Recovery of DNA from bone without demineralization

This study assessed the performance of five different DNA extraction methods for the recovery of DNA from bone: ChargeSwitch ® gDNA Plant Kit, DNA IQTM System Kit, DNeasy® Blood& Tissue Kit, PrepFiler ® BTA Forensic DNA Extraction Kit and phenol-chloroform-isoamyl alcohol. DNA was extracted from pig rib and femur bones that was fresh, had undergone surface decomposition for three months, and had undergone surface decomposition for one year. Extracted DNA was analyzed using real-time PCR and ampl ification of an in-house PCR multiplex that assessed the quality and quantity of DNA and for the presence of inhibitors.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Source Type: research