In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 20, 2022 Category: Biomedical Science Source Type: research
Ameloblastoma modifies tumor microenvironment for enhancing invasiveness by altering collagen alignment
In conclusion, ameloblastoma-mediated ECM remodeling contributes to the formation of an invasive collagen architecture during tumor progression. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 20, 2022 Category: Biomedical Science Source Type: research
Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus
AbstractWe previously reported that the membrane skeletal protein 4.1G in the peripheral nervous system transports membrane palmitoylated protein 6 (MPP6), which interacts with the synaptic scaffolding protein Lin7 and cell adhesion molecule 4 (CADM4) in Schwann cells that form myelin. In the present study, we investigated the localization of and proteins related to MPP2, a highly homologous family protein of MPP6, in the cerebellum of the mouse central nervous system, in which neurons are well organized. Immunostaining for MPP2 was observed at cerebellar glomeruli (CG) in the granular layer after postnatal day 14. Using t...
Source: Histochemistry and Cell Biology - July 19, 2022 Category: Biomedical Science Source Type: research
GLAST versus GFAP as astroglial marker for the subcellular study of cannabinoid CB1 receptors in astrocytes
AbstractThe cannabinoid CB1 receptor-mediated functions in astrocytes are highly dependent on the CB1 receptor distribution in these glial cells relative to neuronal sites, particularly at the nearby synapses under normal or pathological conditions. However, the portrait of the CB1 receptor distribution in astroglial compartments remains uncompleted because of the scarce CB1 receptor expression in these cells and the limited identification of astrocytes. The glial fibrillary acidic protein (GFAP) is commonly used as astroglial marker. However, because GFAP is a cytoskeleton protein mostly restricted to the astroglial cell ...
Source: Histochemistry and Cell Biology - July 19, 2022 Category: Biomedical Science Source Type: research
Combined resveratrol and vitamin D treatment ameliorate inflammation-related liver fibrosis, ER stress, and apoptosis in a high-fructose diet/streptozotocin-induced T2DM model
In conclusion, this study indicates that the combin ation treatment of resveratrol + VitD can be a beneficial option for preventing liver damage in fructose-induced T2DM. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 18, 2022 Category: Biomedical Science Source Type: research
Immunolocalization of zinc transporters and metallothioneins reveals links to microvascular morphology and functions
This study aimed to assess protein expression and localization of zinc transporters of the SLC39A/ZIP family (ZIPs) and metallothioneins (MTs) in human subcutaneous microvessels and to relate them to morphological features and expression of function-related molecu les in the microvasculature. Microvessels in paraffin biopsies of subcutaneous adipose tissues from 14 patients undergoing hernia reconstruction surgery were analysed for 9 ZIPs and 3 MT proteins by MQCM (multifluorescence quantitative confocal microscopy). Zinc regulation proteins detected in human microvasculature included ZIP1, ZIP2, ZIP8, ZIP10, ZIP12, ZIP14 ...
Source: Histochemistry and Cell Biology - July 18, 2022 Category: Biomedical Science Source Type: research
Micro-scale assessment of bone quality changes in adult cadaveric men with congestive hepatopathy
AbstractCongestive hepatopathy (CH) is a chronic liver disease (CLD) caused by impaired hepatic venous blood outflow, most frequently resulting from congestive heart failure. Although it is known that heart failure and CLDs contribute to increased risk for age-related fractures, an assessment of CH-induced skeletal alterations has not been made to date. The aim of our study was to characterize changes in bone quality in adult male cadavers with pathohistologically confirmed CH compared with controls without liver disease. The anterior mid-transverse part of the fifth lumbar vertebral body was collected from 33 adult male...
Source: Histochemistry and Cell Biology - July 18, 2022 Category: Biomedical Science Source Type: research
The changing landscape of immune cells in the fetal mouse testis
This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/80+ cells were the most abundant, comprising 90% of CD45+ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented ...
