Forensic Science International: Genetics Supplement Series This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

High-performance LAMP-based method for human sex identification using Y chromosome-specific genetic markers
We report a new versatile alternative for molecular sex determination using Y-specific targets (TSPY, TTTY, alphoid regions, and Y-Amelogenin). This system uses an isothermal loop-mediated DNA amplification (LAMP) with a set of 6 primers for each target, designed to improve sensibility and specificity, and reducing detection time to only 45  min. Furthermore, detecting the different targets on the Y chromosome either individually or in combination revealed accurate results. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Germ án Burgos, Katherin Barrionuevo-Pérez, Tomás Restrepo, Eduardo Tejera, Jacobus H. de Waard, Alejandra Garzón-Salazar, Leonor Gusmão Source Type: research

Using previous DNA casework data to aid decision making in the process of DNA profile interpretation
In this study, a DNA decision support tool was developed. With this tool we aim to gain insight in what actions are performed with which (types of) DNA profiles in casework, to improve decision making by DNA experts in future cases. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Corina C.G. Benschop, Charissa van Kooten, Dion Zandstra, Pieterjan Sjoukema, Francisca E. Duijs, Margreet van den Berge, Colin Schepers, Jeroen Mandersloot, Rolf Ypma Source Type: research

Genetic variation of different Peruvian populations using 23 autosomal STR markers
In the present study the genetic variation of different Peruvian populations was investigated. The samples for this study were obtained from 669 individuals distributed among 11 populations from Peru. All samples were analyzed using 23 autosomal STR markers. The Arlequin v3.5.2.2 software was used to determine the genetic distances (Fst) of the studied populations. Notable population substructure was detected between some populations. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: C.D. Neyra-Rivera, B. Budowle Source Type: research

An experimental extension to the discrete Laplace method for Y-STR haplotype frequency estimation
Frequency estimation for Y-STR haplotypes is a challenging problem because limited data are available and complex dependencies exist within the data. As a result, various statistical methods have been proposed for frequency estimation. The discrete Laplace method has been recommended in some contexts by the DNA commission of the ISFG. This method is limited to haplotypes with single integer repeat alleles only at all loci. We propose a generalisation of the method that handles duplicated loci such as DYS385 and less common alleles that are not integer repeats. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Maarten Kruijver, Duncan Taylor, John Buckleton Source Type: research

Genetic Analysis Instrumentation Innovations Built into the SeqStudio â„¢ Flex Genetic Analyzers
The new Applied Biosystems â„¢ SeqStudio â„¢ Flex Series Genetic Analyzer have improved the benchmark for research use only for Capillary Electrophoresis (CE) by providing innovative approaches to enhanced hand-free operation, flexibility, ease of use, data quality and connectivity. This newly designed 8 or 24 capillary syste m supports fragment sizing and DNA sequencing applications providing scientists with medium throughput technology for use in research applications. The steps from system set-up to size or base-called data have been simplified with hardware functionality and user-friendly software enhancements design e...
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Pranapda Katie Aumsuwan, Steve Berosik, Patricia Chege, Adam Sannicandro, Siva Samsani, John Bodeau, Greg Foster, David Rodriguez, Erin Lagier, Stephanie Chee, Scott Nelson, Carole Bornarth Source Type: research

Pitfalls, challenges and caveats in whole mitochondrial genome sequencing from hair shafts by MPS: where, when and how to address them
The growing use of massively parallel sequencing (MPS) for the whole mitochondrial genome analysis in forensic laboratories, requires the establishment of efficient work flows and interpretation procedures, to support the feasibility of the technology and the reliability of the data. In the case of reference samples, such as blood and buccal swabs, the generation of mtDNA profiles by using MPS is relatively simple. Conversely, many forensic casework samples still p ose challenges for the MPS, data interpretation and reporting of mtDNA. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Chiara Turchi, Filomena Melchionda, Federica Alessandrini, Valerio Onofri, Mauro Pesaresi, Loredana Buscemi, Adriano Tagliabracci Source Type: research

Efficiency evaluation of the LT-DNA traces analysis modifications
Analysis of genetic profiles obtained from low template DNA samples (LT DNA) can be challenging because of increased probability of stochastic amplification artifacts occurrence. According to the recommendations of international genetic societies the quality of the LT-DNA traces results can be improved by applying low copy number (LCN) methods. Another strategy which allows to obtain better results of the analysis of LT-DNA traces is replicate the amplification of the same DNA sample and create consensus, composite (virtual pool profile) or real pool profile. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Agnieszka Parys-Proszek, Magdalena Marci ńska, Maria Wróbel Source Type: research

