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Genetic discrimination of the poisonous Urobotrya siamensis from the green-leaf vegetable Pak-wan
This study aimed to identify potential DNA markers to discriminate the 3 Pak-wan plant species. DNA sequence analysis of 7 DNA markers showed sequence polymorphisms (SNPs and InDels) in 5 potential DNA markers i.e. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 19, 2019 Category: Forensic Medicine Authors: Wanasphon Bunakkharasawat, Laksika Panok, Nathinee Panvisavas Source Type: research

Ancestry evaluation in a population sample of the Tunja city, Department of Boyac á - Colombia
Ancestry Informative Markers InDels (AIM-InDels) show high allele frequency variation between ancestral populations and are useful to estimate individual and population ancestry. This work determined the African, European and Native American admixture proportions in a population sample of 126 individuals from Tunja, Department of Boyac á, Colombia. This region is located close to the center of the country and has a diverse population. To estimate the African, European and Native American admixture proportions, in the sample there were used as parental populations, the genetic profiles of 319 individuals (Africans: 105, Eu...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Izquel Sanchez, Libardo Mendoza, Alma Luciel Rinc ón, Diana Patricia Aguirre, Juan José Builes Source Type: research

GENETIC IDENTIFICATION OF SPANISH CIVIL WAR VICTIMS. THE STATE OF THE ART IN CATALONIA (Northeastern Spain)
At the end of 2016 and under the initiative and funding of the “Direcció General de Memòria democràtica-Departament de Justícia” (Generalitat of Catalonia), it was decided to recover and identify the remains of people disappeared in Catalonia during and after the Spanish Civil War (1936–1939).Anthropology, archaeology, history and genetics are part of the global procedure. To achieve identification by genotyping, two different genetic approaches have been carried out: 1) the directed identification through kinship analysis of alleged living relatives, when there is a previous evidence (archaeological, anthropolog...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: S. Palomo-D íez, C. Gomes, A.M. López-Parra, C. Baeza-Richer, I. Cuscó, C. Raffone, E. García-Arumí, D.C. Vinueza-Espinosa, C. Santos, N. Montes, R. Rasal, O. Escala, J. Cuellar, E. Subirá, F. Casals, A. Malgosa, E. Tizzano, E. Tartera, G. Domenech, Source Type: research

Evaluation of two FTA card elutions with sterile vs distilled water
DNA extraction is frequently the starting point for all the experiments in biomedical and forensic research [1]. Nowadays, there are multiple commercial kits that allow carrying out the extraction rapidly, but often with a high financial cost. With the aim of presenting an alternative to these commercial kits, we have proposed two new DNA extraction methods using water.The extractions were carried out over FTA cards impregnated with blood, and using as extraction reagent two types of water. For the essays, it was compared the efficiency of using sterile water versus distilled water, with two objectives: 1) Finding out if t...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: B. G ómez, P. Mármol, C. Gomes, A. Hernández-Cordero, M. Martín-Arrebola, R. Rosell-Herrera, A.M. López-Parra, S. Palomo-Díez, E. Labajo-González, B Perea-Pérez, C. López-Matayoshi, C. Baeza-Richer, E. Arroyo-Pardo Source Type: research

Single step strategy for estimating unknown str allele frequencies in a population
Constant growth of forensic DNA databases requires new STR markers to be developed, tested and validated. Aiming to simplify the process of obtaining allele frequency distributions of new STR loci included in new megaplexes, a single step protocol was developed. This goal was achieved by pooling samples of genetically unrelated donors, previously genotyped (n  = 99), containing the same amount of DNA (total DNA = 4.5 ng/ul) as measured by fluorometry. Suitability of the method was tested by amplifying commercial kits: GlobalFiler (GF), Verifiler Express (VFE) and Y-filer plus (YFP). (Source: Forensic Science Inte...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Lucia Garrigos, Mariela Caputo, Daniel Corach Source Type: research

Specific m(i)RNA profiling from DNA eluates for body fluid identification
DNA analysis can yield information usable to identify individuals or to associate suspects, victims and crime scene to one another but does not support a contextualization of trace material. Knowing the composition of biological material from a crime scene, e.g. whether a stain contains saliva or semen, is complementary to DNA-based identification and can be essential in reconstructing the course of events in criminal investigations. Research in the forensic applications of RNA analysis has surged considerably during the last two decades and provided recent advancements for forensic casework. (Source: Forensic Science Inte...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Laiq-Jan Saidi, Linda Helena M üller, Burkhard Madea, Melanie Grabmüller Source Type: research

