Forensic Science International: Genetics Supplement Series This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Population genetic data for 17 non-CODIS STR loci for the Saudi Arabian population using the SureID ®23comp Human Identification Kit
Our previous work focused on validation the SureID 23comp Human Identification Kit (Health Gene Technologies, China), following the minimum criteria for validation recommended by the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) using 500 samples from the population of Saudi Arabia. The kit genotypes 22 STRs, 17 of which are non-CODIS, and Amelogenin. The validation tests showed that it has the potential to increase the power of testing in complex cases. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: Hussain M Alsafiah, William H Goodwin Source Type: research

A multipurpose panel of microhaplotypes for casework
Microhaplotypes (MHs) are SNP-based multiallelic loci that have several advantages over individual SNPs and short tandem repeats (STRs). For several years we have been searching for better MHs based on the effective number of alleles at a locus (Ae) and the locus informativeness for population relationships (In) with thoughts of incorporating MHs into casework. We genotyped a multiplex of our best 90 MHs on 79 populations. We have ranked the 90 by Ae and analyzed the top 24 to evaluate their potential value in forensic casework. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: Kenneth K. Kidd, Andrew J. Pakstis, Neeru Gandotra, Curt Scharfe, Daniele Podini Source Type: research

Development of a nanoplate-based digital PCR assay for species identification with mixture deconvolution
In crime scenes, not all biological stains are human in origin. Some exhibits can be from pets living on the premises or from animal products used in food consumption. In addition, it could be necessary to test animal carcasses for other forensic purposes. Often such stains can include mixtures involving humans or other species. Thus, identifying and deconvoluting mixtures of species commonly found in and around a household can be crucial in forensic casework. Different molecular techniques have been employed for species identification such as immunoprecipitation, qPCR, and DNA sequencing. (Source: Forensic Science Interna...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: Mirna Ghemrawi, Bruce McCord Source Type: research

Probabilistic SNP Genotyping at Low DNA Concentrations
We present a statistical method for biallelic SNP genotyping that reduces the risk of wrong SNP calls and gives fewer no-calls. The method uses a symmetric multinomial logistic regression model with an intuitive graphical interpretation. Its probabilistic nature gives the user control over the accepted risk through the estimated genotype probabilities. We compared the performance of our method with the HID SNP Genotyper v.4.3.1 plug-in (HSG) (Thermo Fisher Scientific) and the additional criteria of the University of Copenhagen (UCPH) through a series of six DNA dilutions from 500 pg to 16 pg DNA. (Source: Forensic Science ...
Source: Forensic Science International: Genetics Supplement Series - October 13, 2022 Category: Forensic Medicine Authors: M.B. Nielsen, M.M. Andersen, P.S. Eriksen, H.S. Mogensen, N. Morling Source Type: research

Recovery of DNA from bone without demineralization
This study assessed the performance of five different DNA extraction methods for the recovery of DNA from bone: ChargeSwitch ® gDNA Plant Kit, DNA IQTM System Kit, DNeasy® Blood& Tissue Kit, PrepFiler ® BTA Forensic DNA Extraction Kit and phenol-chloroform-isoamyl alcohol. DNA was extracted from pig rib and femur bones that was fresh, had undergone surface decomposition for three months, and had undergone surface decomposition for one year. Extracted DNA was analyzed using real-time PCR and ampl ification of an in-house PCR multiplex that assessed the quality and quantity of DNA and for the presence of inhibitors. (Sour...
Source: Forensic Science International: Genetics Supplement Series - October 13, 2022 Category: Forensic Medicine Authors: Sasitaran Iyavoo, William H. Goodwin Source Type: research

Test for contamination in connection with renovation of post-PCR laboratories
The Teilum building housing the Department of Forensic Medicine at the University of Copenhagen was renovated in 2021/22. All windows were replaced, and the heating system was upgraded. During the renovation, the usual measures to prevent PCR products from escaping the post-PCR laboratories could not be maintained, since construction workers had to move in and out of the rooms carrying tools and debris. Instead, new measures were introduced, that included 1) the construction of a changing room for the workers with immediate access to the post-PCR laboratories, 2) clothing and shoes for the workers, that should only be worn...
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Cathrine B. Petersen, Christina M. Dahmcke, Anders Buchard, Claus B ørsting Source Type: research

Development and validation of molecular methodologies for identification of biological traces from crime scenes
This study aimed to develop a methodology to identify biological fluids in sexual assault cases through mRNA markers. Biological fluid samples such as blood, saliva, and semen were collected from volunteers and submitted to RT-qPCR reactions with specific primers for the biomarkers HTN3 (saliva), ALAS (blood) andTGM4 (semen). The Melting (Tm) of each biological fluid was analyzed and the result inferred a high specificity capable of differentiating such traces. Biplex systems were generated to improve trace analysis in a single qPCR reaction. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Alessandra S. Dias, Anna Beatriz Rodrigues Gon çalves, Tatiana Lúcia Santos Nogueira, Dayse A. Silva Source Type: research

