DNA extraction of burnt bone and teeth casework samples using bead-beating homogenization technique

Sample disruption was a necessary step for DNA isolation. Bone and teeth were useful biological sources particular in human remains and advance decomposed bodies. The compact bone and teeth required several preparation steps prior to analyzing process. However, the methods in standard protocol were laborious and time consuming. An alternating pulverization, bead beating homogenizer, was purposed in its effectiveness for forensic casework. (1) Here, we applied this technique to the burnt cracked bone and tooth that recovered from house fire for forensic DNA analysis.

Source: Forensic Science International: Genetics Supplement Series - October 18, 2022 Category: Forensic Medicine Authors: P. Varrathyarom, T. Sathirapatya, W. Worrapitirungsi, K. Vongpaisarnsin Source Type: research