Trial to search for mitochondrial DNA mutation associated with cancer detected by massively parallel sequencing
Impairment of mitochondrial function has long been suspected to contribute to the generation and progression of cancer. We performed DNA analysis of blood and cancer-affected organs in patients with cancer and situs inversus, which is considered to be a pre-cancerous condition. The total mtDNA genome of patients was analyzed and compared with data from healthy Japanese people. Long-range PCR was performed with Nextera preparation spanning the entire human mitochondrial genome (16,569 bp). Library sequencing on MiSeq (Illumina) was followed by data analysis with the mtDNA variant analyzer.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Hisanori Muramatsu, Katsuya Honda, Shigeru Akanuma, Fujio Ishizawa, Keishi Umino, Yayoi Iwabuchi, Naoto Mochizuki, Yukiko Sugano Source Type: research
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