumor microenvironment (TME). The components of the TME can interact directly or via soluble factors (cytokines, chemokines, growth factors, extracellular vesicles, etc.). Among the cells composing the TME, mesenchymal stem cells (MSCs) appear as a population with debated properties since it has been seen that they can both promote or attenuate tumor progression. For various authors, the main mechanism of interaction of MSCs is through their secretome, the set of molecules secreted into the extracellular milieu, ...
Source: Histochemistry and Cell Biology - February 24, 2024 Category: Biomedical Science Source Type: research
Role of Wisteria floribunda agglutinin binding glycans in carcinogenesis and metastasis of cholangiocarcinoma
In conclusion, we have demonstrated the association of WFAG with carcinogenesis and metastasis of CCA, suggesting its potential as a target for the treatment of the disease. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 23, 2024 Category: Biomedical Science Source Type: research
Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation
In this study, we used three RMS cell lines (SJRH30, RD, and HS-729) to assess its expression profile, intracellular localization, and turnover. They express wild-type ANKRD1, as judged by the sequencing of the open reading frame. Each cell line expressed a different amount of ANKRD1 protein, although the transcript level was similar. According to western blot analysis, ANKRD1 protein was expressed at detectable levels in the SJRH30 and RD cells (SJRH30 > RD), but not in the HS-729, even after immunoprecipitation. Immunocytochemistry revealed nuclear and cytoplasmic localization of ANKRD1 in all examined cell line...
Source: Histochemistry and Cell Biology - February 23, 2024 Category: Biomedical Science Source Type: research
The impact of apelin-13 on cisplatin-induced endocrine pancreas damage in rats: an in vivo study
This study aims to investigate the potential protective effects of apelin-13 in cisplatin-induced endocrine pancreatic damage. Twenty-four rats were divided into four groups: control, apelin-13, cisplatin, and cisplatin + apelin-13. Caspase-3, TUNEL, and Ki-67 immunohistochemical staining were used as markers of apoptosis and mitosis. NF-κB/p65 and TNFα were used to show inflammation. β-cells and α-cells were also evaluated with insulin and glucagon staining in the microscopic examination. Pancreatic tissu e was subjected to biochemical analyses of glutathione (GSH) and malondialdehyde (MDA). Apelin-13 ameliorated...
Source: Histochemistry and Cell Biology - February 18, 2024 Category: Biomedical Science Source Type: research
Fibrosis and expression of extracellular matrix proteins in human interventricular septum in aortic valve stenosis and regurgitation
AbstractValvular heart disease leads to ventricular pressure and/or volume overload. Pressure overload leads to fibrosis, which might regress with its resolution, but the limits and details of this reverse remodeling are not known. To gain more insight into the extent and nature of cardiac fibrosis in valve disease, we analyzed needle biopsies taken from the interventricular septum of patients undergoing surgery for valve replacement focusing on the expression and distribution of major extracellular matrix protein involved in this process. Proteomic analysis performed using mass spectrometry revealed an excellent correlati...
Source: Histochemistry and Cell Biology - February 12, 2024 Category: Biomedical Science Source Type: research
Effectiveness of cannabidiol (CBD) on histopathological changes and gene expression in hepatocellular carcinoma (HCC) model in male rats: the role of Hedgehog (Hh) signaling pathway
In conclusion, by focusing on the Hh signaling system, administration of CBD showed a beneficial improvement in the liver enzymes, oxidant–antioxidant status, morphological, and molecular parameters in the DENA-induced HCC in adult male rats. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 1, 2024 Category: Biomedical Science Source Type: research
Addressing radiotherapy-induced fibrosis: the potential of platelet-rich plasma and infliximab for improved breast cancer management
This study provides insights into optimizing outcomes i n reconstructive breast surgery, paving the way for further research and clinical studies. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 28, 2024 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 24, 2024 Category: Biomedical Science Source Type: research
The peroxisome: an update on mysteries 3.0
AbstractPeroxisomes are highly dynamic, oxidative organelles with key metabolic functions in cellular lipid metabolism, such as the β-oxidation of fatty acids and the synthesis of myelin sheath lipids, as well as the regulation of cellular redox balance. Loss of peroxisomal functions causes severe metabolic disorders in humans. Furthermore, peroxisomes also fulfil protective roles in pathogen and viral defence and immunity, hig hlighting their wider significance in human health and disease. This has sparked increasing interest in peroxisome biology and their physiological functions. This review presents an update and a co...
