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Cutaneous miRNAs expression in human hanging injuries
The determination of dermal injuries age is crucial in forensic practice. It helps forensic pathologists to find important clues such as the timing of the injury, the order of infliction, the survival time after injury and the relation between an injury and the event occurred.Our work evaluated one of the most frequent injuries detect in our casework records and represented by hanging injury.Samples included in the present work were represented by 40 hanging injuries and 10 control samples obtained from healthy cutaneous tissues. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: M. Fabbri, P. Frisoni, M. Marti, L. Alfieri, E. Calabrese, C. Marini, R. Marino, R.M. Gaudio, M. Neri Source Type: research

NOS1AP SNPs RELATED TO SUDDEN ARRHYTHMIC DEATH SYNDROME
Between 12-20% of all Sudden Cardiac Death (SDC) occurs in individuals without previous symptoms of cardiac disease, which is known as Sudden Arrhythmic Death Syndrome (SADS). Recent studies identified the NOS1AP gene as new genetic marker to increase the risk of SDC. Here, we analyzed five SNPs of the NOS1AP gene by PCR-HRM in order to evaluate its ability as diagnostic and predictive biomarkers of the SADS. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: Tamara Kleinbielen, Mar ía Pilar Hernández-Sierra, Endika Prieto-Fernández, Felix Olasagasti, Rubén sevillano, Marian M. De Pancorbo Source Type: research

The EUROFORGEN NAME AmpliseqTM custom panel: a second tier panel developed for differentiation of individuals from the Middle East/North Africa
The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq ™ custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5™ (Thermo Fisher Scientific). (Source: Forensic Scienc...
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: D.M. Truelsen, V. Pereira, C. Phillips, N. Morling, C. B ørsting Source Type: research

The impact of ignoring inbreeding in pairwise kinship evaluations
Inbreeding is often ignored in forensic kinship inference, either because the exact genealogies are unknown, or because current methods are inadequate for inbred pedigrees. In many situations, this may be a reasonable simplification, but in other, it can lead to wrong inference of relatedness between two individuals. In order to quantify the impact of ignoring inbreeding, we simulate marker data for a selection of inbred pedigrees, and subject them to pairwise kinship inference using standard noninbred assumptions. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: Hilde Kjelgaard Brustad, Thore Egeland Source Type: research

Is Genomic DNA extracted and stored at -20 C for long time useful in forensic field?
With the progress of technologies, forensic genetic laboratories have been more frequently involved in "cold case". The aim of this study was to understand whether the DNA extracted and stored for a long time at ?20?C could be useful for new analyses in order to identify the perpetrator of unsolved crimes, especially when evidence is no longer available. We selected 120 DNA samples obtained from evidence collected at crime scene between 2001 and 2010: they were quantified again using Realtime PCR and the Plexor HY System kit. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: Giulia Sguazzi, Flavia Lovisolo, Sarah Gino Source Type: research

The impact of the Pr m treaty on the Portuguese forensic DNA databaseA brief review
The Treaty of Pr m signed on 27 May 2005, by seven Member States of European Union (EU), sought to improve cross-border security cooperation in combating terrorism, cross-border crime and illegal immigration. For this purpose, provisions were set for automated exchanges of data regarding DNA, fingerprints and vehi cle registration plates. Later, other Member States expressed their intention to accede to the Prm Convention, including Portugal.Portuguese forensic DNA profiles database was implemented in 2010, although only in 2015 Portugal started the automated DNA data exchange with other countries. (Source: Forensic Scie...
Source: Forensic Science International: Genetics Supplement Series - October 31, 2019 Category: Forensic Medicine Authors: P. Brito, A.M. Bento, N. Gouveia, L. Sampaio, F. Balsa, V. Lopes, M. S o Bento, P. Cunha, A. Serra, M.J. Porto Source Type: research

The impact of the Pr üm treaty on the Portuguese forensic DNA database—A brief review
The Treaty of Pr üm signed on 27 May 2005, by seven Member States of European Union (EU), sought to improve cross-border security cooperation in combating terrorism, cross-border crime and illegal immigration. For this purpose, provisions were set for automated exchanges of data regarding DNA, fingerprints and vehi cle registration plates. Later, other Member States expressed their intention to accede to the Prüm Convention, including Portugal.Portuguese forensic DNA profiles database was implemented in 2010, although only in 2015 Portugal started the automated DNA data exchange with other countries. (Source: Forensic Sc...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: P. Brito, A.M. Bento, N. Gouveia, L. Sampaio, F. Balsa, V. Lopes, M. S ão Bento, P. Cunha, A. Serra, M.J. Porto Source Type: research

