Single step strategy for estimating unknown str allele frequencies in a population

Constant growth of forensic DNA databases requires new STR markers to be developed, tested and validated. Aiming to simplify the process of obtaining allele frequency distributions of new STR loci included in new megaplexes, a single step protocol was developed. This goal was achieved by pooling samples of genetically unrelated donors, previously genotyped (n  = 99), containing the same amount of DNA (total DNA = 4.5 ng/ul) as measured by fluorometry. Suitability of the method was tested by amplifying commercial kits: GlobalFiler (GF), Verifiler Express (VFE) and Y-filer plus (YFP).

Source: Forensic Science International: Genetics Supplement Series - October 18, 2019 Category: Forensic Medicine Authors: Lucia Garrigos, Mariela Caputo, Daniel Corach Source Type: research