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A Study of Genetic Analysis using Novel Rapidly Mutating Y-STR Multiplex for Qatari population
Differentiating male lineages using non-recombining Y-chromosomal genetic markers is highly informative for tracing human migration and for forensic studies. The aim of this study was to develop allelic frequency to evaluate the resolution power of Rapidly Mutating Y-STR (RMY-STR) on Qatar population in comparison to Worldwide population. In the Qatar population the haplotype resolution of 100% was achieved. The new set of RMY-STR markers showed remarkable haplotype resolution power in the Qatar population, high gene diversity and sufficient robustness. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 13, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research

Forensic Evaluation of Verifiler ™ Plus 6-Dye Chemistry Kit Composed of 23 Loci With Casework Samples
In this study, I have evaluated a six-dye STR multiplex assay composed of 23 autosomal STR loci, 1 insertion/deletion polymorphic marker on the Y chromosome, Amelogenin and two internal quality control markers (IQCS and IQCL). This Internal validation indicated that VeriFilerTM Plus PCR amplification kit was reproducible, sensitive, accurate and robust. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 13, 2019 Category: Forensic Medicine Authors: Naeema Al Janaahi, Rashed Al Ghafri, Synan Abu Qamar Source Type: research

CYT B L15601 and H15748 primers for genetic identification of cetacean species
Dolphins and other cetacean are protected species whose capture is illegal in many countries. Despite the high mercury content accumulated by these animals, occasional episodes of illegal fishing occur to supply certain black markets with dolphin meat. When fishing authority inspections detect meat portions presumably of cetacean origin, it is particularly necessary to identify the species before taking the case to court. The mitochondrial cytochrome b gene has been used extensively to identify species. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 11, 2019 Category: Forensic Medicine Authors: A.N.D.R. É.S. Lopez-Oceja, X.A.B.I.E.R. Lekube, L.E.I.R.E. Ruiz, J.O.S.E.A.N.T.O.N.I.O. Mujika-Alustiza, M.A.R.I.A.N.M. De Pancorbo Source Type: research

The study of novel sequence alleles for qatari population using forenseqtm dna kit
For the last three decades, Short Tandem Repeat (STR) markers and capillary electrophoresis-based DNA sequencers have been the gold standard technology for human identification testing in the forensic field. MPS technology has enabled sequencing of several types of genetic loci in one multiplex including single nucleotide polymorphisms (SNPs) and STRs. One hundred and fifty (150) reference samples were profiled using the ForenSeqTM DNA Signature kit. PCR Primer Mix B for this kit was used containing autosomal/Y/X STRs and AISNPS, iSNPs and pSNPs loci. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 11, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research

The Study of 95 Identity SNPs for Qatari population using Massively Parallel Sequencing (MPS)
For the last three decades, Short Tandem Repeat (STR) markers and capillary electrophoresis-based DNA sequencers have been the gold standard technology for human identification testing in the forensic field. However, Massively Parallel Sequencing (MPS) has the potential to replace the current CE-based technology. All laboratories globally, including the Qatar Forensic Laboratory, have stringent strategies and regulations in improving and processing high numbers of reference and casework samples, using instrumentation and technologies to maximize output using new STR kits consisting of larger number of loci. (Source: Forens...
Source: Forensic Science International: Genetics Supplement Series - November 10, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research

Genetic polymorphisms of 30 insertion/deletion markers in the populations of Japan, Bangladesh, and Indonesia
Population studies of 30 insertion/deletion (INDEL) markers were conducted using the Investigator ® DIPplex kit (Qiagen) in 197, 80, and 78 unrelated individuals from Japan, Bangladesh, and Indonesia, respectively. No significant deviations from Hardy–Weinberg equilibrium were observed for all but three INDEL markers: two markers in the Japanese samples and one marker in the Bangladeshi and I ndonesian samples. The combined power of discrimination values for the 30 INDEL markers in the Japanese, Bangladeshi, and Indonesian populations were 0.999999999983, 0.999999999999, and 0.999999999937, respectively. (Source: Forens...
Source: Forensic Science International: Genetics Supplement Series - November 8, 2019 Category: Forensic Medicine Authors: Atsushi Nagai, Toshimichi Yamamoto, Masaaki Hara, Tomomi Michiue Source Type: research

Comparison of minidogfiler and "asch" str multiplex systems for preliminary estimation of variability within wolf ´s like dog breeds
This study was undertaken to estimate basic population parameters and compare resolution capabilities of two chosen STR panels (Mini-DogFiler, MDF; “ASCH”). Wolf´s like dog breeds, namely Czechoslovakian wolfdog (n = 55), Saarloos wolfdog (n = 22), and samples of the Eurasian wolf (n = 21) were selected for their close genetic relationship. Also, German Shepherd (n = 26) breed was included as a donor of dog genome for the C zechoslovakian wolfdog.Overall 228 alleles were detected using 20 STR markers (MDF 11 and “ASCH” 9 loci). (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 8, 2019 Category: Forensic Medicine Authors: Radka Štikarová, Jakub Vašek, Eniko Kubinyi, Daniela Čílová, Pavel Vejl Source Type: research