Source: Histochemistry and Cell Biology - January 20, 2024 Category: Biomedical Science Source Type: research
Studying the topology of peroxisomal acyl-CoA synthetases using self-assembling split sfGFP
AbstractPeroxisomes are membrane-bounded organelles that contain enzymes involved in multiple lipid metabolic pathways. Several of these pathways require (re-)activation of fatty acids to coenzyme A (CoA) esters by acyl-CoA synthetases, which may take place inside the peroxisomal lumen or extraperoxisomal. The acyl-CoA synthetases SLC27A2, SLC27A4, ACSL1, and ACSL4 have different but overlapping substrate specificities and were previously reported to be localized in the peroxisomal membrane in addition to other subcellular locations. However, it has remained unclear if the catalytic acyl-CoA synthetase sites of these enzym...
Source: Histochemistry and Cell Biology - January 19, 2024 Category: Biomedical Science Source Type: research
JNK inhibition enhances cell –cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes
Abstractc-Jun NH2-terminal protein kinase (JNK) and p38 are stress-activated mitogen-activated protein kinases (MAPK) that are phosphorylated by various stimuli. It has been reported that the loss of desmoglein (DSG) 3, a desmosomal transmembrane core molecule, in keratinocytes impairs cell –cell adhesion accompanied by p38 MAPK activation. To understand the biological role of DSG3 in desmosomes and its relationship with stress-activated MAPKs, we established DSG3 knockout keratinocytes (KO cells). Wild-type cells showed a linear localization of DSG1 to cell–cell contacts, whereas KO cells showed a remarkable reduction...
Source: Histochemistry and Cell Biology - January 16, 2024 Category: Biomedical Science Source Type: research
The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury
In this study, we investigated the role of SETDB1 in epithelial regeneration using an intestinal ischemia/reperfusion injury (IRI) mouse model. Jejunum tissues were sampled after 75 min of ischemia followed by 3, 24, and 48 h of reperfusion. Morphological evaluations were performed using light microscopy and electron microscopy, and the involvement of SETDB1 in epithelial remodeling was investigated by immunohistochemistry. Expression of SETDB1 was increased following 24 h of reperfusion and localized in not only the crypt bottom but also in the transit amplifying zone and part of the villi. Changes in cell lineage, rep...
Source: Histochemistry and Cell Biology - January 13, 2024 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 9, 2024 Category: Biomedical Science Source Type: research
From complexity to clarity: unravelling tumor heterogeneity through the lens of tumor microenvironment for innovative cancer therapy
AbstractDespite the tremendous clinical successes recorded in the landscape of cancer therapy, tumor heterogeneity remains a formidable challenge to successful cancer treatment. In recent years, the emergence of high-throughput technologies has advanced our understanding of the variables influencing tumor heterogeneity beyond intrinsic tumor characteristics. Emerging knowledge shows that drivers of tumor heterogeneity are not only intrinsic to cancer cells but can also emanate from their microenvironment, which significantly favors tumor progression and impairs therapeutic response. Although much has been explored to under...
Source: Histochemistry and Cell Biology - January 8, 2024 Category: Biomedical Science Source Type: research
Insulin granule morphology and crinosome formation in mice lacking the pancreatic β cell-specific phogrin (PTPRN2) gene
In this study, we performed a comprehensive analysis of morphological changes inRIP-Cre+/ −Phogrinflox/flox (βKO) mice with β cell-specific phogrin gene knockout. Compared to controlRIP-Cre+/ −Phogrin+/+ (Ctrl) mice, agedβKO mice exhibited a decreased density of insulin granules, which can be categorized into three subtypes. While no differences were observed in the density and size of lysosomes and crinosomes, organelles involved in insulin granule reduction, significant alterations in the regions of lysosomes responding positively to carbohydrate labeling were evident in youngβKO mice. These alterations differed ...
Source: Histochemistry and Cell Biology - December 27, 2023 Category: Biomedical Science Source Type: research