Re-evaluation of DNA Based Identification results of Victims of a terrorist attack 25 years later
In July 1994 there was a second terrorist attack against a Jewish institution in Buenos Aires city. It targeted the Argentine Israelite Mutual Association (AMIA) and the bomb toppled its building, killed 85 and hurt over 300 people. The Supreme Court ordered DFPS/SHDG to carry out the identification of the fragmented remains by means of DNA analysis. At that time five VNTRs, seven STRs including five autosomal, one X, and one on the Y chromosome, mtDNA sequencing (HVR I and II) and MVR were used. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: D. Corach, C. Vullo, A. Sala, M. Catelli, M. Caputo, M. Romero, S. Ginart, L. Garrig ós, L. Fonderbrider, C. Somigliana Source Type: research

Ancestry evaluation in a population sample of the tunja city, department of boyac Á - colombia
Ancestry Informative Markers InDels (AIM-InDels) show high allele frequency variation between ancestral populations and are useful to estimate individual and population ancestry. This work determined the African, European and Native American admixture proportions in a population sample of 126 individuals from Tunja, Department of Boyac á, Colombia. This region is located close to the center of the country and has a diverse population. To estimate the African, European and Native American admixture proportions, in the sample there were used as parental populations, the genetic profiles of 319 individuals (Africans: 105, Eu...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Izquel Sanchez, Libardo Mendoza, Alma Luciel Rinc ón, Diana Patricia Aguirre, Juan José Builes Source Type: research

Trial to search for mitochondrial DNA mutation associated with cancer detected by massively parallel sequencing
Impairment of mitochondrial function has long been suspected to contribute to the generation and progression of cancer. We performed DNA analysis of blood and cancer-affected organs in patients with cancer and situs inversus, which is considered to be a pre-cancerous condition. The total mtDNA genome of patients was analyzed and compared with data from healthy Japanese people. Long-range PCR was performed with Nextera preparation spanning the entire human mitochondrial genome (16,569 bp). Library sequencing on MiSeq (Illumina) was followed by data analysis with the mtDNA variant analyzer. (Source: Forensic Science Internat...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Hisanori Muramatsu, Katsuya Honda, Shigeru Akanuma, Fujio Ishizawa, Keishi Umino, Yayoi Iwabuchi, Naoto Mochizuki, Yukiko Sugano Source Type: research

Isoallelic frequency estimation for short tandem repeat markers from massive parallel sequencing data
We present a statistical model for estimating isoallele frequencies based on MPS data. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Mikkel Meyer Andersen, Torben Tvedebrink Source Type: research

The effect of time and environmental conditions on Touch DNA
This study investigated the influence of time between depositio n and recovery of Touch DNA, as well as the impact of temperature and humidity on a range of porous and non-porous surfaces. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Salem K. Alketbi, W. Goodwin Source Type: research

The effect of surface type, collection and extraction methods on Touch DNA
There are different variables that affect the success of Touch DNA recovery, including surface type, the collection method used and extraction techniques. This experiment investigated how a range of porous and non-porous surfaces, different DNA collection (cotton swab, nylon flocked swab and SceneSafe Fast ™ minitape) and extraction methods (PrepFiler Express BTA™ and QIAamp® DNA Investigator) affected touch DNA recovery. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Salem K. Alketbi, W. Goodwin Source Type: research

Capturing spermatozoa for str analysis of sexual assault cases using anti-sperm antibodies
DNA isolation in sexual assault cases is complicated by the presence of large numbers of female epithelial cells, which are often in vast excess when compared to spermatozoa. The two-step differential extraction has been a standard method for isolating the spermatozoa; however, new techniques in forensic science allow the use of precision techniques for capturing spermatozoa.We have used an immuno-magnetic bead-based technique for sperm cell separation. We identified two antibodies that were specific to spermatozoa: SP17 polyclonal antibody and SP10 Intra Acrosomal Protein monoclonal antibody. (Source: Forensic Science Int...
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Dina Alsalafi, William Goodwin Source Type: research

Molecular evolution and complete genome sequences in forensic analysis: Neisseria gonorrhoeae in a transmission case
Molecular epidemiology and phylogenetic analyses are frequently used in the investigation of viral transmission cases in forensic contexts. Here, we present the methods and results of the analysis of a bacterial transmission in an alleged child abuse case using complete genome sequences obtained by high-throughput sequencing (HTS) methods. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Carlos Franc és-Cuesta, Idoia de la Caba, Pedro Idígoras, Amparo Fernández-Rodríguez, David del Valle Pérez, José María Marimón, Fernando González-Candelas Source Type: research

Optimization of STR amplification down to single cell after DEPArrayTM Isolation
In this study we tested and compared three different commercially available kits, each at different cycling conditions, to identify the best conditions to obtain the most informative profiles from single cells or very small pools of cells, isolated from casework samples. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Valentina Meloni, Andrea Berti Source Type: research