Proceedings of the 28th ISFG Congress
The 28th Congress of the International Society for Forensic Genetics (ISFG) took place in Prague, the capital of the Czech Republic. It was the first ISFG Congress held in the region of the previous Czechoslovakia and experienced a record number of attendees. Stick-on footsteps led 1018 participants from the Vy šehrad metro station to the Prague Congress Centre. These proceedings, which have been published in Forensic Science International: Genetics Supplement Series Volume 7, contain more than 330 articles describing work presented at the conference – another ISFG record! (Source: Forensic Science International: Geneti...
Source: Forensic Science International: Genetics Supplement Series - November 7, 2019 Category: Forensic Medicine Authors: Ji ří Drábek, Mechthild Prinz, John M. Butler Tags: Editorial Source Type: research

DIACHRONIC mtDNA STUDY OF THE LONG TIME OCCUPIED ARCHAEOLOGICAL SITE OF SEGOBRIGA (SPAIN) AND COMPARISON WITH NOWADAYS POPULATION
This study is focused on 14 archaeological individuals from the Segobriga archaeological site (Sa élices, Cuenca, central-eastern Spain). The relevance of Segobriga is that it has been occupied from the V century B.C. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 6, 2019 Category: Forensic Medicine Authors: S. Palomo-D íez, R. Cebrián-Fernández, I. Hortelano-Uceda, E. Labajo-González, B. Perea-Pérez, C. Gomes, E. Arroyo-Pardo Source Type: research

Detection of forensic identification and intelligence snp data from latent dna using three commercial mps panels
Massively parallel sequencing (MPS) offers the ability to detect and analyse low quantity and quality DNA present on touched items. Here, we present MPS identity and intelligence SNP data generated from latent DNA using direct PCR and three different forensic panels: (1) the QIAGEN 140-SNP forensic identification multiplex (2) the 24 SNP HIrisplex System and (3) the 165 SNP Precision ID Ancestry Panel. The effect of individual and substrate type (wire, glass and zip-lock bag) on the recovery of SNPs and prediction accuracy was examined and compared across the three panels. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 6, 2019 Category: Forensic Medicine Authors: J.M. Young, B Martin, P. Kanokwongnuwut, A Linacre Source Type: research

Assessing dna recovery from highly degraded skeletal remains by using silica-based extraction methods
Through this study, we extracted human DNA as for five different type of skeletal remains: petrous bone, tooth pulp cavity, tooth cementum, rib and at least two different upper limbs bones (radial, ulna, metacarpal or phalange) from five individuals (5-12th centuries AD) by using four extraction methods; three based on DNA adsorption to silicon dioxide (silica) particles, Non-Columns Silica (NCSi), Silica-HE Spin Columns (SiHEC), Silica-XS Spin Columns (SiXSC) and the traditional organic extraction method (P-Chl) (N:100 DNA extracts). (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 6, 2019 Category: Forensic Medicine Authors: Diana C. Vinueza-Espinosa, Cristina Santos, Cristina Mart ínez-Labarga, Assumpció Malgosa Source Type: research

Evaluation of HIrisplex-S System markers for eye, skin and hair color prediction in an admixed Brazilian population
In this study, we present the first evaluation of the HIrisPlex-S system in an admixed population. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 4, 2019 Category: Forensic Medicine Authors: Leonardo A. Marano, Jeppe D. Andersen, Fernanda T. Goncalves, Ana Laura O. Garcia, Cintia Fridman Source Type: research

Mitochondrial genetic profile of the yoruba population from nigeria
Nigeria is located in the Gulf of Guinea, also known as the “giant of Africa”, being the seventh most populated country in the world. The territory has a high genetic and cultural diversity. Yoruba is the second major ethnic group (and the most spoken language), and its population is concentrated in the southern region of the country. Aiming the elaborat ion of an accurate forensic mitochondrial DNA database for this region, the mtDNA control region was analysed from 71 Yoruba individuals. 65 unique haplotypes was found resulting in a haplotype diversity of 0.9976 ± 0.0028. (Source: Forensic Science International: Gen...
Source: Forensic Science International: Genetics Supplement Series - November 4, 2019 Category: Forensic Medicine Authors: B. Mart ínez, M. Nguidi, L. Catelli, C. Vullo, V.O. Okolie, S.O. Keshinro, E.F. Carvalho, L. Gusmão, F. Simão Source Type: research

How many STR markers are enough?
The knowledge of relatedness between individuals is central to many studies in population ´s genetics. To obtain estimates of relatedness with sufficient accuracy, the number of polymorphic markers and the number of alleles per marker is a crucial factor. Because of their allelic diversity, microsatellites, also known as short tandem repeats (STRs), are often recognized as the most effi cient genetic markers (Lynch and Ritland 1999). The aim of our study was to examine the ability of 15 and 22 STR markers included in the Powerplex16 and Powerplex Fusion Kits to detect biological kinship, even in presence of presumptive mu...
Source: Forensic Science International: Genetics Supplement Series - November 1, 2019 Category: Forensic Medicine Authors: Antonella Belen Penacino Source Type: research

Detritus: an approach to extracting useful information from complex mixtures that include human dna
This is a study of quality and utility of the products of arbitrarily primed PCR amplifications. The main finding is that the fidelity of arbitrarily primed amplifications of the type we have used for soils is good and the sequences are satisfactory for species identification, at least for the human genome and, by inference, for other genomes. This type of amplification of DNA samples from human tissues results in a population of sequences from locations distributed across the human genome. Base composition and CG/GC ratio in the amplimers differs from that of the whole genome to a degree similar to that of subsets of sequ...
Source: Forensic Science International: Genetics Supplement Series - November 1, 2019 Category: Forensic Medicine Authors: Burgoyne Leigh Source Type: research