Forensic Science International: Genetics Supplement Series 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Study of Y chromosome markers with forensic relevance in Lisbon immigrants from African countries Allelic variants study
Due to the haploid nature of the human male determinant chromosome, Y chromosome Short Tandem Repeats (Y-STRs) are interesting genetic markers for the identification of potential suspects in certain forensic investigations. Furthermore, being a haploid chromosome, the presence of more than one fragment size in Y-STR analysis is often indicative of a mixture of male DNA. Certain events during DNA replication may lead to the appearance of allelic variants in certain markers that could result in duplications, microvariants or null alleles. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 27, 2019 Category: Forensic Medicine Authors: ngela Dente, Antnio Amorim, Helosa Afonso Costa, Maria Joo Porto, Eugnia Cunha, Francisco Corte Real, Cludia Vieira da Silva Source Type: research
Standardization in forensic genetics A multi-faceted challenge in Poland
Extremely dynamic development of forensic genetics requires cooperation of the professional bodies and institutions in order to make the best use of technological progress for the public good. The need for the high quality of DNA testing for justice and law enforcement in Poland forces the necessity of standardization in this area based on norms and legal Polish as well as European requirements. Among them the most crucial is the implementation of PN-EN ISO/IEC 17025 standard and the fulfillment of the requirements of the DAB-10 (PCA) document. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 27, 2019 Category: Forensic Medicine Authors: Renata Jacewicz, Sasitaran Iyavoo Source Type: research
Standardization in forensic genetics —A multi-faceted challenge in Poland
Extremely dynamic development of forensic genetics requires cooperation of the professional bodies and institutions in order to make the best use of technological progress for the public good. The need for the high quality of DNA testing for justice and law enforcement in Poland forces the necessity of standardization in this area based on norms and legal Polish as well as European requirements. Among them the most crucial is the implementation of PN-EN ISO/IEC 17025 standard and the fulfillment of the requirements of the DAB-10 (PCA) document. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 26, 2019 Category: Forensic Medicine Authors: Renata Jacewicz, Sasitaran Iyavoo Source Type: research
Study of genetic markers with medico-legal and forensic interest in lisbon ’s population (preliminary results)
In this study, samples from 176 individuals, from both genders and from unrelated individuals comprised in forensic investigations in progress at Instituto Nacional De Medicina Legal e Ciências Forenses (INMLCF), were analyz ed with the Investigator HDplex Kit (Qiagen). (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 26, 2019 Category: Forensic Medicine Authors: Diogo Rodrigues, Cl áudia Vieira da Silva, Heloísa Afonso Costa, Maria João Porto, Eugénia Cunha, Francisco Corte Real, António Amorim Source Type: research
Study of Y chromosome markers with forensic relevance in Lisbon immigrants from African countries – Allelic variants study
Due to the haploid nature of the human male determinant chromosome, Y chromosome Short Tandem Repeats (Y-STRs) are interesting genetic markers for the identification of potential suspects in certain forensic investigations. Furthermore, being a haploid chromosome, the presence of more than one fragment size in Y-STR analysis is often indicative of a mixture of male DNA. Certain events during DNA replication may lead to the appearance of allelic variants in certain markers that could result in duplications, microvariants or null alleles. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 26, 2019 Category: Forensic Medicine Authors: Ângela Dente, António Amorim, Heloísa Afonso Costa, Maria João Porto, Eugénia Cunha, Francisco Corte Real, Cláudia Vieira da Silva Source Type: research
Standardization in forensic genetics - a multi-faceted challenge in Poland
Extremely dynamic development of forensic genetics requires cooperation of the professional bodies and institutions in order to make the best use of technological progress for the public good. The need for the high quality of DNA testing for justice and law enforcement in Poland forces the necessity of standardization in this area based on norms and legal Polish as well as European requirements. Among them the most crucial is the implementation of PN-EN ISO/IEC 17025 standard and the fulfillment of the requirements of the DAB-10 (PCA) document. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 26, 2019 Category: Forensic Medicine Authors: Renata Jacewicz, Sasitaran Iyavoo Source Type: research
Estimation of genotyping errors of str markers in dogs and wolves
This article will discuss the estimation of genotyping errors for Mini-DogFiler panel A and B in group of wolfs (n = 39) where the source material for DNA isolation were faeces and control group of dogs (n = 170), represented by Czechoslovakian wolfdog breed (CSW), where DNA was obtained from buccal swabs.DNA samples isolated from buccal swabs had a very low frequency of genotyping error estimated as me an error rate per allele (ea = 0.09 %), mean error per locus (el = 0.18 %) and error rate per multilocus genotype (eobs = 2.17 %). (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 25, 2019 Category: Forensic Medicine Authors: Radka Štikarová, Jakub Vašek, Daniela Čílová, Pavel Vejl Source Type: research
Internal Validation of GlobalFilerTM Kit Using Reduced Reaction Volume
For approximately two decades, Microsatellites or Short Tandem Repeat (STR) markers have been the backbone for human identification testing in the forensic field. The expansion of STR multiplex kits can improve global sharing of STR profiles, rapid DNA typing, and DNA typing using high throughput sequencing Flores et al. (2014). GlobalFilerTM kit is highly sensitive and has extremely high power of discrimination. An internal validation of GlobalFiler ™ Kit was carried out to test the robustness of this kit through a range of internal validation studies including half volume reaction, reproducibility, sensitivity, specifi...
