Assessing dna recovery from highly degraded skeletal remains by using silica-based extraction methods

Through this study, we extracted human DNA as for five different type of skeletal remains: petrous bone, tooth pulp cavity, tooth cementum, rib and at least two different upper limbs bones (radial, ulna, metacarpal or phalange) from five individuals (5-12th centuries AD) by using four extraction methods; three based on DNA adsorption to silicon dioxide (silica) particles, Non-Columns Silica (NCSi), Silica-HE Spin Columns (SiHEC), Silica-XS Spin Columns (SiXSC) and the traditional organic extraction method (P-Chl) (N:100 DNA extracts).
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Source Type: research