Source: Histochemistry and Cell Biology - July 12, 2022 Category: Biomedical Science Source Type: research
Megapinosomes and homologous structures in hematopoietic cells
AbstractMegapinosomes are endocytic organelles found in human macrophage colony-stimulating factor (M-CSF) monocyte-derived M macrophages. They are large (several microns) and have a complex internal structure that is connected with the cytosol and consists of interconnected knots and concave bridges with sizes in the range of 100 nm. We called this structure trabecular meshwork. The luminal part of the megapinosome can be connected with luminal tubules and cisterns that form the megapinosome complex. The structures are especially well visible in scanning electron tomography when macrophages are prepared by high-pressur...
Source: Histochemistry and Cell Biology - July 12, 2022 Category: Biomedical Science Source Type: research
Three-dimensional SEM, TEM, and STEM for analysis of large-scale biological systems
AbstractA major aim in structural cell biology is to analyze intact cells in three dimensions, visualize subcellular structures, and even localize proteins at the best possible resolution in three dimensions. Though recently developed electron microscopy tools such as electron tomography, or three-dimensional (3D) scanning electron microscopy, offer great resolution in three dimensions, the challenge is that, the better the resolution, usually the smaller the volume under investigation. Several different approaches to overcome this challenge were presented at the Microscopy Conference in Vienna in 2021. These tools include...
Source: Histochemistry and Cell Biology - July 12, 2022 Category: Biomedical Science Source Type: research
A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology
AbstractThe anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protoc...
Source: Histochemistry and Cell Biology - July 9, 2022 Category: Biomedical Science Source Type: research
Embryonic nutritional hyperglycemia decreases cell proliferation in the zebrafish retina
AbstractDiabetic retinopathy (DR) is one of the leading causes of blindness in the world. While there is a major focus on the study of juvenile/adult DR, the effects of hyperglycemia during early retinal development are less well studied. Recent studies in embryonic zebrafish models of nutritional hyperglycemia (high-glucose exposure) have revealed that hyperglycemia leads to decreased cell numbers of mature retinal cell types, which has been related to a modest increase in apoptotic cell death and altered cell differentiation. However, how embryonic hyperglycemia impacts cell proliferation in developing retinas still rema...
Source: Histochemistry and Cell Biology - July 2, 2022 Category: Biomedical Science Source Type: research
The Golgi complex: An organelle that determines urothelial cell biology in health and disease
AbstractThe Golgi complex undergoes considerable structural remodeling during differentiation of urothelial cells in vivo and in vitro. It is known that in a healthy bladder the differentiation from the basal to the superficial cell layer leads to the formation of the tightest barrier in our body, i.e., the blood –urine barrier. In this process, urothelial cells start expressing tight junctional proteins, apical membrane lipids, surface glycans, and integral membrane proteins, the uroplakins (UPs). The latter are the most abundant membrane proteins in the apical plasma membrane of differentiated superficia l urothelial c...
Source: Histochemistry and Cell Biology - June 30, 2022 Category: Biomedical Science Source Type: research
Landmark-based retrieval of inflamed skin vessels enabled by 3D correlative intravital light and volume electron microscopy
AbstractThe nanometer spatial resolution of electron microscopy imaging remains an advantage over light microscopy, but the restricted field of view that can be inspected and the inability to visualize dynamic cellular events are definitely drawbacks of standard transmission electron microscopy (TEM). Several methods have been developed to overcome these limitations, mainly by correlating the light microscopical image to the electron microscope with correlative light and electron microscopy (CLEM) techniques. Since there is more than one method to obtain the region of interest (ROI), the workflow must be adjusted according...
Source: Histochemistry and Cell Biology - June 29, 2022 Category: Biomedical Science Source Type: research
Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering
In this study, we investigated the effects of an intermittent ELF-EMF exposure (6 h/day) for the standard differentiation period (28 days) and for 10 days in hPDLSCs in the presence or not of osteogenic differentiation medium (OM). We evaluated cell proliferation, de novo calcium deposition and osteogenic differentiation marker expression in sham and ELF-EMF-exposed cells. Af ter ELF-EMF exposure, compared with sham-exposed, an increase in cell proliferation rate (p < 0.001) and de novo calcium deposition (p < 0.001) was observed after 10 days of exposure. Real-time PCR and Western blot results showed ...
Source: Histochemistry and Cell Biology - June 25, 2022 Category: Biomedical Science Source Type: research