Source: Forensic Science International: Genetics Supplement Series - November 20, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research
A study of old Serbian skeletal remains using ForenSeq DNA Signature kit
In this study Illumina ForenSeq DNA Signature Prep kit was tested to determine if MPS offers a more comprehensive evaluation of degraded samples than the traditional fragment analysis/capillary electrophoresis based method (Athey, 2006). The use of MPS could reduce th e analysis time and augment the identification of human remains. In this context we aimed to analyse bone/tooth samples using ForenSeq DNA Signature kit. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 18, 2019 Category: Forensic Medicine Authors: Eida Almohammed, Dragana Zgonjanin, Saleh Alsaadi, Sibte Hadi Source Type: research
A Study of old Serbian Skeletal Remains using ForenSeq DNA Signature ™ Kit
In this study Illumina ForenSeq™ DNA Signature Prep kit was tested to determine if MPS offers a more comprehensive evaluation of degraded samples than the traditional fragment analysis/capillary electrophoresis based method (Athey, 2006). The use of MPS would therefore reduce the analysis time and augment the identification of human remains. In this context we aimed to analyse the hard tissue degraded samples using ForenSeq™ DNA Signature kit. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 17, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research
A study of skeletal remains using GlobalFiler
DNA analysis from human remains is of immense relevance in missing persons identification and disaster victim identification (DVI). DNA degrades gradually in hard tissues, such as bones and teeth and remains somewhat safe from the effects of high temperature, humidity, pH, geochemical properties of the soil, and microorganisms. . The GlobalFiler kit simultaneously amplifies and detects 21 autosomal loci including CODIS extended set of STR loci. The kits was designed to combine all 21 autosomal STR loci along with a novel male speci?c Y insertion/deletion marker the sex-determining marker, amelogenin. (Source: Forensic Sc...
Source: Forensic Science International: Genetics Supplement Series - November 15, 2019 Category: Forensic Medicine Authors: E. Almohammed, Zgonjanin Dragana, S. Hadi Source Type: research
Analysis of 55 Kidd ancestry SNPs in Qatari population using ForenSeq Universal software & STRUCTURE software
SNPs are good predictors of ethnicity and serval panels have been published [1]. The ForenSeq Signature kit (Illumina) offers coverage of 230 different markers including 55 ancestry SNPs (AISNPs). The ForenSeq Universal Analysis Software (UAS) brings the capability to analyse the sequencing data, represent results and accomplish statistical estimates of biogeographic ancestry. The ancestry prediction results in UAS are based on Principal Component Analysis (PCA) collected on several reference populations comprised in the 1000 Genomes project. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 14, 2019 Category: Forensic Medicine Authors: E. Almohammed, S. Hadi Source Type: research
A Study of Skeletal Remains using GlobalFiler ™
DNA analysis from human remains is of immense relevance in missing persons identification and disaster victim identification (DVI). DNA degrades gradually in hard tissues, such as bones and teeth under a high temperature, humidity, pH, geochemical properties of the soil, the presence of microorganisms and all other factors that affect the preservation of DNA in skeletal remains. The GlobalFiler ™ kit simultaneously amplifies and detects 21 autosomal loci including CODIS extended set of STR loci. The kits was designed to combine all 21 autosomal STR loci along with a novel male specific Y insertion/deletion marker the se...
Source: Forensic Science International: Genetics Supplement Series - November 14, 2019 Category: Forensic Medicine Authors: E. Almohammed, Zgonjanin Dragana, S. Hadi Source Type: research
Analysis of 55 Kidd Ancestry SNP of Qatari population using Structure & ForenSeq Universal Software
SNPs are good predictors of ethnicity and serval panels have been published [1]. The ForenSeq Signature kit (Illumina) offers coverage of 230 different markers including 55 ancestry SNPs (AISNPS). The ForenSeq Universal Analysis Software (UAS) brings the capability to analyse the sequencing data, represent results and accomplish statistical estimates of biogeographic ancestry. The ancestry prediction results in UAS are based on Principal Component Analysis (PCA) collected on several reference populations comprised in the 1000 Genomes project. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 14, 2019 Category: Forensic Medicine Authors: E. Almohammed, S. Hadi Source Type: research
Forensic evaluation of VeriFiler Plus 6-dye chemistry kit composed of 23 loci with casework samples
In this study, I have evaluated a six-dye STR multiplex assay composed of 23 autosomal STR loci, 1 insertion/deletion polymorphic marker on the Y chromosome, Amelogenin and two internal quality control markers (IQCS and IQCL). This Internal validation indicated that VeriFilerTM Plus PCR amplification kit was reproducible, sensitive, accurate and robust. (Source: Forensic Science International: Genetics Supplement Series)
Source: Forensic Science International: Genetics Supplement Series - November 14, 2019 Category: Forensic Medicine Authors: Naeema Al Janaahi, Rashed Al Ghafri, Synan Abu Qamar Source Type: research
