Forensic Science International: Genetics Supplement Series This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Differential extraction method as a golden standard in analyzing semen stains in sexual-assault cases
In the investigation of sexual-assault cases different analysis of collected biological samples from the victim and from the crime scene are applicable, depending on the results of the presumptive tests for sample characterization. DNA testing of semen stains can be performed in different ways. Differential extraction method is a golden standard for analysis of sexual assault mixture samples of sperm cells and victim epithelial cells, allowing straight forward interpretation of perpetrator ’s DNA profile. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 21, 2019 Category: Forensic Medicine Authors: Renata Jankova, Zlatko Jakovski, Robert Janevski Source Type: research

Heterozygous 21 STR Loci and Triplet Alleles Observed in Population Genetic Analysis of the GlobalFiler STR loci in the Ghanaian population
This study profiled 1,047 volunteers using the GlobalFiler â„¢ Kit from the four main ethnic groups in Ghana: the Akan (282), Mole-Dagomba and Northern ethnic minority (253), Ewe (250) and Ga-Dangbe (262), representing about 98.4% ethnic coverage of the Ghanaian population.The polymorphic nature of the African population was demonstrated during the profilin g of the reference data set with the occurrence of some rare triplet variant alleles. At the TPOX locus, three tri-allelic were found with the allelic patterns 6-8-10, 8-9-10 and 9-10-11. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 21, 2019 Category: Forensic Medicine Authors: Pet-Paul Wepeba, Arati Iyengar, William Goodwin Source Type: research

An unusual kinship case from the Spanish Civil War (1936 –1939): Ancient versus degraded sample’s investigation
An unusual kinship case arrived to our laboratory, having as main objective to perform a genetic analysis between a living presumable niece and some human remains –eight bones from a hand. The bone samples probably belonged to a cleric executed in Almeria (Spain), during the Spanish Civil War (1936–1939), where, after the execution, the cadaver was thrown to a dry well and covered with lime. Later, the cadaver was transferred to a local cemetery, where du e to a flood, was then relocated in another sanctuary. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 21, 2019 Category: Forensic Medicine Authors: C. Gomes, M. Fondevila, C. Maga ña-Loarte, J. Fernández-Jiménez, J. Fernández-Serrano, S. Palomo-Díez, C. Baeza-Richer, A.M. López-Parra, E. Arroyo-Pardo Source Type: research

Underestimation and misclassification of mutations at X chromosome STRs depend on population ’s allelic profile
Short tandem repeats are extremely abundant in the human genome and widely used in population and forensic genetics. The conventional approach to estimate mutation rates for STRs involves the detection of Mendelian incompatibilities and their frequency quantification, in family duos or trios. In this work, we aim to measure the impact this estimation methodology implies in the underestimation of mutation rates.We computed two independent experiences simulating one single-step mutation and one double step mutation in each pedigree. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 21, 2019 Category: Forensic Medicine Authors: S. Ant ão-Sousa, E. Conde-Sousa, L. Gusmão, A. Amorim, N. Pinto Source Type: research

The impact of FDSTools noise correction on the analysis of data from the Forenseq ™ DNA Signature Prep Kit
Massively Parallel Sequencing (MPS) enables simultaneous analysis of a much larger number of forensic markers compared to conventional capillary electrophoresis (CE) sizing-based genotyping. Since both methods rely on amplified STR fragments, stutter products will occur that can complicate the detection of minor contributors in mixed samples. Sequence variation observed in STRs and their flanking regions can help to distinguish genuine alleles from stutter artefacts and noise correction can be applied to filter stutter artefacts. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 21, 2019 Category: Forensic Medicine Authors: K.J. van der Gaag, J. Hoogenboom, L. Busscher, C.C.G. Benschop, S. Zu ñiga, T. Sijen Source Type: research

An unusual kinship case from the Spanish Civil War (1936 –1939): Ancient versus degraded sample’s investigation
An unusual kinship case arrived to our laboratory, having as main objective to perform a genetic analysis between a living presumable niece and some human remains –eight bones from a hand. The bone samples probably belonged to a cleric executed in Almeria (Spain), during the Spanish Civil War (1936–1939), where, after the execution, the cadaver was thrown to a dry well and covered with lime. Later, the cadaver was transferred to a local cemetery, where du e to a flood, was then relocated in another sanctuary. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: C. Gomes, M. Fondevila, C. Maga ña-Loarte, J. Fernández-Jiménez, J. Fernández-Serrano, S. Palomo-Díez, C. Baeza-Richer, A.M. López-Parra, E. Arroyo-Pardo Source Type: research

An unusual kinship case from the Spanish Civil War (1936-1939): Ancient versus Degraded sample's investigation
An unusual kinship case arrived to our laboratory, having as main objective to perform a genetic analysis between a living presumable niece and some human remains –eight bones from a hand. The bone samples probably belonged to a cleric executed in Almeria (Spain), during the Spanish Civil War (1936-1939), where, after the execution, the cadaver was thrown to a dry well and covered with lime. Later, the cadaver was transferred to a local cemetery, where due to a flood, was then relocated in another sanctuary. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: C. Gomes, M. Fondevila, C. Maga ña-Loarte, J. Fernández-Jiménez, J. Fernández-Serrano, S. Palomo-Díez, C. Baeza-Richer, A.M. López-Parra, E. Arroyo-Pardo Source Type: research