An ambiguous sequence-based allele of SE33
SE33 was a well-known autosomal short tandem repeat (STR) marker that was high polymorphic and therefore was high discrimination power. The sequence structure of STR markers has been increasingly explored with next-generation sequencing (NGS) technology. The sequencing resulted in the development of a new locus designation and allele nomenclature that was also backward compatible with the conventional capillary electrophoresis. SE33 was one of the STR markers that had been coamplified by Forenseq ™ Signature Prep Kit (Verogen) but were not analyzed and illustrated in the Universal Analysis Software (UAS) (Verogen). (Sour...
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Tikumphorn Sathirapatya, Hasnee Noh, Poonyapat Sukawutthiya, Wikanda Worrapitirungsi, Kornkiat Vongpaisarnsin Source Type: research

Analysis of an ancestry using cremated old human remains from the Korean War victims
In this study, we pursue the best technical approach for the minimal damage and the contamination of DNA from other factors in the preconditioning and the extraction process based on over 70 years old Korean War victim skeletal that was burnt and buried in Korean Peninsula. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Yu Na Oh, Jung-Hyun Park Source Type: research

How to regulate forensic familial DNA searching in Hungary?
Forensic DNA analysis has the potential to provide useful information for criminal justice even in cases where there is no match, neither between the DNA profile generated from the crime scene and the existing DNA profiles in criminal databases, nor between the DNA collected at a crime scene and potential suspects. In contrast to traditional forensic genetic testing, forensic familial DNA searching does not provide evidence, but helps to generate investigative leads and narrow down the range of potential offenders. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: M ónika Nogel Source Type: research

Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories
Sodium hypochlorite is an efficient reagent for removal of unwanted DNA from laboratory surfaces. Here, we tested two different chlorine wipes and compared their performance to a 0.9-1.8% hypochlorite solution. WipeClean Chlorine Disinfection wipes contain>0.1g sodium hypochlorite/kg, whereas WetWipe Chlorine Desinfection wipes contain>1000 ppm active chlorine. Clean surfaces were contaminated with 10 µl 0.5ng/µl of massively parallel sequencing libraries. The DNA was dried and left for 45minutes before any treatment. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Marie-Louise Kampmann, Bo Thisted Simonsen, Claus B ørsting Source Type: research

Is there human DNA on cats
The transfer, persistence, prevalence, and recovery of DNA (DNA-TPPR) can be highly relevant in forensic investigations to evaluate the presence and/or actions of a person of interest (POI). Whilst the DNA-TPPR-related research has increased significantly over the last decade, there is a lack of data on companion animals and their relationship to human DNA transfer. Given the commonality of cats and dogs in households around the world, companion animals as receptors and vectors for DNA transfer can be highly relevant in cases involving animals as victims of a criminal offense, or cases requiring activity level evaluations....
Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Heidi Monkman, Roland A.H. Van Oorschot, Mariya Goray Source Type: research

Evaluation of genetic markers for the analysis of THC levels of Cannabis sativa samples using principal component analysis – A preliminary study
In this study, principal component analysis (PCA), an exploratory data analysis technique, was tested to correlate the specific genotype with the concentration of tetrahydrocannabinol (THC) in the samples. C. sativa samples were obtained from legal growers in Piedmont, Italy, and from illegal drug seizures in the Turin region. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 6, 2022 Category: Forensic Medicine Authors: S. Cisana, M. Omedei, M. Di Nunzio, F. Seganti, V. Brenzini, A. Coppi, A. Berti, C. Di Nunzio, P. Garofano, E. Alladio Source Type: research

Comparisons of protocols for enhanced DNA profile quality from FTA ®-deposited urine samples
Disputes over the identity of a urine sample donor have been reported, and urine authentication by genetic profiling has helped resolved the cases. However, since genotyping of urine is not always required, many drug-testing laboratories may face sample storage issues. Several studies have investigated the use of FTA ® cards as a convenient tool for keeping specimen at room temperature for extended periods of time. However, generating complete STR profile from some FTA®-deposited urine samples remains challenging due to low levels of genetic material content, necessitating amendments to the laboratory’s stan dard proto...
Source: Forensic Science International: Genetics Supplement Series - October 6, 2022 Category: Forensic Medicine Authors: Korapin Srisiri, Tikumphorn Sathirapatya, Achirapa Bandhaya Source Type: research

Paternal half-sisters or unrelated – How can the X-chromosomal evidence be quantified when one female presents trisomy X?
The X chromosome has unique features, such as haplodiploid mode of genetic transmission, which can be crucial to complement autosomal profiling or to disentangle complex kinship problems. Indeed, for some cases (e.g. full- or paternal half-sisters, or paternal grandmother-granddaughter hypotheses), X-markers are expected to provide a similar or a higher power to the one obtained with autosomes in paternity/maternity investigations. Both theoretical and informatics frameworks for pairwise X-linked kinship analyses are well established for individuals with a regular number of chromosomes, but these are still lacking for indi...
Source: Forensic Science International: Genetics Supplement Series - October 6, 2022 Category: Forensic Medicine Authors: Marisa Faustino, Chiara Turchi, Daniel Kling, Leonor Gusm ão, António Amorim, Nádia Pinto Source Type: research