EBF1 is expressed in pericytes and contributes to pericyte cell commitment
AbstractEarly B-cell factor-1 (EBF1) is a transcription factor with an important role in cell lineage specification and commitment during the early stage of cell maturation. Originally described during B-cell maturation, EBF1 was subsequently identified as a crucial molecule for proper cell fate commitment of mesenchymal stem cells into adipocytes, osteoblasts and muscle cells. In vessels, EBF1 expression and function have never been documented. Our data indicate that EBF1 is highly expressed in peri-endothelial cells in both tumor vessels and in physiological conditions. Immunohistochemistry, quantitative reverse transcri...
Source: Histochemistry and Cell Biology - July 16, 2021 Category: Biomedical Science Source Type: research
Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro
In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 16, 2021 Category: Biomedical Science Source Type: research
Delayed maturation of thymic epithelium in mice with specific deletion of β-catenin gene in FoxN1 positive cells
AbstractWnt signalling pathways have been reported to be involved in thymus development but their precise role in the development of both thymic epithelium (TE) and thymocytes is controversial. Herein, we examined embryonic, postnatal and adult thymi of mice with a specific deletion of β-catenin gene in FoxN1+ thymic epithelial cells (TECs). Together with a high postnatal mouse mortality, the analysis showed severe thymic hypocellularity, largely due an important reduction in numbers of developing thymocytes, and delayed, partially blocked maturation of mutant TECs. Affected TECs included largely cortical (c) TEC subsets,...
Source: Histochemistry and Cell Biology - July 12, 2021 Category: Biomedical Science Source Type: research
In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 7, 2021 Category: Biomedical Science Source Type: research
Correction to: Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 7, 2021 Category: Biomedical Science Source Type: research
Retraction Note to: XBP1S protects cells from ER stress-induced apoptosis through Erk1/2 signaling pathway involving CHOP
A correction to this paper has been published: https://doi.org/10.1007/s00418-021-02000-0 (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 1, 2021 Category: Biomedical Science Source Type: research
CD9-positive cells in the intermediate lobe migrate into the anterior lobe to supply endocrine cells
AbstractThe adenohypophysis is composed of the anterior and intermediate lobes (AL and IL), and secretes important hormones for growth, sexual development, metabolism, and reproduction. In the marginal cell layer (MCL) facing Rathke ’s cleft between the IL and AL, cluster of differentiation (CD) 9-, CD81-, S100β-, and SOX2-quadruple positive (CD9/CD81/S100β/SOX2-positive) cells in the adult IL are settled as tissue-resident stem/progenitor cells supplying hormone-producing cells to the AL. However, it is unclear how CD9/CD8 1/S100β/SOX2-positive cells in the IL-side MCL migrate into the AL across Rathke’s cleft. In ...
Source: Histochemistry and Cell Biology - June 29, 2021 Category: Biomedical Science Source Type: research
Reprogramming of DNA methylation is linked to successful human preimplantation development
In conclusion, our results suggest that DNA methylation reprogramming is conserved in humans, with human-specific dynamics and extent. Furthermore, abnormalities in the four-cell-specific DNA methylome in early human embryogenesis are associated with abnormal development, highlighting an essential role of epigenetic reprogramming for successful human embryogenesis. Further research should identify the underlying genomic regions and cause of abnormal DNA methylation reprogramming in early human embryos. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 27, 2021 Category: Biomedical Science Source Type: research
Classification of human enteric neurons
AbstractMajor advances in our understanding of the functional heterogeneity of enteric neurons are driven by the application of newly developed, innovative methods. In contrast to this progress, both animal and human enteric neurons are usually divided into only two morphological subpopulations, “Dogiel type II” neurons (with several long processes) and “Dogiel type I” neurons (with several short processes). This implies no more than the distinction of intrinsic primary afferent from all other enteric neurons. The well-known chemical and functional diversity of enteric neurons is no t reflected by this restrictive ...
Source: Histochemistry and Cell Biology - June 25, 2021 Category: Biomedical Science Source Type: research
Interaction between cadherins, vimentin, and V-set and immunoglobulin domain containing 1 in gastric-type hepatocellular carcinoma
We examined VSIG1 immunohistochemical (IHC) expression in 105 consecutive samples provided by HCC patients, along with the IHC expression of three of the biomarkers known to be involved in the epithelial –mesenchymal transition (EMT): vimentin (VIM), and E- and N-cadherin (encoded by CDH1 and CDH2 genes). IHC subcellular localization of thyroid transcription factor 1 (TTF1), in which nuclear-to-cytoplasmic translocation is known to cause a lineage shift from lung to gastric-type adenocarcinoma, wa s also checked. The obtained data were validated using the miRNET program. In the examined HCC samples, VSIG1 expression was ...
Source: Histochemistry and Cell Biology - June 25, 2021 Category: Biomedical Science Source Type: research
Flow-through isolation of human first trimester umbilical cord endothelial cells
AbstractHuman umbilical vein and artery endothelial cells (HUVEC; HUAEC), placental endothelial cells (fpAEC), and endothelial colony-forming cells (ECFC) from cord blood are a widely used model for researching placental vascular development, fetal and placental endothelial function, and the effect of adverse conditions in pregnancy thereon. However, placental vascular development and angiogenesis start in the first weeks of gestation, and adverse conditions in pregnancy may also affect endothelial function before term, suggesting that endothelial cells from early pregnancy may respond differently. Thus, we established a n...
Source: Histochemistry and Cell Biology - June 24, 2021 Category: Biomedical Science Source Type: research
Nuclear expression of VDR and AHR is mutually exclusive in glandular cells in endometriosis
AbstractThe vitamin D receptor (VDR) and aryl hydrocarbon receptor (AHR) are two nuclear receptors that exert their effects by binding with ligands and forming a molecular complex. These complexes translocate to the nucleus and activate the expression of a series of genes which have a response element to VDR or AHR. Both receptors have been identified in the pathogenesis of endometriosis, a common disease characterized by the formation of endometrium-like tissue in ectopic zones. Despite numerous therapies, there is no definitive cure for endometriosis at the pharmacological level. Our study aims to describe the location a...
Source: Histochemistry and Cell Biology - June 21, 2021 Category: Biomedical Science Source Type: research
Imitating evolution ’s tinkering by protein engineering reveals extension of human galectin-7 activity
This study provides a clear direction for research on defining the full range of Gal-7 functionality and offers the perspective of testing applications for engineered heterodimers. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 21, 2021 Category: Biomedical Science Source Type: research
Correction to: Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain
A correction to this paper has been published: https://doi.org/10.1007/s00418-021-01997-8 (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 18, 2021 Category: Biomedical Science Source Type: research
Subcellular dynamics of estrogen-related receptors involved in transrepression through interactions with scaffold attachment factor B1
AbstractEstrogen-related receptor (ERR), a member of the nuclear receptor superfamily, consists of three subtypes ( α, β, γ) and has strong homology with estrogen receptor. No endogenous ligands have been identified for ERRs, but they play key roles in metabolic, hormonal, and developmental processes as transcription factors without ligand binding. Although subnuclear dynamics are essential for nuclear events including nuclear receptor-mediated transcriptional regulation, the dynamics of ERRs are poorly understood. Here, we report that ERRs show subcellular kinetic changes in response to diethylstilbestrol (DES), a synt...
Source: Histochemistry and Cell Biology - June 15, 2021 Category: Biomedical Science Source Type: research