The presence of background DNA on common entry points to homes
This study investigates the presence of such DNA, also termed background DNA (bDNA), on common entry points in five homes occupied by known individuals. Samples were collected from internal and external windowsills and edges within bedrooms and living rooms, as well as front and rear door handles. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: B Szkuta, JB Reither, XA Conlan, RAH van Oorschot Source Type: research

Forensic dna registration in the russian federation: background and the current state
The report addresses the background and the current state of forensic DNA registration (state genomic registration) in Russia. Some legal and forensic aspects concerning the forensic DNA database (Federal database of genome information - FDBGI) with regards to both the investigation aims and the guarantee of the civil rights are discussed. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: I.O. Perepechina Source Type: research

Analysis of paternal lineages legacy in salta province, northwestern argentina
Seventeen Y-STRs included in the AmpFlSTR ® Yfiler® PCR Amplification Kit were studied for 174 individuals from Salta province (northwest of Argentina). A total of 166 different haplotypes were identified. The Haplotype Diversity (HD) was 0.9992 and the Discrimination capacity (DC) was 95.4%. The high efficiency in forensic parameters dem onstrates that this set of markers provides a powerful tool in forensic casework in Salta population. Haplogroup composition reflects the Amerindian and European legacy of this population together with a minor African contribution. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: J.F. Ferragut, M.V. Albeza, N. Acreche, J.A. Castro, C. Ramon, A. Picornell Source Type: research

Admixture and population structure in mayas and ladinos from guatemala based on 15 strs
Guatemala is a Central American country with a large Native-American ancestry whose genetic structure has been poorly studied. Consequently, we created and compilated Guatemalan population-databases for 15 autosomal STRs previously reported. Population sample included 581 unrelated Mayan volunteers from nine indigenous groups, in addition to 130 unrelated Ladinos. Structure analysis showed low differentiation among Ladino and Mayan populations from Guatemala (Fst  = 0.74%), which did not increase importantly when Ladino were excluded (Fst = 0.78%). (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Jos éAlonso Aguilar-Velázquez, Mishel Sthepenson-Ojea, Marco Antonio García-King, H.éctor Rangel-Villalobos Source Type: research

Investigation on Rapidly Mutating Y-STRs multiplex in Indian population: A pilot study
Importance of rapidly mutating (RM) Y- STRs) is already established and is evident with the addition of seven RM Y-STR markers in new generation Yfiler Plus (YFP) multiplex system. Performance of the 7 RM Y-STRs included in YFP is evaluated in central Indian population. We also report here a pilot study on genetic polymorphism using 13 Rapidly Mutating Y STRs (RM Y-STR) on Indian population. Forensic parameters including allele frequencies, gene diversities, discrimination capacity and Haplotype diversity will be calculated for both the amplification assays. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Pankaj Shrivastava, Rashed Alghafri, Reem Almheiri, Aditi Mishra, Hamdah Alfalasi, Sara Khouri, Ruksar Damji, Ram Kishan Kumawat, Sumit Kumar Choudhary, Shivani Dixit, Kamlesh Kaitholia, Hirak Ranjan Dash, Veena Ben Trivedi, Divya Shrivastava Source Type: research

Forensic evaluation of SE33 marker in Indian population using Global filer, Fusion 6 c and Panglobal autosomal STR multiplex
Capillary electrophoresis based forensic DNA typing using PCR with multiplex STR kit is gold standard now. But even one mismatch in the studied markers results in an inconclusive report. This deficiency is suggested to be taken care of by using alternative markers and/or by increasing the number of STR markers. Three new generation multiplex autosomal STR systems namely GlobalFiler â„¢ PCR Amplification Kit (Thermo Fisher Scientific Foster City, CA, USA), PowerPlex Fusion 6C (Promega, Madison, WI, USA), PanGlobal Human DNA Identification Kit (Health Gene Technologies Co., Ltd) incorporating new 20 CODIS and the SE33 marker...
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Pankaj Shrivastava, Kamlesh Kaitholia, Shivani Dixit, Hirak Ranjan Dash, Veena Ben Trivedi Source Type: research

Forensic characteristics of Tibeto-Burman-speaking Tibetans revealed by 50 InDels
In this study, 103 Tibeto-Burman-speaking Tibetan individuals were genotyped to investigate the effectiveness of this novel InDel kit. No significant deviation from Hardy-Weinberg equilibrium was observed at the 47 A-InDels after applying the Bonferroni correction. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Mengge Wang, Zheng Wang, Guanglin He, Hong Zhu, Yiping Hou Source Type: research

Performance of a massive parallel sequencing microhaplotypes assay on degraded dna
Massively parallel sequencing (MPS) has allowed to analyze a new type of forensic genetic marker, known as microhaplotypes (MHs). MHs appear to be useful for identification purposes, reconstruction of family relationships, ancestry prediction and DNA mixtures deconvolution. Moreover, MHs are potentially suitable for the analysis of degraded DNA samples.We designed a new panel of 29  MHs for MPS assay, with amplicons sizes below 180 bp and we investigated its effectiveness with low amounts of degraded samples. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Chiara Turchi, Filomena Melchionda, Mauro Pesaresi, Paolo Fattorini, Adriano Tagliabracci Source Type: research