Forensic Science International: Genetics Supplement Series This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

WITHDRAWN: Cutaneous miRNAs expression in human hanging injuries
This article has been withdrawn at the request of the author(s) and editor. The Publisher apologizes for any inconvenience this may cause.The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 29, 2019 Category: Forensic Medicine Authors: M. Fabbri, P. Frisoni, M. Marti, L. Alfieri, E. Calabrese, C. Marini, R. Marino, R.M. Gaudio, M. Neri Source Type: research

The EUROFORGEN NAME Ampliseq ™ custom panel: A second tier panel developed for differentiation of individuals from the Middle East/North Africa
The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq ™ custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5™ (Thermo Fisher Scientific). (Source: Forensic Scienc...
Source: Forensic Science International: Genetics Supplement Series - October 29, 2019 Category: Forensic Medicine Authors: D.M. Truelsen, V. Pereira, C. Phillips, N. Morling, C. B ørsting Source Type: research

SlimDna � An in-house expert system for STR profiling
At NFC the internally developed software for DNA analysis, SlimDna is used. It is complementary to the LIMS and keeps track of samples, sample batches, instrument files and generated data. The system communicates with the LIMS, instruments and other software. SlimDna includes four modules (Analysis, Control, Administration and Frequency calculation) and covers the entire analysis process from DNA extraction to reporting approved STR profiles to the LIMS. SlimDna is built with Microsoft Internet Information Services (IIS) as application server and the user interface consists of Windows clients. (Source: Forensic Science Int...
Source: Forensic Science International: Genetics Supplement Series - October 28, 2019 Category: Forensic Medicine Authors: Malin Sanga, Linus Ek, Samuel Boiso Source Type: research

Preliminary exploration of a novel method for the deconvolution of DNA mixtures by pyrosequencing
In this study, we propose a novel method for the analysis of DNA mixtures. This method employed multiplex pyrosequencing to sequence several selected microhaplotypes. Employing a sparse representation-based AdvISER-MH-PYRO algorithm, the original pyrosequencing (PSQ) signals of the DNA mixtures were deciphered to determine the contributors of the mixtures. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 27, 2019 Category: Forensic Medicine Authors: Yueyan Cao, Qiuyue Wang, Qiang Zhu, Yuguo Huang, Yuhan Hu, Yijun Zhou, Yufang Wang, Ji Zhang Source Type: research

SlimDna – an in-house expert system for STR profiling
At NFC the internally developed software for DNA analysis, SlimDna is used. It is complementary to the LIMS and keeps track of samples, sample batches, instrument files and generated data. The system communicates with the LIMS, instruments and other software. SlimDna includes four modules (Analysis, Control, Administration and Frequency calculation) and covers the entire analysis process from DNA extraction to reporting approved STR profiles to the LIMS. SlimDna is built with Microsoft Internet Information Services (IIS) as application server and the user interface consists of Windows clients. (Source: Forensic Science Int...
Source: Forensic Science International: Genetics Supplement Series - October 27, 2019 Category: Forensic Medicine Authors: Malin Sanga, Linus Ek, Samuel Boiso Source Type: research

Internal validation of latest generation str kits for direct str typing from reference samples
Forensic STR kits are manufactured by various biotechnology companies. Differences in primer designs and proprietary chemicals, as well as the availability of instruments in each laboratory, may result in performance differences. As such, internal validation is a necessary step prior to adopting a new kit, especially if accreditation is sought. Here, we evaluated three latest generation direct STR kits (Thermo Fisher Scientific ’s GlobalFiler® Express, Promega’s PowerPlex® Fusion 6C, and Qiagen’s Investigator® 24plex GO!) for direct STR typing of reference samples as part of ISO17025:2017 implementation. (Source: ...
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: Wannarat Nongmanee, Pirunpat Aitthiprajak, Sukanya Phetpeng, Auttapol Viset, Arjaree Ujchariyakajorn, Nuryanee Sabahanaloh, Sakawrat Aoumtas, Thitika Kitpipit, Phuvadol Thanakiatkrai Source Type: research

Efficient preservation of DNA extracted from blood in FTA cards by Chelex method
Chelex resin has been developed and widely used for extracting DNA from different forensic-type samples [1]. However, no studies have shown the conservation of the extracted samples over time in samples from buccal swabs and blood in FTA cards. For this study, we tested three DNA extraction methods: Chelex 10%-Proteinase K (CHK), Phenol Chloroform (PC) and Chelex 10%-Proteinase K purified with Phenol Chloroform (CHK + PC) in two different kind of samples: blood stored over FTA cards and buccal swab samples. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: G. Burgos, R. Flores-Espinoza, V.A. Ruiz-Pozo, I. Villacr és Granda Source Type: research

