Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories

Sodium hypochlorite is an efficient reagent for removal of unwanted DNA from laboratory surfaces. Here, we tested two different chlorine wipes and compared their performance to a 0.9-1.8% hypochlorite solution. WipeClean Chlorine Disinfection wipes contain>0.1g sodium hypochlorite/kg, whereas WetWipe Chlorine Desinfection wipes contain>1000 ppm active chlorine. Clean surfaces were contaminated with 10 µl 0.5ng/µl of massively parallel sequencing libraries. The DNA was dried and left for 45minutes before any treatment.

Source: Forensic Science International: Genetics Supplement Series - October 7, 2022 Category: Forensic Medicine Authors: Marie-Louise Kampmann, Bo Thisted Simonsen, Claus B ørsting Source Type: research