Internal Validation of GlobalFilerTM Kit Using Reduced Reaction Volume

For approximately two decades, Microsatellites or Short Tandem Repeat (STR) markers have been the backbone for human identification testing in the forensic field. The expansion of STR multiplex kits can improve global sharing of STR profiles, rapid DNA typing, and DNA typing using high throughput sequencing Flores et al. (2014). GlobalFilerTM kit is highly sensitive and has extremely high power of discrimination. An internal validation of GlobalFiler ™ Kit was carried out to test the robustness of this kit through a range of internal validation studies including half volume reaction, reproducibility, sensitivity, specificity, stability, mixture, analytical threshold, sensitivity& stochastic threshold, heterozygous balance& stutter threshold studies in accordance with SGWDAM guidelines Scientific Working Group on DNA Analyis Methods Y-STR Subcommittee (2004).

Source: Forensic Science International: Genetics Supplement Series - November 20, 2019 Category: Forensic Medicine Authors: Eida Almohammed Source Type: research