Is Genomic DNA extracted and stored at -20 °C for long time useful in forensic field?
With the progress of technologies, forensic genetic laboratories have been more frequently involved in "cold case". The aim of this study was to understand whether the DNA extracted and stored for a long time at −20 °C could be useful for new analyses in order to identify the perpetrator of unsolved crimes, especially when evidence is no longer available. We selected 120 DNA samples obtained from evidence collected at crime scene between 2001 and 2010: they were quantified again using Realtime PCR and the “Plexor HY System” kit. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Giulia Sguazzi, Flavia Lovisolo, Sarah Gino Source Type: research

A machine learning model to predict the origin of forensically relevant body fluids
More often than not, DNA profiling alone is not sufficient to accurately determine the nature of a crime. In such cases, the identification of the cellular origin and composition of crime scene related traces, shortly termed as body fluid identification (BFI) can provide contextual information with regard to the circumstances in which the crime unfolded.Our approach uses a targeted mRNA-Sequencing protocol for body fluid identification, based on a multiplexed panel of highly specific biomarkers corresponding to the five categories of forensically relevant body fluids: blood, saliva, semen, vaginal secretions and menstrual ...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Diana Iacob, Angelika F ürst, Thorsten Hadrys Source Type: research

Resolution of mtDNA Mixtures Using a Probe Capture Next Generation Sequencing System and Custom Analysis Software
To analyze crime scene evidence with degraded DNA, we have designed a probe capture Next Generation Sequencing system for targeted enrichment for both nuclear SNP markers and for the entire mitochondrial DNA genome. This probe capture NGS system was used to capture and sequence both nuclear SNPs and mtDNA markers from the same DNA shotgun library. Version 1 of the SNP panel (SNPv1.0; 451 SNPs) includes identity informative, ancestry informative, phenotypic informative, haploid chromosomal, tri-and tetra-allelic and microhaplotype SNPs. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Shelly Shih, Jessica Lim, Mary Wisner, Henry Erlich, Cassandra Calloway Source Type: research

Genetic and chromosomal variation-caused inconsistencies in two parental tests
Two special cases were reported where inconsistency between children and the fathers were observed at FGA locus. Regular additional STR tests were performed and no more inconsistency was observed in the first case. Through the following TA cloning and sequencing, the first case turned to be a two-step mutation between child and father. In the second case, however, one more inconsistency was found in the additional test at D4S2366, which was on Chr. 4 as well. Then, seven randomly selected STR-loci on Chromosome 4 were analyzed, indicating a possible maternal uniparental disomy (UPD) in the child with normal phenotype. (Sou...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Chen Li, Yifan Li, Li Jia, Chong Chen, Shi Yan, Chuguang Chen, Yacheng Liu, He Ren Source Type: research

Multiplex DNA methylation profiling by ARMS-PCR for body fluid identification
A set of CpG sites were detected and evaluated to identify body fluids based on DNA methylation using the amplification refractory mutation system-PCR (ARMS-PCR). In this research, two multiplex DNA methylation reactions composed of four promising CpG sites were used for the identification of forensic regular body fluids. ARMS-specific primers were used to amplify the CpG sites and the methylation values of each CpG site were detected by capillary electrophoresis. The DNA methylation profiling of four highly informative candidate CpG sites was consistent with previously reported results. (Source: Forensic Science Internati...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Huan Tian, Yu Tan, Zhilong Li, Duo Peng, Weibo Liang, Lin Zhang, Dan Chen, Tao Feng, Qian Feng, Mengna Wu, Jian Yu, Peng Bai Source Type: research

The impact of the pr Üm treaty on the portuguese forensic dna database—a brief review
The Treaty of Pr üm signed on 27 May 2005, by seven Member States of European Union (EU), sought to improve cross-border security cooperation in combating terrorism, cross-border crime and illegal immigration. For this purpose, provisions were set for automated exchanges of data regarding DNA, fingerprints and vehi cle registration plates. Later, other Member States expressed their intention to accede to the Prüm Convention, including Portugal.Portuguese forensic DNA profiles database was implemented in 2010, although only in 2015 Portugal started the automated DNA data exchange with other countries. (Source: Forensic Sc...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: P. Brito, A.M. Bento, N. Gouveia, L. Sampaio, F. Balsa, V. Lopes, M. S ão Bento, P. Cunha, A. Serra, M.J. Porto Source Type: research

Y-STR Chromosome Structure Variations: Incidence and Implications
Abstract (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Walter Ruben Bozzo, Mariana Herrera Pi ñero Source Type: research

The EUROFORGEN NAME Ampliseq custom panel: A second tier panel developed for differentiation of individuals from the Middle East/North Africa
The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5 (Thermo Fisher Scientific). (Source: Forensic Science In...
Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: D.M. Truelsen, V. Pereira, C. Phillips, N. Morling, C. B rsting Source Type: research