NADH intraperitoneal injection prevents massive pancreatic beta cell destruction in a streptozotocin-induced diabetes in rats
AbstractDiabetes mellitus is a chronic metabolic disease characterized by persistent hyperglycemia, revealing a decrease in insulin efficiency. The sustained glucotoxic pancreatic microenvironment increases reactive oxygen species generation, resulting in chronic oxidative stress responsible for massive DNA damage. This triggers PARP-1 activation with both NAD+ and ATP depletion, affecting drastically pancreatic beta cells ’ energy storage and leading to their dysfunction and death. The aim of the present study is to highlight the main histological changes observed in pancreatic islets pre-treated with a unique NADH intr...
Source: Histochemistry and Cell Biology - November 9, 2023 Category: Biomedical Science Source Type: research
Revisiting the development of cerebellar inhibitory interneurons in the light of single-cell genetic analyses
AbstractThe present review aims to provide a short update of our understanding of the inhibitory interneurons of the cerebellum. While these cells constitute but a minority of all cerebellar neurons, their functional significance is increasingly being recognized. For one, inhibitory interneurons of the cerebellar cortex are now known to constitute a clearly more diverse group than their traditional grouping as stellate, basket, and Golgi cells suggests, and this diversity is now substantiated by single-cell genetic data. The past decade or so has also provided important information about interneurons in cerebellar nuclei. ...
Source: Histochemistry and Cell Biology - November 8, 2023 Category: Biomedical Science Source Type: research
Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life
AbstractCell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we fo...
Source: Histochemistry and Cell Biology - November 8, 2023 Category: Biomedical Science Source Type: research
Local fractal dimension of collagen detects increased spatial complexity in fibrosis
AbstractIncrease of collagen content and reorganization characterizes fibrosis but quantifying the latter remains challenging. Spatially complex structures are often analyzed via the fractal dimension; however, established methods for calculating this quantity either provide a single dimension for an entire object or a spatially distributed dimension that only considers binary images. These neglect valuable information related to collagen density in images of fibrotic tissue. We sought to develop a fractal analysis that can be applied to 3-dimensional (3D) images of fibrotic tissue. A fractal dimension map for each image w...
Source: Histochemistry and Cell Biology - November 8, 2023 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - October 31, 2023 Category: Biomedical Science Source Type: research
Editorial: Histochemistry and Cell Biology implements new submission guidelines for image presentation and image analysis
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - October 25, 2023 Category: Biomedical Science Source Type: research
Early growth response 2, a novel target of pelvic organ prolapse, is highly expressed in anterior vaginal wall tissues with pelvic organ prolapse
This study might provide a new insight into the pathogenesis of POP. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - October 24, 2023 Category: Biomedical Science Source Type: research
The histomorphological and stereological assessment of rat dorsal root ganglion tissues after various types of sciatic nerve injury
AbstractPeripheral nerve injuries lead to significant changes in the dorsal root ganglia, where the cell bodies of the damaged axons are located. The sensory neurons and the surrounding satellite cells rearrange the composition of the intracellular organelles to enhance their plasticity for adaptation to changing conditions and response to injury. Meanwhile, satellite cells acquire phagocytic properties and work with macrophages to eliminate degenerated neurons. These structural and functional changes are not identical in all injury types. Understanding the cellular response, which varies according to the type of injury in...
Source: Histochemistry and Cell Biology - October 19, 2023 Category: Biomedical Science Source Type: research
A note to our authors: Histochemistry and Cell Biology implements guidelines for the use of large language models (including ChatGPT)
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - October 19, 2023 Category: Biomedical Science Source Type: research
Mesenchymal stem cell transplantation in burn wound healing: uncovering the mechanisms of local regeneration and tissue repair
AbstractBurn injuries pose a significant healthcare burden worldwide, often leading to long-term disabilities and reduced quality of life. To explore the impacts of the transplantation of mesenchymal stem cells (MSCs) on the healing of burns and the levels of serum cytokines, 60 fully grown Sprague –Dawley rats were randomly divided into three groups (n = 20 each): group I (control), group II (burn induction), and group III (burn induction + bone marrow (BM)-MSC transplantation). Groups II and III were further divided into four subgroups (n = 5 each) based on euthanasia duration (7, 14, 21, and 28 days p...