Automated DNA casework workflow: A retrospective study of the first implementation of FIDL at the Netherlands Forensic Institute
FIDL is a fast and automated DNA identification line which represents a series of software solutions automating the process from raw capillary electrophoresis data to reporting. This retrospective study provides insight in the numbers of cases, turnaround time, results compared to the standard workflow and the benefits automation has in a large volume workflow. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Corina C.G. Benschop, Martin Slagter, Sophie Smit, Alexander L.J. Kneppers Source Type: research

STRSeq: FAQ for submitting
The STR sequencing project was developed due to the necessity of publicly sharing sequencing information about Short Tandem Repeats (STR) associated with human identification. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) is becoming more prevalent in forensics. Having a publicly accessible centralized location to maintain this forensic-specific type of sequencing information is useful for the community. It is also important that the community participate in providing information to strengthen this resource. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Lisa A. Borsuk, Peter M. Vallone, Katherine B. Gettings Source Type: research

The killer outfit and timing: impact of the fabric and time in body fluid identification and DNA profiling
The present work aimed to study the detection, through lateral flow immunochromatographic (LFI) tests, of saliva samples over time in three different types of fabrics, as well as, the possibility of DNA isolation and characterization from the sample tubes and the cassettes. Fifty microliters of saliva (three samples/time) were deposited in denim, cotton, and polyester. Saliva was identified by SERATEC Amylase Test and the Crime Scene version SALIVA CS, being able to detect it up to six months of deposition, although with different band intensities. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Sara C. Zapico, Valerie Lascano, Tarik Sadik, Proggya Paromita, Jenely Amaya, Christian Stadler, Gabriela Roca Source Type: research

Empirical haplotype calling and probabilistic interpretation of microhaplotype profiles
MicroHapulator ’s empirical microhaplotype calling algorithm produces profiles well-suited for forensic analyst interpretation and probabilistic interpretation. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Daniel Standage, Rebecca Just, Curt Scharfe, Neeru Gandotra Source Type: research

Comparison of Metal Ions Recovered During DNA Analysis of Brass Ammunition and Effects of Copper and Zinc Ions on DNA Profiling
Analyzing DNA from brass surfaces poses unique challenges that may result from DNA damage and/or PCR inhibition. To examine the relationship between the metal ions present in brass ammunition and the success of Short Tandem Repeat (STR) profiling, six recovery methods were tested to determine the identity and quantity of metal ions co-recovered during DNA sampling. In addition, DNA and metal ion solutions were created at varying concentrations to determine the threshold at which deleterious effects occur. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Natalia Czado, Rachel Houston, Sheree Hughes Source Type: research

High-performance LAMP based method for human sex identification using Y chromosome specific genetic markers
We report a new versatile alternative for molecular sex determination using Y-specific targets (TSPY, TTTY, alphoid regions, and Y-Amelogenin). This system uses an isothermal loop-mediated DNA amplification (LAMP) with a set of 6 primers for each target, designed to improve sensibility and specificity, and reducing detection time to only 45minutes. Furthermore, detecting the different targets on the Y chromosome either individually or in combination revealed accurate results. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2022 Category: Forensic Medicine Authors: Germ án Burgos, Katherin Barrionuevo-Pérez, Tomás Restrepo, Eduardo Tejera, Jacobus H. de Waard, Alejandra Garzón-Salazar, Leonor Gusmão Source Type: research

Predicting the optimal STR profile amplification set up from Quantifiler â„¢ Trio data
Samples collected for forensic case work may be of varying quality and quantity. The sample DNA is often quantified prior to short tandem repeats (STR) profile analysis with methods such as Quantifiler â„¢Trio (QFT). The QFT measures the quantity of DNA as well as an internal PCR control (IPC) and a degradation index (DI).The aim of this study was to use IPC and DI measurements to identify samples, which would benefit from a modified PCR amplification set-up when generating the STR profiles. The s ample quality of 6287 single source case work samples were categorized as 'Good’, ‘Partly degraded’, ‘Highly degradedâ€...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: Mikkel E. Dupont, Marie-Louise Kampmann, Ditte M. Truelsen, Cathrine B. Petersen, Jeppe D. Andersen Source Type: research

DNA extraction of burnt bone and teeth casework samples using bead-beating homogenization technique
Sample disruption was a necessary step for DNA isolation. Bone and teeth were useful biological sources particular in human remains and advance decomposed bodies. The compact bone and teeth required several preparation steps prior to analyzing process. However, the methods in standard protocol were laborious and time consuming. An alternating pulverization, bead beating homogenizer, was purposed in its effectiveness for forensic casework. (1) Here, we applied this technique to the burnt cracked bone and tooth that recovered from house fire for forensic DNA analysis. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: P. Varrathyarom, T. Sathirapatya, W. Worrapitirungsi, K. Vongpaisarnsin Source Type: research