Multiplex DNA methylation profiling by ARMS-PCR for body fluid identification

A set of CpG sites were detected and evaluated to identify body fluids based on DNA methylation using the amplification refractory mutation system-PCR (ARMS-PCR). In this research, two multiplex DNA methylation reactions composed of four promising CpG sites were used for the identification of forensic regular body fluids. ARMS-specific primers were used to amplify the CpG sites and the methylation values of each CpG site were detected by capillary electrophoresis. The DNA methylation profiling of four highly informative candidate CpG sites was consistent with previously reported results.

Source: Forensic Science International: Genetics Supplement Series - October 30, 2019 Category: Forensic Medicine Authors: Huan Tian, Yu Tan, Zhilong Li, Duo Peng, Weibo Liang, Lin Zhang, Dan Chen, Tao Feng, Qian Feng, Mengna Wu, Jian Yu, Peng Bai Source Type: research