Source: Histochemistry and Cell Biology - October 17, 2023 Category: Biomedical Science Source Type: research
Protective role of estrogen through G-protein coupled receptor 30 in a colitis mouse model
AbstractEstrogen and its receptors are involved in the pathogenesis of gastrointestinal diseases such as colitis. However, the role of the membrane estrogen receptor G-protein-coupled receptor 30 (GPR30) in colitis is poorly understood. We therefore investigated the effect of estrogen in dextran sulfate sodium (DSS)-induced colitis. Male C57BL/6 mice were administered 1.5% DSS for 5 days and treated with 17β-estradiol (E2), GPR30 agonist (G1), or GPR30 antagonist (G15) for 8 days. Inflammation grade was evaluated by disease activity index (DAI) and histomorphological score. Colon tissues were immunohistochemically ana...
Source: Histochemistry and Cell Biology - October 12, 2023 Category: Biomedical Science Source Type: research
Effects of royal jelly on the antisenescence, mitochondrial viability and osteogenic differentiation capacity of umbilical cord-derived mesenchymal stem cells
AbstractMesenchymal stem cells (MSCs) are multipotent cells that have the ability to self-renew and regulate paracrine signalling and immune system processes. MSCs have extensive clinical applications in regeneration, functional reconstruction and cellular therapies. However, studies are needed to discover ways to improve the properties of MSCs, such as differentiation, and prevent senescence in culture, which are both very important for cell therapies. Royal jelly (RJ) is a nutritional substance produced by worker bees that contains a substantial amounts of proteins that are beneficial for cell growth and proliferation. R...
Source: Histochemistry and Cell Biology - October 9, 2023 Category: Biomedical Science Source Type: research
Labour and premature delivery differentially affect the expression of the endocannabinoid system in the human placenta
AbstractPlasma concentrations ofN-arachidonyletholamine (AEA),N-oleoylethanolamide (OEA) andN-palmitoylethanolamide (PEA) increase at term and can predict when a woman is likely to go into labour. We hypothesised that increased plasma AEA concentrations in women in preterm and term labour might also be increased and have a function in the placenta at the end of pregnancy. Here we examined the expression of theN-acylethanolamine-modulating enzymes fatty acid amide hydrolase (FAAH) andN-acyl-phosphatidylethanolamine-specific phospholipase-D (NAPE-PLD) and of the cannabinoid receptors (CB1 and CB2) in the placenta and their a...
Source: Histochemistry and Cell Biology - September 26, 2023 Category: Biomedical Science Source Type: research
Non-canonical DNA structures in the human ribosomal DNA
AbstractNon-canonical structures (NCS) refer to the various forms of DNA that differ from the B-conformation described by Watson and Crick. It has been found that these structures are usual components of the genome, actively participating in its essential functions. The present review is focused on the nine kinds of NCS appearing or likely to appear in human ribosomal DNA (rDNA): supercoiling structures, R-loops, G-quadruplexes, i-motifs, DNA triplexes, cruciform structures, DNA bubbles, and A and Z DNA conformations. We discuss the conditions of their generation, including their sequence specificity, distribution within t...
Source: Histochemistry and Cell Biology - September 26, 2023 Category: Biomedical Science Source Type: research
HK-2 cell response to TGF- β highly depends on cell culture medium formulations
In conclusion, our study demonstrates that, using the HK-2 in vitro model of EMT, the meticulous selection of appropriate cell culture medium formulation is essential to achieve reliable scientific results. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - September 26, 2023 Category: Biomedical Science Source Type: research