Degraded dna typing highly improved by a codis-core + ess megaplex based on superprimers
On degraded DNA samples a multiplex PCR mixture containing polynucleotide primers can provide superior genotyping of Short Tandem Repeats (STRs) to the current commercial kits based on standard oligonucleotides. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: Martin E. Mautner, Mariela Caputo, Santiago Ginart, Tatiana S. Bengochea, Daniel Corach Source Type: research

Forensic application of a mtDNA minisequencing 52plex: tracing maternal lineages in Spanish Civil War remains
In this study we report the application of a minisequencing panel of 52 SNPs of the mtDNA, which allows the determination of the major world-wide mitochondrial haplogroups, in highly degraded samples. A total of 25 human remains from a mass grave of the Spanish Civil War (1936-1939) were analyzed in order to define their corresponding mtDNA haplogroup and, subsequently, investigate possible maternal relationships with the available reference samples. The results highlight the potential of this 52 mtSNP panel as an efficient screening method that can be used as an alternative or complementary approach to mtDNA sequencing in...
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: Miriam Baeta, Sandra Garc ía-Rey, Leire Palencia-Madrid, Caterina Raffone, Marian M de Pancorbo Source Type: research

Prevalence of DNA in vehicles: Persistence of DNA from a temporary driver after reuse by its regular driver on items less commonly targeted
This study investigates the yields and profiles of DNA recovered from several sites not usually sampled in a car, after a car with a known history was driven for ∼30 min by a known temporary driver and then sampled immediately, or after different durations of further use by its regular driver. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: T. Boyko, B. Kokshoorn, A.H. van Oorschot Roland Source Type: research

Analysis of forensic casework samples by Precision ID Ancestry panel � manual and automated Ampliseq workflow
In this study we compared library construction using the automated workflow with the Ion Chef and a manual workflow. Casework samples that were collected between 2005 and 2018 in the State of Qatar where used for the study and the samples varied from routine stains to challenging samples that yielded full and partial STR profiles.One hundred and forty-three samples were processed: sixteen using both workflows. Full and partial profiles were seen with both methods. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: Waad Rashed Al-Dosari, Ibrahim Abdullah Al-Binali, Satya Sudheer Pydi, William Goodwin Source Type: research

Developing legal regulation of forensic dna-phenotyping in hungary
Traditional DNA forensics uses short tandem repeats (STRs) to match a DNA sample to a suspect or a database. However, when no suspect has been identified and there is a lack of database hits, these markers cannot help the investigators to solve a crime. A considerable amount of literature has been published on DNA phenotyping in the past few years. Not surprisingly, some ethical and legal aspects are needed to be discussed about FDP since this technology provides a greater number of details about the proprietary ownership of samples, their family members, even as about their larger community (ethnical group). (Source: Fore...
Source: Forensic Science International: Genetics Supplement Series - October 22, 2019 Category: Forensic Medicine Authors: Nogel M ónika, Pádár Zsolt, Czebe András, Kovács Gábor Source Type: research

Novel index of body fluid-rna integrity based on small rna electropherogram
Ensuring RNA integrity is important in RNA experiments because RNA molecules are highly labile. We previously reported that RNA integrity number (RIN) was not accurate for examining RNA integrity in body fluid. In the present study, we developed a new index for body fluid-RNA integrity using the small RNA electropherogram. We first compared the small RNA electropherogram with the total RNA electropherogram in venous blood RNA. Then, to develop the RNA integrity index, we analyzed the small RNA electropherogram by partial least squares regression. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 22, 2019 Category: Forensic Medicine Authors: Shuntaro Fujimoto, Sho Manabe, Eriko Hirai, Chie Morimoto, Keiji Tamaki Source Type: research

Background DNA on flooring: The effect of cleaning
The collection and detection of ‘background’ DNA (bDNA) from items submitted for forensic DNA sampling is a common occurrence when sampling surfaces for DNA left behind following an activity of interest. There are a number of factors that might affect the presence of bDNA on a surface, one of which is cleaning of the surface. Here, we investigate the impact of household cleaning methods on the presence of bDNA on flooring in five homes occupied by known and unrelated individuals. Two adjacent samples were collected pre- and post-cleaning from areas of high and low activity on floors within the kitchen, living room, bed...
Source: Forensic Science International: Genetics Supplement Series - October 22, 2019 Category: Forensic Medicine Authors: J.B. Reither, E. Gray, A. Durdle, X.A. Conlan, R.A.H. van Oorschot, B. Szkuta Source Type: research

Effect of phi29 polymerase-based multiple strand displacement whole genome amplification on the proportion in DNA mixtures
Multiple displacement amplification (MDA), an isothermal whole genome amplification method, has been applied extensively for its long amplification products and low bias. Because of preferential amplification and allelic drop-out with MDA when dealing with low template DNA ( (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 22, 2019 Category: Forensic Medicine Authors: Qiang Zhu, Ting Fang, Yijun Zhou, Yiwen Yang, Yueyan Cao, Qiuyue Wang, Yuguo Huang, Yuhan Hu, Xiaogang Chen, Yufang Wang, Ji Zhang Source Type: research